FastQCFastQC Report
Fri 10 Feb 2017
SRR3224507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373889
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG37180.27061865987718076No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24970.1817468514559764No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA23240.16915485894420873No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA17010.12380912868506845No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16200.11791345589054138No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC15200.11063484750223634No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14750.10735947372749909No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC14610.10634046855313639No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA14470.1053214633787737No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA14390.10473917470770928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.043.3333241
GTATCAA18100.043.1043241
AAACCGT2000.035.6105236
ATCAACG24450.030.6828213
TCAACGC25150.029.8288254
CCGTGCG2101.8189894E-1229.4099359
TCGCACC12700.028.8431431
GTACATG55150.028.55211
TAGACCG1004.9675495E-528.4884175
AACCGTG3150.027.1318267
CGCACCA13700.027.0328032
ACATGGG58450.026.4007263
ACCGTGC2950.025.753188
CGCAGAG30250.025.4286028
TACATGG61400.025.286952
TACCCCG950.001229587224.989845
ACGCAGA31300.024.7263687
TATCAAC30950.024.3923442
CTGTGCG16650.023.968189
CATGGGG40950.023.885284
Produced by FastQC (version 0.11.5)