##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224510_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712444 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.15792539483805 34.0 33.0 34.0 31.0 34.0 2 33.28583018454784 34.0 33.0 34.0 31.0 34.0 3 33.40420720786476 34.0 34.0 34.0 31.0 34.0 4 36.625379678964244 37.0 37.0 37.0 35.0 37.0 5 36.6597753648006 37.0 37.0 37.0 35.0 37.0 6 36.699507610422714 37.0 37.0 37.0 36.0 37.0 7 36.6796099061821 37.0 37.0 37.0 36.0 37.0 8 36.670225308936566 37.0 37.0 37.0 36.0 37.0 9 38.51034747994228 39.0 39.0 39.0 37.0 39.0 10-11 38.53527786043534 39.0 39.0 39.0 37.5 39.0 12-13 38.508764899416654 39.0 39.0 39.0 37.0 39.0 14-15 40.08443189920892 41.0 40.0 41.0 38.0 41.0 16-17 40.09578577403978 41.0 40.0 41.0 38.0 41.0 18-19 40.09250762165166 41.0 40.0 41.0 38.0 41.0 20-21 40.08900559201846 41.0 40.0 41.0 38.0 41.0 22-23 40.01776420322159 41.0 40.0 41.0 38.0 41.0 24-25 39.963244128661344 41.0 40.0 41.0 38.0 41.0 26-27 39.878377107534064 41.0 40.0 41.0 38.0 41.0 28-29 39.84766171095553 41.0 40.0 41.0 37.5 41.0 30-31 39.77201997630691 41.0 40.0 41.0 37.0 41.0 32-33 39.714677223753725 41.0 40.0 41.0 37.0 41.0 34-35 39.62555232411249 41.0 40.0 41.0 36.5 41.0 36-37 39.48053601405865 41.0 39.5 41.0 36.0 41.0 38-39 39.331784673602414 41.0 39.0 41.0 35.5 41.0 40-41 39.17075236790541 41.0 39.0 41.0 35.0 41.0 42-43 39.031650908703 41.0 39.0 41.0 35.0 41.0 44-45 38.73059005339367 40.5 38.0 41.0 35.0 41.0 46-47 38.45585056509705 40.0 37.5 41.0 35.0 41.0 48-49 38.28872514892399 40.0 37.0 41.0 35.0 41.0 50-51 38.054868873904475 40.0 36.5 41.0 34.5 41.0 52-53 37.73713934007445 39.5 36.0 41.0 34.0 41.0 54-55 37.492105344420054 39.0 35.0 41.0 34.0 41.0 56-57 37.20808653030975 39.0 35.0 41.0 33.5 41.0 58-59 36.89577145712505 37.5 35.0 41.0 33.0 41.0 60-61 36.6316033260158 37.0 35.0 41.0 33.0 41.0 62-63 36.433974459747006 36.5 35.0 40.5 33.0 41.0 64-65 36.17583697806424 36.0 35.0 40.0 33.0 41.0 66-67 35.951787087827256 35.5 35.0 40.0 33.0 41.0 68-69 35.72898010229576 35.0 35.0 39.5 32.0 41.0 70-71 35.48567115450477 35.0 35.0 39.0 32.0 41.0 72-73 35.24176707221901 35.0 35.0 39.0 32.0 41.0 74-75 34.98210104934563 35.0 35.0 37.5 31.0 40.5 76-77 33.18446362100039 34.5 32.0 35.5 28.5 38.5 78-79 34.5533606851907 35.0 34.5 37.0 31.5 39.0 80-81 34.51444253864163 35.0 35.0 36.0 32.0 39.0 82-83 34.333906945668716 35.0 35.0 36.0 32.0 38.0 84-85 34.13224127089287 35.0 35.0 36.0 32.0 37.0 86-87 33.949040205265256 35.0 35.0 35.5 31.5 37.0 88-89 33.82161545328475 35.0 34.5 35.0 31.0 36.0 90-91 33.67962253875392 35.0 34.0 35.0 31.0 36.0 92-93 33.57270536350927 35.0 34.0 35.0 31.0 36.0 94-95 33.47125809186406 35.0 34.0 35.0 31.0 36.0 96-97 33.42869404472492 35.0 34.0 35.0 31.0 35.5 98-99 33.423821381048896 35.0 34.0 35.0 31.0 35.0 100-101 32.56688736237515 34.5 33.0 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 13.0 15 41.0 16 109.0 17 263.0 18 469.0 19 759.0 20 1038.0 21 1704.0 22 2468.0 23 2430.0 24 1953.0 25 2294.0 26 3075.0 27 4211.0 28 5539.0 29 6203.0 30 6275.0 31 6566.0 32 7497.0 33 10039.0 34 17834.0 35 39036.0 36 116257.0 37 209403.0 38 186758.0 39 80200.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.279088725566936 20.769390250610343 14.106193117629887 22.845327906192836 2 15.658353498660949 20.52217437440697 39.08433504949161 24.735137077440474 3 19.176244027600767 28.848162101161634 28.725485792567557 23.250108078670042 4 13.122855971837785 17.70160181010718 40.355452498722705 28.820089719332326 5 13.213810488964747 39.15928830897587 34.39133461717693 13.235566584882461 6 27.277231613993518 38.10474928555788 20.544913003688713 14.073106096759885 7 25.359326487415153 31.574411462514952 23.82924131580868 19.237020734261222 8 25.88161315134944 33.99088209038184 20.867605032816613 19.259899725452108 9 27.081988198370677 15.370471223001386 21.484074537788235 36.06346604083971 10-11 26.556192486707726 24.98091078035607 27.367203597756458 21.095693135179747 12-13 26.799580037167836 23.363029234578438 27.642242758729108 22.195147969524623 14-15 23.9573215579049 25.35399273486758 25.15383665242461 25.534849054802905 16-17 22.967419193648904 27.580413337750052 26.391828691097125 23.060338777503915 18-19 23.020054909578857 26.784350770025434 27.492476601669747 22.703117718725963 20-21 24.713641145561745 25.13255567338282 26.911731782411486 23.242071398643947 22-23 24.9277996708437 26.100340034600013 26.010646697383244 22.961213597173046 24-25 24.273852660183326 25.621963347166048 26.263419522866087 23.84076446978454 26-27 23.50417452724251 27.231420967524596 26.766032767371872 22.498371737861024 28-29 23.401385180755703 26.798994382353687 27.268254543106522 22.53136589378409 30-31 23.524453596207913 25.382458090678256 27.575582758189913 23.517505554923915 32-33 23.524581650155383 25.885475589591856 27.847179138793056 22.742763621459712 34-35 24.264142865665146 25.577207185393398 26.766958981215367 23.391690967726085 36-37 23.214486235802074 25.618869555941092 27.356589016714672 23.810055191542162 38-39 23.297410042052427 26.146476073908964 26.566017820347987 23.99009606369062 40-41 23.67154471088254 25.86357664602411 27.291899433499335 23.172979209594015 42-43 23.63652441455048 26.22051698098377 27.267827366080706 22.875131238385052 44-45 23.989915473305274 25.536986307138694 26.905360001740647 23.56773821781539 46-47 23.804312629462117 25.256325337382933 26.39380084977021 24.545561183384738 48-49 23.292204232968363 25.234426046574303 27.14310077530261 24.330268945154724 50-51 23.944688986081715 25.653384687077157 26.46418525526217 23.93774107157896 52-53 24.061189370673343 25.863225741251245 25.980708659206897 24.094876228868515 54-55 24.10589463873652 25.095726822037946 26.408531758285562 24.389846780939976 56-57 24.384897698775166 25.828295653382348 25.589519098965933 24.19728754887655 58-59 24.74462625106632 25.608708931147913 26.152139494553406 23.49452532323236 60-61 24.015571140736995 26.121255519751763 26.01658207142506 23.846591268086183 62-63 23.874157126735575 25.590362189870362 26.722661710955524 23.812818972438535 64-65 24.51547068962613 25.441087299493013 25.89515807558208 24.148283935298775 66-67 23.854576640409633 25.661455496853087 25.84315398824329 24.640813874493993 68-69 24.212639870642462 25.72658342269708 26.591774230676375 23.469002475984077 70-71 24.842163033164713 25.386697059698726 26.245361038902708 23.525778868233854 72-73 24.050241141759916 25.515689654204397 26.61016164077457 23.823907563261113 74-75 24.3075947021801 24.874586634177565 26.422778492063937 24.395040171578398 76-77 23.96560000673739 24.476799514907878 26.423414116517556 25.134186361837173 78-79 24.00534543918089 24.444715834038714 26.407002974523536 25.14293575225686 80-81 23.257142576401897 25.775038057946336 26.467555820075926 24.500263545575837 82-83 23.524416493493824 25.69625308009042 26.745747176773854 24.0335832496419 84-85 23.87732357139749 24.912376987396602 27.025575953746838 24.184723487459067 86-87 23.33088635738388 26.377511776364177 26.447622549982874 23.843979316269067 88-89 24.074593932996837 25.067514078299485 25.68594864999916 25.171943338704516 90-91 23.803555086434862 25.351887306230385 25.949688677285515 24.89486893004924 92-93 23.96774198076197 25.450246546039473 26.551738174141647 24.030273299056905 94-95 24.012764512017785 25.003930133456105 26.130194092447965 24.853111262078144 96-97 24.01290487392693 25.95909292519833 26.368668976087946 23.659333224786792 98-99 22.03639865027988 26.537805076609526 26.93832778435919 24.487468488751396 100-101 23.39303798446237 25.59572385100618 26.43468843976184 24.57654972476961 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.5 3 3.0 4 2.0 5 3.0 6 5.0 7 11.5 8 24.0 9 25.5 10 24.0 11 31.5 12 35.5 13 37.0 14 42.5 15 45.5 16 51.0 17 67.0 18 75.0 19 82.0 20 98.5 21 122.0 22 157.5 23 198.5 24 273.0 25 380.5 26 516.0 27 699.0 28 966.5 29 1406.0 30 2334.0 31 4196.0 32 7148.5 33 10844.5 34 14449.5 35 18599.0 36 24286.0 37 27756.0 38 26320.5 39 23108.5 40 19693.0 41 16605.0 42 15237.5 43 15306.5 44 16150.0 45 17917.5 46 19712.5 47 21376.0 48 23362.5 49 27737.0 50 33114.0 51 32486.5 52 29274.0 53 31010.5 54 34907.0 55 34905.5 56 28655.5 57 23468.0 58 22656.5 59 22506.5 60 18752.5 61 13174.5 62 10353.0 63 7040.0 64 3816.5 65 2217.0 66 1839.5 67 1760.5 68 1282.0 69 718.0 70 331.0 71 179.0 72 145.5 73 108.0 74 68.5 75 57.5 76 41.5 77 19.5 78 7.5 79 4.5 80 4.5 81 5.5 82 4.0 83 0.5 84 1.0 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47933591973544587 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0010527143185990759 22-23 0.002315971500917967 24-25 3.509047728663586E-4 26-27 0.0039301334561032165 28-29 0.006456647820740998 30-31 0.0018247048189050648 32-33 2.807238182930869E-4 34-35 0.0037195905923834015 36-37 0.0022457905463446953 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.009193705049098595 46-47 0.08484877407908552 48-49 0.0017545238643317932 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008772619321658966 58-59 0.04231911560768285 60-61 0.03214287719455845 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.807238182930869E-4 78-79 0.00856207645793915 80-81 0.006105743047874639 82-83 7.719905003059891E-4 84-85 0.0023861524554912384 86-87 0.0 88-89 0.0 90-91 0.0 92-93 1.4036190914654346E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.069479145027539 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 712444.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.95400394760949 #Duplication Level Percentage of deduplicated Percentage of total 1 69.5942778053715 24.325986611399873 2 15.32967803154306 10.716672528602771 3 5.885238284978853 6.1713792673732 4 2.8724053719617704 4.016082748427457 5 1.5982807509384067 2.7933155838844663 6 0.9506609472912678 1.9937643902674291 7 0.6155746542099143 1.5061759225311184 8 0.4320877437453596 1.208255736047118 9 0.3270144012580864 1.0287416405250276 >10 1.886521727058611 12.430818395487952 >50 0.235201271607906 5.853259640810818 >100 0.24083965781874023 18.11245927390799 >500 0.02255354655158771 5.123203487835147 >1k 0.009665805664966162 4.719884772899645 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC 2061 0.289285894751026 No Hit GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT 2049 0.2876015518412675 No Hit TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 1898 0.26640690356013946 No Hit CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG 1698 0.23833452173083078 No Hit TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG 1644 0.2307549786369174 No Hit ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA 1573 0.22078928308751286 No Hit GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT 1514 0.21250793044786678 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG 1510 0.2119464828112806 No Hit GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA 1434 0.20127897771614328 No Hit ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT 1405 0.19720848235089353 No Hit GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG 1391 0.19524341562284195 No Hit ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG 1362 0.19117292025759217 No Hit GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA 1268 0.1779789007978171 No Hit GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA 1238 0.1737680435234208 No Hit GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC 1214 0.17039935770390374 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1213 0.1702589957947572 No Hit TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC 1199 0.1682939290667056 No Hit GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG 1188 0.1667499480660936 No Hit CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA 1179 0.16548669088377474 No Hit ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT 1133 0.15903004306303373 No Hit ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA 1133 0.15903004306303373 No Hit TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA 1120 0.15720533824412866 No Hit GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT 1072 0.1504679666050946 No Hit GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC 1032 0.14485349023923283 No Hit GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT 995 0.13966009960081074 No Hit CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA 938 0.13165947077945778 No Hit GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC 926 0.12997512786969923 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC 860 0.12071124186602737 No Hit GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA 848 0.11902689895626885 No Hit ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC 842 0.11818472750138957 No Hit ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG 839 0.11776364177394996 No Hit GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC 838 0.11762327986480342 No Hit GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC 836 0.11734255604651032 No Hit TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT 824 0.1156582131367518 No Hit GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG 805 0.11299133686296747 No Hit GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG 794 0.11144735586235549 No Hit GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT 762 0.10695577476966611 No Hit GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC 750 0.10527143185990759 No Hit GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT 743 0.10428889849588178 No Hit GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC 728 0.10218346985868362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.018095457327173E-5 0.0 16-17 0.0 0.0 0.0 1.4036190914654346E-4 0.0 18-19 0.0 0.0 0.0 4.912666820129021E-4 0.0 20-21 0.0 0.0 0.0 9.825333640258041E-4 0.0 22-23 0.0 0.0 0.0 0.0014036190914654344 0.0 24-25 0.0 0.0 0.0 0.0017545238643317932 0.0 26-27 0.0 0.0 0.0 0.002315971500917967 0.0 28-29 0.0 0.0 0.0 0.0032283239103704994 0.0 30-31 0.0 0.0 0.0 0.005754838275008282 0.0 32-33 0.0 0.0 0.0 0.0101762384131244 0.0 34-35 0.0 0.0 0.0 0.016843429097585214 0.0 36-37 0.0 0.0 0.0 0.02877419137504141 0.0 38-39 0.0 0.0 0.0 0.05172336352050126 0.0 40-41 0.0 0.0 0.0 0.08323461212390026 0.0 42-43 0.0 0.0 0.0 0.10892084149771772 0.0 44-45 0.0 0.0 0.0 0.14415168069350012 0.0 46-47 0.0 0.0 0.0 0.1845759105277046 0.0 48-49 0.0 0.0 0.0 0.2295619024091718 0.0 50-51 0.0 0.0 0.0 0.27602169433667767 0.0 52-53 0.0 0.0 0.0 0.323885105355649 0.0 54-55 0.0 0.0 0.0 0.3773629927404821 0.0 56-57 0.0 0.0 0.0 0.44017494708356025 0.0 58-59 0.0 0.0 0.0 0.5102155397476854 0.0 60-61 0.0 0.0 0.0 0.5898007422337757 0.0 62-63 0.0 0.0 0.0 0.6763338592226196 0.0 64-65 0.0 0.0 0.0 0.7618142618928646 0.0 66-67 0.0 0.0 0.0 0.8576814458399538 0.0 68-69 0.0 0.0 0.0 0.970602601748348 0.0 70-71 0.0 0.0 0.0 1.099033748617435 0.0 72-73 0.0 0.0 0.0 1.2445890484024007 0.0 74-75 0.0 0.0 0.0 1.390565433914806 0.0 76-77 0.0 0.0 0.0 1.532892409789401 0.0 78-79 0.0 0.0 0.0 1.7125556534969766 0.0 80-81 0.0 0.0 0.0 1.9162207836686111 0.0 82-83 0.0 0.0 0.0 2.1344835523914862 0.0 84-85 0.0 0.0 0.0 2.3624312928454727 0.0 86-87 0.0 0.0 0.0 2.618451415128768 0.0 88-89 0.0 0.0 0.0 2.8907535188730624 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 20 0.002076361 71.22742 3 GGTATCA 615 0.0 67.486435 1 CCGTGCG 265 0.0 57.340313 9 AAACCGT 280 0.0 54.26851 6 GTATCAA 1665 0.0 54.15268 1 ACCGTGC 290 0.0 54.034595 8 AACCGTG 290 0.0 50.75977 7 ATCAACG 2175 0.0 40.171173 3 TCAACGC 2390 0.0 36.55745 4 AATGCGG 270 0.0 33.415333 7 GTCTAAT 650 0.0 33.027184 1 TATCAAC 2700 0.0 32.887722 2 CGCAGAG 2790 0.0 31.65663 8 ACGCAGA 2925 0.0 30.195555 7 TTCCGTG 80 4.5002913E-4 29.678091 7 CAACGCA 2990 0.0 29.221506 5 AAATGCG 325 0.0 29.221506 6 CGAGTGG 180 2.1100277E-10 29.018578 3 ATGCGGG 295 0.0 28.973867 8 CAAAACC 595 0.0 28.730389 4 >>END_MODULE