##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224513_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128706
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.035662673068856	34.0	31.0	34.0	30.0	34.0
2	32.190869112551084	34.0	31.0	34.0	30.0	34.0
3	32.32838406911877	34.0	31.0	34.0	30.0	34.0
4	35.61263655152052	37.0	35.0	37.0	35.0	37.0
5	35.620390657778195	37.0	37.0	37.0	35.0	37.0
6	35.73564557984865	37.0	37.0	37.0	35.0	37.0
7	35.68519727130048	37.0	37.0	37.0	35.0	37.0
8	35.737308283996086	37.0	37.0	37.0	35.0	37.0
9	37.433717153823444	39.0	38.0	39.0	35.0	39.0
10-11	37.50944788898731	39.0	38.5	39.0	35.0	39.0
12-13	37.496060789706775	39.0	39.0	39.0	35.0	39.0
14-15	38.95113669914379	41.0	40.0	41.0	36.0	41.0
16-17	38.78744969154508	41.0	39.0	41.0	35.5	41.0
18-19	38.87953164576632	41.0	39.5	41.0	35.0	41.0
20-21	38.93620732522182	41.0	40.0	41.0	35.5	41.0
22-23	38.88853278013457	41.0	39.5	41.0	35.0	41.0
24-25	38.813664475626624	41.0	39.0	41.0	35.0	41.0
26-27	38.612150948673715	41.0	39.0	41.0	35.0	41.0
28-29	38.5282698553292	41.0	39.0	41.0	35.0	41.0
30-31	38.38030472549842	40.5	38.5	41.0	34.0	41.0
32-33	38.119613693223314	40.0	38.0	41.0	34.0	41.0
34-35	38.082890463537055	40.0	38.0	41.0	34.0	41.0
36-37	38.015978276071046	40.0	38.0	41.0	33.5	41.0
38-39	37.83142588535111	40.0	38.0	41.0	33.0	41.0
40-41	37.67195779528538	40.0	37.0	41.0	33.0	41.0
42-43	37.44593492144888	40.0	37.0	41.0	32.0	41.0
44-45	37.13747610833994	40.0	36.0	41.0	31.0	41.0
46-47	36.802643233415694	40.0	36.0	41.0	30.0	41.0
48-49	36.45024707472845	39.5	35.0	41.0	29.5	41.0
50-51	35.077847963575906	38.0	34.0	40.0	27.0	40.5
52-53	35.33887309060961	38.0	34.5	39.5	27.5	40.5
54-55	35.691020620639286	38.0	35.0	40.5	28.0	41.0
56-57	35.459061737603534	37.0	35.0	41.0	28.0	41.0
58-59	35.252804842043105	37.0	35.0	40.0	28.0	41.0
60-61	34.96479185119575	36.0	35.0	40.0	27.0	41.0
62-63	34.6845873541249	35.5	35.0	40.0	26.5	41.0
64-65	34.41928115239383	35.0	34.0	39.5	26.0	41.0
66-67	34.19544543377931	35.0	34.0	39.0	26.0	41.0
68-69	33.90497335011577	35.0	34.0	39.0	26.0	41.0
70-71	33.63657482945628	35.0	34.0	37.5	25.0	41.0
72-73	33.359633583515915	35.0	34.0	37.0	25.0	40.0
74-75	33.13025033797958	35.0	34.0	36.5	24.5	39.5
76-77	32.85963358351592	35.0	33.0	36.0	24.0	39.0
78-79	32.72478750019424	35.0	33.0	36.0	24.0	39.0
80-81	32.480754587975696	35.0	33.0	35.0	24.0	37.0
82-83	32.274656970149024	35.0	33.0	35.0	23.5	37.0
84-85	32.10245443102886	35.0	33.0	35.0	23.0	36.5
86-87	31.930655913477228	35.0	33.0	35.0	23.0	36.0
88-89	31.807044737619073	35.0	33.0	35.0	23.0	36.0
90-91	31.636027069445092	35.0	33.0	35.0	21.5	36.0
92-93	31.50292527154911	35.0	32.5	35.0	20.0	35.5
94-95	31.382122822556838	35.0	32.0	35.0	20.0	35.0
96-97	31.25217550075366	35.0	32.0	35.0	20.0	35.0
98-99	31.19462962099669	35.0	32.0	35.0	20.0	35.0
100-101	29.963502090034652	34.0	29.5	35.0	16.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	68.0
8	197.0
9	102.0
10	109.0
11	252.0
12	377.0
13	386.0
14	363.0
15	366.0
16	397.0
17	423.0
18	523.0
19	627.0
20	844.0
21	1184.0
22	1495.0
23	1269.0
24	1033.0
25	950.0
26	952.0
27	879.0
28	1020.0
29	1082.0
30	1322.0
31	1694.0
32	2187.0
33	3061.0
34	5418.0
35	10088.0
36	22764.0
37	33782.0
38	25806.0
39	7679.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.086367880485525	18.773731714908184	12.515561780267662	23.624338624338627
2	17.272699019470732	19.06204838935248	36.85919848336519	26.8060541078116
3	21.723151989806226	25.216384628533245	27.954407719919814	25.10605566174071
4	13.481888956225818	15.981383929265148	37.24457290258418	33.29215421192485
5	13.331934797134554	38.81948005531988	33.72181561077184	14.12676953677373
6	30.193619567075352	36.43808369462185	18.00226873650024	15.36602800180256
7	26.621136543750872	31.042841825555918	21.242210930337357	21.09381070035585
8	27.29631874193899	32.71020776032197	19.581060712010316	20.412412785728716
9	27.060898481811257	14.510590026882975	20.254688981088684	38.17382251021708
10-11	27.765993815362144	23.836107096794244	25.864373067300672	22.533526020542944
12-13	27.654887884014734	22.143101331717247	26.55936786163815	23.642642922629868
14-15	24.661138140612128	24.14779351188348	24.210747205944074	26.98032114156032
16-17	23.34359596336552	26.907916219620283	26.0122685077203	23.736219309293897
18-19	23.522623511355466	25.502690731062888	27.295863850950987	23.678821906630663
20-21	24.885460586395165	23.46280140672664	27.71834379311015	23.933394213768043
22-23	24.687490282658043	25.13798625579153	26.556329487857212	23.61819397369321
24-25	23.75142788315836	24.586788098253905	27.306565542751017	24.355218475836722
26-27	23.6298230074744	25.859322797693967	27.208133264960455	23.302720929871178
28-29	23.340015228505276	26.27888365732755	27.532515966621602	22.84858514754557
30-31	23.41887713082529	24.77351483225335	27.70189423958479	24.105713797336563
32-33	23.376921044861934	25.05904930617065	27.911286187124144	23.65274346184327
34-35	24.00354295837024	24.782061442357	27.32040464314018	23.893990956132583
36-37	23.432206024058935	24.621942121786702	28.224177053868395	23.72167480028597
38-39	23.642692587110485	25.029234313508393	26.89362594841552	24.434447150965607
40-41	24.188529271220617	24.892872266446002	27.478312218583312	23.440286243750073
42-43	24.148326567083	24.961830578271595	27.506847186340593	23.38299566830481
44-45	23.87387037368	24.6948163458774	27.183685204323467	24.24762807611914
46-47	24.057153512656754	24.570338601153015	26.804500178701847	24.568007707488384
48-49	23.484142153434963	25.259894643606355	26.722141935884885	24.533821267073797
50-51	23.591363262007985	25.90089040137989	26.00500365173341	24.50274268487872
52-53	24.38697496620204	25.945954345562754	25.03807126318897	24.62899942504623
54-55	24.11542585427253	25.138688173045548	25.63400307677964	25.111882895902287
56-57	24.301120382888133	25.82474787500194	25.130918527496775	24.74321321461315
58-59	24.865862423995182	25.856596149736777	25.791712803776445	23.485828622491596
60-61	24.452085397361927	26.2210307515978	25.2738116051829	24.053072245857372
62-63	24.361689313371592	25.439172517767012	25.83278610812134	24.366352060740056
64-65	25.405634383027675	24.567744342493384	25.31352865964798	24.713092614830963
66-67	23.861530392213997	25.255551022786875	25.15725469627212	25.725663888727002
68-69	24.037729398784826	25.54737152891085	26.228380961260545	24.186518111043775
70-71	24.74360169689059	24.81352850682952	25.87253119512688	24.570338601153015
72-73	24.350069149845385	25.117710130063863	26.234208195422127	24.298012524668625
74-75	24.456513293863534	25.13208397432909	26.062499028794306	24.348903703013068
76-77	24.616567992168196	24.389305859866674	25.405963979923236	25.588162168041894
78-79	24.43526641748712	23.990395449494518	25.982795732346975	25.591542400671386
80-81	23.942616387988462	25.606523479707405	25.660548961078334	24.790311171225795
82-83	23.82344139263186	25.60737202532482	25.938895517631376	24.630291064411942
84-85	23.846844291437254	24.724995045830923	26.78942644321745	24.638734219514376
86-87	23.537436646870432	26.490936227107365	25.65607412704829	24.315552998973914
88-89	24.428364913807844	24.96308271027311	25.280182798874606	25.32836957704444
90-91	23.66905971749569	25.104501732630958	25.800661973800754	25.4257765760726
92-93	23.58958221642075	25.309821837874797	26.485008119468855	24.615587826235597
94-95	24.418053548397122	24.86675057883859	26.025204730160212	24.68999114260407
96-97	24.44291641415319	25.47511382530729	25.94867372150482	24.1332960390347
98-99	22.847119975447825	26.579672197380845	25.90565282757924	24.66755499959209
100-101	23.852365999253824	25.68710359408034	25.403401318244	25.05712908842184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	5.5
2	3.0
3	3.0
4	4.5
5	4.5
6	5.5
7	14.0
8	21.0
9	30.0
10	33.5
11	29.5
12	39.0
13	53.5
14	61.0
15	61.5
16	56.5
17	62.5
18	80.5
19	92.0
20	99.5
21	115.0
22	155.5
23	205.0
24	291.5
25	414.0
26	522.5
27	651.5
28	803.0
29	923.0
30	1083.0
31	1296.5
32	1452.5
33	1567.0
34	1741.0
35	1902.0
36	2033.0
37	2118.0
38	2188.5
39	2332.0
40	2436.5
41	2514.5
42	2527.5
43	2578.5
44	2825.0
45	3332.5
46	3935.5
47	4137.0
48	4409.5
49	5063.0
50	5915.5
51	6090.5
52	5856.0
53	6422.0
54	7066.5
55	7022.5
56	5876.0
57	4853.0
58	4518.5
59	4294.5
60	3678.5
61	2830.0
62	2334.0
63	1642.5
64	982.5
65	667.0
66	536.5
67	487.5
68	419.0
69	278.5
70	161.5
71	112.5
72	84.0
73	63.0
74	55.5
75	45.5
76	29.0
77	18.5
78	12.5
79	7.5
80	4.5
81	2.5
82	3.5
83	3.5
84	1.5
85	2.0
86	2.5
87	2.5
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14451540720712322
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03107858219508026
16-17	0.06526502260966854
18-19	0.018258667039609655
20-21	0.029913135362764753
22-23	0.05438751884139046
24-25	0.014762326542663124
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.017093220207294143
38-39	0.0034963404969465296
40-41	0.0034963404969465296
42-43	0.0027193759420695227
44-45	0.010100539213401085
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.010489021490839589
60-61	0.010489021490839589
62-63	0.020589560704240673
64-65	0.03845974546641182
66-67	0.010489021490839589
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.013208397432909112
80-81	0.048948766957251415
82-83	0.04467546190542788
84-85	0.020589560704240673
86-87	0.048171802402374404
88-89	0.03107858219508026
90-91	0.0
92-93	0.0023308936646310196
94-95	0.0
96-97	0.0
98-99	3.8848227743850327E-4
100-101	0.03884822774385033
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	128706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.69020869267944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.90285771861107	53.61055428651345
2	8.498847592538949	10.485913632620079
3	1.7821383140845601	3.2982145354528924
4	0.625952467915213	1.5446055350954888
5	0.35516820111084524	1.0955200223765793
6	0.24307610927089765	0.8997249545475735
7	0.14357863449161828	0.6200177147918512
8	0.11712993866421491	0.5780616288284929
9	0.11964886207634856	0.6643046944198405
>10	0.9886774392624592	12.953553058909453
>50	0.1536543281401529	6.5389336938448865
>100	0.06927039383367549	7.710596242599413
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	463	0.359734588908054	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	463	0.359734588908054	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	453	0.3519649433592839	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.2851459916398614	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	335	0.2602831258837972	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	317	0.24629776389601107	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	316	0.24552079934113408	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	312	0.24241294112162604	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	252	0.19579506782900566	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	249	0.1934641741643746	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	234	0.18180970584121953	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	233	0.1810327412863425	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	224	0.17404006029244945	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	201	0.1561698755302783	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	190	0.14762326542663123	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	189	0.14684630087175424	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	187	0.14529237176200022	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	185	0.1437384426522462	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	180	0.13985361987786118	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	179	0.13907665532298416	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	179	0.13907665532298416	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	174	0.13519183254859915	No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT	155	0.12042950600593601	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	153	0.11887557689618199	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	149	0.11576771867667396	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	148	0.11499075412179696	No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG	146	0.11343682501204295	No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT	145	0.11265986045716593	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	144	0.11188289590228895	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	140	0.10877503768278092	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	139	0.1079980731279039	No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG	136	0.10566717946327289	No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG	129	0.10022842757913383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.8848227743850327E-4	0.0
22-23	0.0	0.0	0.0	7.769645548770065E-4	0.0
24-25	0.0	0.0	0.0	7.769645548770065E-4	0.0
26-27	0.0	0.0	0.0	0.001553929109754013	0.0
28-29	0.0	0.0	0.0	0.005438751884139045	0.0
30-31	0.0	0.0	0.0	0.013208397432909112	0.0
32-33	0.0	0.0	0.0	0.022143489813994686	0.0
34-35	0.0	0.0	0.0	0.04428697962798937	0.0
36-37	0.0	0.0	0.0	0.07420011499075413	0.0
38-39	0.0	0.0	0.0	0.12159495283825152	0.0
40-41	0.0	0.0	0.0	0.16898979068574893	0.0
42-43	0.0	0.0	0.0	0.2136652525911768	0.0
44-45	0.0	0.0	0.0	0.26494491321305924	0.0
46-47	0.0	0.0	0.0	0.3212748434416422	0.0
48-49	0.0	0.0	0.0	0.38382049010924124	0.0
50-51	0.0	0.0	0.0	0.44481220766708623	0.0
52-53	0.0	0.0	0.0	0.5135735707737014	0.0
54-55	0.0	0.0	0.0	0.5710689478345998	0.0
56-57	0.0	0.0	0.0	0.6534271906515625	0.0
58-59	0.0	0.0	0.0	0.7555980296178888	0.0
60-61	0.0	0.0	0.0	0.8445604711513062	0.0
62-63	0.0	0.0	0.0	0.940904075956055	0.0
64-65	0.0	0.0	0.0	1.0422979503675043	0.0
66-67	0.0	0.0	0.0	1.1518499526051622	0.0
68-69	0.0	0.0	0.0	1.2850993737665688	0.0
70-71	0.0	0.0	0.0	1.435053532857831	0.0
72-73	0.0	0.0	0.0	1.5811228691747083	0.0
74-75	0.0	0.0	0.0	1.7295230991562165	0.0
76-77	0.0	0.0	0.0	1.9210448619333986	0.0
78-79	0.0	0.0	0.0	2.1005236741099873	0.0
80-81	0.0	0.0	0.0	2.306419281152394	0.0
82-83	0.0	0.0	0.0	2.539508647615496	0.0
84-85	0.0	0.0	0.0	2.7555047938713035	0.0
86-87	0.0	0.0	0.0	2.997140770438053	0.0
88-89	0.0	0.0	0.0	3.2698553291998818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTC	20	0.002073377	71.219696	2
ACCGTGA	20	0.002073377	71.219696	8
GGTATCA	250	0.0	66.60109	1
CCGTGCG	70	1.8189894E-11	61.09293	9
ACCGTGC	80	1.8189894E-12	59.349747	8
AACCGTG	75	3.6379788E-11	56.97576	7
AAACCGT	75	3.6379788E-11	56.97576	6
TAATAGT	25	0.0050196215	56.975758	4
GTGCTAC	40	5.330149E-4	47.57221	1
GTAGAGC	40	5.3815293E-4	47.479797	3
GTATCAA	485	0.0	45.12003	1
CACCGTG	50	0.0016138577	37.98384	7
CAAAACC	125	1.3278623E-10	37.983837	4
GCCCATA	140	1.0913936E-11	37.378162	1
ATACAAA	140	1.0913936E-11	37.378162	1
CCCATAA	140	1.0913936E-11	37.305557	2
ATCAACG	610	0.0	36.582794	3
TCAACGC	640	0.0	34.126102	4
TGGTATC	70	2.0483644E-4	33.914143	2
CGGCTTA	65	8.205461E-9	32.89619	16-17
>>END_MODULE