FastQCFastQC Report
Fri 10 Feb 2017
SRR3224520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094292
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA20860.19062553687681166No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC20740.189528937431691No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA20530.1876098884027298No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG18930.17298856246778738No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA17270.15781893681028464No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG12850.11742752391500624No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA12740.11642230775697894No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC12460.11386357571836403No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA12190.1113962269668425No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11860.10838057849276062No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11820.10801504534438705No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT11520.10527354673158534No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA11030.10079576566400925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.051.24821
AAACCGT2900.044.2098856
CCGTGCG3050.042.0586939
GTATCAA17350.041.6575241
AACCGTG3550.037.4527177
ACCGTGC3750.035.455248
GTCTAAT7550.032.7495841
ATCAACG22700.031.1683653
CTAATGT8050.030.6733423
TCAACGC24100.029.3577564
TCTAATG8950.028.1194272
CGCAGAG26950.026.429338
GTACATG39350.026.1010741
GTTATTA9100.025.5686828
ACGCAGA28000.025.268647
TACATGG40050.025.135472
ATGTTAT9300.025.0188166
TATCAAC29050.025.0091512
ACATGGG40550.024.4742323
GTATTAC2653.092282E-1123.3263531
Produced by FastQC (version 0.11.5)