FastQCFastQC Report
Fri 10 Feb 2017
SRR3224515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458894
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC19920.4340871748159706No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG15310.3336282453028368No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA14280.31118297471747286No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA13220.28808395838690415No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT12930.28176441618325804No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA10240.22314521436322984No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC9200.20048202852946434No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA8740.1904579271029911No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG8700.18958626610938473No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8170.17803675794410037No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA7880.17171721574045423No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA7860.17128138524365102No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG7610.16583350403361125No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA7480.16300060580439057No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA7260.15820647033955554No Hit
GTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACT7090.15450191111672848No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC6970.15188692813590937No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA6490.141426996212633No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT6330.1379403522382075No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC6020.13118497953775818No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT5480.11941755612407223No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC5400.1176742341368595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.11636674264644996No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC5320.11593091214964676No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC5310.11571299690124517No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG5280.11505925115604038No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT5220.11375175966563084No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA5190.11309801392042607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.10917553944919743No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG4840.10547098022637036No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA4770.10394557348755921No Hit
GCACTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCT4730.10307391249395284No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG4700.10242016674874808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT3000.066.4739156
GGTATCA5400.065.187811
AACCGTG3050.063.827417
CCGTGCG3250.061.400689
ACCGTGC3250.059.90618
GTATCAA10750.046.4632151
TACAAAA5850.038.9590722
ATACAAA5400.037.8794021
CAAAACC5450.037.4623644
ATCAACG14600.034.472773
GTCCTAC702.0338704E-433.9782071
GTCTAAT6700.033.369641
TCAACGC15650.032.46334
TCTAATG7150.031.8756032
GTATAGG902.3858605E-531.7129921
AATGCGG1051.9042036E-631.6542457
CTAATGT7350.031.008243
GTACATG22950.030.4693451
TATCAAC16500.030.2154162
TGGTATC2200.030.2154142
Produced by FastQC (version 0.11.5)