FastQCFastQC Report
Fri 10 Feb 2017
SRR3224508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963650
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT63900.6631038240024905No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT25700.2666943392310486TruSeq Adapter, Index 11 (95% over 21bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT16880.1751673325377471No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG15960.16562029782597415No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA15040.15607326311420122No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA14870.15430913713485186No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14110.14642245628599596No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG13780.14299797644372955No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG13130.1362527888756291No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG12270.12732838686245004TruSeq Adapter, Index 11 (96% over 28bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT11910.12359259067088674No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG11270.11695117521921859No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC11020.11435687230841073No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT10820.11228142997976444No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG10760.11165879728117056No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC10250.1063664193431225No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG10160.10543247029523167No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG9940.10314948373372076No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA9820.10190421833653299No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA9780.10148912987080372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1400.050.881749
GGTATCA7100.045.7011761
ACCGTGC1900.039.991268
GTATCAA13500.034.992761
TGCGCGG1100.034.53790712-13
AACCGTG2450.032.9519847
ATCAACG14800.031.7667063
ACCCCGT753.0820284E-431.6597526
AAACCGT2350.030.3125256
TCAACGC15800.029.7561574
AATGCGG1652.6520866E-928.781597
GTACATG31150.028.339391
CGCAGAG17550.027.330218
AATGCCG1754.971298E-927.1369299
ACATGGG30650.026.8049093
TACATGG32700.026.4315322
ACGCAGA18750.025.8343547
AGACCGC8700.025.1094556
GACCGCA8600.024.8492227
AAGACCG8600.024.2970165
Produced by FastQC (version 0.11.5)