##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458894
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.14948768125101	34.0	33.0	34.0	31.0	34.0
2	33.26691349200469	34.0	33.0	34.0	31.0	34.0
3	33.38183109824927	34.0	34.0	34.0	31.0	34.0
4	36.60396518585991	37.0	37.0	37.0	35.0	37.0
5	36.63739338496472	37.0	37.0	37.0	35.0	37.0
6	36.67959485197017	37.0	37.0	37.0	36.0	37.0
7	36.653610637750766	37.0	37.0	37.0	35.0	37.0
8	36.64661773742956	37.0	37.0	37.0	35.0	37.0
9	38.480784233395944	39.0	39.0	39.0	37.0	39.0
10-11	38.507039752099615	39.0	39.0	39.0	37.0	39.0
12-13	38.477397830435784	39.0	39.0	39.0	37.0	39.0
14-15	40.042051105484056	41.0	40.0	41.0	38.0	41.0
16-17	40.048114597270825	41.0	40.0	41.0	38.0	41.0
18-19	40.055703495796415	41.0	40.0	41.0	38.0	41.0
20-21	40.053154976966354	41.0	40.0	41.0	38.0	41.0
22-23	39.960474532244916	41.0	40.0	41.0	38.0	41.0
24-25	39.901954481862916	41.0	40.0	41.0	38.0	41.0
26-27	39.81751014395481	41.0	40.0	41.0	37.0	41.0
28-29	39.777841941712026	41.0	40.0	41.0	37.0	41.0
30-31	39.68038479474563	41.0	40.0	41.0	37.0	41.0
32-33	39.59292995768086	41.0	40.0	41.0	36.0	41.0
34-35	39.48155347422281	41.0	39.5	41.0	36.0	41.0
36-37	39.316186308820775	41.0	39.0	41.0	35.0	41.0
38-39	39.15789485153434	41.0	39.0	41.0	35.0	41.0
40-41	38.980143562565644	41.0	39.0	41.0	35.0	41.0
42-43	38.82193600264985	40.5	38.0	41.0	35.0	41.0
44-45	38.48917614961189	40.0	38.0	41.0	35.0	41.0
46-47	38.15843092304541	40.0	37.0	41.0	34.5	41.0
48-49	37.95132753969327	40.0	37.0	41.0	34.0	41.0
50-51	37.70333780785977	40.0	36.0	41.0	34.0	41.0
52-53	37.383912188871506	39.0	35.5	41.0	33.5	41.0
54-55	37.11862107589117	39.0	35.0	41.0	33.0	41.0
56-57	36.833329701412524	38.5	35.0	41.0	33.0	41.0
58-59	36.520740083766626	37.0	35.0	41.0	33.0	41.0
60-61	36.235465052931616	37.0	35.0	40.0	33.0	41.0
62-63	36.01565285229269	36.0	35.0	40.0	32.5	41.0
64-65	35.73972202730914	36.0	35.0	40.0	32.0	41.0
66-67	35.48164499862713	35.0	35.0	39.0	32.0	41.0
68-69	35.24178786386399	35.0	35.0	39.0	31.0	41.0
70-71	34.98050312272551	35.0	35.0	39.0	31.0	41.0
72-73	34.72518816981699	35.0	35.0	37.5	31.0	40.0
74-75	34.45558560364703	35.0	35.0	37.0	31.0	39.5
76-77	32.69915274551421	34.5	32.0	35.5	27.5	38.0
78-79	34.07599358457509	35.0	34.0	36.0	31.0	39.0
80-81	34.07211796188227	35.0	35.0	36.0	31.0	38.0
82-83	33.90867825685234	35.0	35.0	36.0	31.0	37.0
84-85	33.743778519658136	35.0	35.0	35.0	31.0	37.0
86-87	33.59290489742729	35.0	34.0	35.0	31.0	36.5
88-89	33.47660243977912	35.0	34.0	35.0	31.0	36.0
90-91	33.33992054810044	35.0	34.0	35.0	30.0	36.0
92-93	33.24749288506714	35.0	34.0	35.0	30.0	36.0
94-95	33.154154336295534	35.0	34.0	35.0	30.0	35.5
96-97	33.11449702981517	35.0	34.0	35.0	30.0	35.0
98-99	33.09913618395534	35.0	34.0	35.0	30.0	35.0
100-101	32.22483841584331	34.5	32.5	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	7.0
14	8.0
15	37.0
16	111.0
17	216.0
18	569.0
19	926.0
20	1274.0
21	1861.0
22	2761.0
23	2380.0
24	1675.0
25	1529.0
26	1937.0
27	2674.0
28	3341.0
29	3799.0
30	4021.0
31	4533.0
32	5356.0
33	7095.0
34	12549.0
35	27128.0
36	78125.0
37	142425.0
38	116278.0
39	36249.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72969955916666	18.90417675359299	12.949862321663872	23.416261365576478
2	16.409454035136655	19.302061042419382	38.34785375271849	25.94063116972547
3	19.701717607987902	27.11628393485206	28.33660932590097	24.845389131259072
4	12.915400942265535	16.561994709017767	38.920970856014684	31.60163349270202
5	13.278883576599391	38.72419338670804	34.04533508827747	13.951587948415103
6	29.05921628960065	36.99198507716379	19.3042401949034	14.644558438332163
7	26.075738623734456	31.279772670812868	22.971317995005382	19.673170710447295
8	26.985752701059507	33.131834366978	20.00658104050173	19.875831891460773
9	27.195823000518637	14.749375672813331	20.575775669326685	37.479025657341346
10-11	27.352830936991985	24.123544870928797	26.63610768499915	21.887516507080065
12-13	27.28113682026786	22.529494828871155	27.358169860577824	22.831198490283157
14-15	24.31291322178978	24.50576821662519	24.67290921214921	26.50840934943582
16-17	23.348311374740135	26.708891377965283	26.25791577139819	23.684881475896393
18-19	23.327827341390385	25.956321067610382	27.533591635541104	23.18225995545812
20-21	24.956498526343314	24.074265759409883	27.283078280860984	23.686157433385816
22-23	25.21971735837955	25.180056005317237	26.184556043453156	23.41567059285006
24-25	24.54956721843048	24.676503403851015	26.702797172319414	24.071132205399092
26-27	23.939845298425418	26.10880539396165	26.845375787100643	23.105973520512286
28-29	23.704652062347115	26.18764062695788	27.315162378168704	22.7925449325263
30-31	23.56917545149955	24.553948407834163	27.70624608427992	24.17063005638637
32-33	23.785828068852652	25.03459412106962	27.979071373936844	23.200506436140884
34-35	24.529122183068665	24.799129178407288	26.88127755367996	23.790471084844086
36-37	23.757815070746958	24.764158652746087	27.620078058092478	23.857948218414474
38-39	23.671261772871294	25.184901088268752	26.702244962889033	24.44159217597092
40-41	24.086826151573128	25.132056640531363	27.333218564635843	23.447898643259663
42-43	24.1237627861772	25.303773856271818	27.26337672752313	23.30908663002785
44-45	24.230774679119417	24.880762856199514	26.732330183097258	24.156132281583815
46-47	24.223715588277305	24.63861353210634	26.221331969603956	24.9163389100124
48-49	23.849231513503266	24.759473423856313	26.85398865520856	24.537306407431863
50-51	24.34429301755961	25.125628140703515	26.021150854009857	24.508927987727013
52-53	24.89847862303563	25.597987332572806	25.220557711102902	24.282976333288662
54-55	24.399970363526215	24.73305382070805	25.98998897348843	24.8769868422773
56-57	24.714211150859246	25.355581150303525	25.183089014877037	24.747118683960192
58-59	25.218735319091422	25.168595417867028	25.68481840090775	23.927850862133802
60-61	24.604587999877932	25.499169504012976	25.61709557461167	24.27914692149742
62-63	24.523201436497317	25.030290219527824	26.123679978382807	24.322828365592052
64-65	24.985508635981294	24.83885167380702	25.46198032661137	24.71365936360031
66-67	24.217357385365684	24.950206365740236	25.356618304009203	25.475817944884877
68-69	24.49487245420511	25.151777970511706	26.128909944344446	24.224439630938736
70-71	25.25452501013306	24.73011196485463	25.76390190327178	24.251461121740533
72-73	24.59903594294107	25.004903093089037	26.045230488958232	24.35083047501166
74-75	24.67704960186884	24.663865729340547	26.036840751894776	24.62224391689584
76-77	24.68429459590798	24.02117704998769	25.65543601667491	25.63909233742942
78-79	24.48514605507466	24.087864048048676	25.71937899352319	25.707610903353466
80-81	23.846415355849317	25.181422351233675	25.98719910040665	24.984963192510364
82-83	23.91920442200849	25.39625811611368	26.205499617007693	24.479037844870135
84-85	23.96189492448065	24.60823859364683	26.741543161853954	24.688323320018565
86-87	23.58442254638326	25.93659973763004	26.031610785933136	24.447366930053562
88-89	24.31814318775142	24.662231364977533	25.279040475578242	25.740584971692808
90-91	24.194258369035115	24.852907207328926	25.564727366232727	25.388107057403236
92-93	24.09913836310782	25.252672730521645	26.083365657428516	24.564823248942023
94-95	24.234463732365207	24.810522691514816	25.8905106625931	25.06450291352687
96-97	24.22247839370312	25.699290032120707	25.82829585917445	24.249935715001723
98-99	22.38392744293889	26.348023726612247	26.29964654146709	24.96840228898177
100-101	23.555739209884187	25.395177838204415	25.716270053271874	25.332812898639528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	4.5
4	5.0
5	2.5
6	7.0
7	12.0
8	21.5
9	34.5
10	41.5
11	49.5
12	57.5
13	63.0
14	77.5
15	94.5
16	104.0
17	111.5
18	126.0
19	148.0
20	177.5
21	227.0
22	318.0
23	403.5
24	515.0
25	731.0
26	1081.0
27	1519.0
28	1997.0
29	2677.5
30	3340.0
31	3876.0
32	4686.0
33	5582.5
34	6374.0
35	7189.0
36	8212.0
37	9043.5
38	9314.5
39	9598.0
40	9903.5
41	9969.0
42	9989.5
43	10759.0
44	11747.0
45	12880.0
46	14281.0
47	15190.5
48	16482.0
49	19202.5
50	22862.0
51	22677.0
52	20413.5
53	22126.5
54	24439.5
55	23927.5
56	19811.0
57	16152.5
58	15382.5
59	15085.5
60	12833.0
61	9491.5
62	7757.5
63	5482.5
64	3134.5
65	2034.5
66	1682.5
67	1554.5
68	1213.0
69	755.0
70	459.0
71	333.5
72	257.0
73	215.5
74	175.0
75	131.0
76	95.5
77	61.0
78	39.0
79	28.0
80	22.5
81	17.5
82	8.5
83	3.5
84	2.0
85	1.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.44280378475203425
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.268728726023875E-4
22-23	0.0019612372356143247
24-25	4.358304968031833E-4
26-27	0.0022881101082167124
28-29	0.0025060253566183037
30-31	0.0014164491146103457
32-33	2.1791524840159164E-4
34-35	0.0037045592228270585
36-37	0.0010895762420079582
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008607652311862871
46-47	0.0757255488195531
48-49	0.0010895762420079582
50-51	0.0
52-53	1.0895762420079582E-4
54-55	0.0
56-57	0.0070822455730517285
58-59	0.03867995659128252
60-61	0.028764812789010094
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.1791524840159164E-4
78-79	0.005665796458441383
80-81	0.004358304968031833
82-83	0.0016343643630119375
84-85	0.0010895762420079582
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06602832026568227
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	458894.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.31306459706225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32369000733233	35.34783985595737
2	14.010975359097477	12.977824137474578
3	4.284310185794594	5.952586031656706
4	1.8156893134955152	3.3636054585645363
5	0.9308227260864135	2.1554626520826825
6	0.5417992867547075	1.5055431219667812
7	0.3438394426526272	1.1146980823012305
8	0.22420715322919713	0.8306976296501795
9	0.1447206127063022	0.6032209576314063
>10	1.0257046353463044	9.412868335217334
>50	0.16861884809220856	5.492509774795917
>100	0.16956349149876435	15.270047785613558
>500	0.013225007693496939	4.159467655728535
>1k	0.002833930220035059	1.813628521359205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	2132	0.4645953095921934	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	1421	0.30965756797866173	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1412	0.3076963307430474	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1184	0.2580116541074845	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1083	0.23600221401892377	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1059	0.23077224805728558	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	942	0.20527616399429932	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	897	0.1954699778162277	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	878	0.19132958809659747	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	876	0.19089375759979427	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	856	0.18653545263176247	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	844	0.18392046965094336	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	804	0.17520385971487967	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	796	0.17346053772766695	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.15755272459435077	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	712	0.15515565686193325	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	695	0.1514510976391062	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	689	0.15014360614869665	No Hit
GTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACT	688	0.14992569090029506	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	658	0.1433882334482473	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	653	0.14229865720623935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	643	0.14011950472222345	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	622	0.13554328450579	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	619	0.13488953876058524	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	585	0.12748042031493112	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	567	0.12355794584370247	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	566	0.12334003059530088	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	560	0.12203253910489133	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	551	0.12007130186927699	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	533	0.11614882739804835	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	533	0.11614882739804835	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	511	0.11135469193321335	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC	508	0.11070094618800856	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	506	0.11026511569120537	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	500	0.10895762420079583	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	477	0.10394557348755921	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	477	0.10394557348755921	No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG	465	0.10133059050674012	No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG	465	0.10133059050674012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.1791524840159164E-4	0.0
18-19	0.0	0.0	0.0	4.358304968031833E-4	0.0
20-21	0.0	0.0	0.0	6.537457452047749E-4	0.0
22-23	0.0	0.0	0.0	7.627033694055708E-4	0.0
24-25	0.0	0.0	0.0	0.0019612372356143247	0.0
26-27	0.0	0.0	0.0	0.002288110108216712	0.0
28-29	0.0	0.0	0.0	0.004467262592232629	0.0
30-31	0.0	0.0	0.0	0.0070822455730517285	0.0
32-33	0.0	0.0	0.0	0.011985338662087541	0.0
34-35	0.0	0.0	0.0	0.019394457107741656	0.0
36-37	0.0	0.0	0.0	0.03148875339403	0.0
38-39	0.0	0.0	0.0	0.05175487149537802	0.0
40-41	0.0	0.0	0.0	0.078449489424573	0.0
42-43	0.0	0.0	0.0	0.10492619210536638	0.0
44-45	0.0	0.0	0.0	0.1331462167733725	0.0
46-47	0.0	0.0	0.0	0.16278269055598898	0.0
48-49	0.0	0.0	0.0	0.19982828278425957	0.0
50-51	0.0	0.0	0.0	0.24373820533718027	0.0
52-53	0.0	0.0	0.0	0.29396767009374714	0.0
54-55	0.0	0.0	0.0	0.33471782154484475	0.0
56-57	0.0	0.0	0.0	0.38679956591282516	0.0
58-59	0.0	0.0	0.0	0.43768277641459685	0.0
60-61	0.0	0.0	0.0	0.50382005430448	0.0
62-63	0.0	0.0	0.0	0.5757320862770051	0.0
64-65	0.0	0.0	0.0	0.6394722964344707	0.0
66-67	0.0	0.0	0.0	0.7089872606745784	0.0
68-69	0.0	0.0	0.0	0.7989862582644358	0.0
70-71	0.0	0.0	0.0	0.8950868828095377	0.0
72-73	0.0	0.0	0.0	0.9972891343098842	0.0
74-75	0.0	0.0	0.0	1.1096244448609047	0.0
76-77	0.0	0.0	0.0	1.2191268571827045	0.0
78-79	0.0	0.0	0.0	1.352817862077081	0.0
80-81	0.0	0.0	0.0	1.5019808496079703	0.0
82-83	0.0	0.0	0.0	1.6619306419347386	0.0
84-85	0.0	0.0	0.0	1.8297253832039644	0.0
86-87	0.0	0.0	0.0	2.000788853199214	0.0
88-89	0.0	0.0	0.0	2.2071545934355212	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	315	0.0	64.82172	9
ACCGTGC	320	0.0	62.324944	8
GGTATCA	635	0.0	60.87075	1
AAACCGT	335	0.0	59.53427	6
AACCGTG	350	0.0	56.982803	7
GTATCAA	1185	0.0	45.907524	1
TCCTAAC	45	9.4030134E-4	42.41748	1
GTCTAAT	700	0.0	40.22086	1
CAAAACC	510	0.0	40.036938	4
ATACAAA	515	0.0	37.06382	1
TCTAATG	760	0.0	36.863876	2
CTAATGT	755	0.0	36.479057	3
GTTATTA	795	0.0	35.83824	8
TGCGCGG	300	0.0	34.0314	12-13
TAATGTT	875	0.0	33.646988	4
ATCAACG	1650	0.0	32.232697	3
TGGTATC	310	0.0	32.167713	2
ATGTTAT	860	0.0	32.02522	6
TACAAAA	655	0.0	31.898773	2
CGTTAAT	60	0.0039543365	31.657114	2
>>END_MODULE