FastQCFastQC Report
Fri 10 Feb 2017
SRR3224508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963650
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG26470.27468479219633685No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA19570.20308203185803975No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA18270.18959165672183884No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA14030.14559227935453742No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA12780.13262076480049811No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG12350.12815856379390858No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA12280.12743215897888238No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA11470.1190266175478649No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC11300.11726249156851555No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC11240.11663985886992165No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA10580.10979089918538888No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT10460.1085456337882011No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC10240.1062626472266902No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG10190.10574378664452862No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA10020.10397966066517927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.039.5510031
GGTATCA7100.038.192521
CCGTGCG1003.0249794E-838.007149
ATAACCG1003.0388037E-837.9874235
TAACCGT1203.805326E-935.613216
ATCAACG14550.033.61433
AACCGTG2500.032.289317
TCAACGC15150.032.283044
TCGCACC10800.031.2749351
CGCAGAG16750.029.4843048
CTGTGCG12400.029.1183769
CGCACCA11800.028.5710492
ACGCAGA17900.027.588637
GTACATG32350.026.9115431
ACATGGG31000.026.0397643
TACATGG33000.025.4688422
GCACCAC14100.025.2575953
CGTTATA950.00121545925.0384941
AAACCGT1901.1947122E-824.9917246
ACACGCT5750.024.7744066
Produced by FastQC (version 0.11.5)