FastQCFastQC Report
Fri 10 Feb 2017
SRR3224519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090754
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG17110.15686396749404538No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA17090.15668060809311724No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA15810.14494560643371465No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13180.12083384521166093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.050.3655661
GTATCAA16150.048.3038141
CCGTGCG2150.037.5666439
ATCAACG21300.036.339663
TCAACGC22050.035.103624
ACCGTGC3100.032.1699528
AACCGTG2550.031.6578667
AAACCGT2400.031.6578666
AATGCGG2150.030.9216377
TATCAAC25750.030.4284342
ACGCAGA25850.030.127027
CGCAGAG26200.029.7259248
AAATGCG2300.028.9050086
GTACATG41950.028.1209411
ATGCGGG2450.027.1365558
TGCGCGG1500.026.92399612-13
TACATGG44150.026.5667922
ACATGGG43200.026.1617093
ATAACGC1301.0042091E-525.5698154
CAACGCA30350.025.5036185
Produced by FastQC (version 0.11.5)