##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128706
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.12124531878856	34.0	33.0	34.0	31.0	34.0
2	33.24505462060821	34.0	33.0	34.0	31.0	34.0
3	33.360503783817386	34.0	34.0	34.0	31.0	34.0
4	36.58004288844343	37.0	37.0	37.0	35.0	37.0
5	36.62239522632977	37.0	37.0	37.0	35.0	37.0
6	36.66724939008282	37.0	37.0	37.0	35.0	37.0
7	36.647786427983156	37.0	37.0	37.0	35.0	37.0
8	36.64030425931969	37.0	37.0	37.0	35.0	37.0
9	38.474624337637714	39.0	39.0	39.0	37.0	39.0
10-11	38.50576896181997	39.0	39.0	39.0	37.5	39.0
12-13	38.48408388109334	39.0	39.0	39.0	37.0	39.0
14-15	40.05531599148446	41.0	40.0	41.0	38.0	41.0
16-17	40.05253057355523	41.0	40.0	41.0	38.0	41.0
18-19	40.06760368591985	41.0	40.0	41.0	38.0	41.0
20-21	40.06916538467515	41.0	40.0	41.0	38.0	41.0
22-23	39.98350115767718	41.0	40.0	41.0	38.0	41.0
24-25	39.93679781828353	41.0	40.0	41.0	38.0	41.0
26-27	39.84199260329744	41.0	40.0	41.0	37.5	41.0
28-29	39.79408496884372	41.0	40.0	41.0	37.0	41.0
30-31	39.67640203253927	41.0	40.0	41.0	37.0	41.0
32-33	39.549578108246706	41.0	40.0	41.0	35.5	41.0
34-35	39.425955277920224	41.0	39.5	41.0	35.0	41.0
36-37	39.26166223796871	41.0	39.0	41.0	35.0	41.0
38-39	39.080730502074495	41.0	39.0	41.0	35.0	41.0
40-41	38.88400696160241	41.0	39.0	41.0	35.0	41.0
42-43	38.69687116373751	40.5	38.0	41.0	35.0	41.0
44-45	38.319693720572474	40.0	37.5	41.0	35.0	41.0
46-47	37.94197240221901	40.0	37.0	41.0	34.0	41.0
48-49	37.68609855018414	40.0	36.5	41.0	34.0	41.0
50-51	37.42417602908955	40.0	36.0	41.0	33.5	41.0
52-53	37.07338430220813	39.0	35.0	41.0	33.0	41.0
54-55	36.8044924090563	39.0	35.0	41.0	33.0	41.0
56-57	36.50543875188414	38.0	35.0	41.0	33.0	41.0
58-59	36.192489083648	37.0	35.0	40.5	32.0	41.0
60-61	35.894056998119744	36.5	35.0	40.0	32.0	41.0
62-63	35.675213276770315	36.0	35.0	40.0	31.5	41.0
64-65	35.40631361397293	35.5	35.0	39.5	31.0	41.0
66-67	35.150952558544276	35.0	35.0	39.0	31.0	41.0
68-69	34.902514257299586	35.0	35.0	39.0	31.0	41.0
70-71	34.636846767050486	35.0	35.0	38.5	30.5	41.0
72-73	34.383144530946495	35.0	35.0	37.0	30.0	40.0
74-75	34.11239957733128	35.0	34.5	37.0	30.0	39.5
76-77	32.36302503379795	34.5	32.0	35.5	27.0	38.0
78-79	33.71594952838252	35.0	34.0	36.0	30.0	39.0
80-81	33.71111680885117	35.0	35.0	36.0	30.0	37.5
82-83	33.580439140366416	35.0	35.0	35.0	30.0	37.0
84-85	33.422894037574004	35.0	34.0	35.0	30.0	37.0
86-87	33.279167249390085	35.0	34.0	35.0	30.0	36.0
88-89	33.15429739095302	35.0	34.0	35.0	29.5	36.0
90-91	33.01864326449427	35.0	34.0	35.0	29.0	36.0
92-93	32.930407284819665	35.0	34.0	35.0	29.0	36.0
94-95	32.83559430018802	35.0	34.0	35.0	29.0	35.5
96-97	32.809569872422415	35.0	34.0	35.0	29.0	35.0
98-99	32.789722312868086	35.0	34.0	35.0	29.0	35.0
100-101	31.926642891551285	34.5	32.5	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	5.0
15	13.0
16	36.0
17	87.0
18	203.0
19	299.0
20	476.0
21	762.0
22	1120.0
23	1034.0
24	698.0
25	531.0
26	617.0
27	791.0
28	916.0
29	1043.0
30	1157.0
31	1309.0
32	1520.0
33	1949.0
34	3333.0
35	7311.0
36	21724.0
37	40434.0
38	31414.0
39	9915.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.302208137873976	18.219283935777455	12.445655143345535	23.032852783003037
2	17.197333457647662	19.2640591736205	37.33547775550479	26.203129613227045
3	21.741022174568396	25.465790250648766	27.804453560828556	24.988734013954282
4	13.711093499914535	16.27896135378304	37.553027830870356	32.45691731543207
5	13.28143210106755	38.376610259039985	33.97432909110687	14.367628548785605
6	30.29540192376424	35.702298261153324	18.686774509346886	15.315525305735553
7	26.5729647413485	31.004770562366947	21.60971516479418	20.812549531490372
8	27.029819899616182	32.66669774524886	19.30601525958386	20.9974670955511
9	27.049244013488106	14.45387161437695	20.347924727673924	38.14895964446102
10-11	27.70616754463661	23.893990956132583	25.98052926825478	22.419312230976026
12-13	27.73297282177987	22.359874442527932	26.571799294516186	23.335353441176014
14-15	24.521778316473203	24.44485882554038	24.64415023386633	26.38921262412009
16-17	23.376921044861934	26.753609000357404	26.147965129830773	23.721504824949886
18-19	23.167529097322582	25.596320295868104	27.865445278386403	23.37070532842292
20-21	24.437866144546486	24.02957127095862	28.087268658803787	23.44529392569111
22-23	24.58334304561665	25.091878200197353	27.074737962596057	23.25004079158994
24-25	24.056858487011045	24.23556102886046	27.715987273271146	23.991593210857346
26-27	23.607636244969	25.835651349629373	27.66934468772824	22.887367717673385
28-29	23.37176956906867	25.904054453060652	28.253352810455485	22.470823167415187
30-31	23.318052911697293	24.54877432889165	28.44644730196962	23.686725457441433
32-33	23.432085528258202	25.116156200954116	28.588799278976897	22.862958991810796
34-35	24.307608632310952	24.480876439851595	27.655251451991997	23.55626347584546
36-37	23.231601193436102	24.454562406762808	28.52436598707111	23.789470412729983
38-39	23.583982098736655	25.011265986045718	27.33633241651516	24.06841949870247
40-41	23.879228629589917	25.022143489814	27.77182104952372	23.326806831072368
42-43	23.776669308346154	25.144515407207123	27.767547744471898	23.311267539974825
44-45	23.819199732700312	24.838668319158938	27.383066098395815	23.95906584974494
46-47	23.87043098957321	24.714060228129785	26.95103840262497	24.464470379672036
48-49	23.41176881927205	24.822752895197915	27.338204957868605	24.427273327661425
50-51	23.981010986278804	25.443258278557334	26.257517132068436	24.318213603095426
52-53	24.420772924339193	26.210899258775815	25.35041101424953	24.017916802635465
54-55	24.113871925162776	25.026028312588377	26.173993442419157	24.68610631982969
56-57	24.64918414918415	25.200854700854702	25.716394716394714	24.433566433566433
58-59	24.991353481341008	25.52763194198866	25.72349016239911	23.757524414271224
60-61	24.567811527357364	25.879239799030902	25.628999856228607	23.923948817383128
62-63	24.542367877177444	25.1402421021553	26.137476108339936	24.17991391232732
64-65	24.790219570183208	24.704753469146738	25.896617096328068	24.608409864341986
66-67	23.916911410501456	24.996892141780492	25.655369602038753	25.4308268456793
68-69	24.15388559973894	25.198125961493638	26.22915792581542	24.418830512952
70-71	24.948720339378117	24.90676425341476	25.788230540922726	24.3562848662844
72-73	24.295681631003994	25.21832703992044	25.954500955666404	24.531490373409166
74-75	24.418830512952	24.786334747408823	26.140972448836884	24.653862290802294
76-77	24.48331857100679	23.997327241931224	26.08192314266623	25.43743104439575
78-79	24.23660859971096	24.13054964180821	25.937436869667913	25.695404888812917
80-81	23.583549595188884	25.262233687122187	26.139842426691118	25.01437429099781
82-83	23.52472708907968	25.2437745231343	26.609300337982205	24.622198049803814
84-85	23.880875331668033	24.745251756917924	26.87530835900564	24.498564552408407
86-87	23.14111230245676	26.149130576663094	26.259848025733067	24.449909095147078
88-89	24.064534675928083	24.441362485043435	25.727627305642315	25.766475533386167
90-91	23.662455518779232	24.972029276024426	25.8970055786055	25.468509626590834
92-93	23.749956295574005	25.391300294082225	26.226540435334933	24.632202975008838
94-95	23.64575078084938	24.929684707783633	26.203518095504485	25.221046415862507
96-97	24.162820692120025	25.598651189532735	25.856991904029336	24.381536214317904
98-99	22.075893897720388	26.506145789629077	26.423787546812115	24.99417276583842
100-101	23.239017791546974	25.603968016419376	25.708143996081738	25.448870195951905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	5.0
2	3.0
3	6.0
4	7.5
5	5.0
6	5.0
7	13.0
8	25.5
9	36.0
10	34.5
11	38.0
12	46.5
13	50.0
14	58.0
15	61.5
16	62.5
17	73.5
18	86.0
19	98.0
20	117.5
21	127.5
22	150.0
23	206.5
24	277.0
25	396.0
26	533.0
27	667.0
28	802.0
29	949.0
30	1115.0
31	1227.0
32	1402.0
33	1651.5
34	1809.0
35	1927.5
36	2037.0
37	2112.5
38	2243.5
39	2393.5
40	2479.0
41	2548.5
42	2640.0
43	2740.0
44	2958.5
45	3419.5
46	3838.0
47	4225.5
48	4511.0
49	5123.5
50	6189.0
51	6268.0
52	5759.5
53	6241.5
54	6904.0
55	6739.0
56	5734.0
57	4863.5
58	4648.0
59	4468.0
60	3722.5
61	2718.5
62	2234.5
63	1571.5
64	866.5
65	567.5
66	446.0
67	397.5
68	348.0
69	236.0
70	123.0
71	81.0
72	54.0
73	51.0
74	49.0
75	32.0
76	21.0
77	11.5
78	4.5
79	1.5
80	0.5
81	1.0
82	2.0
83	1.5
84	1.0
85	1.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.45763212282255683
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0023308936646310196
24-25	3.8848227743850327E-4
26-27	0.003107858219508026
28-29	0.006215716439016052
30-31	7.769645548770065E-4
32-33	0.0
34-35	0.0027193759420695227
36-37	0.001553929109754013
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008935092381085575
46-47	0.07381163271331562
48-49	0.0011654468323155098
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.004661787329262039
58-59	0.032244029027395774
60-61	0.022920454368871693
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.003107858219508026
80-81	0.003107858219508026
82-83	7.769645548770065E-4
84-85	3.8848227743850327E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.8848227743850327E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.060214753002968004
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	128706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.55277920221279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.67085899175451	51.01937749599863
2	9.45882475733222	11.265986045716595
3	1.8004383676025466	3.2166332571908067
4	0.6940820373656195	1.6533805727782698
5	0.41096962738753784	1.2237191739312852
6	0.2596284312702223	0.9276956785231457
7	0.19309049159795427	0.8049352788525788
8	0.13959920676338589	0.6650816589747176
9	0.08871725289635737	0.47550230758472795
>10	1.045037052499739	13.57512470281106
>50	0.15655985805239536	6.532717977405871
>100	0.08219392547750756	8.639845850232312
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	471	0.36595030534707007	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	381	0.2960234954081395	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.27970723975572237	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	347	0.2696067005423213	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	338	0.26261401954842817	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	337	0.2618370549935512	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	332	0.2579522322191662	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	304	0.23619722468260998	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	272	0.21133435892654578	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	266	0.20667257159728372	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	251	0.19501810327412863	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	236	0.18336363495097355	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	235	0.18258667039609652	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	230	0.1787018476217115	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	223	0.17326309573757245	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	215	0.1670473792985564	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	202	0.15694684008515533	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	194	0.15073112364613928	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	184	0.1429614780973692	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	170	0.1320839743290911	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	166	0.1289761161095831	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	165	0.12819915155470607	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	162	0.12586825789007505	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	160	0.12431432878032105	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	160	0.12431432878032105	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	159	0.12353736422544404	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	158	0.12276039967056704	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	155	0.12042950600593601	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	154	0.11965254145105901	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	153	0.11887557689618199	No Hit
CTGCTGGGCGATCTGTTGCGCGAAGTGGTATGGCCGACAGATGTGTCCAC	150	0.11654468323155098	No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG	148	0.11499075412179696	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	148	0.11499075412179696	No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC	143	0.11110593134741192	No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG	142	0.11032896679253493	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	141	0.10955200223765792	No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC	140	0.10877503768278092	No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC	138	0.1072211085730269	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	134	0.10411325035351887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	7.769645548770065E-4	0.0
20-21	0.0	0.0	0.0	0.0011654468323155098	0.0
22-23	0.0	0.0	0.0	0.001553929109754013	0.0
24-25	0.0	0.0	0.0	0.001553929109754013	0.0
26-27	0.0	0.0	0.0	0.0023308936646310196	0.0
28-29	0.0	0.0	0.0	0.006992680993893059	0.0
30-31	0.0	0.0	0.0	0.014762326542663124	0.0
32-33	0.0	0.0	0.0	0.0236974189237487	0.0
34-35	0.0	0.0	0.0	0.04584090873774338	0.0
36-37	0.0	0.0	0.0	0.07614252637794663	0.0
38-39	0.0	0.0	0.0	0.12470281105775954	0.0
40-41	0.0	0.0	0.0	0.17365157801501097	0.0
42-43	0.0	0.0	0.0	0.21949248675275435	0.0
44-45	0.0	0.0	0.0	0.2723260764843908	0.0
46-47	0.0	0.0	0.0	0.3309869003776048	0.0
48-49	0.0	0.0	0.0	0.3927555824903268	0.0
50-51	0.0	0.0	0.0	0.4541357823256103	0.0
52-53	0.0	0.0	0.0	0.5236741099871023	0.0
54-55	0.0	0.0	0.0	0.5811694870480009	0.0
56-57	0.0	0.0	0.0	0.6643046944198405	0.0
58-59	0.0	0.0	0.0	0.7715258029928674	0.0
60-61	0.0	0.0	0.0	0.8624306559134772	0.0
62-63	0.0	0.0	0.0	0.9595512252731031	0.0
64-65	0.0	0.0	0.0	1.0621105465168679	0.0
66-67	0.0	0.0	0.0	1.1743819246965952	0.0
68-69	0.0	0.0	0.0	1.3095737572451944	0.0
70-71	0.0	0.0	0.0	1.4603048808913337	0.0
72-73	0.0	0.0	0.0	1.6083166285954036	0.0
74-75	0.0	0.0	0.0	1.7633210572933664	0.0
76-77	0.0	0.0	0.0	1.9548428200705483	0.0
78-79	0.0	0.0	0.0	2.138206455021522	0.0
80-81	0.0	0.0	0.0	2.346044473451121	0.0
82-83	0.0	0.0	0.0	2.5810762513014156	0.0
84-85	0.0	0.0	0.0	2.799791773499293	0.0
86-87	0.0	0.0	0.0	3.0472549842276195	0.0
88-89	0.0	0.0	0.0	3.3257967771510266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	265	0.0	63.01655	1
TTATACT	25	0.0050153155	56.988113	4
AACCGTG	45	1.5102532E-5	52.76677	7
CCGTGCG	45	1.5102532E-5	52.76677	9
ACCGTGC	55	8.1452527E-7	51.807377	8
AAACCGT	50	2.8163822E-5	47.490093	6
AAGTGAC	45	9.6030487E-4	42.213417	7
GTATCAA	455	0.0	39.847824	1
GTGCGCG	45	5.684378E-7	36.93674	10-11
ACATTGG	60	0.0038528424	31.808352	1
TTGTACA	60	0.0039420645	31.660065	4
ATCAACG	565	0.0	31.099707	3
TCAACGC	580	0.0	30.295403	4
AGCTGTG	80	4.4772108E-4	29.681307	7
GTGTAGC	65	0.0056992583	29.361555	1
GTACATG	645	0.0	28.109707	1
ACATGGG	650	0.0	27.763441	3
CATGGGA	190	3.8926373E-10	27.494265	4
GAGTACT	380	0.0	27.494265	12-13
TACATGG	640	0.0	27.455208	2
>>END_MODULE