##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224510_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712444 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28212603376546 34.0 31.0 34.0 31.0 34.0 2 32.451031940756046 34.0 31.0 34.0 31.0 34.0 3 32.58756337340198 34.0 31.0 34.0 31.0 34.0 4 35.87797216342618 37.0 37.0 37.0 35.0 37.0 5 35.8941179938353 37.0 37.0 37.0 35.0 37.0 6 35.989336705762135 37.0 37.0 37.0 35.0 37.0 7 35.95287629624223 37.0 37.0 37.0 35.0 37.0 8 35.990277130553416 37.0 37.0 37.0 35.0 37.0 9 37.72207219093711 39.0 39.0 39.0 35.0 39.0 10-11 37.80048677510092 39.0 39.0 39.0 36.0 39.0 12-13 37.78467851508329 39.0 39.0 39.0 35.0 39.0 14-15 39.26756418750105 41.0 40.0 41.0 36.0 41.0 16-17 39.10876784701675 41.0 40.0 41.0 36.0 41.0 18-19 39.19411701130194 41.0 40.0 41.0 36.0 41.0 20-21 39.24825389785022 41.0 40.0 41.0 36.0 41.0 22-23 39.20342932216427 41.0 40.0 41.0 36.0 41.0 24-25 39.12892381717019 41.0 40.0 41.0 35.5 41.0 26-27 38.9471825153977 41.0 39.0 41.0 35.0 41.0 28-29 38.877772849515196 41.0 39.0 41.0 35.0 41.0 30-31 38.79462245453678 41.0 39.0 41.0 35.0 41.0 32-33 38.59899093823515 41.0 39.0 41.0 35.0 41.0 34-35 38.58187941789109 40.5 39.0 41.0 35.0 41.0 36-37 38.55408986530871 40.0 38.5 41.0 35.0 41.0 38-39 38.40518974122878 40.0 38.0 41.0 34.5 41.0 40-41 38.276703151405584 40.0 38.0 41.0 34.0 41.0 42-43 38.09594929566394 40.0 38.0 41.0 33.5 41.0 44-45 37.87197870990562 40.0 37.0 41.0 33.0 41.0 46-47 37.65331590974168 40.0 37.0 41.0 33.0 41.0 48-49 37.3863391368304 40.0 36.0 41.0 32.5 41.0 50-51 36.030284485517456 38.5 34.5 40.0 30.0 40.5 52-53 36.287228890972486 39.0 35.0 40.0 30.5 40.5 54-55 36.656170870973725 39.0 35.0 41.0 31.0 41.0 56-57 36.455725642997905 38.5 35.0 41.0 31.0 41.0 58-59 36.25859646512568 37.0 35.0 41.0 31.0 41.0 60-61 35.986803173302036 37.0 35.0 41.0 31.0 41.0 62-63 35.706784673602414 36.0 35.0 40.0 30.5 41.0 64-65 35.464035068019385 36.0 35.0 40.0 30.0 41.0 66-67 35.2699987648152 35.0 35.0 40.0 30.0 41.0 68-69 35.009889900118466 35.0 35.0 39.0 29.5 41.0 70-71 34.767672967980644 35.0 34.5 39.0 29.0 41.0 72-73 34.50245352617188 35.0 34.0 38.5 29.0 41.0 74-75 34.27450929476562 35.0 34.0 37.5 29.0 40.0 76-77 34.0086090976975 35.0 34.0 37.0 29.0 39.5 78-79 33.818085351269715 35.0 34.0 36.5 29.0 39.0 80-81 33.55061169720006 35.0 34.0 36.0 28.5 39.0 82-83 33.315844473390186 35.0 34.0 36.0 28.0 37.5 84-85 33.10466717384104 35.0 34.0 35.5 27.0 37.0 86-87 32.89633711561891 35.0 34.0 35.0 27.0 37.0 88-89 32.768471627243684 35.0 33.5 35.0 27.0 36.0 90-91 32.60029700579976 35.0 33.0 35.0 27.0 36.0 92-93 32.470142916495895 35.0 33.0 35.0 26.5 36.0 94-95 32.347495382093186 35.0 33.0 35.0 26.0 35.5 96-97 32.205070152882186 35.0 33.0 35.0 25.0 35.0 98-99 32.17230477062057 35.0 33.0 35.0 25.0 35.0 100-101 30.937265946516497 34.0 31.0 35.0 21.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 134.0 8 395.0 9 348.0 10 350.0 11 712.0 12 1069.0 13 1290.0 14 1279.0 15 1298.0 16 1487.0 17 1673.0 18 1905.0 19 2259.0 20 2760.0 21 3812.0 22 4643.0 23 4349.0 24 4140.0 25 4191.0 26 4362.0 27 4322.0 28 4644.0 29 5396.0 30 6629.0 31 8637.0 32 11668.0 33 16717.0 34 28748.0 35 54129.0 36 119418.0 37 181332.0 38 165463.0 39 62878.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.828106483505515 20.523251972632664 14.083465591916319 23.565175951945502 2 15.915917601944853 20.29184048149749 38.136611438934146 25.655630477623504 3 19.70639095844726 27.832222602758954 28.343561037779814 24.117825401013974 4 13.133383115023777 17.452038335644627 39.55735468331546 29.857223866016135 5 13.394175542218056 40.03739241259664 33.20752227543498 13.360909769750323 6 27.525110745546318 38.61215758712264 19.86766679205664 13.995064875274407 7 25.46572081454823 31.65989186518519 23.412927893280035 19.46145942698654 8 25.841890731060968 34.117348170522874 21.015406123147926 19.025354975268232 9 27.056582692815155 15.245829847679257 21.022704942423545 36.67488251708205 10-11 26.21286725693528 25.16450415751975 27.05412635940509 21.56850222613988 12-13 26.482081398678353 22.944399840548872 27.47844041075509 23.095078350017687 14-15 23.81072737701822 24.806629329997044 24.754745541258846 26.627897751725893 16-17 23.223228144599975 27.473433582199625 26.035651324984727 23.267686948215676 18-19 23.282581271976902 26.843392134963185 26.564980024668184 23.309046568391732 20-21 24.913184814890226 24.859614263949055 26.106621544769677 24.120579376391042 22-23 24.952858777186528 26.093799270039675 25.382660317027796 23.570681635746006 24-25 23.79476293962724 25.913649713326258 25.89041538210999 24.401171964936516 26-27 23.404365816822096 27.098691265559115 26.55668375338974 22.940259164229047 28-29 23.21375434420109 27.10711298010791 26.623987288825507 23.055145386865494 30-31 23.480512152534093 25.74490065184071 26.824073190313907 23.950514005311295 32-33 23.304357956555183 26.00078041221485 27.146554676578088 23.548306954651874 34-35 24.032485360252874 25.4062073650701 26.5757027920791 23.98560448259793 36-37 23.160240773564045 25.63985750136005 27.410139866275905 23.78976185879999 38-39 23.130209459212864 26.29961005381763 26.222827994149405 24.347352492820104 40-41 24.127613789734554 25.480225631825963 26.974541672193762 23.417618906245725 42-43 23.816622345931933 26.164393079648885 26.816325470701642 23.202659103717536 44-45 23.751143204679085 25.533735329604422 26.345401878119468 24.36971958759703 46-47 23.935074195305177 25.29019824716048 26.08345357670217 24.69127398083218 48-49 23.26119666949262 25.860839588795752 26.448043635710317 24.429920106001312 50-51 23.418261645827602 26.45260539775758 25.905123771131482 24.224009185283336 52-53 23.971287567864984 25.95874202042546 25.31539320985228 24.75457720185727 54-55 23.784325504881785 25.33778093436116 25.89754422803757 24.980349332719484 56-57 23.798993324387602 26.14542335959037 25.640471391435675 24.415111924586352 58-59 24.337224573282054 26.048307903749198 25.984853855640637 23.629613667328115 60-61 24.019528014488074 26.316701997725712 25.59278965618902 24.070980331597198 62-63 23.455241347259527 25.984687552212694 26.505656170540178 24.0544149299876 64-65 24.816453207504775 25.25299123693967 25.361054937647452 24.569500617908098 66-67 23.578861657360058 25.966594483421833 25.115066175773805 25.3394776834443 68-69 23.605013165947078 26.05145106141676 26.80842283744407 23.5351129351921 70-71 24.354756303653343 25.39680311715728 26.199883780339228 24.048556798850154 72-73 23.972621006001877 25.72139003205866 26.564965106029387 23.74102385591008 74-75 24.09950817187035 25.171873157749943 26.473659684129558 24.25495898625015 76-77 23.739409693954894 24.93950401715784 25.755147071208405 25.565939217678864 78-79 23.939810209970748 24.333737883670604 26.60177014755441 25.124681758804236 80-81 23.5214392104511 25.926349874262904 25.95064757588292 24.601563339403075 82-83 23.37906366341653 26.322862964892646 26.255594580892687 24.042478790798135 84-85 23.6613611600975 24.949944960517595 27.035969425005895 24.352724454379008 86-87 23.48070954059634 26.671465288408868 26.001952219772196 23.845872951222596 88-89 23.804719690698995 25.55777173535303 25.747399031012243 24.890109542935733 90-91 23.146661351629042 25.408523336571015 26.141914311861704 25.302900999938238 92-93 23.298545801633868 25.53002442379495 26.678784986384436 24.492644788186745 94-95 23.89310598447036 24.993613533133832 26.557596105799192 24.555684376596616 96-97 23.945320614672873 26.09741958666225 26.33575410839308 23.621505690271796 98-99 22.56766383016054 26.435441705412753 26.585911044828492 24.41098341959821 100-101 23.687874640938016 25.551521313413804 25.95357243415061 24.807031611497568 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.5 3 2.5 4 2.0 5 4.0 6 5.5 7 8.0 8 15.0 9 22.0 10 27.0 11 25.5 12 21.5 13 30.5 14 47.0 15 48.0 16 40.5 17 39.5 18 46.5 19 63.5 20 81.0 21 112.5 22 152.0 23 182.5 24 228.0 25 298.5 26 412.0 27 558.5 28 809.0 29 1227.0 30 1929.0 31 3475.0 32 6005.0 33 9455.5 34 13342.0 35 17437.0 36 23077.0 37 27003.0 38 26487.5 39 24248.5 40 20844.0 41 17517.5 42 15917.5 43 15479.5 44 15911.5 45 17909.5 46 20805.5 47 21368.5 48 23041.0 49 27391.5 50 31779.5 51 32024.0 52 30497.5 53 32296.0 54 35201.5 55 36064.5 56 29715.5 57 23650.0 58 22327.5 59 21503.0 60 17940.0 61 13127.0 62 10673.5 63 7507.0 64 4180.5 65 2559.0 66 2145.0 67 2052.5 68 1580.0 69 908.0 70 468.0 71 269.0 72 201.5 73 149.5 74 108.5 75 93.0 76 64.0 77 38.5 78 29.5 79 20.0 80 15.0 81 12.0 82 13.5 83 15.5 84 11.5 85 6.5 86 3.5 87 3.0 88 3.5 89 3.5 90 4.0 91 3.5 92 4.5 93 3.5 94 2.0 95 2.5 96 1.0 97 1.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15229267142399963 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.03866970596987272 16-17 0.07705868812145235 18-19 0.026738943692416528 20-21 0.04196821083481649 22-23 0.06842643070893993 24-25 0.019089219643929907 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.02196663878143405 38-39 0.005333752547568651 40-41 0.006246104957021183 42-43 0.0032985048649437714 44-45 0.011579857504589834 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.016492524324718854 60-61 0.018808495825636824 62-63 0.029897086648213753 64-65 0.05109173492934181 66-67 0.01915940059850318 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.018317229143623923 80-81 0.06211014479734547 82-83 0.05207426829336762 84-85 0.032002515285411905 86-87 0.06091706856959986 88-89 0.03614319160523494 90-91 0.0 92-93 0.003368685819517043 94-95 0.0 96-97 0.0 98-99 9.123524094525324E-4 100-101 0.04849503961013076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 712444.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.79871550810966 #Duplication Level Percentage of deduplicated Percentage of total 1 71.02277442592842 26.13546870696386 2 14.805239902880665 10.896276224308373 3 5.6314461540276115 6.216899547639011 4 2.655715400274107 3.9090766194076974 5 1.481593500611084 2.7260368863825786 6 0.9149028539697047 2.020034990447326 7 0.5858338150776606 1.509055232725135 8 0.38817001402241186 1.1427326331831738 9 0.30031237185581067 0.9945998581938852 >10 1.7484169887838998 12.239534314380046 >50 0.21262152179099894 5.533050719604712 >100 0.2230973990753587 17.09259065131888 >500 0.02250373764586227 5.500211917142858 >1k 0.007371914056403157 4.0844316983024616 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA 2620 0.3677482019639438 No Hit TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 2326 0.32648180067486005 No Hit ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA 2147 0.3013570189376288 No Hit TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA 2011 0.2822677992936989 No Hit GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC 1858 0.2607924271942777 No Hit GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC 1699 0.23847488363997732 No Hit CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA 1425 0.2000157205338244 No Hit GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG 1406 0.1973488442600401 No Hit CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA 1331 0.18682170107404933 No Hit ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA 1316 0.18471627243685118 No Hit GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC 1297 0.18204939616306687 No Hit GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC 1293 0.18148794852648067 No Hit GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT 1264 0.17741745316123092 No Hit ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG 1183 0.1660481385203609 No Hit GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA 1181 0.16576741470206782 No Hit GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA 1134 0.15917040497218027 No Hit TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC 1062 0.14906434751362915 No Hit ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT 1051 0.14752036651301717 No Hit ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA 1003 0.14078299487398307 No Hit TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG 998 0.14008118532825034 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 981 0.1376950328727591 No Hit GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC 956 0.13418598514409555 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC 937 0.1315191088703112 No Hit GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG 865 0.12141305141176008 No Hit CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG 848 0.11902689895626885 No Hit CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC 827 0.11607929886419144 No Hit TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA 824 0.1156582131367518 No Hit CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA 819 0.1149564035910191 No Hit CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT 817 0.114675679772726 No Hit GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT 813 0.11411423213613982 No Hit GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA 792 0.11116663204406242 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG 772 0.10835939386113155 No Hit GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG 771 0.10821903195198501 No Hit TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC 759 0.10653468904222647 No Hit GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT 744 0.10442926040502831 No Hit CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC 734 0.10302564131356289 No Hit ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA 731 0.10260455558612326 No Hit TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG 724 0.10162202222209747 No Hit GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC 714 0.10021840313063203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.807238182930869E-4 0.0 10-11 0.0 0.0 0.0 2.807238182930869E-4 0.0 12-13 0.0 0.0 0.0 2.807238182930869E-4 0.0 14-15 0.0 0.0 0.0 3.5090477286635867E-4 0.0 16-17 0.0 0.0 0.0 4.2108572743963037E-4 0.0 18-19 0.0 0.0 0.0 7.71990500305989E-4 0.0 20-21 0.0 0.0 0.0 0.0012632571823188912 0.0 22-23 0.0 0.0 0.0 0.0016843429097585215 0.0 24-25 0.0 0.0 0.0 0.00203524768262488 0.0 26-27 0.0 0.0 0.0 0.002596695319211054 0.0 28-29 0.0 0.0 0.0 0.0035090477286635864 0.0 30-31 0.0 0.0 0.0 0.006035562093301368 0.0 32-33 0.0 0.0 0.0 0.010456962231417486 0.0 34-35 0.0 0.0 0.0 0.01705397196130503 0.0 36-37 0.0 0.0 0.0 0.028633829465894864 0.0 38-39 0.0 0.0 0.0 0.05109173492934181 0.0 40-41 0.0 0.0 0.0 0.08225207875987446 0.0 42-43 0.0 0.0 0.0 0.107447041451679 0.0 44-45 0.0 0.0 0.0 0.1422567949200218 0.0 46-47 0.0 0.0 0.0 0.1819090342539203 0.0 48-49 0.0 0.0 0.0 0.22626339754422803 0.0 50-51 0.0 0.0 0.0 0.2724424656534408 0.0 52-53 0.0 0.0 0.0 0.31953388617210615 0.0 54-55 0.0 0.0 0.0 0.37259068782949956 0.0 56-57 0.0 0.0 0.0 0.4354026421725778 0.0 58-59 0.0 0.0 0.0 0.5046010633818236 0.0 60-61 0.0 0.0 0.0 0.5840459039587673 0.0 62-63 0.0 0.0 0.0 0.669175401856146 0.0 64-65 0.0 0.0 0.0 0.7526907377983392 0.0 66-67 0.0 0.0 0.0 0.8468033978810967 0.0 68-69 0.0 0.0 0.0 0.9593034680620511 0.0 70-71 0.0 0.0 0.0 1.085769548203087 0.0 72-73 0.0 0.0 0.0 1.2290088764871343 0.0 74-75 0.0 0.0 0.0 1.3721780238166088 0.0 76-77 0.0 0.0 0.0 1.512750475826872 0.0 78-79 0.0 0.0 0.0 1.6891152146695039 0.0 80-81 0.0 0.0 0.0 1.890394192385647 0.0 82-83 0.0 0.0 0.0 2.106762075335044 0.0 84-85 0.0 0.0 0.0 2.3324640252426856 0.0 86-87 0.0 0.0 0.0 2.582869671160119 0.0 88-89 0.0 0.0 0.0 2.849627479493125 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 690 0.0 62.687607 1 GCGTAAT 25 0.005006977 57.038834 1 AAACCGT 270 0.0 56.26348 6 CCGTGCG 280 0.0 56.00848 9 ACCGTGC 305 0.0 54.480247 8 AACCGTG 295 0.0 53.10462 7 AGGGCGC 40 5.4004305E-4 47.475643 8 GTATCAA 1535 0.0 44.900276 1 AATGCGG 310 0.0 36.752758 7 ATGCGGG 320 0.0 34.12312 8 AAATGCG 345 0.0 33.024216 6 AATGCCG 145 6.6029315E-10 32.77403 9 ATCAACG 2090 0.0 32.708195 3 TCAACGC 2250 0.0 30.382282 4 GTCTAAT 610 0.0 28.051886 1 CGCAGAG 2540 0.0 27.289148 8 GTACATG 4150 0.0 27.144987 1 GCCCATA 1055 0.0 27.032621 1 TATACCG 125 7.419263E-6 26.584494 5 CCCTTAC 90 8.9086895E-4 26.40687 1 >>END_MODULE