FastQCFastQC Report
Fri 10 Feb 2017
SRR3224513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128706
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC4630.359734588908054No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG4630.359734588908054No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA4530.3519649433592839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.2851459916398614No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA3350.2602831258837972No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT3170.24629776389601107No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA3160.24552079934113408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.24241294112162604No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA2520.19579506782900566No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC2490.1934641741643746No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT2340.18180970584121953No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA2330.1810327412863425No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA2240.17404006029244945No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG2010.1561698755302783No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC1900.14762326542663123No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA1890.14684630087175424No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG1870.14529237176200022No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA1850.1437384426522462No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC1800.13985361987786118No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA1790.13907665532298416No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG1790.13907665532298416No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG1740.13519183254859915No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT1550.12042950600593601No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA1530.11887557689618199No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1490.11576771867667396No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC1480.11499075412179696No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG1460.11343682501204295No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT1450.11265986045716593No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG1440.11188289590228895No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT1400.10877503768278092No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG1390.1079980731279039No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG1360.10566717946327289No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG1290.10022842757913383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTC200.00207337771.2196962
ACCGTGA200.00207337771.2196968
GGTATCA2500.066.601091
CCGTGCG701.8189894E-1161.092939
ACCGTGC801.8189894E-1259.3497478
AACCGTG753.6379788E-1156.975767
AAACCGT753.6379788E-1156.975766
TAATAGT250.005019621556.9757584
GTGCTAC405.330149E-447.572211
GTAGAGC405.3815293E-447.4797973
GTATCAA4850.045.120031
CACCGTG500.001613857737.983847
CAAAACC1251.3278623E-1037.9838374
GCCCATA1401.0913936E-1137.3781621
ATACAAA1401.0913936E-1137.3781621
CCCATAA1401.0913936E-1137.3055572
ATCAACG6100.036.5827943
TCAACGC6400.034.1261024
TGGTATC702.0483644E-433.9141432
CGGCTTA658.205461E-932.8961916-17
Produced by FastQC (version 0.11.5)