##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224523_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362194
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36525729305289	34.0	31.0	34.0	31.0	34.0
2	32.50125347189628	34.0	31.0	34.0	31.0	34.0
3	32.63084700464392	34.0	31.0	34.0	31.0	34.0
4	35.92690657492946	37.0	37.0	37.0	35.0	37.0
5	35.95917381292898	37.0	37.0	37.0	35.0	37.0
6	36.075106158578	37.0	37.0	37.0	35.0	37.0
7	36.035340176811324	37.0	37.0	37.0	35.0	37.0
8	36.07116075915118	37.0	37.0	37.0	35.0	37.0
9	37.81759499052994	39.0	39.0	39.0	35.0	39.0
10-11	37.89368266729984	39.0	39.0	39.0	37.0	39.0
12-13	37.89628072248574	39.0	39.0	39.0	37.0	39.0
14-15	39.393975328139064	41.0	40.0	41.0	37.0	41.0
16-17	39.232490046770515	41.0	40.0	41.0	36.5	41.0
18-19	39.35308840013915	41.0	40.0	41.0	37.0	41.0
20-21	39.42123005902914	41.0	40.0	41.0	37.0	41.0
22-23	39.36637823928613	41.0	40.0	41.0	37.0	41.0
24-25	39.310398570931596	41.0	40.0	41.0	36.5	41.0
26-27	39.157829781829626	41.0	40.0	41.0	36.0	41.0
28-29	39.06345632451117	41.0	39.5	41.0	35.0	41.0
30-31	38.93995483083651	41.0	39.0	41.0	35.0	41.0
32-33	38.688314825756365	41.0	39.0	41.0	35.0	41.0
34-35	38.65348404446236	41.0	39.0	41.0	35.0	41.0
36-37	38.617540599789066	41.0	39.0	41.0	35.0	41.0
38-39	38.47235735545039	41.0	38.5	41.0	35.0	41.0
40-41	38.356292484138336	40.0	38.0	41.0	35.0	41.0
42-43	38.17131840947117	40.0	38.0	41.0	34.0	41.0
44-45	37.91378101238563	40.0	38.0	41.0	33.0	41.0
46-47	37.62932986189722	40.0	37.5	41.0	33.0	41.0
48-49	37.330679138804065	40.0	37.0	41.0	32.0	41.0
50-51	36.030019823630425	38.5	35.5	40.0	29.5	40.5
52-53	36.32671855414502	39.0	35.5	40.0	30.5	40.5
54-55	36.75509257469754	40.0	35.5	41.0	31.0	41.0
56-57	36.608038785844045	39.0	35.0	41.0	31.0	41.0
58-59	36.467130874614156	39.0	35.0	41.0	31.0	41.0
60-61	36.22452194128009	39.0	35.0	41.0	30.0	41.0
62-63	35.95142244211665	38.5	35.0	41.0	30.0	41.0
64-65	35.70207816805359	37.5	35.0	41.0	29.0	41.0
66-67	35.43955587337173	37.0	35.0	40.0	29.0	41.0
68-69	35.13097538887999	36.5	35.0	40.0	29.0	41.0
70-71	34.82698498594675	36.0	35.0	39.0	29.0	41.0
72-73	34.496420426622194	35.5	35.0	39.0	28.0	41.0
74-75	34.18990927513984	35.0	34.0	38.5	28.0	40.5
76-77	33.86227822658575	35.0	34.0	37.0	27.0	39.5
78-79	33.61810245338134	35.0	34.0	37.0	27.0	39.0
80-81	33.334051640833366	35.0	34.0	36.0	27.0	39.0
82-83	33.076124949612634	35.0	34.0	36.0	26.0	37.5
84-85	32.8537275603682	35.0	34.0	36.0	26.0	37.0
86-87	32.641778991369264	35.0	34.0	35.0	26.0	37.0
88-89	32.48905144756677	35.0	34.0	35.0	25.5	36.0
90-91	32.32998199859744	35.0	33.0	35.0	25.0	36.0
92-93	32.20579026709443	35.0	33.0	35.0	25.0	36.0
94-95	32.067770310938336	35.0	33.0	35.0	25.0	36.0
96-97	31.94689724291402	35.0	33.0	35.0	24.0	35.0
98-99	31.893765495839247	35.0	33.0	35.0	24.0	35.0
100-101	30.756365373253008	34.5	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	31.0
8	95.0
9	120.0
10	142.0
11	327.0
12	518.0
13	604.0
14	634.0
15	634.0
16	760.0
17	979.0
18	1275.0
19	1581.0
20	2083.0
21	2982.0
22	3721.0
23	3374.0
24	2595.0
25	2222.0
26	1905.0
27	2072.0
28	2263.0
29	2490.0
30	3132.0
31	3978.0
32	5091.0
33	7248.0
34	11668.0
35	21373.0
36	46546.0
37	87906.0
38	108933.0
39	32874.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.88896969160706	18.968668515604907	12.55941534471269	22.582946448075344
2	16.636388233929882	20.269800162343937	38.97220826407947	24.121603339646708
3	19.89099764214758	27.840328663644343	28.783193537165165	23.48548015704291
4	12.510974781470704	16.783546938933277	38.81041651711514	31.895061762480882
5	12.69209318762873	39.88525486341574	33.46714744032204	13.955504508633496
6	28.702297663682998	37.218175894686276	19.430194867943698	14.64933157368703
7	26.4844254736412	32.72362325162758	22.227866833796252	18.56408444093497
8	26.770460029707838	33.519329420145006	20.652191919247695	19.05801863089946
9	27.999911649557973	14.548280755617155	20.479080271898486	36.97272732292639
10-11	25.71577662799494	25.786595029183257	27.513293980573945	20.984334362247857
12-13	26.544200069575975	24.1140107235349	27.62166131962429	21.720127887264837
14-15	24.066412388730388	25.17089189689315	25.71857453372405	25.04412118065241
16-17	22.442805457742832	27.874024885030718	26.93686462979821	22.74630502742824
18-19	22.24798259034847	26.721357201641542	28.799109633303694	22.231550574706297
20-21	24.282038781989755	24.898114760550698	28.68806613460314	22.131780322856404
22-23	23.895734335285667	26.182451099569015	27.835948723615868	22.08586584152945
24-25	22.638073317860304	25.9909619465642	28.73123457932183	22.63973015625367
26-27	22.47828511792023	26.67313649591103	28.734600794049598	22.11397759211914
28-29	22.27507910125513	26.647459648696554	28.8265404727853	22.250920777263016
30-31	22.5125209142062	25.967713435341285	28.941396047422103	22.57836960303042
32-33	22.33195469831085	26.152421078206707	29.086622086506125	22.429002136976315
34-35	22.888562483089174	26.011612561224094	28.643765495839247	22.456059459847484
36-37	22.32545744757234	25.982690725122747	29.015956124799096	22.675895702505812
38-39	22.389100266722625	26.40705848570024	28.384561012110197	22.81928023546693
40-41	22.994112003092408	25.843957727909654	28.860020293916655	22.30190997508128
42-43	22.860542874734595	26.126433322381224	28.494573185378663	22.518450617505515
44-45	22.835782774069294	26.199236520022367	28.107798402628692	22.85718230327965
46-47	22.873515298431226	26.079531963533352	27.72864818301794	23.318304555017477
48-49	22.417406141460102	26.354798809477792	27.88436583709283	23.343429211969276
50-51	22.63924857949055	26.85729194851378	27.13973726787302	23.363722204122652
52-53	23.249556867314205	26.88434927138495	26.535641120504483	23.330452740796368
54-55	22.995963489179832	26.348586669022676	27.113922373092876	23.54152746870462
56-57	23.06954284168153	26.896083314466836	26.756103082878234	23.278270760973403
58-59	23.32435421450199	26.81792638963485	26.955581666029243	22.902137729833917
60-61	23.22117442390269	27.037706932497553	26.82576939539122	22.915349248208543
62-63	23.054478469322376	26.670588365259274	27.362547226088687	22.912385939329667
64-65	23.63313942889342	26.092438831711938	26.806460166724673	23.46796157266997
66-67	22.86381754895612	26.523773559896778	26.43347586745845	24.178933023688646
68-69	22.94267712883151	26.722695571986282	27.253212366853123	23.081414932329082
70-71	23.452348741282297	26.151316697681352	26.988437135899545	23.407897425136806
72-73	23.21076550136115	26.518799317492835	27.098737140869254	23.17169804027676
74-75	23.09038802409758	26.50941208302733	27.11267994500185	23.28751994787324
76-77	23.117169251837414	26.19162658685677	26.727941379481713	23.963262781824106
78-79	23.182981097011226	25.675397672437477	27.16060291849665	23.98101831205464
80-81	22.871661565321162	26.85264478060531	26.60402324887568	23.671670405197847
82-83	22.998538766873928	26.895528737124437	26.852990003397576	23.252942492604063
84-85	22.872056173491583	26.26428329777612	27.339699110034037	23.52396141869826
86-87	22.514130380732954	27.30713342504959	26.823827440840255	23.354908753377202
88-89	23.079876104557147	26.47070601898202	26.701318356880186	23.748099519580645
90-91	22.795518423828113	26.35259004842709	26.978083568474354	23.873807959270447
92-93	22.728301834697735	26.668967516600635	27.28481300992587	23.317917638775764
94-95	22.966697405257953	26.293781785451998	27.225050663456603	23.51447014583345
96-97	23.207866502482094	26.67120382999166	27.015356411205044	23.1055732563212
98-99	22.315312554356378	27.01750171869694	27.079071102975483	23.588114623971197
100-101	22.959565576464673	26.480556177028696	26.78894495957663	23.770933286930003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	3.5
3	4.0
4	10.0
5	14.0
6	16.5
7	28.5
8	45.0
9	71.5
10	79.0
11	88.5
12	124.5
13	151.0
14	177.0
15	202.5
16	228.5
17	238.0
18	274.5
19	329.5
20	364.0
21	401.5
22	458.0
23	570.5
24	733.5
25	947.5
26	1292.0
27	1779.5
28	2214.5
29	2722.0
30	3414.5
31	4226.0
32	5257.0
33	6394.0
34	7656.0
35	8768.0
36	9942.0
37	10833.0
38	10802.5
39	10889.5
40	10873.0
41	10595.0
42	10357.5
43	10395.5
44	10802.0
45	11668.5
46	12581.0
47	12435.5
48	12651.5
49	13646.0
50	14707.5
51	14330.0
52	13352.0
53	13848.0
54	14373.0
55	14115.0
56	12212.0
57	10142.5
58	9186.0
59	8542.0
60	7300.5
61	5515.5
62	4439.5
63	3251.0
64	2003.5
65	1462.5
66	1237.0
67	1059.5
68	887.5
69	650.0
70	425.0
71	294.5
72	235.5
73	184.5
74	150.5
75	124.0
76	90.0
77	72.0
78	60.5
79	49.5
80	34.0
81	19.5
82	13.0
83	9.0
84	11.5
85	11.0
86	8.0
87	4.5
88	2.0
89	1.0
90	3.0
91	3.5
92	1.5
93	1.5
94	1.5
95	2.0
96	2.5
97	2.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15047184657945742
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03395970115462984
16-17	0.06888573526894427
18-19	0.026505132608491584
20-21	0.0398957464784066
22-23	0.06460626073319824
24-25	0.01615156518329956
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0209832299817225
38-39	0.00552190262676908
40-41	0.004555569667084491
42-43	0.003037046444722994
44-45	0.01007747229385357
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015875470051961105
60-61	0.016289612748968785
62-63	0.02926608392187612
64-65	0.04417522101415264
66-67	0.01615156518329956
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.015323279789284196
80-81	0.05466683600501388
82-83	0.04721226745887563
84-85	0.028023655830853082
86-87	0.057427787318398434
88-89	0.03161289253825298
90-91	0.0
92-93	0.002484856182046086
94-95	0.0
96-97	0.0
98-99	2.7609513133845397E-4
100-101	0.04169036483210655
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	362194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.68967155085428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20883517365476	52.79024218716442
2	10.351036280120333	12.978061292234406
3	2.356640620442772	4.432110793768764
4	0.8854108087403569	2.2202445115003697
5	0.43087048547460194	1.3505564607679965
6	0.2722073367092107	1.023875311921993
7	0.20164857090347088	0.8848897875046416
8	0.13899921103233775	0.6971051908356108
9	0.1110218344419511	0.6263930102525311
>10	0.873079249690296	10.741864490733628
>50	0.11290759507219744	4.9613860418171045
>100	0.05467572517271275	5.9662589954639484
>500	0.002667108545010378	1.3270119260345314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	988	0.2727819897623925	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	922	0.25455971109405456	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	762	0.21038449007990193	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	713	0.1968558286443177	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	697	0.19243830654290242	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	680	0.1877446893101487	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	499	0.13777147053788855	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	447	0.12341452370828893	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	421	0.11623605029348913	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	407	0.11237071845475077	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	401	0.11071414766672004	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	394	0.10878148174735087	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	370	0.10215519859522798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.7609513133845397E-4	0.0
18-19	0.0	0.0	0.0	4.1414269700768095E-4	0.0
20-21	0.0	0.0	0.0	0.0016565707880307238	0.0
22-23	0.0	0.0	0.0	0.0026229037477153126	0.0
24-25	0.0	0.0	0.0	0.0040033794044075826	0.0
26-27	0.0	0.0	0.0	0.004279474535746036	0.0
28-29	0.0	0.0	0.0	0.005659950192438307	0.0
30-31	0.0	0.0	0.0	0.012562328475899656	0.0
32-33	0.0	0.0	0.0	0.0260909899114839	0.0
34-35	0.0	0.0	0.0	0.04624593449919104	0.0
36-37	0.0	0.0	0.0	0.06929987796595195	0.0
38-39	0.0	0.0	0.0	0.11168048062640464	0.0
40-41	0.0	0.0	0.0	0.1637244128837032	0.0
42-43	0.0	0.0	0.0	0.21424982191864028	0.0
44-45	0.0	0.0	0.0	0.26712203956995423	0.0
46-47	0.0	0.0	0.0	0.3213747328779605	0.0
48-49	0.0	0.0	0.0	0.3819776142067511	0.0
50-51	0.0	0.0	0.0	0.44879263599065694	0.0
52-53	0.0	0.0	0.0	0.5122945161985013	0.0
54-55	0.0	0.0	0.0	0.5807661087704379	0.0
56-57	0.0	0.0	0.0	0.6602815065959127	0.0
58-59	0.0	0.0	0.0	0.7509787572405948	0.0
60-61	0.0	0.0	0.0	0.8587939060282611	0.0
62-63	0.0	0.0	0.0	0.961501294886166	0.0
64-65	0.0	0.0	0.0	1.0597911616426556	0.0
66-67	0.0	0.0	0.0	1.1861046842299983	0.0
68-69	0.0	0.0	0.0	1.3187683948381255	0.0
70-71	0.0	0.0	0.0	1.4594388642550677	0.0
72-73	0.0	0.0	0.0	1.6107389962285406	0.0
74-75	0.0	0.0	0.0	1.764247889252721	0.0
76-77	0.0	0.0	0.0	1.9259015886513857	0.0
78-79	0.0	0.0	0.0	2.1176496573659422	0.0
80-81	0.0	0.0	0.0	2.3269297669204905	0.0
82-83	0.0	0.0	0.0	2.5576072491537687	0.0
84-85	0.0	0.0	0.0	2.781106257972247	0.0
86-87	0.0	0.0	0.0	3.034423540975278	0.0
88-89	0.0	0.0	0.0	3.3125893857987707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	720	0.0	65.3561	1
AAACCGT	135	0.0	63.32312	6
AACCGTG	145	0.0	58.95601	7
CCGTGCG	155	0.0	55.16762	9
ACCGTGC	180	0.0	50.130802	8
GTATCAA	1510	0.0	45.643036	1
TGCGCGG	115	0.0	37.183315	12-13
GACTATC	65	1.3276479E-4	36.53257	7
ATCAACG	1900	0.0	36.49411	3
TCAACGC	1965	0.0	35.286926	4
TATCAAC	2125	0.0	33.077015	2
CAACGCA	2175	0.0	32.09827	5
GTGCGCG	135	0.0	31.67468	10-11
GTAATAC	60	0.00395053	31.661558	3
TGGTATC	345	0.0	31.661558	2
AACGCAG	2230	0.0	31.306608	6
ACGCAGA	2325	0.0	29.823147	7
CGCAGAG	2350	0.0	29.505878	8
AAGACCG	245	0.0	29.07694	5
GTACATG	2770	0.0	28.656303	1
>>END_MODULE