##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224515_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458894
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12729737150627	34.0	31.0	34.0	30.0	34.0
2	32.28324623987239	34.0	31.0	34.0	31.0	34.0
3	32.41881785336047	34.0	31.0	34.0	31.0	34.0
4	35.71969125767607	37.0	35.0	37.0	35.0	37.0
5	35.73989635950786	37.0	37.0	37.0	35.0	37.0
6	35.84186326253993	37.0	37.0	37.0	35.0	37.0
7	35.80087776262056	37.0	37.0	37.0	35.0	37.0
8	35.8400959698754	37.0	37.0	37.0	35.0	37.0
9	37.54974351375263	39.0	38.0	39.0	35.0	39.0
10-11	37.62148993013637	39.0	38.5	39.0	35.0	39.0
12-13	37.597755690856715	39.0	38.5	39.0	35.0	39.0
14-15	39.04735734178263	41.0	40.0	41.0	36.0	41.0
16-17	38.882711475852815	41.0	39.5	41.0	35.5	41.0
18-19	38.9663331836982	41.0	39.5	41.0	35.5	41.0
20-21	39.022611975750394	41.0	40.0	41.0	36.0	41.0
22-23	38.97830653702162	41.0	39.5	41.0	35.0	41.0
24-25	38.896005395581554	41.0	39.0	41.0	35.0	41.0
26-27	38.69861122612194	41.0	39.0	41.0	35.0	41.0
28-29	38.61950254307095	41.0	39.0	41.0	35.0	41.0
30-31	38.50547947892106	40.5	39.0	41.0	35.0	41.0
32-33	38.27871142355315	40.0	38.0	41.0	34.0	41.0
34-35	38.24215614063378	40.0	38.0	41.0	34.0	41.0
36-37	38.19483475486714	40.0	38.0	41.0	34.0	41.0
38-39	38.03445457992477	40.0	38.0	41.0	33.5	41.0
40-41	37.886243882029405	40.0	38.0	41.0	33.0	41.0
42-43	37.672523502159535	40.0	37.0	41.0	33.0	41.0
44-45	37.41452710211944	40.0	37.0	41.0	32.0	41.0
46-47	37.13528396536019	40.0	36.0	41.0	31.0	41.0
48-49	36.82488875426569	39.5	35.5	41.0	30.5	41.0
50-51	35.46621550946406	38.0	34.0	40.0	28.0	40.5
52-53	35.752596460184705	38.5	35.0	40.0	29.0	40.5
54-55	36.11559641224335	38.5	35.0	41.0	30.0	41.0
56-57	35.90082677045244	37.5	35.0	41.0	30.0	41.0
58-59	35.70638535260866	37.0	35.0	40.5	29.5	41.0
60-61	35.41979956155452	36.5	35.0	40.0	29.0	41.0
62-63	35.1201759011885	36.0	35.0	40.0	28.5	41.0
64-65	34.86417015694256	35.0	35.0	40.0	28.5	41.0
66-67	34.65121030128962	35.0	34.5	39.0	28.0	41.0
68-69	34.36361774178786	35.0	34.0	39.0	28.0	41.0
70-71	34.103222094862865	35.0	34.0	38.5	27.0	41.0
72-73	33.82882757238055	35.0	34.0	37.0	26.5	40.0
74-75	33.596779430543876	35.0	34.0	37.0	26.5	39.5
76-77	33.32409118445654	35.0	34.0	36.0	26.0	39.0
78-79	33.15320531538874	35.0	34.0	36.0	26.0	39.0
80-81	32.92159191447262	35.0	33.0	36.0	26.0	37.5
82-83	32.69760990555553	35.0	33.0	35.0	25.0	37.0
84-85	32.51103413860282	35.0	33.0	35.0	25.0	37.0
86-87	32.323496275828404	35.0	33.0	35.0	25.0	36.0
88-89	32.213687692582596	35.0	33.0	35.0	25.0	36.0
90-91	32.04526426582174	35.0	33.0	35.0	24.0	36.0
92-93	31.919726559946305	35.0	33.0	35.0	24.0	35.5
94-95	31.780546269944693	35.0	33.0	35.0	24.0	35.0
96-97	31.65543131965116	35.0	32.5	35.0	23.0	35.0
98-99	31.62023146957685	35.0	32.0	35.0	23.5	35.0
100-101	30.37477718165851	34.0	29.5	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	160.0
8	457.0
9	285.0
10	301.0
11	560.0
12	872.0
13	1125.0
14	1090.0
15	1080.0
16	1185.0
17	1408.0
18	1635.0
19	2008.0
20	2535.0
21	3367.0
22	4159.0
23	3715.0
24	3022.0
25	3085.0
26	3088.0
27	3222.0
28	3403.0
29	3911.0
30	4703.0
31	6127.0
32	8082.0
33	11531.0
34	19952.0
35	36572.0
36	80135.0
37	120467.0
38	96585.0
39	29053.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22173825755261	19.00199017134677	13.06159056588951	23.71468100521111
2	16.5112204561402	19.352181549551748	37.720039922073504	26.416558072234547
3	19.99067322736841	26.64994530327265	28.273849734361313	25.085531734997623
4	12.927386280927621	16.489428931300036	38.55552698444521	32.02765780332713
5	13.260578695733654	39.48362802738759	33.386795207607854	13.8689980692709
6	29.013236172187913	37.52544160525089	18.933784272620695	14.527537949940509
7	26.127166622357233	31.43906871739443	22.51783636308167	19.915928297166666
8	26.976600260626636	33.430814087784974	20.156724646650424	19.43586100493796
9	27.15245786608672	14.728455808966775	20.25195361020192	37.867132714744585
10-11	27.13970982405523	24.401277855016627	26.48661782459566	21.972394496332488
12-13	27.00830692926907	22.44113019564431	27.2456166347784	23.30494624030822
14-15	24.19792595434658	24.269320735878548	24.510536997756788	27.02221631201808
16-17	23.486014050827663	26.69744487781581	26.006570719191714	23.809970352164818
18-19	23.669274244699658	26.04791724336354	26.684372622828263	23.59843588910855
20-21	25.40834311612804	23.925173338042153	26.617485641909383	24.048997903920423
22-23	25.322508896797153	25.43099312678808	25.428158362989322	23.81833961342544
24-25	24.159955537634996	25.072741736865623	26.333489532818238	24.433813192681146
26-27	23.88667099591627	26.23993776340506	26.667160607896378	23.206230632782297
28-29	23.63334451964942	26.609739939942557	26.60854140607635	23.148374134331675
30-31	23.48320091350072	24.94094496768317	27.157469916799958	24.418384202016153
32-33	23.79351222722459	25.111790522430017	27.374077673711138	23.720619576634256
34-35	24.429824752557234	24.84005020767323	26.628044820808288	24.10208021896124
36-37	23.69062305200891	24.800024411189042	27.582899962075473	23.92645257472657
38-39	23.715577386796145	25.385209649282427	26.316621029543004	24.582591934378428
40-41	24.434862957127386	24.96213532779365	27.044419891429182	23.558581823649785
42-43	24.341192712232104	25.315306775104958	26.79903285946062	23.544467653202318
44-45	24.183224473517317	24.89827763938742	26.25058025877907	24.667917628316193
46-47	24.48953353061927	24.79025657341347	25.828513774422852	24.89169612154441
48-49	23.867930284553733	25.257902696483285	26.20648777277541	24.667679246187575
50-51	23.995737577741263	25.643285813281498	25.680331405509772	24.680645203467467
52-53	24.759966353885645	25.76510043713799	24.74558394749114	24.729349261485222
54-55	24.2881798410962	24.9179549089768	25.522233892794414	25.271631357132584
56-57	24.3466076551531	25.687550597251867	25.108004362668023	24.857837384927002
58-59	25.023538437396475	25.520243056160968	25.524711000296406	23.931507506146147
60-61	24.60230108515846	25.801772781462297	25.303763727941032	24.292162405438216
62-63	24.38767869929474	25.343175667796146	25.88735228051743	24.381793352391686
64-65	25.426326506586207	24.77141225151847	24.88913984730295	24.91312139459238
66-67	24.05252134265283	25.315317671520386	24.821445306795866	25.81071567903091
68-69	24.18848361495247	25.549691214093016	26.07889839483628	24.182926776118233
70-71	24.91207119726996	24.778380192375582	25.77065727597223	24.53889133438223
72-73	24.58247438406255	25.091851277201272	26.03095704018793	24.29471729854825
74-75	24.6583088905063	24.988123618962113	25.80933723256351	24.54423025796807
76-77	24.711371253492093	24.370333889743602	25.1229042000985	25.795390656665813
78-79	24.553392884581225	23.9374657409597	25.953395717933265	25.555745656525808
80-81	24.308695974497738	25.341781756812743	25.38353691242974	24.965985356259786
82-83	24.158733377155833	25.73056575718629	25.591249453581398	24.519451412076478
84-85	23.966841266623508	24.75929837410602	26.468023794421086	24.805836564849386
86-87	23.866759552100334	26.21702267298549	25.4773188379727	24.438898936941474
88-89	24.504123313561482	25.09172002014244	24.960597994912074	25.443558671384007
90-91	24.009575196014765	24.968293331357568	25.446181471102257	25.575950001525406
92-93	23.988432693114422	25.263059256297133	26.030363090963966	24.718144959624478
94-95	24.447802760550367	24.96458877213474	25.936272864757438	24.651335602557452
96-97	24.501300954032956	25.824373384703218	25.776323072430667	23.89800258883315
98-99	22.98686511546822	26.44290329434454	25.710814624340124	24.85941696584712
100-101	24.1228691710833	25.30821940207071	25.2604740092831	25.30843741756289
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.5
4	2.5
5	5.5
6	6.5
7	7.0
8	12.5
9	22.5
10	29.0
11	31.5
12	42.5
13	54.0
14	68.0
15	81.5
16	87.0
17	99.5
18	108.0
19	114.5
20	142.5
21	175.5
22	218.5
23	312.5
24	437.0
25	611.0
26	938.5
27	1382.5
28	1842.0
29	2425.5
30	3157.0
31	3965.0
32	4729.5
33	5561.5
34	6488.5
35	7257.5
36	8139.0
37	9008.5
38	9406.0
39	9618.5
40	9848.0
41	9944.0
42	10037.5
43	10543.5
44	11310.0
45	12640.0
46	14596.0
47	15249.5
48	16432.5
49	19152.5
50	22180.0
51	22336.0
52	20917.0
53	22493.0
54	24366.0
55	24070.5
56	20000.5
57	16291.5
58	15320.5
59	14604.5
60	12462.0
61	9625.0
62	8024.5
63	5842.0
64	3458.5
65	2302.5
66	1915.0
67	1705.0
68	1406.0
69	931.0
70	563.0
71	388.5
72	298.0
73	252.5
74	202.0
75	138.5
76	114.0
77	95.5
78	57.0
79	46.0
80	35.0
81	22.5
82	16.5
83	11.0
84	10.0
85	7.5
86	7.5
87	5.5
88	4.0
89	4.0
90	2.0
91	2.5
92	2.5
93	2.0
94	3.0
95	2.0
96	1.5
97	1.5
98	2.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13990158947382184
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03857099896708172
16-17	0.07507180307434833
18-19	0.02244527058536394
20-21	0.039333702336487295
22-23	0.06581040501728068
24-25	0.017215304623725742
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.019176541859340066
38-39	0.004467262592232629
40-41	0.0050120507132366075
42-43	0.0033776863502246704
44-45	0.010242016674874808
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.0895762420079582E-4
58-59	0.015254067388111416
60-61	0.014600321642906642
62-63	0.027784194171202935
64-65	0.04532637166753106
66-67	0.015471982636513008
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016016770757516986
80-81	0.05840128657162657
82-83	0.049139888514558916
84-85	0.028546897540608504
86-87	0.05763858320222099
88-89	0.035084354992656254
90-91	0.0
92-93	0.003159771101823079
94-95	0.0
96-97	0.0
98-99	3.268728726023875E-4
100-101	0.04598011741273584
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	458894.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.48838119861286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82735808385287	37.73722606450802
2	13.246640835361589	12.846163408522484
3	4.034460089894422	5.868733163081719
4	1.633540388900529	3.1683091632135665
5	0.8191762233154064	1.9860264492478716
6	0.4827030264138591	1.4043293010287576
7	0.300123425361337	1.0186749338887762
8	0.20193155433793042	0.7833067346212782
9	0.14987057640502124	0.6540283475264252
>10	0.9813865475905046	9.36876790560389
>50	0.15432747668404065	5.228199970063854
>100	0.15524065701912423	14.639402702517984
>500	0.010501573857176053	3.395064431115983
>1k	0.0027395410062198396	1.9017674250594259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1992	0.4340871748159706	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1531	0.3336282453028368	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1428	0.31118297471747286	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1322	0.28808395838690415	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1293	0.28176441618325804	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1024	0.22314521436322984	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	920	0.20048202852946434	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	874	0.1904579271029911	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	870	0.18958626610938473	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	817	0.17803675794410037	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	788	0.17171721574045423	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	786	0.17128138524365102	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	761	0.16583350403361125	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	748	0.16300060580439057	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	726	0.15820647033955554	No Hit
GTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACT	709	0.15450191111672848	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	697	0.15188692813590937	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	649	0.141426996212633	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	633	0.1379403522382075	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	602	0.13118497953775818	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	548	0.11941755612407223	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	540	0.1176742341368595	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.11636674264644996	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	532	0.11593091214964676	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	531	0.11571299690124517	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	528	0.11505925115604038	No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT	522	0.11375175966563084	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	519	0.11309801392042607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	501	0.10917553944919743	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	484	0.10547098022637036	No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA	477	0.10394557348755921	No Hit
GCACTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCT	473	0.10307391249395284	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	470	0.10242016674874808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	2.1791524840159164E-4
10-11	0.0	0.0	0.0	0.0	2.1791524840159164E-4
12-13	0.0	0.0	0.0	0.0	2.1791524840159164E-4
14-15	0.0	0.0	0.0	0.0	2.1791524840159164E-4
16-17	0.0	0.0	0.0	2.1791524840159164E-4	2.1791524840159164E-4
18-19	0.0	0.0	0.0	5.447881210039791E-4	2.1791524840159164E-4
20-21	0.0	0.0	0.0	8.716609936063666E-4	2.1791524840159164E-4
22-23	0.0	0.0	0.0	9.806186178071624E-4	2.1791524840159164E-4
24-25	0.0	0.0	0.0	0.0021791524840159165	2.1791524840159164E-4
26-27	0.0	0.0	0.0	0.0025060253566183037	2.1791524840159164E-4
28-29	0.0	0.0	0.0	0.004685177840634221	2.1791524840159164E-4
30-31	0.0	0.0	0.0	0.00730016082145332	2.1791524840159164E-4
32-33	0.0	0.0	0.0	0.011985338662087541	2.1791524840159164E-4
34-35	0.0	0.0	0.0	0.019067584235139268	2.1791524840159164E-4
36-37	0.0	0.0	0.0	0.03105292289722681	3.2687287260238745E-4
38-39	0.0	0.0	0.0	0.05164591387117722	4.358304968031833E-4
40-41	0.0	0.0	0.0	0.07735991318256503	4.358304968031833E-4
42-43	0.0	0.0	0.0	0.10329182774235444	4.358304968031833E-4
44-45	0.0	0.0	0.0	0.130749149040955	4.358304968031833E-4
46-47	0.0	0.0	0.0	0.16038562282357144	4.358304968031833E-4
48-49	0.0	0.0	0.0	0.1964505964340349	4.358304968031833E-4
50-51	0.0	0.0	0.0	0.24025156136275477	4.358304968031833E-4
52-53	0.0	0.0	0.0	0.2900451956225185	4.358304968031833E-4
54-55	0.0	0.0	0.0	0.3304684742010137	4.358304968031833E-4
56-57	0.0	0.0	0.0	0.3812427270785846	4.358304968031833E-4
58-59	0.0	0.0	0.0	0.4314721918351515	4.358304968031833E-4
60-61	0.0	0.0	0.0	0.49750051210083374	4.358304968031833E-4
62-63	0.0	0.0	0.0	0.5683229678313511	4.358304968031833E-4
64-65	0.0	0.0	0.0	0.6314094322436118	4.358304968031833E-4
66-67	0.0	0.0	0.0	0.6987452439997037	4.358304968031833E-4
68-69	0.0	0.0	0.0	0.7870009196023482	4.358304968031833E-4
70-71	0.0	0.0	0.0	0.8819030102812414	4.358304968031833E-4
72-73	0.0	0.0	0.0	0.9814902788007688	4.358304968031833E-4
74-75	0.0	0.0	0.0	1.0900120725047615	4.358304968031833E-4
76-77	0.0	0.0	0.0	1.1973353323425453	4.358304968031833E-4
78-79	0.0	0.0	0.0	1.3291740576255084	4.358304968031833E-4
80-81	0.0	0.0	0.0	1.4752862316787756	4.358304968031833E-4
82-83	0.0	0.0	0.0	1.6321852105279215	4.358304968031833E-4
84-85	0.0	0.0	0.0	1.7982366298099344	4.358304968031833E-4
86-87	0.0	0.0	0.0	1.9642880490919472	4.358304968031833E-4
88-89	0.0	0.0	0.0	2.167711933474833	4.358304968031833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	300	0.0	66.473915	6
GGTATCA	540	0.0	65.18781	1
AACCGTG	305	0.0	63.82741	7
CCGTGCG	325	0.0	61.40068	9
ACCGTGC	325	0.0	59.9061	8
GTATCAA	1075	0.0	46.463215	1
TACAAAA	585	0.0	38.959072	2
ATACAAA	540	0.0	37.879402	1
CAAAACC	545	0.0	37.462364	4
ATCAACG	1460	0.0	34.47277	3
GTCCTAC	70	2.0338704E-4	33.978207	1
GTCTAAT	670	0.0	33.36964	1
TCAACGC	1565	0.0	32.4633	4
TCTAATG	715	0.0	31.875603	2
GTATAGG	90	2.3858605E-5	31.712992	1
AATGCGG	105	1.9042036E-6	31.654245	7
CTAATGT	735	0.0	31.00824	3
GTACATG	2295	0.0	30.469345	1
TATCAAC	1650	0.0	30.215416	2
TGGTATC	220	0.0	30.215414	2
>>END_MODULE