##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963650
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.177244850308725	34.0	33.0	34.0	31.0	34.0
2	33.31353914803093	34.0	34.0	34.0	31.0	34.0
3	33.42951486535568	34.0	34.0	34.0	31.0	34.0
4	36.62959373216417	37.0	37.0	37.0	35.0	37.0
5	36.66804856535049	37.0	37.0	37.0	35.0	37.0
6	36.70786488870441	37.0	37.0	37.0	36.0	37.0
7	36.69462875525346	37.0	37.0	37.0	36.0	37.0
8	36.67933170757018	37.0	37.0	37.0	36.0	37.0
9	38.545385772842835	39.0	39.0	39.0	38.0	39.0
10-11	38.567218388419036	39.0	39.0	39.0	37.5	39.0
12-13	38.55765475016863	39.0	39.0	39.0	37.5	39.0
14-15	40.17399730192497	41.0	40.0	41.0	38.0	41.0
16-17	40.17993151040316	41.0	40.0	41.0	38.0	41.0
18-19	40.178573133399055	41.0	40.0	41.0	38.5	41.0
20-21	40.188410730036836	41.0	40.0	41.0	39.0	41.0
22-23	40.11990919939812	41.0	40.0	41.0	38.0	41.0
24-25	40.09063352877082	41.0	40.0	41.0	38.0	41.0
26-27	40.033055051107766	41.0	40.0	41.0	38.0	41.0
28-29	40.0018206817828	41.0	40.0	41.0	38.0	41.0
30-31	39.95156021377056	41.0	40.0	41.0	38.0	41.0
32-33	39.92070046178592	41.0	40.0	41.0	38.0	41.0
34-35	39.848041301302345	41.0	40.0	41.0	38.0	41.0
36-37	39.738515539874435	41.0	40.0	41.0	37.0	41.0
38-39	39.62875473460281	41.0	40.0	41.0	37.0	41.0
40-41	39.529654957712864	41.0	40.0	41.0	37.0	41.0
42-43	39.45659212369636	41.0	39.5	41.0	36.0	41.0
44-45	39.25322108649406	41.0	39.0	41.0	35.0	41.0
46-47	39.068642141856486	41.0	39.0	41.0	35.0	41.0
48-49	38.9910937581072	41.0	39.0	41.0	35.0	41.0
50-51	38.83548695065636	40.0	38.0	41.0	35.0	41.0
52-53	38.62086753489338	40.0	37.5	41.0	35.0	41.0
54-55	38.4389181756862	40.0	37.0	41.0	35.0	41.0
56-57	38.23713796502879	40.0	36.5	41.0	35.0	41.0
58-59	37.99644943703627	40.0	36.0	41.0	34.5	41.0
60-61	37.75750324287864	40.0	35.0	41.0	34.0	41.0
62-63	37.56164790120894	39.0	35.0	41.0	34.0	41.0
64-65	37.32528615161106	39.0	35.0	41.0	34.0	41.0
66-67	37.05348207336689	39.0	35.0	41.0	33.0	41.0
68-69	36.77620297825975	38.0	35.0	41.0	33.0	41.0
70-71	36.44896954288383	37.0	35.0	40.0	33.0	41.0
72-73	36.140875317802106	37.0	35.0	40.0	33.0	41.0
74-75	35.82930887770456	36.0	35.0	39.0	33.0	41.0
76-77	33.93203289576091	34.5	33.0	37.5	29.5	39.0
78-79	35.20507497535412	35.0	35.0	38.5	32.5	39.5
80-81	35.07520572822082	35.0	35.0	37.0	33.0	39.0
82-83	34.80876147979038	35.0	35.0	37.0	33.0	39.0
84-85	34.533607118767186	35.0	35.0	36.5	33.0	38.0
86-87	34.28931302858922	35.0	35.0	36.0	32.5	37.0
88-89	34.09182171950397	35.0	35.0	36.0	32.0	37.0
90-91	33.91099880662066	35.0	35.0	35.5	32.0	36.5
92-93	33.786822497794844	35.0	35.0	35.0	32.0	36.0
94-95	33.663659523685986	35.0	35.0	35.0	32.0	36.0
96-97	33.59909770144762	35.0	34.0	35.0	31.5	36.0
98-99	33.558701810823436	35.0	34.0	35.0	31.0	36.0
100-101	32.749602033933485	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	15.0
15	34.0
16	75.0
17	199.0
18	352.0
19	562.0
20	789.0
21	1049.0
22	1513.0
23	1561.0
24	1953.0
25	3144.0
26	4376.0
27	6419.0
28	9661.0
29	8221.0
30	6435.0
31	6695.0
32	8032.0
33	10879.0
34	18617.0
35	38995.0
36	109270.0
37	219147.0
38	335858.0
39	169657.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77169452507257	21.011618790244555	14.136055783538518	24.08063090114436
2	14.551548798837752	21.287604420692162	41.03045711617288	23.130389664297205
3	18.22362891091164	28.286203497120322	30.219374254137914	23.270793337830124
4	11.857624656254865	19.020598765111814	41.06843771078711	28.05333886784621
5	12.435531572666426	38.810356457219946	36.47081409225341	12.28329787786022
6	26.728791573704147	39.62559020391221	20.760026980750272	12.885591241633373
7	24.426918487002542	31.89186945467753	24.845431432574067	18.835780625745862
8	23.52980854044518	36.544596067036785	22.212732838686243	17.712862553831783
9	25.470969750428058	16.045555959113784	21.43776267316972	37.04571161728843
10-11	24.448451201162246	26.063664193431222	29.737923519950186	19.749961085456338
12-13	24.86353966689151	24.035853266227363	29.26856223732683	21.8320448295543
14-15	22.0407824417579	26.339282934675452	26.179473875369684	25.44046074819696
16-17	22.059253878482853	28.864058527473667	27.321693560940176	21.754994033103305
18-19	21.852487936491467	28.066621698749543	28.160846780470088	21.9200435842889
20-21	22.705487466033787	26.832333393694647	27.743771931781687	22.71840720848988
22-23	22.79162898381077	27.809973594760258	27.05559006033927	22.342807361089708
24-25	22.713169123995485	27.535261838347612	27.263793418343628	22.487775619313275
26-27	22.61745701229273	28.482589323471892	27.319577197822582	21.580376466412797
28-29	21.984602667992238	27.926019747646475	27.83635677767475	22.253020806686532
30-31	22.28720119961189	26.968748864986587	28.3905898956566	22.353460039744927
32-33	22.1225084898783	27.818782282940596	28.246999032322506	21.8117101948586
34-35	22.60075911469481	27.367771092933175	27.92436872322927	22.10710106914274
36-37	21.911797822186386	27.253698785941033	28.238986661851524	22.595516730021057
38-39	22.045555959113784	27.312821044985213	27.684221449696466	22.957401546204533
40-41	22.143776267316973	27.041249416281843	28.381103097597677	22.433871218803507
42-43	22.30384475691382	27.34727338764074	28.50433248586105	21.844549369584392
44-45	22.24111618890069	26.981276292349914	28.219802618299035	22.55780490045036
46-47	22.54565205312862	26.54333199883478	27.845221015288573	23.065794932748023
48-49	21.871734390711893	26.705432832351466	28.370783310382187	23.052049466554454
50-51	21.862917034192915	27.13490375136201	28.111606911222953	22.89057230322212
52-53	22.006549061666096	27.268368841575697	28.0943953169695	22.630686779788707
54-55	21.91698230685415	26.742281948840347	28.159238312665387	23.181497431640118
56-57	22.527671820588115	26.965489292169075	27.480397127143263	23.026441760099548
58-59	22.24585629480374	27.403136203367907	28.109459321279957	22.2415481805484
60-61	22.298775766106345	28.14263975303567	27.208946021187945	22.349638459670036
62-63	21.680900741970632	27.956415711098426	27.761220360089244	22.601463186841695
64-65	21.947802625434544	27.88647330462305	27.24101074041405	22.924713329528355
66-67	21.69065532091527	27.970061744409275	27.10361645825767	23.235666476417787
68-69	22.057541638561716	27.870388626576037	27.581902142894204	22.49016759196804
70-71	22.497898614642246	27.688527992528407	27.21657240699424	22.597000985835106
72-73	21.94422248741763	27.649561562808074	27.490582680433768	22.91563326934053
74-75	22.066102838167385	27.20764800498106	27.50863902869299	23.217610128158565
76-77	21.981021107778297	26.864280010522496	27.35283938818004	23.801859493519167
78-79	22.1947375822269	26.67651547347179	27.349586459084126	23.77916048521719
80-81	21.66407393993107	27.494180634354958	27.272769727467928	23.568975698246046
82-83	21.511104515744737	27.598183760628324	27.74139084169962	23.14932088192732
84-85	21.89875631145087	26.811660754100007	27.816615479768853	23.47296745468027
86-87	21.615939397084002	27.455559591137863	27.64650028537332	23.282000726404814
88-89	22.15031391065221	26.600010377211643	27.062470814092254	24.187204898043895
90-91	22.106055102993828	26.703626835469308	27.106418305401338	24.083899756135526
92-93	21.951030950568644	26.761753106290204	27.650561744700745	23.636654198440407
94-95	21.980335183936077	26.412909251284177	27.472785762465623	24.133969802314116
96-97	22.341825351528044	26.902402324495405	27.371192860478388	23.38457946349816
98-99	20.946194157629844	27.053805842370153	27.872567841021116	24.12743215897888
100-101	21.7082981349303	26.567520964575564	27.55138014950257	24.17280075099157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	2.5
4	2.0
5	1.0
6	1.5
7	4.0
8	8.5
9	11.0
10	12.5
11	12.0
12	15.5
13	17.5
14	13.0
15	15.5
16	23.0
17	27.5
18	31.0
19	41.0
20	50.5
21	61.0
22	76.5
23	92.0
24	133.5
25	210.0
26	319.0
27	450.5
28	738.5
29	1591.5
30	3480.5
31	6779.0
32	11430.5
33	17577.5
34	25038.0
35	33290.0
36	41197.5
37	46738.5
38	50343.0
39	52319.5
40	50944.0
41	47217.5
42	42688.0
43	37785.0
44	33197.5
45	29843.5
46	27604.0
47	25565.0
48	24415.5
49	26614.0
50	30298.0
51	29833.0
52	27893.5
53	28323.5
54	30891.5
55	31267.5
56	25440.0
57	21115.0
58	20874.5
59	20716.5
60	17333.0
61	12498.5
62	10041.5
63	6993.5
64	3751.5
65	2088.0
66	1632.0
67	1553.0
68	1183.0
69	684.0
70	365.0
71	210.0
72	147.5
73	130.0
74	113.0
75	85.0
76	56.0
77	36.5
78	27.5
79	16.0
80	8.0
81	5.5
82	4.0
83	2.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4684273335754683
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.820629896746743E-4
22-23	0.0016084678047008768
24-25	2.075442328646293E-4
26-27	0.003268821667617911
28-29	0.004306542831941058
30-31	5.188605821615732E-4
32-33	2.594302910807866E-4
34-35	0.0028537332018886523
36-37	0.0023867586779432364
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008872515954962901
46-47	0.0769470243345613
48-49	9.339490478908317E-4
50-51	0.0
52-53	5.188605821615732E-5
54-55	0.0
56-57	0.008249883256369012
58-59	0.036475898925958594
60-61	0.02822601566958958
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	5.188605821615732E-5
78-79	0.008457427489233643
80-81	0.0059150106366419345
82-83	0.0010896072225393036
84-85	0.0025424168525917086
86-87	0.0
88-89	0.0
90-91	0.0
92-93	5.188605821615732E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06812639443781456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	963650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.5001401995706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.41896431408946	24.907204736886314
2	15.857929535238519	11.893491616927118
3	6.6190809962005215	7.4464939604949905
4	3.2825732774334595	4.923878324764748
5	1.984291886565121	3.7205611971531236
6	1.2515137850070328	2.815916543967539
7	0.8255747037291925	2.1671416994544574
8	0.6154609148294189	1.8463896474767352
9	0.45167471075355303	1.5244078480072867
>10	2.33296146590703	15.648873120945042
>50	0.18789804896993553	4.886745591736067
>100	0.15403609759702688	11.892463822789026
>500	0.013322040871164186	3.393178127138916
>1k	0.0044406802903880615	2.2681900167087417
>5k	2.7754251814925384E-4	0.6650637455498949
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	6390	0.6631038240024905	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	2570	0.2666943392310486	TruSeq Adapter, Index 11 (95% over 21bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1688	0.1751673325377471	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1596	0.16562029782597415	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1504	0.15607326311420122	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1487	0.15430913713485186	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1411	0.14642245628599596	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1378	0.14299797644372955	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1313	0.1362527888756291	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG	1227	0.12732838686245004	TruSeq Adapter, Index 11 (96% over 28bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1191	0.12359259067088674	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1127	0.11695117521921859	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1102	0.11435687230841073	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1082	0.11228142997976444	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1076	0.11165879728117056	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1025	0.1063664193431225	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1016	0.10543247029523167	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	994	0.10314948373372076	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	982	0.10190421833653299	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	978	0.10148912987080372	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0377211643231463E-4	0.0
2	0.0	0.0	0.0	2.0754423286462926E-4	0.0
3	0.0	0.0	0.0	3.113163492969439E-4	0.0
4	0.0	0.0	0.0	3.113163492969439E-4	0.0
5	0.0	0.0	0.0	3.113163492969439E-4	0.0
6	0.0	0.0	0.0	3.113163492969439E-4	0.0
7	0.0	0.0	0.0	3.113163492969439E-4	0.0
8	0.0	0.0	0.0	3.113163492969439E-4	0.0
9	0.0	0.0	0.0	4.150884657292585E-4	0.0
10-11	0.0	0.0	0.0	4.150884657292585E-4	0.0
12-13	0.0	0.0	0.0	4.150884657292585E-4	0.0
14-15	0.0	0.0	0.0	6.226326985938878E-4	0.0
16-17	0.0	0.0	0.0	8.30176931458517E-4	0.0
18-19	0.0	0.0	0.0	8.30176931458517E-4	0.0
20-21	0.0	0.0	0.0	0.0010377211643231464	0.0
22-23	0.0	0.0	0.0	0.001971670212213978	0.0
24-25	0.0	0.0	0.0	0.003580138016914855	0.0
26-27	0.0	0.0	0.0	0.004981061588751102	0.0
28-29	0.0	0.0	0.0	0.009391376537124473	0.0
30-31	0.0	0.0	0.0	0.023919472837648524	0.0
32-33	0.0	0.0	0.0	0.05889067607533856	0.0
34-35	0.0	0.0	0.0	0.10444663518912467	0.0
36-37	0.0	0.0	0.0	0.17713900274996108	0.0
38-39	0.0	0.0	0.0	0.3045192756706273	0.0
40-41	0.0	0.0	0.0	0.46012556426088314	0.0
42-43	0.0	0.0	0.0	0.6074300835365538	0.0
44-45	0.0	0.0	0.0	0.7652674726301043	0.0
46-47	0.0	0.0	0.0	0.9410574378664452	0.0
48-49	0.0	0.0	0.0	1.1331396253826598	0.0
50-51	0.0	0.0	0.0	1.3381414413946973	0.0
52-53	0.0	0.0	0.0	1.544232864629274	0.0
54-55	0.0	0.0	0.0	1.7320603953717635	0.0
56-57	0.0	0.0	0.0	1.9516940798007576	0.0
58-59	0.0	0.0	0.0	2.1889171379650287	0.0
60-61	0.0	0.0	0.0	2.4499040107923005	0.0
62-63	0.0	0.0	0.0	2.7142115913454052	0.0
64-65	0.0	0.0	0.0	2.971618326155762	0.0
66-67	0.0	0.0	0.0	3.2488974212629067	0.0
68-69	0.0	0.0	0.0	3.5657655787889793	0.0
70-71	0.0	0.0	0.0	3.9113786125668035	0.0
72-73	0.0	0.0	0.0	4.277175322990713	0.0
74-75	0.0	0.0	0.0	4.629377886161988	0.0
76-77	0.0	0.0	0.0	4.990037876822498	0.0
78-79	0.0	0.0	0.0	5.401131116069113	0.0
80-81	0.0	0.0	0.0	5.8563793908576764	0.0
82-83	0.0	0.0	0.0	6.338245213511129	0.0
84-85	0.0	0.0	0.0	6.803196181186115	0.0
86-87	0.0	0.0	0.0	7.274321589788824	0.0
88-89	0.0	0.0	0.0	7.798993410470606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	140	0.0	50.88174	9
GGTATCA	710	0.0	45.701176	1
ACCGTGC	190	0.0	39.99126	8
GTATCAA	1350	0.0	34.99276	1
TGCGCGG	110	0.0	34.537907	12-13
AACCGTG	245	0.0	32.951984	7
ATCAACG	1480	0.0	31.766706	3
ACCCCGT	75	3.0820284E-4	31.659752	6
AAACCGT	235	0.0	30.312525	6
TCAACGC	1580	0.0	29.756157	4
AATGCGG	165	2.6520866E-9	28.78159	7
GTACATG	3115	0.0	28.33939	1
CGCAGAG	1755	0.0	27.33021	8
AATGCCG	175	4.971298E-9	27.136929	9
ACATGGG	3065	0.0	26.804909	3
TACATGG	3270	0.0	26.431532	2
ACGCAGA	1875	0.0	25.834354	7
AGACCGC	870	0.0	25.109455	6
GACCGCA	860	0.0	24.849222	7
AAGACCG	860	0.0	24.297016	5
>>END_MODULE