##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	362194
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.16507451807595	34.0	33.0	34.0	31.0	34.0
2	33.27971749946161	34.0	33.0	34.0	31.0	34.0
3	33.392968961385336	34.0	34.0	34.0	31.0	34.0
4	36.60752248794845	37.0	37.0	37.0	35.0	37.0
5	36.65509091812675	37.0	37.0	37.0	35.0	37.0
6	36.703805143100105	37.0	37.0	37.0	36.0	37.0
7	36.68186938491527	37.0	37.0	37.0	36.0	37.0
8	36.68046406069676	37.0	37.0	37.0	36.0	37.0
9	38.531726091542104	39.0	39.0	39.0	37.0	39.0
10-11	38.557786710989134	39.0	39.0	39.0	37.5	39.0
12-13	38.5501982363043	39.0	39.0	39.0	37.5	39.0
14-15	40.12548247624201	41.0	40.0	41.0	38.0	41.0
16-17	40.132498053529325	41.0	40.0	41.0	38.0	41.0
18-19	40.17013258088207	41.0	40.0	41.0	38.5	41.0
20-21	40.18220346002418	41.0	40.0	41.0	39.0	41.0
22-23	40.091789206889125	41.0	40.0	41.0	38.0	41.0
24-25	40.05050608237575	41.0	40.0	41.0	38.0	41.0
26-27	39.987011104546184	41.0	40.0	41.0	38.0	41.0
28-29	39.94207248049388	41.0	40.0	41.0	38.0	41.0
30-31	39.82831714495546	41.0	40.0	41.0	37.5	41.0
32-33	39.71271335251274	41.0	40.0	41.0	37.0	41.0
34-35	39.59853697189904	41.0	40.0	41.0	36.0	41.0
36-37	39.463094363793985	41.0	40.0	41.0	36.0	41.0
38-39	39.33484955576293	41.0	39.5	41.0	35.0	41.0
40-41	39.176823746390056	41.0	39.0	41.0	35.0	41.0
42-43	39.03472724561975	41.0	39.0	41.0	35.0	41.0
44-45	38.73114408300524	41.0	39.0	41.0	35.0	41.0
46-47	38.40556718222831	40.5	38.0	41.0	35.0	41.0
48-49	38.20817158760222	40.0	38.0	41.0	35.0	41.0
50-51	38.02490930274936	40.0	37.5	41.0	34.5	41.0
52-53	37.78160875111128	40.0	37.0	41.0	34.0	41.0
54-55	37.582468235255135	40.0	36.5	41.0	34.0	41.0
56-57	37.364014864961874	40.0	35.5	41.0	34.0	41.0
58-59	37.10640292219087	39.0	35.0	41.0	33.0	41.0
60-61	36.85768262312462	39.0	35.0	41.0	33.0	41.0
62-63	36.63565105992921	39.0	35.0	41.0	33.0	41.0
64-65	36.37776302202687	38.0	35.0	41.0	33.0	41.0
66-67	36.10206408720188	37.0	35.0	40.0	32.0	41.0
68-69	35.814916867755954	37.0	35.0	40.0	32.0	41.0
70-71	35.51128952992043	36.0	35.0	39.0	32.0	41.0
72-73	35.189228700641095	36.0	35.0	39.0	31.0	41.0
74-75	34.8639472216547	35.0	35.0	38.5	31.0	40.5
76-77	33.02442475579386	34.5	32.0	36.5	28.0	39.0
78-79	34.29248827976167	35.0	34.5	37.0	31.0	39.0
80-81	34.20850152128418	35.0	35.0	36.5	31.0	39.0
82-83	33.99853807627956	35.0	35.0	36.0	31.0	38.0
84-85	33.79421525480819	35.0	35.0	36.0	31.0	37.0
86-87	33.60365577563405	35.0	35.0	35.5	31.0	37.0
88-89	33.45089648089146	35.0	34.5	35.0	31.0	36.0
90-91	33.307006742243104	35.0	34.0	35.0	30.5	36.0
92-93	33.21623770686428	35.0	34.0	35.0	30.0	36.0
94-95	33.108582693252785	35.0	34.0	35.0	30.0	36.0
96-97	33.051632550511606	35.0	34.0	35.0	30.0	35.5
98-99	33.00192162211412	35.0	34.0	35.0	30.0	35.0
100-101	32.190892173807406	34.5	32.5	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	4.0
15	24.0
16	85.0
17	245.0
18	532.0
19	900.0
20	1292.0
21	2053.0
22	2824.0
23	2677.0
24	1802.0
25	1352.0
26	1387.0
27	1676.0
28	2035.0
29	2346.0
30	2532.0
31	2826.0
32	3442.0
33	4593.0
34	7831.0
35	15939.0
36	43870.0
37	95764.0
38	124545.0
39	39567.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15538229836663	18.8770826225998	12.56715565133701	22.400379427696556
2	16.276912372927217	20.457544851654085	39.445159224062245	23.820383551356457
3	19.752950076478353	28.21609413739598	28.675516435943173	23.3554393501825
4	12.549904194989425	16.76366808947691	39.05862604018841	31.627801675345257
5	12.627763022026869	39.201091127959046	34.15848964919353	14.012656200820556
6	28.697327951318908	36.89072706891886	19.725892753607184	14.686052226155045
7	26.635173415351993	32.392585189152776	22.381375726820433	18.590865668674798
8	26.77349707615256	33.3103254057218	20.59421194166662	19.321965576459025
9	28.043810775440786	14.472906784761758	20.647774397146282	36.83550804265118
10-11	25.87632594686825	25.738002286067683	27.492310750592225	20.893361016471836
12-13	26.7414700409173	24.074667167319173	27.601506375036582	21.58235641672695
14-15	24.054374175165798	25.2498660938613	25.916773883609334	24.77898584736357
16-17	22.265001628961276	27.938480482835164	27.000723369244106	22.795794518959454
18-19	22.08912903029868	26.6248198479268	29.23502321960055	22.051027902173974
20-21	24.186962127717145	25.08952458785558	28.906571394650886	21.81694188977639
22-23	23.83690350796059	26.224321179048044	28.02019931500136	21.918575997990008
24-25	22.873684922562113	25.70048882710485	28.89836510042284	22.5274611499102
26-27	22.565691203715208	26.670200752649876	28.753102679002833	22.011005364632084
28-29	22.41491179873158	26.501105794584507	29.086401073490475	21.99758133319344
30-31	22.600199065963725	25.686687843309407	29.293343438484893	22.41976965224198
32-33	22.501846393837532	26.056723979651704	29.26013100768238	22.181298618828386
34-35	22.970665260373597	26.043157919440947	28.740148240592077	22.24602857959338
36-37	22.528279590377345	25.847973560619238	28.878314240986008	22.745432608017406
38-39	22.50189125164967	26.288674025522234	28.453121807650046	22.756312915178054
40-41	22.798969612969845	26.02514122265968	28.965830466545555	22.210058697824923
42-43	22.761282627542144	26.123983279678846	28.75392745324329	22.360806639535717
44-45	22.84546313016683	26.148092311260584	28.3936095602569	22.61283499831569
46-47	22.851012860559155	26.007230869762836	27.93362078135944	23.208135488318568
48-49	22.423082816686755	26.30832032226046	28.13594465910989	23.132652201942893
50-51	22.9279060393049	26.4575061983357	27.47381237679255	23.14077538556685
52-53	23.15527037996212	26.922450399509657	26.92465916056036	22.997620059967865
54-55	23.041243090719338	26.360872902367237	27.29255592306885	23.30532808384457
56-57	23.27749260687926	26.703593153321314	26.79181358567046	23.227100654128964
58-59	23.417738999781804	26.650205352136798	27.070438406787805	22.861617241293594
60-61	23.371587725269855	26.861294113260804	26.9275753362046	22.839542825264743
62-63	23.01377162515116	26.541577165828258	27.45186281385114	22.992788395169438
64-65	23.372833343456822	26.15324936360072	27.09445766633351	23.379459626608945
66-67	22.801454469151892	26.39690331700691	27.020740266266145	23.780901947575057
68-69	23.141189528263858	26.49118980435899	27.33024290849655	23.037377758880602
70-71	23.54760156159406	26.158081028399145	27.251141653368084	23.043175756638707
72-73	23.0176369569899	26.358388046185194	27.3129869627879	23.31098803403701
74-75	23.003832200422977	26.313522587342696	27.2429968469936	23.43964836524073
76-77	23.002653281537743	26.014169241260877	27.011703704930795	23.971473772270585
78-79	23.078293043226154	25.78990239255588	26.998467549321443	24.133337014896526
80-81	22.5833929276502	26.740821324733343	26.96447258339293	23.71131316422353
82-83	22.85179842210979	26.563636740131287	27.36459203324199	23.219972804516935
84-85	22.675472443064553	26.321798300047078	27.410601996485234	23.592127260403135
86-87	22.28350000276095	27.064777439714625	27.17411111172466	23.477611445799766
88-89	23.018603289949585	26.168020453127326	26.86612699271661	23.947249264206473
90-91	22.791791139555045	26.296128594068374	26.953096959088224	23.95898330728836
92-93	22.736296018156015	26.586994814933433	27.223394092668567	23.45331507424198
94-95	22.731050210660587	26.28977840604759	27.13380122254924	23.845370160742586
96-97	22.945714175276233	26.6517391232323	27.03192211908535	23.370624582406112
98-99	21.6713970965836	27.018255410084098	27.41693677973683	23.893410713595475
100-101	22.424393956765762	26.53715826538125	26.986242898011785	24.052204879841195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	6.0
3	9.5
4	9.5
5	11.0
6	14.0
7	28.5
8	54.5
9	89.0
10	104.0
11	109.0
12	136.0
13	157.0
14	185.0
15	224.5
16	240.5
17	258.0
18	285.5
19	318.0
20	368.5
21	422.0
22	493.0
23	616.5
24	764.0
25	1007.5
26	1389.5
27	1864.0
28	2289.0
29	2722.0
30	3425.5
31	4201.0
32	5196.0
33	6375.0
34	7489.0
35	8657.0
36	9921.0
37	10861.5
38	10998.5
39	11018.0
40	10803.0
41	10532.5
42	10444.0
43	10470.5
44	10884.0
45	11678.0
46	12377.0
47	12461.0
48	12563.5
49	13535.5
50	15026.5
51	14684.0
52	13350.0
53	13779.0
54	14383.0
55	13850.5
56	12103.5
57	10237.0
58	9460.5
59	8904.0
60	7321.5
61	5402.0
62	4359.5
63	3138.0
64	1913.0
65	1290.5
66	1086.0
67	969.5
68	764.0
69	530.5
70	352.0
71	256.0
72	218.0
73	201.5
74	152.0
75	113.0
76	86.5
77	58.0
78	40.5
79	31.0
80	19.5
81	13.0
82	14.0
83	8.0
84	3.5
85	3.0
86	2.0
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4558330618397875
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.28285394015362E-4
22-23	9.66332959684589E-4
24-25	1.3804756566922698E-4
26-27	0.001932665919369178
28-29	0.003037046444722994
30-31	6.90237828346135E-4
32-33	4.14142697007681E-4
34-35	0.0034511891417306744
36-37	0.001932665919369178
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007730663677476712
46-47	0.07551201842106717
48-49	6.90237828346135E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.009111139334168981
58-59	0.036720652468014384
60-61	0.027747560699514628
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.7609513133845397E-4
78-79	0.008006758808815165
80-81	0.006350188020784441
82-83	4.14142697007681E-4
84-85	0.0020707134850384045
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06502040343020592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	362194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.167921994645816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29728089098583	50.95121579905923
2	10.860464612759042	13.286241045177128
3	2.4948230728892513	4.578094293387968
4	0.9776248878646923	2.3919713152372744
5	0.4707992799658975	1.4398906816044716
6	0.3156093586373967	1.1583101177947486
7	0.20742579059949007	0.8881463205347141
8	0.17048571753692354	0.8342605657199808
9	0.1258439311515651	0.6927850587760664
>10	0.8993720462369239	10.984434000537348
>50	0.1136881237553413	4.949300403413067
>100	0.06341170249299766	6.524077804497416
>500	0.0027176443925570424	1.04338699727099
>1k	4.529407320928404E-4	0.27788559698959187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1003	0.27692341673246934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	958	0.2644991358222389	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	704	0.1943709724622716	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	548	0.15130013197347278	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	533	0.14715870500339598	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	521	0.1438455634273345	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	502	0.13859975593190388	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	484	0.13363004356781172	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	426	0.1176165259501814	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	407	0.11237071845475077	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	373	0.10298348398924333	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	367	0.1013269132012126	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	364	0.10049862780719723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.7609513133845397E-4	0.0
14-15	0.0	0.0	0.0	2.7609513133845397E-4	0.0
16-17	0.0	0.0	0.0	5.521902626769079E-4	0.0
18-19	0.0	0.0	0.0	6.90237828346135E-4	0.0
20-21	0.0	0.0	0.0	0.002070713485038405	0.0
22-23	0.0	0.0	0.0	0.003313141576061448	0.0
24-25	0.0	0.0	0.0	0.004831664798422945	0.0
26-27	0.0	0.0	0.0	0.005107759929761398	0.0
28-29	0.0	0.0	0.0	0.006488235586453669	0.0
30-31	0.0	0.0	0.0	0.013528661435584243	0.0
32-33	0.0	0.0	0.0	0.027195370436837715	0.0
34-35	0.0	0.0	0.0	0.047212267458875634	0.0
36-37	0.0	0.0	0.0	0.07095644875398267	0.0
38-39	0.0	0.0	0.0	0.11361314654577381	0.0
40-41	0.0	0.0	0.0	0.1657951263687416	0.0
42-43	0.0	0.0	0.0	0.21701077323202483	0.0
44-45	0.0	0.0	0.0	0.2701590860146772	0.0
46-47	0.0	0.0	0.0	0.3251020171510296	0.0
48-49	0.0	0.0	0.0	0.38667123143950477	0.0
50-51	0.0	0.0	0.0	0.45279601539506453	0.0
52-53	0.0	0.0	0.0	0.5165739907342475	0.0
54-55	0.0	0.0	0.0	0.5868402016598839	0.0
56-57	0.0	0.0	0.0	0.6691165507987432	0.0
58-59	0.0	0.0	0.0	0.7618845149284638	0.0
60-61	0.0	0.0	0.0	0.8695616161504608	0.0
62-63	0.0	0.0	0.0	0.9730972904023811	0.0
64-65	0.0	0.0	0.0	1.0737339657752476	0.0
66-67	0.0	0.0	0.0	1.2030845348073131	0.0
68-69	0.0	0.0	0.0	1.3367145783751249	0.0
70-71	0.0	0.0	0.0	1.479593808842775	0.0
72-73	0.0	0.0	0.0	1.6337929396953015	0.0
74-75	0.0	0.0	0.0	1.7900627840328664	0.0
76-77	0.0	0.0	0.0	1.9544774347449159	0.0
78-79	0.0	0.0	0.0	2.1499527877325413	0.0
80-81	0.0	0.0	0.0	2.3628221339944893	0.0
82-83	0.0	0.0	0.0	2.5958464248441446	0.0
84-85	0.0	0.0	0.0	2.821968337410338	0.0
86-87	0.0	0.0	0.0	3.0780465717267544	0.0
88-89	0.0	0.0	0.0	3.3598016532576462	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	815	0.0	67.88681	1
CCGTGCG	130	0.0	54.79869	9
AACCGTG	125	0.0	53.191265	7
ACCGTGC	130	0.0	51.145443	8
AAACCGT	140	0.0	47.4922	6
GTATCAA	1640	0.0	45.660522	1
CCGGCCA	50	0.0016156576	37.99376	9
ATCAACG	2095	0.0	34.910736	3
TGGTATC	315	0.0	34.676846	2
AGAGCGT	55	0.0025793791	34.539783	8
TCAACGC	2145	0.0	34.096966	4
TATCAAC	2265	0.0	32.50018	2
CAACGCA	2295	0.0	31.868402	5
AACGCAG	2345	0.0	31.391432	6
GTCTAAT	350	0.0	31.34331	1
CTAATGT	365	0.0	31.227747	3
GTACATG	3010	0.0	30.582703	1
TCTAATG	365	0.0	29.92659	2
TACATGG	3075	0.0	29.653666	2
ACGCAGA	2520	0.0	29.211473	7
>>END_MODULE