##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090754
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18391039592795	34.0	33.0	34.0	31.0	34.0
2	33.314791419513476	34.0	34.0	34.0	31.0	34.0
3	33.43232846269645	34.0	34.0	34.0	31.0	34.0
4	36.640258023348984	37.0	37.0	37.0	35.0	37.0
5	36.6761368741256	37.0	37.0	37.0	35.0	37.0
6	36.715233682388515	37.0	37.0	37.0	36.0	37.0
7	36.69108341569226	37.0	37.0	37.0	36.0	37.0
8	36.679965418416984	37.0	37.0	37.0	36.0	37.0
9	38.53520133779019	39.0	39.0	39.0	38.0	39.0
10-11	38.558777231163035	39.0	39.0	39.0	37.5	39.0
12-13	38.55369863415582	39.0	39.0	39.0	37.5	39.0
14-15	40.17913480033078	41.0	40.0	41.0	38.0	41.0
16-17	40.1885998126067	41.0	40.0	41.0	38.0	41.0
18-19	40.18614554702527	41.0	40.0	41.0	38.5	41.0
20-21	40.18878363040612	41.0	40.0	41.0	39.0	41.0
22-23	40.11843321225501	41.0	40.0	41.0	38.0	41.0
24-25	40.086739539804576	41.0	40.0	41.0	38.0	41.0
26-27	40.03515503954145	41.0	40.0	41.0	38.0	41.0
28-29	40.00519778061781	41.0	40.0	41.0	38.0	41.0
30-31	39.94963942373808	41.0	40.0	41.0	38.0	41.0
32-33	39.91320499397665	41.0	40.0	41.0	38.0	41.0
34-35	39.84295267310503	41.0	40.0	41.0	38.0	41.0
36-37	39.746261301815075	41.0	40.0	41.0	37.5	41.0
38-39	39.65926597564621	41.0	40.0	41.0	37.0	41.0
40-41	39.54665121558115	41.0	40.0	41.0	37.0	41.0
42-43	39.46882340106018	41.0	40.0	41.0	36.0	41.0
44-45	39.26154018229592	41.0	39.0	41.0	35.5	41.0
46-47	39.06650720510766	41.0	39.0	41.0	35.0	41.0
48-49	38.99095946473723	41.0	39.0	41.0	35.0	41.0
50-51	38.862020217207544	40.5	38.5	41.0	35.0	41.0
52-53	38.64903772986393	40.0	38.0	41.0	35.0	41.0
54-55	38.4818643800527	40.0	37.0	41.0	35.0	41.0
56-57	38.282983605835966	40.0	37.0	41.0	35.0	41.0
58-59	38.04910089717755	40.0	36.0	41.0	35.0	41.0
60-61	37.819588559840255	39.5	35.5	41.0	34.0	41.0
62-63	37.60250615629189	39.0	35.0	41.0	34.0	41.0
64-65	37.35033426418789	39.0	35.0	41.0	34.0	41.0
66-67	37.07636689849407	38.5	35.0	41.0	34.0	41.0
68-69	36.809267717560516	37.5	35.0	40.0	33.0	41.0
70-71	36.51717527508494	37.0	35.0	40.0	33.0	41.0
72-73	36.20199146645348	36.5	35.0	39.0	33.0	41.0
74-75	35.87136787946686	36.0	35.0	39.0	33.0	41.0
76-77	33.98073717813549	34.5	33.0	36.5	29.5	39.0
78-79	35.2497231273046	35.0	35.0	37.0	32.5	39.0
80-81	35.13602746357107	35.0	35.0	37.0	33.0	39.0
82-83	34.88517805114627	35.0	35.0	37.0	33.0	39.0
84-85	34.64422775437908	35.0	35.0	36.0	33.0	37.0
86-87	34.419214598342066	35.0	35.0	36.0	33.0	37.0
88-89	34.23986664270771	35.0	35.0	35.5	32.5	37.0
90-91	34.074501216589624	35.0	35.0	35.0	32.0	36.0
92-93	33.97104663379643	35.0	35.0	35.0	32.0	36.0
94-95	33.859580620378196	35.0	35.0	35.0	32.0	36.0
96-97	33.80070024955214	35.0	34.0	35.0	32.0	36.0
98-99	33.75623742842107	35.0	34.0	35.0	32.0	35.5
100-101	32.94545424541189	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	7.0
14	16.0
15	48.0
16	130.0
17	282.0
18	491.0
19	703.0
20	1053.0
21	1475.0
22	2029.0
23	2076.0
24	1993.0
25	2497.0
26	3222.0
27	4236.0
28	5604.0
29	6535.0
30	7142.0
31	8215.0
32	10053.0
33	13520.0
34	22433.0
35	44853.0
36	121672.0
37	272749.0
38	399080.0
39	158604.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57749092108391	20.163853477072855	13.679913623625264	23.57874197821797
2	15.126050420168067	20.887936234934735	40.14470723921251	23.841306105684694
3	17.940800583816333	27.90803425887047	29.848160080091386	24.303005077221812
4	12.017191777431025	17.835093889181245	40.694052004393285	29.45366232899444
5	12.799036262988722	39.00072793682168	35.75040751626856	12.44982828392103
6	28.40805534520158	38.67810707088858	20.16815890659122	12.745678677318626
7	25.882646316217954	32.01656835546787	24.259915618003696	17.840869710310482
8	25.137565390546357	35.288066786828196	21.457633893618542	18.116733929006905
9	26.45335245160687	15.491394026517435	20.902971705810845	37.15228181606485
10-11	24.850332888992384	25.793533647366868	29.36432046089219	19.991813002748557
12-13	25.573960764755387	23.77149201378129	28.970693666949654	21.683853554513664
14-15	22.733356925576253	25.443592230695465	26.303960379700648	25.519090464027638
16-17	22.45080008874595	28.043124297504296	27.09653139021264	22.40954422353711
18-19	22.402576566301843	27.548328954099645	27.920731897384744	22.12836258221377
20-21	23.683454160812474	26.42951934118273	27.382264833358928	22.504761664645873
22-23	23.563959482311674	26.86357391608491	26.877555333177167	22.694911268426246
24-25	23.21337464433892	26.659717323072506	27.09542649762709	23.03148153496148
26-27	22.88436202408861	27.367443429725054	27.259533355398208	22.48866119078813
28-29	22.827678459280232	27.320206727538277	27.404096960606427	22.448017852575063
30-31	23.045240325044936	26.699039139887866	27.43885696106731	22.81686357399989
32-33	22.9249073460753	26.889326405394442	27.559872656720934	22.625893591809323
34-35	23.333142326690123	26.7363306095786	27.082434647077026	22.84809241665425
36-37	22.675955875341973	26.7059248501918	27.51231287727096	23.105806397195266
38-39	22.75526837398717	26.967125493007586	27.09717314811589	23.18043298488935
40-41	23.04185911763789	26.764834233933595	27.369782737445842	22.82352391098268
42-43	23.026731967061316	26.891535579974953	27.472876560617703	22.60885589234603
44-45	23.24684017255844	26.550481595752974	27.123353611853535	23.079324619835052
46-47	23.00752721371974	26.508573921716426	27.006534412446538	23.477364452117293
48-49	22.70889067768795	26.589841006731223	27.40919450867211	23.292073806908718
50-51	23.04405943044903	26.720277899508048	27.021629074933486	23.214033595109438
52-53	23.174473804359184	26.85367186368329	26.678242756845265	23.293611575112262
54-55	23.156412903367762	26.4299282881383	27.006685283757843	23.4069735247361
56-57	23.249911865817825	26.58291971010387	26.788527932232263	23.378640491846042
58-59	23.44070005585552	26.630655600731167	27.02948602832398	22.899158315089338
60-61	23.28551547679913	26.835928275507886	26.765955398022328	23.112600849670656
62-63	22.957742992462098	26.672833654517884	27.170150189685298	23.199273163334723
64-65	23.272525244005525	26.42076031809189	26.908633844111506	23.398080593791086
66-67	23.083114982846727	26.618284232741757	26.72243237246895	23.576168411942565
68-69	23.236403442022674	26.611683294308342	27.15511471880919	22.996798544859796
70-71	23.445433159080782	26.3541091758545	26.97047180207453	23.229985862990187
72-73	23.14765749197344	26.46435401566256	27.11628836566265	23.271700126701347
74-75	23.216600626722432	26.31950008892931	27.009619034172694	23.454280250175565
76-77	22.97953428417668	26.01143615728979	26.917050729268183	24.09197882926534
78-79	23.123454769590186	25.898714945933843	26.873496602269555	24.10433368220642
80-81	22.675618248206973	26.56378001847467	26.870560405984328	23.890041327334032
82-83	22.723799264259274	26.564961780446716	27.175970387008793	23.53526856828522
84-85	22.962778919728695	26.07400942124787	27.2964979289024	23.66671373012104
86-87	22.595012257575952	26.689381840451652	27.140996044937722	23.574609857034677
88-89	23.016372160908876	26.086358610649146	26.663619844621245	24.233649383820733
90-91	22.910619626423557	26.235429803603743	26.71537303553322	24.138577534439477
92-93	22.857043319136725	26.173420484096543	27.16809985024114	23.80143634652559
94-95	22.88013612601925	25.926606732590486	26.995592040001686	24.19766510138858
96-97	22.873718546986762	26.295617526958416	27.136595419315444	23.694068506739374
98-99	21.96911494250766	26.524037500664676	27.307486380980496	24.199361175847166
100-101	22.601713882299837	26.077736181760887	27.018164987203914	24.302384948735366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	3.0
3	3.0
4	3.0
5	9.0
6	11.5
7	14.5
8	28.5
9	42.0
10	40.0
11	32.5
12	42.5
13	61.0
14	64.5
15	70.0
16	84.5
17	89.5
18	95.0
19	115.0
20	137.5
21	169.5
22	221.5
23	305.5
24	382.5
25	500.5
26	732.5
27	1038.5
28	1494.0
29	2734.5
30	4956.5
31	8810.5
32	14566.5
33	21579.5
34	28924.5
35	35284.5
36	40915.0
37	44161.0
38	42915.0
39	39473.5
40	36827.5
41	34329.5
42	32254.0
43	31657.0
44	32160.5
45	33562.5
46	35063.0
47	36532.5
48	38019.0
49	41034.5
50	44377.5
51	43128.5
52	40550.0
53	40737.0
54	42068.0
55	41313.0
56	35430.0
57	29989.0
58	27893.5
59	26140.5
60	21446.5
61	15768.0
62	12553.5
63	8979.5
64	5456.5
65	3520.0
66	2687.0
67	2161.5
68	1638.5
69	1123.0
70	682.5
71	450.0
72	322.5
73	258.5
74	203.0
75	131.5
76	85.0
77	45.5
78	26.5
79	10.0
80	10.0
81	7.5
82	2.0
83	2.0
84	1.5
85	0.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48333538084664374
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0010543165553369505
22-23	0.0018794338595136942
24-25	3.208789516242892E-4
26-27	0.002842070714386562
28-29	0.003942227119955554
30-31	0.001145996255801033
32-33	1.3751955069612397E-4
34-35	0.00380470756925943
36-37	0.002383672212066149
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009993087350585008
46-47	0.08329100787161908
48-49	0.0014668752074253224
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008021973790607232
58-59	0.04043074790466045
60-61	0.030391820703843393
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.7503910139224793E-4
78-79	0.009122130196176224
80-81	0.006555098583181909
82-83	0.0015127150576573637
84-85	0.0025670316129943137
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.5839850232041326E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.07082256860850385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1090754.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.755839779685296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10319594000407	38.56620492393796
2	15.246176120652038	16.086496493479657
3	5.264436903345159	8.331893694094195
4	2.3577718209833733	4.975449296994228
5	1.2375590068981015	3.2644232342911352
6	0.7356105894671159	2.328465263890021
7	0.4436722461956196	1.638441135449244
8	0.3066386378696896	1.2941583079771428
9	0.21383642301087413	1.015300806428042
>10	0.9282758528968236	8.536257717548459
>50	0.07977556792133833	2.9045564514727
>100	0.07556412408570135	7.972345846110873
>500	0.00626799070046636	2.3006581846323146
>1k	0.0012187759695351256	0.7853486436941066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1555	0.1425619342216485	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1361	0.12477607233161649	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1224	0.11221595336803715	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1189	0.10900716385179426	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1184	0.10854876534947386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	9.167970046408264E-5
14-15	0.0	0.0	0.0	0.0	9.167970046408264E-5
16-17	0.0	0.0	0.0	0.0	9.167970046408264E-5
18-19	0.0	0.0	0.0	0.0	9.167970046408264E-5
20-21	0.0	0.0	0.0	4.583985023204132E-5	9.167970046408264E-5
22-23	0.0	0.0	0.0	2.291992511602066E-4	9.167970046408264E-5
24-25	0.0	0.0	0.0	5.042383525524545E-4	9.167970046408264E-5
26-27	0.0	0.0	0.0	8.709571544087851E-4	9.167970046408264E-5
28-29	0.0	0.0	0.0	0.0013293556567291983	9.167970046408264E-5
30-31	0.0	0.0	0.0	0.004308945921811884	9.167970046408264E-5
32-33	0.0	0.0	0.0	0.00971804824919276	9.167970046408264E-5
34-35	0.0	0.0	0.0	0.020307053652794307	9.167970046408264E-5
36-37	0.0	0.0	0.0	0.03887219299677104	9.167970046408264E-5
38-39	0.0	0.0	0.0	0.07678174913866923	9.167970046408264E-5
40-41	0.0	0.0	0.0	0.12074216551119685	9.167970046408264E-5
42-43	0.0	0.0	0.0	0.16080619461400097	9.167970046408264E-5
44-45	0.0	0.0	0.0	0.20971731481158906	9.167970046408264E-5
46-47	0.0	0.0	0.0	0.26706296745187275	9.167970046408264E-5
48-49	0.0	0.0	0.0	0.3307803592744102	9.167970046408264E-5
50-51	0.0	0.0	0.0	0.4062327527563502	1.8335940092816528E-4
52-53	0.0	0.0	0.0	0.4782471574708871	1.8335940092816528E-4
54-55	0.0	0.0	0.0	0.5508574802384405	1.8335940092816528E-4
56-57	0.0	0.0	0.0	0.6391450317853522	1.8335940092816528E-4
58-59	0.0	0.0	0.0	0.743247331662318	1.8335940092816528E-4
60-61	0.0	0.0	0.0	0.8578011173921892	1.8335940092816528E-4
62-63	0.0	0.0	0.0	0.9777640054494414	1.8335940092816528E-4
64-65	0.0	0.0	0.0	1.0998813664675995	1.8335940092816528E-4
66-67	0.0	0.0	0.0	1.238134354767436	1.8335940092816528E-4
68-69	0.0	0.0	0.0	1.3986196704298126	1.8335940092816528E-4
70-71	0.0	0.0	0.0	1.577349246484542	1.8335940092816528E-4
72-73	0.0	0.0	0.0	1.769234859555867	2.291992511602066E-4
74-75	0.0	0.0	0.0	1.962449828283921	2.7503910139224793E-4
76-77	0.0	0.0	0.0	2.1518600894427156	2.7503910139224793E-4
78-79	0.0	0.0	0.0	2.3878436384372645	2.7503910139224793E-4
80-81	0.0	0.0	0.0	2.646105354644585	2.7503910139224793E-4
82-83	0.0	0.0	0.0	2.9313667426385788	3.6671880185633056E-4
84-85	0.0	0.0	0.0	3.2184158847916216	4.5839850232041324E-4
86-87	0.0	0.0	0.0	3.5271014362541875	4.5839850232041324E-4
88-89	0.0	0.0	0.0	3.8599904286392714	4.5839850232041324E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1760	0.0	45.28719	1
GGTATCA	1000	0.0	41.5232	1
CCGTGCG	155	0.0	39.829773	9
ATCAACG	2155	0.0	36.14038	3
TCAACGC	2220	0.0	35.08222	4
TAACGTA	65	0.005850355	29.22421	5
CAACGCA	2695	0.0	28.898893	5
TATCAAC	2760	0.0	28.734495	2
GTACATG	4615	0.0	28.543613	1
AAACCGT	220	1.8189894E-12	28.061884	6
ACGCAGA	2800	0.0	27.815187	7
AACCGTG	240	0.0	27.702114	7
TACATGG	4700	0.0	27.584234	2
ACCGTGC	295	0.0	27.36674	8
CGCAGAG	2850	0.0	27.3272	8
GCGTAAT	70	0.008197785	27.273039	1
ACATGGG	4670	0.0	27.252985	3
AACGCAG	2890	0.0	26.948969	6
AATGCCG	125	7.403338E-6	26.594032	9
CGATTAT	200	7.203198E-10	26.119139	2
>>END_MODULE