FastQCFastQC Report
Fri 10 Feb 2017
SRR3224523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362194
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.27692341673246934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.2644991358222389No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC7040.1943709724622716No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA5480.15130013197347278No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT5330.14715870500339598No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG5210.1438455634273345No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT5020.13859975593190388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.13363004356781172No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA4260.1176165259501814No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG4070.11237071845475077No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA3730.10298348398924333No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA3670.1013269132012126No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT3640.10049862780719723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.067.886811
CCGTGCG1300.054.798699
AACCGTG1250.053.1912657
ACCGTGC1300.051.1454438
AAACCGT1400.047.49226
GTATCAA16400.045.6605221
CCGGCCA500.001615657637.993769
ATCAACG20950.034.9107363
TGGTATC3150.034.6768462
AGAGCGT550.002579379134.5397838
TCAACGC21450.034.0969664
TATCAAC22650.032.500182
CAACGCA22950.031.8684025
AACGCAG23450.031.3914326
GTCTAAT3500.031.343311
CTAATGT3650.031.2277473
GTACATG30100.030.5827031
TCTAATG3650.029.926592
TACATGG30750.029.6536662
ACGCAGA25200.029.2114737
Produced by FastQC (version 0.11.5)