##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224520_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1094292
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29440222536581	34.0	31.0	34.0	31.0	34.0
2	32.4543650140913	34.0	31.0	34.0	31.0	34.0
3	32.59467034393014	34.0	31.0	34.0	31.0	34.0
4	35.87548935750239	37.0	37.0	37.0	35.0	37.0
5	35.89164318116188	37.0	37.0	37.0	35.0	37.0
6	35.990913759764304	37.0	37.0	37.0	35.0	37.0
7	35.94368596316157	37.0	37.0	37.0	35.0	37.0
8	35.979198422358934	37.0	37.0	37.0	35.0	37.0
9	37.718116371133114	39.0	39.0	39.0	35.0	39.0
10-11	37.78448485413399	39.0	39.0	39.0	36.0	39.0
12-13	37.78845545795821	39.0	39.0	39.0	35.0	39.0
14-15	39.303402108395204	41.0	40.0	41.0	37.0	41.0
16-17	39.137880474315814	41.0	40.0	41.0	36.5	41.0
18-19	39.22593375442752	41.0	40.0	41.0	36.5	41.0
20-21	39.28681604178775	41.0	40.0	41.0	37.0	41.0
22-23	39.24375532307647	41.0	40.0	41.0	36.5	41.0
24-25	39.18717307629042	41.0	40.0	41.0	36.0	41.0
26-27	39.02760277878299	41.0	39.5	41.0	35.5	41.0
28-29	38.95262644705435	41.0	39.0	41.0	35.0	41.0
30-31	38.862279903353034	41.0	39.0	41.0	35.0	41.0
32-33	38.669472590496866	41.0	39.0	41.0	35.0	41.0
34-35	38.66646973568298	41.0	39.0	41.0	35.0	41.0
36-37	38.66147563904333	41.0	39.0	41.0	35.0	41.0
38-39	38.546694118206105	41.0	39.0	41.0	35.0	41.0
40-41	38.45446462187424	40.0	38.0	41.0	35.0	41.0
42-43	38.3073982995398	40.0	38.0	41.0	34.0	41.0
44-45	38.13248794654443	40.0	38.0	41.0	33.5	41.0
46-47	37.95694704886813	40.0	38.0	41.0	33.0	41.0
48-49	37.74954216973167	40.0	37.0	41.0	33.0	41.0
50-51	36.48248228078063	38.5	35.5	40.0	31.0	40.5
52-53	36.80867309639474	39.0	36.0	40.0	31.5	40.5
54-55	37.25367589272334	40.0	36.0	41.0	32.0	41.0
56-57	37.102999930548705	39.5	35.0	41.0	32.0	41.0
58-59	36.95979318134465	39.0	35.0	41.0	32.0	41.0
60-61	36.71428832523678	39.0	35.0	41.0	32.0	41.0
62-63	36.432836025484974	38.5	35.0	41.0	31.0	41.0
64-65	36.18853240268594	37.5	35.0	41.0	31.0	41.0
66-67	35.95100667829062	37.0	35.0	40.0	31.0	41.0
68-69	35.648544903919614	36.5	35.0	40.0	30.0	41.0
70-71	35.36201352107116	36.0	35.0	39.5	30.0	41.0
72-73	35.047289023405085	35.5	35.0	39.0	30.0	41.0
74-75	34.767259104516896	35.0	35.0	39.0	29.0	41.0
76-77	34.43573150493653	35.0	34.0	37.5	29.0	40.0
78-79	34.20005080910762	35.0	34.0	37.0	29.0	39.0
80-81	33.89780972537494	35.0	34.0	37.0	29.0	39.0
82-83	33.62375079046544	35.0	34.0	36.0	29.0	38.5
84-85	33.387020100667826	35.0	34.0	36.0	29.0	37.0
86-87	33.155645385326764	35.0	34.0	35.5	28.0	37.0
88-89	32.99058340918146	35.0	34.0	35.0	27.5	36.5
90-91	32.80797355733205	35.0	34.0	35.0	27.0	36.0
92-93	32.670482832735686	35.0	33.5	35.0	27.0	36.0
94-95	32.52443314947016	35.0	33.0	35.0	27.0	36.0
96-97	32.39685659769056	35.0	33.0	35.0	26.0	35.5
98-99	32.34076462223977	35.0	33.0	35.0	26.0	35.0
100-101	31.180466913767077	34.5	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	124.0
8	414.0
9	366.0
10	485.0
11	1004.0
12	1754.0
13	1973.0
14	2193.0
15	2157.0
16	2393.0
17	2502.0
18	2984.0
19	3331.0
20	4008.0
21	5078.0
22	6035.0
23	5850.0
24	5543.0
25	5640.0
26	6124.0
27	6422.0
28	7148.0
29	8329.0
30	10042.0
31	12618.0
32	16674.0
33	23433.0
34	37686.0
35	67930.0
36	145321.0
37	264073.0
38	313304.0
39	121296.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76419653994946	20.15177876030872	13.310752002255082	23.773272697486735
2	15.565315290617129	20.279596305190935	39.526287316365284	24.62880108782665
3	18.050898802784275	27.291784894118464	29.749453754903872	24.907862548193393
4	11.871876976163584	17.22712036641043	40.508566269332135	30.392436388093852
5	12.690031545510704	39.638962909351434	35.11667818096084	12.55432736417702
6	28.6139348546823	39.133521948437895	19.436494098467318	12.81604909841249
7	25.75747606671711	32.25875726040216	24.092015659440076	17.891751013440654
8	25.31975012153977	35.392655707982875	21.29002131058255	17.9975728598948
9	26.362798960423728	14.995723262164029	21.062933842155477	37.57854393525677
10-11	25.231930782643026	25.587137619575028	28.915910926882404	20.265020670899542
12-13	25.520199361779124	23.346876336480573	28.907320897895627	22.225603403844676
14-15	22.8614236729112	25.173527790499644	25.90310782262274	26.061940713966415
16-17	22.73923347076169	27.758210837377163	26.905795355357533	22.596760336503618
18-19	22.717386386331945	27.202656610837682	27.60826872865395	22.471688274176422
20-21	24.072106069311452	25.810125986332245	27.05732805491602	23.060439889440286
22-23	23.923050124311803	26.546582408348048	26.438729687890046	23.091637779450103
24-25	23.186361270409677	26.456753581304422	26.950758121027913	23.40612702725799
26-27	23.08853578386756	27.000745687622686	27.25538521710841	22.655333311401343
28-29	23.000396603465987	27.11163930651051	27.17035306846801	22.717611021555488
30-31	23.04668223837879	26.403647289754474	27.280241471197815	23.269429000668925
32-33	23.116773219579418	26.429600143288994	27.412610162552593	23.041016474578996
34-35	23.517032017048468	26.276396062476927	26.961496565816073	23.245075354658535
36-37	23.053566425960277	26.18933040482272	27.669398206607156	23.08770496260985
38-39	23.052290753367384	26.651843687942485	26.82374271987952	23.472122838810616
40-41	23.55937090324706	26.213095809814252	27.31921053557484	22.90832275136385
42-43	23.457687061774767	26.480056111107302	27.16051751987078	22.90173930724715
44-45	23.39304025487626	26.323093683111885	26.836904050346472	23.446962011665377
46-47	23.480250243993375	26.10203675070274	26.7315762154891	23.686136789814785
48-49	23.027263289871442	26.53816348835594	26.905707069045558	23.52886615272706
50-51	23.11549385356011	26.70685703633034	26.577960909885114	23.599688200224435
52-53	23.56455132633703	26.596877250313444	26.121272932635897	23.71729849071363
54-55	23.27102820819306	26.17957546980148	26.60373099684545	23.94566532516001
56-57	23.434147375654764	26.791249501961083	26.247153410607044	23.527449711777113
58-59	23.80671227061123	26.48075343846095	26.56145679148877	23.15107749943905
60-61	23.574847524611307	26.708716026070483	26.362041876921083	23.354394572397133
62-63	23.241442759852486	26.468543661543116	26.856020632531703	23.433992946072692
64-65	23.88833933374413	26.17154287657682	26.141373213361685	23.79874457631736
66-67	23.25062767280767	26.44026574757271	26.013393399815925	24.295713179803695
68-69	23.336778483256754	26.570741630204736	26.877378250046608	23.215101636491905
70-71	23.70706356255917	25.985157526510292	26.737196287645343	23.570582623285194
72-73	23.532658559141435	26.248752618131178	26.79531605823674	23.423272764490648
74-75	23.468918716393798	26.162075570323097	26.815968681119845	23.553037032163264
76-77	23.39672591959002	25.928545580155937	26.302805832446914	24.37192266780713
78-79	23.563529877353826	25.60726719025192	26.64261696354066	24.186585968853596
80-81	23.288866741945956	26.51281652730855	26.309105288903318	23.88921144184218
82-83	23.183393217952585	26.823418086069285	26.56577708293334	23.42741161304479
84-85	23.185787399667454	26.005062217375524	27.03889534336331	23.770255039593707
86-87	23.10388542279557	27.015458212355327	26.456982116970018	23.423674247879084
88-89	23.413689256000414	26.23247684642425	26.22173590914721	24.132097988428125
90-91	23.126368464724223	26.12360320645678	26.585774180931597	24.1642541478874
92-93	23.224331605535774	26.122078401555747	26.88290942587895	23.77068056702953
94-95	23.481346843438498	25.886417884805883	26.877423941690154	23.75481133006547
96-97	23.414865502078055	26.367505199709036	26.814460856882803	23.403168441330102
98-99	22.579177126293192	26.784837981260857	26.797403240648933	23.838581651797014
100-101	23.318562550167943	26.128653003311314	26.498227323175715	24.05455712334503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	2.5
4	4.5
5	6.0
6	9.0
7	15.0
8	21.0
9	35.5
10	45.0
11	48.0
12	64.5
13	75.0
14	80.5
15	90.5
16	98.5
17	112.0
18	129.5
19	155.0
20	202.0
21	255.0
22	327.5
23	443.0
24	611.0
25	874.5
26	1383.5
27	2300.5
28	3936.0
29	6613.5
30	10220.0
31	14624.5
32	19412.0
33	23957.5
34	27721.0
35	30293.5
36	32754.5
37	33530.5
38	32325.5
39	31585.0
40	30264.5
41	28657.5
42	28100.5
43	28770.5
44	30074.5
45	32425.0
46	35588.0
47	36307.5
48	37756.0
49	41928.5
50	45947.5
51	45751.0
52	43637.5
53	44498.0
54	45723.0
55	44514.5
56	37917.0
57	31760.0
58	29284.5
59	26966.0
60	22840.0
61	17770.5
62	14437.5
63	10773.0
64	6922.0
65	4788.5
66	3793.0
67	3101.0
68	2429.5
69	1720.5
70	1231.0
71	936.0
72	714.5
73	613.5
74	517.0
75	381.0
76	277.5
77	207.5
78	150.0
79	105.0
80	65.5
81	50.5
82	46.0
83	30.5
84	22.0
85	23.5
86	20.0
87	14.0
88	10.5
89	12.5
90	9.5
91	5.5
92	4.0
93	3.0
94	4.0
95	4.5
96	6.5
97	4.5
98	3.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1506910404170002
2	0.0
3	2.741498612801702E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03426873266002128
16-17	0.06876592353777602
18-19	0.024353646010388454
20-21	0.03687315634218289
22-23	0.061318185639664735
24-25	0.015763617023609785
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02015001480409251
38-39	0.004112247919202553
40-41	0.005163155720776539
42-43	0.003152723404721957
44-45	0.010417694728646468
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.014758400865582494
60-61	0.016540374963903603
62-63	0.0266382281877232
64-65	0.04368121123064045
66-67	0.0156265420929697
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.015443775518782921
80-81	0.05364198952381997
82-83	0.04770207586274961
84-85	0.027871902563483972
86-87	0.054967047186674126
88-89	0.031664308977859656
90-91	0.0
92-93	0.002558732038614922
94-95	0.0
96-97	0.0
98-99	4.5691643546695034E-4
100-101	0.04258461178551977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1094292.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.5219599332694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18643781080225	38.22230676480135
2	14.62656456581576	15.071785470426752
3	4.987637681898742	7.70918606425355
4	2.2280024480818033	4.591642114451873
5	1.1891733578066535	3.0634271047312915
6	0.6883837021791933	2.128012651343523
7	0.4319017500572905	1.557669726309246
8	0.2967568621613793	1.2231596129761069
9	0.20292489267295055	0.9409579370782878
>10	0.9759593285569695	9.040671486658102
>50	0.09063367737054437	3.266915183565064
>100	0.08559594676365861	8.807224866503704
>500	0.007162839880589022	2.2925907893633846
>1k	0.0028651359522356085	2.0844502275378063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2086	0.19062553687681166	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	2074	0.189528937431691	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2053	0.1876098884027298	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1893	0.17298856246778738	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1727	0.15781893681028464	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1285	0.11742752391500624	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1274	0.11642230775697894	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1246	0.11386357571836403	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1219	0.1113962269668425	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1186	0.10838057849276062	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1182	0.10801504534438705	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1152	0.10527354673158534	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1103	0.10079576566400925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	4.569164354669503E-5
14-15	0.0	0.0	0.0	0.0	9.138328709339007E-5
16-17	0.0	0.0	0.0	0.0	9.138328709339007E-5
18-19	0.0	0.0	0.0	0.0	9.138328709339007E-5
20-21	0.0	0.0	0.0	9.138328709339007E-5	9.138328709339007E-5
22-23	0.0	0.0	0.0	2.2845821773347514E-4	1.8276657418678013E-4
24-25	0.0	0.0	0.0	6.396830096537304E-4	1.8276657418678013E-4
26-27	0.0	0.0	0.0	0.001233674375760766	1.8276657418678013E-4
28-29	0.0	0.0	0.0	0.0021018156031479716	1.8276657418678013E-4
30-31	0.0	0.0	0.0	0.004157939562749248	2.741498612801702E-4
32-33	0.0	0.0	0.0	0.008909870491605531	3.6553314837356026E-4
34-35	0.0	0.0	0.0	0.01498685908331597	4.5691643546695034E-4
36-37	0.0	0.0	0.0	0.02471917915876201	4.5691643546695034E-4
38-39	0.0	0.0	0.0	0.04400105273546732	4.5691643546695034E-4
40-41	0.0	0.0	0.0	0.06940560654742975	4.5691643546695034E-4
42-43	0.0	0.0	0.0	0.09330233612235125	4.5691643546695034E-4
44-45	0.0	0.0	0.0	0.12099147211164844	5.026080790136453E-4
46-47	0.0	0.0	0.0	0.15105657356537378	5.482997225603404E-4
48-49	0.0	0.0	0.0	0.18569083937376862	5.482997225603404E-4
50-51	0.0	0.0	0.0	0.22402612830944574	5.482997225603404E-4
52-53	0.0	0.0	0.0	0.2637321665515237	5.482997225603404E-4
54-55	0.0	0.0	0.0	0.30554002039674966	5.482997225603404E-4
56-57	0.0	0.0	0.0	0.3525110299627522	5.482997225603404E-4
58-59	0.0	0.0	0.0	0.4090315930300139	5.482997225603404E-4
60-61	0.0	0.0	0.0	0.4719946778373597	5.482997225603404E-4
62-63	0.0	0.0	0.0	0.5413545927412428	5.482997225603404E-4
64-65	0.0	0.0	0.0	0.6054599686372559	5.482997225603404E-4
66-67	0.0	0.0	0.0	0.6798002726877287	5.482997225603404E-4
68-69	0.0	0.0	0.0	0.7726000007310663	5.482997225603404E-4
70-71	0.0	0.0	0.0	0.8685067605355792	5.939913661070354E-4
72-73	0.0	0.0	0.0	0.978806388057301	6.396830096537304E-4
74-75	0.0	0.0	0.0	1.0897913902322234	6.396830096537304E-4
76-77	0.0	0.0	0.0	1.204568798821521	6.396830096537304E-4
78-79	0.0	0.0	0.0	1.3433800119163806	6.396830096537304E-4
80-81	0.0	0.0	0.0	1.500650649004105	6.396830096537304E-4
82-83	0.0	0.0	0.0	1.6739133613331725	6.396830096537304E-4
84-85	0.0	0.0	0.0	1.849277889265388	6.396830096537304E-4
86-87	0.0	0.0	0.0	2.0317246219473413	6.853746532004255E-4
88-89	0.0	0.0	0.0	2.2335903031366398	7.310662967471205E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	900	0.0	51.2482	1
AAACCGT	290	0.0	44.209885	6
CCGTGCG	305	0.0	42.058693	9
GTATCAA	1735	0.0	41.657524	1
AACCGTG	355	0.0	37.452717	7
ACCGTGC	375	0.0	35.45524	8
GTCTAAT	755	0.0	32.749584	1
ATCAACG	2270	0.0	31.168365	3
CTAATGT	805	0.0	30.673342	3
TCAACGC	2410	0.0	29.357756	4
TCTAATG	895	0.0	28.119427	2
CGCAGAG	2695	0.0	26.42933	8
GTACATG	3935	0.0	26.101074	1
GTTATTA	910	0.0	25.568682	8
ACGCAGA	2800	0.0	25.26864	7
TACATGG	4005	0.0	25.13547	2
ATGTTAT	930	0.0	25.018816	6
TATCAAC	2905	0.0	25.009151	2
ACATGGG	4055	0.0	24.474232	3
GTATTAC	265	3.092282E-11	23.326353	1
>>END_MODULE