FastQCFastQC Report
Fri 10 Feb 2017
SRR3224517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197434
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG53680.4482919309122674No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT48950.4087907976556537No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA45640.3811483555669874No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG44460.3712939502302423No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA41490.3464909130691128No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG38660.32285704264285126No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA37260.3111653752941707No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA33240.2775935876215307No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA33080.27625739706739577No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT32790.2738355516880262No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA32780.2737520397783928No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC32190.26882483711002025No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG32090.26798971801368593No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT31120.25988906277924295No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG30900.25805180076730744No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC29710.24811388352092892No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG29170.24360424040072354No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA29090.24293614512365608No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC28670.23942864491905191No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG28430.23742435908784953No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT27500.2296577514919403No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA25940.21662989358912474No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC23760.1984242972890364No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA23550.1966705471867343No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC22530.18815233240412416No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT22050.18414376074171937No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG21930.18314161782611818No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC21490.17946709380224715No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA20760.17337072439900655No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT20630.1722850695737719No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG20500.1711994147485373No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT20460.17086536711000355No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC20430.17061483138110325No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG20210.16877756936916774No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG20060.16752489072466625No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA19290.1610944736828919No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG17950.14990387779201192No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC17890.14940280633421132No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC17880.1493192944245779No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT17880.1493192944245779No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC16940.14146917491903518No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT16800.14030000818416713No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA16630.1388803057203988No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG16400.13695953179882983No Hit
CCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGACGAACAGTGGATT16380.13679250797956297No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC16070.13420363878092653No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC15630.1305291147570555No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT15580.13011155520888834No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG15500.12944345993182088No Hit
CCGTAGCCCCTCCGATGATAGTTTTCGACTGACCATCAAAGTGCGGCAAT15340.12810726937768596No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT15180.12677107882355104No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA15120.12627000736575042No Hit
GTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGA14930.1246832810827152No Hit
CTGCTGGGCGATCTGTTGCGCGAAGTGGTATGGCCGACAGATGTGTCCAC14860.12409869771528118No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC14830.12384816198638088No Hit
CCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGC14720.12292953098041312No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA14620.1220944118840788No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG14270.11917149504690865No Hit
GTGGTATGGCCGACAGATGTGTCCACGCTGGATGTTGATGCAATGGTGGT14010.11700018539643939No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA13890.1159980424808382No Hit
GAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGATGGCTATCAACTGGC13750.11482887574597014No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT13730.11466185192670326No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG13510.11282458991476775No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT13310.11115435172209909No Hit
GTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGA13270.11082030408356536No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT13230.11048625644503163No Hit
GCTTTAACGGTTCCCGTGCCAGTTGATAGCCATCACCGGGGCGGGAGGCT13190.11015220880649788No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG13170.10998518498723103No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG13100.109400601619797No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC13040.1088995301619964No Hit
ATGTTAAATAAACTTGATGGCGGTGCCAGCGTCGGGCTGCTGGCGGAACT12980.10839845870419582No Hit
GGATATGTCCGCCGTAGCCCCTCCGATGATAGTTTTCGACTGACCATCAA12930.10798089915602864No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC12860.10739631578859461No Hit
GTATAGTACACACACAGTGTAGTACACACACAGTATAGTACACACACAGT12850.10731280387896117No Hit
CGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGCCAT12630.10547554186702565No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA12610.1053085180477588No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT12570.10497447040922507No Hit
TTTCTGCACTGGCGGCCCCACTGCTGGGCGATCTGTTGCGCGAAGTGGTA12570.10497447040922507No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT12470.10413935131289073No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA12470.10413935131289073No Hit
CGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTC12450.10397232749362387No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT12430.10380530367435699No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC12270.10246911312022208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10450.066.195251
AAACCGT3650.055.9484026
GCGTAAT602.2724635E-855.657271
ACCGTGC4000.054.6147468
CCGTGCG3750.054.4564449
GTATCAA22400.050.261921
CGTATAT707.6559445E-847.706231
AACCGTG4450.046.957487
TATACAG7450.036.9729235
GTATCAC651.294789E-436.6970981
ATACAGG6850.033.2784236
ATCAACG34050.032.9159973
TACAAAA6800.032.824722
TCTAATG8050.032.4473272
GTCTAAT8050.032.0016941
GTATAAG5600.031.5201871
TATATAC2300.030.9724463
CAAAACC6900.030.9724454
CGTTATA1701.03682396E-1030.8687361
TATAAGA5700.030.8275472
Produced by FastQC (version 0.11.5)