##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224519_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090754
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3499203303403	34.0	31.0	34.0	31.0	34.0
2	32.51304235418802	34.0	31.0	34.0	31.0	34.0
3	32.651856422254696	34.0	31.0	34.0	31.0	34.0
4	35.9257797816923	37.0	37.0	37.0	35.0	37.0
5	35.950448038696166	37.0	37.0	37.0	35.0	37.0
6	36.04470027155527	37.0	37.0	37.0	35.0	37.0
7	36.006131538367036	37.0	37.0	37.0	35.0	37.0
8	36.03849355583385	37.0	37.0	37.0	35.0	37.0
9	37.79056139147782	39.0	39.0	39.0	35.0	39.0
10-11	37.86182218905455	39.0	39.0	39.0	37.0	39.0
12-13	37.86948798720885	39.0	39.0	39.0	37.0	39.0
14-15	39.40064166622355	41.0	40.0	41.0	37.0	41.0
16-17	39.24519139971066	41.0	40.0	41.0	36.5	41.0
18-19	39.330757897747795	41.0	40.0	41.0	36.5	41.0
20-21	39.398175940679565	41.0	40.0	41.0	37.0	41.0
22-23	39.35340278376242	41.0	40.0	41.0	37.0	41.0
24-25	39.3065388712762	41.0	40.0	41.0	37.0	41.0
26-27	39.16153321463868	41.0	40.0	41.0	36.0	41.0
28-29	39.09078994897108	41.0	40.0	41.0	36.0	41.0
30-31	39.02055046325752	41.0	39.0	41.0	36.0	41.0
32-33	38.84275235295951	41.0	39.0	41.0	35.0	41.0
34-35	38.851315236982856	41.0	39.0	41.0	35.0	41.0
36-37	38.85298518272681	41.0	39.0	41.0	35.0	41.0
38-39	38.75764883740972	41.0	39.0	41.0	35.0	41.0
40-41	38.68099819024271	41.0	39.0	41.0	35.0	41.0
42-43	38.54812221637509	40.5	38.5	41.0	35.0	41.0
44-45	38.40013238548747	40.0	38.0	41.0	34.5	41.0
46-47	38.257088674439885	40.0	38.0	41.0	34.0	41.0
48-49	38.07976088100525	40.0	38.0	41.0	33.5	41.0
50-51	36.81979758955732	39.0	36.0	40.0	31.5	40.5
52-53	37.151027179364	39.5	36.5	40.5	32.5	40.5
54-55	37.6361319784296	40.0	37.0	41.0	33.0	41.0
56-57	37.50605911140367	40.0	36.0	41.0	33.0	41.0
58-59	37.38148748480408	40.0	35.5	41.0	33.0	41.0
60-61	37.1539127979361	39.0	35.0	41.0	33.0	41.0
62-63	36.87962592848616	39.0	35.0	41.0	32.0	41.0
64-65	36.64234327813604	39.0	35.0	41.0	32.0	41.0
66-67	36.40550985831819	38.0	35.0	41.0	32.0	41.0
68-69	36.110401612095856	37.0	35.0	40.0	31.0	41.0
70-71	35.813839325824155	37.0	35.0	40.0	31.0	41.0
72-73	35.48744217302893	36.0	35.0	39.0	31.0	41.0
74-75	35.188049734403904	36.0	35.0	39.0	30.5	41.0
76-77	34.829610984694995	35.0	35.0	38.5	30.0	40.0
78-79	34.555196222062904	35.0	35.0	37.0	30.0	39.0
80-81	34.22249746505628	35.0	34.0	37.0	30.0	39.0
82-83	33.92892485381672	35.0	34.0	36.5	29.5	39.0
84-85	33.675599631080885	35.0	34.0	36.0	29.0	37.0
86-87	33.42587283658827	35.0	34.0	36.0	29.0	37.0
88-89	33.24365118074286	35.0	34.0	35.0	29.0	36.5
90-91	33.05837017329297	35.0	34.0	35.0	29.0	36.0
92-93	32.90649312310566	35.0	34.0	35.0	28.5	36.0
94-95	32.76513586014812	35.0	34.0	35.0	27.5	36.0
96-97	32.63094061539083	35.0	33.5	35.0	27.0	35.5
98-99	32.57965086536469	35.0	33.0	35.0	27.0	35.0
100-101	31.41853571016013	34.5	31.0	35.0	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	13.0
7	250.0
8	790.0
9	479.0
10	518.0
11	944.0
12	1466.0
13	1718.0
14	1791.0
15	1882.0
16	1955.0
17	2173.0
18	2455.0
19	2748.0
20	3165.0
21	4085.0
22	4661.0
23	4848.0
24	4788.0
25	5178.0
26	5360.0
27	5668.0
28	6311.0
29	7524.0
30	9075.0
31	11568.0
32	15061.0
33	21181.0
34	34300.0
35	61736.0
36	133385.0
37	253638.0
38	348288.0
39	131728.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.530165729013916	19.884359731770402	13.482825207264568	24.10264933195111
2	15.250459773697827	20.66267921089448	39.77890523436082	24.30795578104687
3	18.2914005278922	27.46460472719305	29.551511662126988	24.69248308278776
4	12.099428468747307	17.662461013207377	40.27819288308821	29.9599176349571
5	12.914461005873001	39.36515474616641	35.16759966041839	12.552784587542195
6	28.61791017956386	38.70799465323987	19.859289995727725	12.814805171468544
7	26.050145128965834	32.09385434295909	23.949121433430452	17.906879094644623
8	25.097684720844484	35.49874673849466	21.460017565830608	17.943550974830256
9	26.545673909974198	15.400722802758459	20.68349050289983	37.37011278436751
10-11	24.88122894804878	25.867565005491617	29.1168311094894	20.134374936970207
12-13	25.575656839213973	23.605643435641767	28.825014622912228	21.993685102232032
14-15	22.78900459120316	25.22745073582147	25.97198503961128	26.01155963336409
16-17	22.680062406618294	27.914392285507066	26.823927919530142	22.581617388344497
18-19	22.652073537267274	27.593302872391888	27.417415007783358	22.33720858255748
20-21	23.970993610541573	26.236859833176112	26.996138259570774	22.796008296711538
22-23	23.71196914597316	26.909019518621403	26.437796549838723	22.941214785566714
24-25	23.04746744327216	26.774275187564534	26.881055386325166	23.297201982838143
26-27	22.966315044455488	27.39224426405954	27.007968799564335	22.633471891920635
28-29	22.89155024872703	27.48662851568731	26.98266520223625	22.63915603334941
30-31	23.063999765299968	26.87535411284304	27.06980675752736	22.99083936432963
32-33	23.004224600597382	26.924448592441557	27.110099985881327	22.96122682107973
34-35	23.417149971487614	26.707763620394697	26.923210916485296	22.951875491632393
36-37	22.76327185810828	26.713960239102757	27.440603726661365	23.0821641761276
38-39	22.812652139511304	27.01640149886357	26.872868900781434	23.298077460843704
40-41	23.335594898642146	26.614620102870663	27.208097626273275	22.84168737221392
42-43	23.170556167794672	26.88280337467017	27.15986959001168	22.786770867523476
44-45	23.273342936442514	26.63120668171011	26.812754958422698	23.28269542342468
46-47	23.15256235594827	26.556262915377804	26.747048372043558	23.54412635663037
48-49	22.814218421385572	26.82795570770311	27.040010854876535	23.31781501603478
50-51	22.876010539498363	27.019566281673047	26.7798697048097	23.324553474018888
52-53	23.23993311049054	26.900015952267882	26.381521406293263	23.478529530948318
54-55	23.192076306848293	26.40127838174327	26.772031090420022	23.634614220988418
56-57	23.10224079042607	26.77969862118251	26.712909928778593	23.40515065961283
58-59	23.380300674732403	26.79018192604764	26.883389167512163	22.946128231707792
60-61	23.242298270460655	26.994990144885545	26.62045453190843	23.14225705274538
62-63	22.911640886833005	26.729070784225705	27.058989682411216	23.300298646530074
64-65	23.538036571500903	26.303669762217822	26.64323409805631	23.51505956822496
66-67	23.034114833588863	26.760622143648355	26.390030003154365	23.815233019608417
68-69	23.01687639926143	26.8389572717588	27.174184096505723	22.969982232474052
70-71	23.388866783894443	26.420301919589566	26.891856458926576	23.298974837589412
72-73	23.223109885455383	26.588167451139306	27.028688411869222	23.160034251536093
74-75	23.24937611963834	26.435291550615446	26.9585534410142	23.356778888732016
76-77	23.093795667950793	26.248310801518947	26.613562728167857	24.044330802362403
78-79	23.26749559859155	25.910403829225352	26.93217246185446	23.88992811032864
80-81	22.936401751555913	26.752232444880892	26.612751939815602	23.69861386374759
82-83	22.945540967186258	26.90875369422884	26.858809371371123	23.286895967213777
84-85	23.055911682871226	26.2236412290886	27.172300203405246	23.548146884634924
86-87	22.94783606998	27.01095254934931	26.771022964164676	23.27018841650602
88-89	23.255439642324887	26.41637051473117	26.546325805342196	23.781864037601743
90-91	22.980800437128813	26.419385122584927	26.648309334643745	23.951505105642518
92-93	22.925417579863566	26.356638897535067	27.07561384939024	23.642329673211123
94-95	23.27247940415529	26.058304622307137	27.01498229664984	23.65423367688773
96-97	23.217975822229395	26.54626982802722	26.974505708894945	23.26124864084844
98-99	22.671649802818347	26.66900757326067	26.99786522153885	23.661477402382133
100-101	23.31480847917949	26.234376246850484	26.64267821878895	23.80813705518107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.5
3	4.0
4	4.5
5	6.0
6	10.0
7	17.0
8	30.5
9	39.0
10	45.5
11	56.0
12	46.5
13	52.0
14	61.0
15	60.5
16	77.0
17	91.0
18	106.5
19	125.0
20	133.5
21	165.0
22	219.5
23	267.0
24	345.5
25	470.0
26	665.5
27	919.0
28	1339.5
29	2444.5
30	4406.0
31	7940.0
32	13161.0
33	19981.0
34	27295.0
35	34063.5
36	40572.5
37	44607.0
38	43897.5
39	40838.5
40	37993.0
41	34776.0
42	32309.5
43	31425.5
44	31586.0
45	33120.0
46	35819.5
47	36705.5
48	37800.0
49	41079.0
50	43700.5
51	42903.0
52	41116.0
53	41183.5
54	42118.0
55	41960.0
56	36358.0
57	30319.0
58	27608.5
59	25469.5
60	21180.5
61	16025.0
62	12916.5
63	9384.0
64	5896.0
65	3958.0
66	2947.5
67	2410.5
68	1905.5
69	1296.5
70	816.0
71	535.0
72	378.0
73	324.0
74	253.5
75	178.0
76	122.5
77	70.5
78	40.5
79	29.5
80	24.5
81	17.5
82	16.0
83	16.0
84	12.0
85	8.0
86	8.5
87	9.5
88	8.5
89	7.0
90	3.5
91	3.5
92	5.0
93	5.5
94	4.5
95	4.5
96	4.5
97	4.5
98	4.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1546636546829074
2	0.0
3	9.167970046408265E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03740531778934572
16-17	0.07384799872381857
18-19	0.026266234182959678
20-21	0.04056826745535657
22-23	0.06582602493321134
24-25	0.017969221290960197
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02388256197089353
38-39	0.0055924617283090415
40-41	0.00586750082970129
42-43	0.003667188018563306
44-45	0.012376759562651158
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.5839850232041326E-5
58-59	0.015906428030518338
60-61	0.018656819044440817
62-63	0.030666859805235647
64-65	0.04891112019758809
66-67	0.018519299493744694
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01778586189003203
80-81	0.05844580904585269
82-83	0.04950703825060463
84-85	0.029337504148506447
86-87	0.058904207548173104
88-89	0.03474660647588732
90-91	0.0
92-93	0.003162949666010851
94-95	0.0
96-97	0.0
98-99	7.792774539447026E-4
100-101	0.046481608135289905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1090754.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.43163859903402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31217407430017	40.449334027208124
2	14.730053990038908	16.035619506601137
3	5.029767620491281	8.21335480067114
4	2.244780086950085	4.8874823370870075
5	1.13907075898921	3.1000743946014024
6	0.6457929487383423	2.109094103731797
7	0.40496022885838523	1.5429854176941213
8	0.28739269034283466	1.2514604045396216
9	0.18544841921534283	0.9084835200142527
>10	0.8714525454643148	8.332565625791855
>50	0.0739731487110445	2.824056037392266
>100	0.0685040637587192	7.437775653568206
>500	0.005099557031973405	1.845241001534715
>1k	0.0015298671095920213	1.0624731695644152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1711	0.15686396749404538	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1709	0.15668060809311724	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1581	0.14494560643371465	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1318	0.12083384521166093	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	4.583985023204132E-5	0.0
22-23	0.0	0.0	0.0	2.291992511602066E-4	0.0
24-25	0.0	0.0	0.0	5.042383525524545E-4	0.0
26-27	0.0	0.0	0.0	8.709571544087851E-4	0.0
28-29	0.0	0.0	0.0	0.0012376759562651156	0.0
30-31	0.0	0.0	0.0	0.004263106071579843	0.0
32-33	0.0	0.0	0.0	0.009763888099424802	0.0
34-35	0.0	0.0	0.0	0.020215373952330223	0.0
36-37	0.0	0.0	0.0	0.03850547419491471	0.0
38-39	0.0	0.0	0.0	0.07600247168472452	0.0
40-41	0.0	0.0	0.0	0.11964200910562786	0.0
42-43	0.0	0.0	0.0	0.1592934795563436	0.0
44-45	0.0	0.0	0.0	0.2081129200534676	0.0
46-47	0.0	0.0	0.0	0.2655044125439833	0.0
48-49	0.0	0.0	0.0	0.3290384449655926	0.0
50-51	0.0	0.0	0.0	0.403803240694052	0.0
52-53	0.0	0.0	0.0	0.4752217273555724	0.0
54-55	0.0	0.0	0.0	0.5465026944663967	0.0
56-57	0.0	0.0	0.0	0.6341484881100596	0.0
58-59	0.0	0.0	0.0	0.7378382293349371	0.0
60-61	4.583985023204132E-5	0.0	0.0	0.8516127376108636	0.0
62-63	9.167970046408264E-5	0.0	0.0	0.9707963482141712	0.0
64-65	9.167970046408264E-5	0.0	0.0	1.0918593926769922	4.583985023204132E-5
66-67	9.167970046408264E-5	0.0	0.0	1.2285079862187074	9.167970046408264E-5
68-69	9.167970046408264E-5	0.0	0.0	1.3876181063741229	9.167970046408264E-5
70-71	9.167970046408264E-5	0.0	0.0	1.5649724869218908	9.167970046408264E-5
72-73	9.167970046408264E-5	0.0	0.0	1.7553912247857903	9.167970046408264E-5
74-75	9.167970046408264E-5	0.0	0.0	1.9468642792050268	1.3751955069612397E-4
76-77	9.167970046408264E-5	0.0	0.0	2.135357743359181	1.8335940092816528E-4
78-79	9.167970046408264E-5	0.0	0.0	2.3701952960979287	1.8335940092816528E-4
80-81	9.167970046408264E-5	0.0	0.0	2.626990137097824	1.8335940092816528E-4
82-83	9.167970046408264E-5	0.0	0.0	2.909867852879751	1.8335940092816528E-4
84-85	9.167970046408264E-5	0.0	0.0	3.1945333228207273	1.8335940092816528E-4
86-87	9.167970046408264E-5	0.0	0.0	3.5016144795251725	1.8335940092816528E-4
88-89	9.167970046408264E-5	0.0	0.0	3.832028119997726	1.8335940092816528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	765	0.0	50.365566	1
GTATCAA	1615	0.0	48.303814	1
CCGTGCG	215	0.0	37.566643	9
ATCAACG	2130	0.0	36.33966	3
TCAACGC	2205	0.0	35.10362	4
ACCGTGC	310	0.0	32.169952	8
AACCGTG	255	0.0	31.657866	7
AAACCGT	240	0.0	31.657866	6
AATGCGG	215	0.0	30.921637	7
TATCAAC	2575	0.0	30.428434	2
ACGCAGA	2585	0.0	30.12702	7
CGCAGAG	2620	0.0	29.725924	8
AAATGCG	230	0.0	28.905008	6
GTACATG	4195	0.0	28.120941	1
ATGCGGG	245	0.0	27.136555	8
TGCGCGG	150	0.0	26.923996	12-13
TACATGG	4415	0.0	26.566792	2
ACATGGG	4320	0.0	26.161709	3
ATAACGC	130	1.0042091E-5	25.569815	4
CAACGCA	3035	0.0	25.503618	5
>>END_MODULE