##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224508_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963650
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.195334405645205	34.0	31.0	34.0	31.0	34.0
2	32.358402947128106	34.0	31.0	34.0	31.0	34.0
3	32.495773361697715	34.0	31.0	34.0	31.0	34.0
4	35.73156540237638	37.0	37.0	37.0	35.0	37.0
5	35.75683806361231	37.0	37.0	37.0	35.0	37.0
6	35.84968401390547	37.0	37.0	37.0	35.0	37.0
7	35.81775229595807	37.0	37.0	37.0	35.0	37.0
8	35.851590307684326	37.0	37.0	37.0	35.0	37.0
9	37.5975188086961	39.0	39.0	39.0	35.0	39.0
10-11	37.672297514657814	39.0	39.0	39.0	36.0	39.0
12-13	37.67724744461164	39.0	39.0	39.0	37.0	39.0
14-15	39.19428267524516	41.0	40.0	41.0	37.0	41.0
16-17	39.04142012141338	41.0	40.0	41.0	36.5	41.0
18-19	39.12364810875318	41.0	40.0	41.0	36.5	41.0
20-21	39.18965651429461	41.0	40.0	41.0	37.0	41.0
22-23	39.15274529134022	41.0	40.0	41.0	37.0	41.0
24-25	39.107808332900944	41.0	40.0	41.0	36.0	41.0
26-27	38.958246251232296	41.0	40.0	41.0	36.0	41.0
28-29	38.89934934882997	41.0	40.0	41.0	35.5	41.0
30-31	38.83692678877186	41.0	39.0	41.0	35.0	41.0
32-33	38.6610589944482	41.0	39.0	41.0	35.0	41.0
34-35	38.67403102786281	41.0	39.0	41.0	35.0	41.0
36-37	38.66614694131687	41.0	39.0	41.0	35.0	41.0
38-39	38.5518383230426	41.0	39.0	41.0	35.0	41.0
40-41	38.47615576194677	41.0	39.0	41.0	35.0	41.0
42-43	38.34909977688994	40.5	38.5	41.0	34.5	41.0
44-45	38.19696310901261	40.0	38.0	41.0	34.0	41.0
46-47	38.059769107040935	40.0	38.0	41.0	34.0	41.0
48-49	37.88537332018886	40.0	38.0	41.0	33.0	41.0
50-51	36.64658849167229	39.0	36.0	40.0	31.5	40.5
52-53	36.93848907798474	39.5	36.5	40.5	32.5	40.5
54-55	37.39441654127536	40.0	36.0	41.0	33.0	41.0
56-57	37.27530898147668	40.0	35.5	41.0	33.0	41.0
58-59	37.15677839464536	40.0	35.0	41.0	33.0	41.0
60-61	36.95258963316557	39.5	35.0	41.0	32.5	41.0
62-63	36.720378768224975	39.0	35.0	41.0	32.0	41.0
64-65	36.515000259430295	39.0	35.0	41.0	32.0	41.0
66-67	36.32997353811031	38.5	35.0	41.0	31.0	41.0
68-69	36.085341150832775	37.5	35.0	41.0	31.0	41.0
70-71	35.838087479894156	37.0	35.0	40.0	31.0	41.0
72-73	35.54780210657397	36.5	35.0	40.0	30.5	41.0
74-75	35.26063508535257	36.0	35.0	39.0	30.0	41.0
76-77	34.91650495511856	36.0	35.0	39.0	30.0	41.0
78-79	34.63099361801484	35.0	35.0	38.5	30.0	39.5
80-81	34.27298241062627	35.0	34.5	37.0	29.5	39.0
82-83	33.95052145488507	35.0	34.0	37.0	29.0	39.0
84-85	33.65425413791314	35.0	34.0	36.5	29.0	38.0
86-87	33.3736154205365	35.0	34.0	36.0	29.0	37.0
88-89	33.17516629481658	35.0	34.0	36.0	29.0	37.0
90-91	32.95554558190214	35.0	34.0	35.0	27.5	36.0
92-93	32.77560992061433	35.0	34.0	35.0	27.0	36.0
94-95	32.61504799460385	35.0	34.0	35.0	27.0	36.0
96-97	32.46582421003477	35.0	34.0	35.0	26.5	36.0
98-99	32.39017641259794	35.0	33.0	35.0	26.0	35.5
100-101	31.21331655684118	34.5	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	846.0
8	3995.0
9	2635.0
10	1676.0
11	1734.0
12	1813.0
13	1841.0
14	1709.0
15	1635.0
16	1704.0
17	1789.0
18	2002.0
19	2284.0
20	2680.0
21	3464.0
22	3999.0
23	4034.0
24	3888.0
25	4342.0
26	4349.0
27	4602.0
28	5140.0
29	5673.0
30	7161.0
31	9052.0
32	12168.0
33	17515.0
34	29212.0
35	54330.0
36	117034.0
37	202200.0
38	305839.0
39	141237.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10439968156507	20.81290285032519	14.007083722196704	24.07561374591304
2	15.128106677735692	21.159030768432523	39.775437140040474	23.937425413791313
3	19.085767654231308	27.649250246458777	29.41088569501375	23.854096404296165
4	12.293467545270586	18.253515280444145	40.676386654905826	28.77663051937944
5	12.904996627406215	39.38369740050848	35.33741503657967	12.37389093550563
6	27.18362476002698	39.18902091008146	20.68769781559695	12.93965651429461
7	25.021013853577546	31.925491620401598	24.582265345301717	18.47122918071914
8	23.740569708919214	36.14299797644373	22.394852903024955	17.7215794116121
9	25.90058631245784	16.120686971410784	21.349763918435116	36.62896279769626
10-11	24.824832667462253	26.037409847973848	29.26358117573808	19.87417630882582
12-13	25.12976703159861	23.85954444040886	29.214081876199867	21.796606651792665
14-15	22.16332818443038	26.04296427325726	26.212275981198204	25.581431561114155
16-17	22.11664574175439	28.489969022822176	27.51304042609975	21.880344809323685
18-19	22.04634791661693	28.215327031406183	27.95100523246098	21.787319819515908
20-21	23.148740739356587	26.70901141771867	27.525801075176727	22.61644676774802
22-23	23.211653592528194	27.369712476253422	26.90711174637227	22.511522184846115
24-25	22.541212027487486	27.311459450146497	27.35878724598933	22.788541276376684
26-27	22.428111866341514	28.300627821304413	27.49104965495771	21.780210657396356
28-29	22.369843822964768	28.13241322056763	27.524516162507133	21.973226793960464
30-31	22.275255538836713	27.42546567737249	27.742178176723915	22.557100607066882
32-33	22.33767446687075	27.594458568982517	28.022155346858302	22.045711617288436
34-35	22.871789550147874	27.049758729829293	27.59938774451305	22.47906397550978
36-37	21.87313332527198	27.253671591876856	28.381603714588643	22.49159136826252
38-39	22.253678254259405	27.633905059399495	27.323247916023462	22.789168770317637
40-41	22.684099129881606	27.093273225685433	27.9390559093487	22.28357173508426
42-43	22.502835652532163	27.498461535268458	27.897735940300304	22.10096687189907
44-45	22.550411004255597	26.937500681072006	27.79422440557332	22.71786390909907
46-47	22.45068230166554	26.828776007886677	27.862501945727182	22.858039744720593
48-49	21.86509624863799	27.404607481969595	27.985212473408396	22.74508379598402
50-51	22.03279198879261	27.675400819799723	27.589736937684844	22.702070253722827
52-53	22.27556685518601	27.334665075494215	27.18616717687957	23.2036008924402
54-55	22.29331188709594	26.745913972915474	27.714834224044	23.245939915944586
56-57	22.330773621128003	27.150365796710425	27.546827167540084	22.97203341462149
58-59	22.518589400629672	27.35512877528685	27.716771689623304	22.409510134460177
60-61	22.31458311494347	27.53071902072775	27.507989176857905	22.646708687470873
62-63	21.96585792195444	27.067551324183658	28.062375828587914	22.90421492527398
64-65	22.80018189066627	26.51072033687148	27.45422625870002	23.23487151376223
66-67	22.045549960741738	27.071593262628678	27.17346235021274	23.70939442641684
68-69	22.238520209619676	27.134333004721633	28.183572873968764	22.443573911689928
70-71	22.66974523945416	26.88834120271883	27.666787734135838	22.775125823691177
72-73	22.358843978622943	27.189384112488973	27.9062418928034	22.545530016084676
74-75	22.44238053235096	26.982099309915426	27.819488403465986	22.756031754267628
76-77	22.39615005448036	26.615628080734705	27.26425569449489	23.72396617029004
78-79	22.672267102163836	26.196358782828998	27.708766975282213	23.42260713972495
80-81	22.221258918276583	27.319686373615763	27.277531731491543	23.181522976616115
82-83	22.039621733553368	27.564218033220044	27.58254268267677	22.813617550549818
84-85	22.380816946788016	26.605473161496047	28.091680229854514	22.922029661861423
86-87	22.373648121075192	27.478185118118088	27.469307572183272	22.678859188623452
88-89	22.670108860951338	26.742818467849844	27.046034677304664	23.541037993894154
90-91	22.433092927930264	26.614746017745034	27.378145592279356	23.57401546204535
92-93	22.386376844080264	26.828877238424948	27.751955119424178	23.032790798070618
94-95	22.736003735796192	26.454469983915324	27.703159860945366	23.10636641934312
96-97	23.124370881544127	26.98407098012764	27.43791833134437	22.453639806983862
98-99	22.15460864259282	27.092677186435683	27.560743260491922	23.191970910479576
100-101	22.933473000771873	26.509163599329554	27.170005829281475	23.387357570617098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	3.5
6	6.0
7	9.5
8	16.0
9	19.5
10	16.0
11	20.5
12	27.5
13	23.5
14	25.0
15	36.5
16	36.5
17	38.5
18	55.5
19	67.5
20	85.5
21	95.5
22	99.5
23	122.0
24	169.5
25	220.5
26	293.0
27	456.5
28	722.5
29	1469.0
30	3159.5
31	6176.5
32	10552.5
33	16500.0
34	23952.0
35	32266.0
36	40050.0
37	45853.0
38	50202.5
39	52298.5
40	50970.0
41	47181.0
42	41923.0
43	36690.5
44	31759.0
45	28744.5
46	28196.5
47	25861.5
48	24413.5
49	26637.0
50	29455.0
51	29485.0
52	28737.0
53	29590.5
54	31653.0
55	32492.0
56	26945.0
57	22136.0
58	21370.0
59	20768.0
60	17443.0
61	12858.0
62	10611.5
63	7554.0
64	4219.0
65	2526.5
66	2045.5
67	1889.0
68	1472.5
69	948.5
70	518.5
71	307.5
72	266.5
73	207.5
74	157.0
75	121.5
76	87.5
77	62.5
78	39.0
79	28.5
80	23.5
81	16.0
82	13.0
83	10.5
84	7.0
85	6.5
86	8.0
87	7.0
88	4.5
89	2.0
90	3.0
91	6.5
92	4.0
93	0.5
94	3.0
95	4.0
96	2.5
97	1.5
98	1.5
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14984693612826233
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03507497535412235
16-17	0.07087635552327089
18-19	0.025009080060187826
20-21	0.03803248067244332
22-23	0.06397550978052198
24-25	0.016292222279873397
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.021325169926840658
38-39	0.004929175530534945
40-41	0.004721631297670316
42-43	0.0028018471436724954
44-45	0.009910237119286049
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.01354226119441706
60-61	0.016499766512738025
62-63	0.027395838738131065
64-65	0.045037098531624555
66-67	0.01644788045452187
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.015929019872360298
80-81	0.054947335650910596
82-83	0.048409692315674775
84-85	0.026773206039537176
86-87	0.056659575572043784
88-89	0.03227312821044986
90-91	0.0
92-93	0.0026980750272401805
94-95	0.0
96-97	0.0
98-99	4.150884657292586E-4
100-101	0.04249468167903284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	963650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.09332552426283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.30094136292605	27.384118756773816
2	15.161458996112684	12.157466219078179
3	6.226961639662938	7.489788001383109
4	3.122880345775764	5.0082663310604065
5	1.8312167822798517	3.670978527871961
6	1.1632452085516662	2.798302128660058
7	0.7600018152388197	2.132970012418046
8	0.5651694825410868	1.8127619231919176
9	0.4169113319695914	1.5043825572669751
>10	2.1379152811415265	15.38406669818123
>50	0.16110320618185006	4.556059065638017
>100	0.13700128813682716	11.07431606293078
>500	0.01126396823625406	2.912884146482535
>1k	0.0039292912452049055	2.1136395690629537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2647	0.27468479219633685	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1957	0.20308203185803975	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1827	0.18959165672183884	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1403	0.14559227935453742	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1278	0.13262076480049811	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1235	0.12815856379390858	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1228	0.12743215897888238	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1147	0.1190266175478649	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1130	0.11726249156851555	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1124	0.11663985886992165	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1058	0.10979089918538888	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1046	0.1085456337882011	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1024	0.1062626472266902	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1019	0.10574378664452862	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1002	0.10397966066517927	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0377211643231463E-4	0.0
3	0.0	0.0	0.0	2.0754423286462926E-4	0.0
4	0.0	0.0	0.0	2.0754423286462926E-4	0.0
5	0.0	0.0	0.0	2.0754423286462926E-4	0.0
6	0.0	0.0	0.0	2.0754423286462926E-4	0.0
7	0.0	0.0	0.0	2.0754423286462926E-4	0.0
8	0.0	0.0	0.0	2.0754423286462926E-4	0.0
9	0.0	0.0	0.0	3.113163492969439E-4	0.0
10-11	0.0	0.0	0.0	3.113163492969439E-4	0.0
12-13	0.0	0.0	0.0	3.113163492969439E-4	0.0
14-15	0.0	0.0	0.0	5.188605821615732E-4	0.0
16-17	0.0	0.0	0.0	7.264048150262025E-4	0.0
18-19	0.0	0.0	0.0	8.30176931458517E-4	0.0
20-21	0.0	0.0	0.0	0.001141493280755461	0.0
22-23	0.0	0.0	0.0	0.0020754423286462927	0.0
24-25	0.0	0.0	0.0	0.0037357961915633273	0.0
26-27	0.0	0.0	0.0	0.005188605821615733	0.0
28-29	0.0	0.0	0.0	0.009598920769989104	0.0
30-31	0.0	0.0	0.0	0.02397135889586468	0.0
32-33	0.0	0.0	0.0	0.05889067607533856	0.0
34-35	0.0	0.0	0.0	0.1038240024905308	0.0
36-37	0.0	0.0	0.0	0.17584185129455715	0.0
38-39	0.0	0.0	0.0	0.30213251699268406	0.0
40-41	0.0	0.0	0.0	0.45675297047683283	0.0
42-43	0.0	0.0	0.0	0.6030716546463966	0.0
44-45	0.0	0.0	0.0	0.7599750946920563	0.0
46-47	0.0	0.0	0.0	0.9348829969387226	0.0
48-49	0.0	0.0	0.0	1.1248378560680745	0.0
50-51	0.0	0.0	0.0	1.3276604576350335	0.0
52-53	0.0	0.0	0.0	1.5328179318217194	0.0
54-55	0.0	0.0	0.0	1.7184662481191304	0.0
56-57	0.0	0.0	0.0	1.935661287811965	0.0
58-59	0.0	0.0	0.0	2.1714834224043997	0.0
60-61	0.0	0.0	0.0	2.4291495875058375	0.0
62-63	0.0	0.0	0.0	2.6904477766824053	0.0
64-65	0.0	0.0	0.0	2.945208322523738	0.0
66-67	0.0	0.0	0.0	3.2194261401961293	0.0
68-69	0.0	0.0	0.0	3.532869817879936	0.0
70-71	0.0	0.0	0.0	3.8751621439319255	0.0
72-73	0.0	0.0	0.0	4.236548539407462	0.0
74-75	0.0	0.0	0.0	4.585222850620038	0.0
76-77	0.0	0.0	0.0	4.942406475380066	0.0
78-79	0.0	0.0	0.0	5.351009183832304	0.0
80-81	0.0	0.0	0.0	5.802002801847143	0.0
82-83	0.0	0.0	0.0	6.28018471436725	0.0
84-85	0.0	0.0	0.0	6.740102734395268	0.0
86-87	0.0	0.0	0.0	7.206817828049603	0.0
88-89	0.0	0.0	0.0	7.729258548228091	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1275	0.0	39.551003	1
GGTATCA	710	0.0	38.19252	1
CCGTGCG	100	3.0249794E-8	38.00714	9
ATAACCG	100	3.0388037E-8	37.987423	5
TAACCGT	120	3.805326E-9	35.61321	6
ATCAACG	1455	0.0	33.6143	3
AACCGTG	250	0.0	32.28931	7
TCAACGC	1515	0.0	32.28304	4
TCGCACC	1080	0.0	31.274935	1
CGCAGAG	1675	0.0	29.484304	8
CTGTGCG	1240	0.0	29.118376	9
CGCACCA	1180	0.0	28.571049	2
ACGCAGA	1790	0.0	27.58863	7
GTACATG	3235	0.0	26.911543	1
ACATGGG	3100	0.0	26.039764	3
TACATGG	3300	0.0	25.468842	2
GCACCAC	1410	0.0	25.257595	3
CGTTATA	95	0.001215459	25.038494	1
AAACCGT	190	1.1947122E-8	24.991724	6
ACACGCT	575	0.0	24.774406	6
>>END_MODULE