FastQCFastQC Report
Fri 10 Feb 2017
SRR3224519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090754
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT15550.1425619342216485No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG13610.12477607233161649No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG12240.11221595336803715No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA11890.10900716385179426No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG11840.10854876534947386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17600.045.287191
GGTATCA10000.041.52321
CCGTGCG1550.039.8297739
ATCAACG21550.036.140383
TCAACGC22200.035.082224
TAACGTA650.00585035529.224215
CAACGCA26950.028.8988935
TATCAAC27600.028.7344952
GTACATG46150.028.5436131
AAACCGT2201.8189894E-1228.0618846
ACGCAGA28000.027.8151877
AACCGTG2400.027.7021147
TACATGG47000.027.5842342
ACCGTGC2950.027.366748
CGCAGAG28500.027.32728
GCGTAAT700.00819778527.2730391
ACATGGG46700.027.2529853
AACGCAG28900.026.9489696
AATGCCG1257.403338E-626.5940329
CGATTAT2007.203198E-1026.1191392
Produced by FastQC (version 0.11.5)