FastQCFastQC Report
Fri 10 Feb 2017
SRR3224517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197434
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA55200.46098574117654917No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG54200.4526345502132059No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA49420.41271585740842504No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA44340.3702918073146412No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC39310.3282853167690244No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG38620.3225229950043176No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA37490.31308614921573963No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA35900.29980775558402384No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA35130.2933773385422495No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA34130.28502614757890626No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA33000.2755893017903283No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC31710.2648162654476155No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA29920.24986763362323103No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC29360.24519096668375878No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG29340.2450239428644919No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG26800.2238119178176No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC26570.221891143896031No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC26190.21871769132996055No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT26050.2175485245950925No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT24220.2022658451321743No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG22350.18664911803072237No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG22170.1851459036573206No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA21790.18197245109125013No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT20580.17186751002560474No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC20400.17036429565220296No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC20180.16852703364026744No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA20110.16794245027283342No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT19530.1630987595140943No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG19420.16218012850812655No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT19190.16025935458655757No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG19190.16025935458655757No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC18960.15833858066498863No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG18950.1582550687553552No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT18870.15758697347828776No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC18560.15499810427965133No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC18540.15483108046038446No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC18100.1511565564365134No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA17900.14948631824384476No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT17880.1493192944245779No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG17710.14789959196080954No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG17500.14614584185850746No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA17090.14272185356353667No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC16750.13988244863599997No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG16190.13520578169652775No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT16110.13453768641946026No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT15920.13295096013642504No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC15710.13119721003412296No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT15590.13019506711852177No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA15270.1275226860102519No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC15250.12735566219098507No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA15250.12735566219098507No Hit
GTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAC14960.12493381681161551No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA14950.12485030490198207No Hit
CCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGACGAACAGTGGATT14930.1246832810827152No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA14910.12451625726344834No Hit
GTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGA14640.12226143570334565No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG14470.12084173323957731No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT14120.11791881640240715No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC14050.11733423303497313No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA13890.1159980424808382No Hit
AAATAAACTTGATGGCGGTGCCAGCGTCGGGCTGCTGGCGGAACTCAGCG13880.11591453057120475No Hit
GAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGATGGCTATCAACTGGC13800.11524643529413729No Hit
CGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTC13730.11466185192670326No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT13550.11315863755330148No Hit
CCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGC13270.11082030408356536No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT13180.11006869689686447No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT13170.10998518498723103No Hit
GCTTTAACGGTTCCCGTGCCAGTTGATAGCCATCACCGGGGCGGGAGGCT12850.10731280387896117No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC12840.10722929196932776No Hit
CCGTAGCCCCTCCGATGATAGTTTTCGACTGACCATCAAAGTGCGGCAAT12810.10697875624042744No Hit
CTGCTGGGCGATCTGTTGCGCGAAGTGGTATGGCCGACAGATGTGTCCAC12790.10681173242116059No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG12700.10606012523445968No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA12420.10372179176472357No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT12320.10288667266838923No Hit
TTTCTGCACTGGCGGCCCCACTGCTGGGCGATCTGTTGCGCGAAGTGGTA12270.10246911312022208No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG12240.10221857739132177No Hit
GCATCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGAT12200.10188452975278804No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA12190.10180101784315462No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA12110.10113292256608715No Hit
GTGGTATGGCCGACAGATGTGTCCACGCTGGATGTTGATGCAATGGTGGT12090.10096589874682027No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC12020.10038131537938626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.056.8742451
GTATCAA19950.046.9475331
ACCGTGC2750.046.6204158
CCGTGCG3150.042.232479
AACCGTG2950.041.850097
GTCTAAT6800.039.153321
AAACCGT3200.038.580556
TCTAATG7200.036.2723162
GTTATTA6900.035.0966878
TGTTATT7550.032.075127
TAAGGGT753.084707E-431.6558385
CTAATGT8550.031.6558383
ATCAACG29300.031.4397563
CCATAAT21900.031.2221973
CATAATA21900.031.0053754
ATAATAA22800.030.1980045
GCCCATA22050.030.1862321
CCCATAA22200.029.9447122
ATGTTAT8300.029.748866
TAATGTT8950.029.1799624
Produced by FastQC (version 0.11.5)