FastQCFastQC Report
Fri 10 Feb 2017
SRR3224523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362194
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.2727819897623925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.25455971109405456No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC7620.21038449007990193No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA7130.1968558286443177No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG6970.19243830654290242No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA6800.1877446893101487No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT4990.13777147053788855No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA4470.12341452370828893No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA4210.11623605029348913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.11237071845475077No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT4010.11071414766672004No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC3940.10878148174735087No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA3700.10215519859522798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.065.35611
AAACCGT1350.063.323126
AACCGTG1450.058.956017
CCGTGCG1550.055.167629
ACCGTGC1800.050.1308028
GTATCAA15100.045.6430361
TGCGCGG1150.037.18331512-13
GACTATC651.3276479E-436.532577
ATCAACG19000.036.494113
TCAACGC19650.035.2869264
TATCAAC21250.033.0770152
CAACGCA21750.032.098275
GTGCGCG1350.031.6746810-11
GTAATAC600.0039505331.6615583
TGGTATC3450.031.6615582
AACGCAG22300.031.3066086
ACGCAGA23250.029.8231477
CGCAGAG23500.029.5058788
AAGACCG2450.029.076945
GTACATG27700.028.6563031
Produced by FastQC (version 0.11.5)