FastQCFastQC Report
Fri 10 Feb 2017
SRR3224520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094292
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT18740.171252280013013No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA15970.14593910948814393No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG15670.14319761087534222No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC14440.13195746656285526No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG13780.1259261696146915No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT13310.12163115512130217No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13310.12163115512130217No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG12890.11779305706337981No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12770.11669645761825911No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11990.10956856122497469No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG11950.10920302807660112No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG11610.10609599631542586No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT11570.1057304631670523No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT11160.10198374839622332No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA10970.10024746594144891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.058.706421
AAACCGT3400.048.8887376
ACCGTGC3400.048.8887378
AACCGTG3700.044.924787
CCGTGCG3550.044.147419
GTATCAA19500.041.5837141
ATCAACG25500.031.4750333
TCAACGC27200.029.5078454
ATAACGC2253.6379788E-1227.439773
TAGCGTG1056.9135494E-527.1382355
GTACATG46150.026.9760151
GTCTAAT7650.026.8111821
TACATGG47400.025.9502222
ACATGGG47350.025.7770253
CAACGCA31500.025.6305565
TATCAAC31950.025.4182072
CGCAGAG32150.025.1123648
AACGCAG32350.025.1039166
TATAACG2850.024.9957432
ACGCAGA32650.024.7277957
Produced by FastQC (version 0.11.5)