##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224507_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1373889
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31504946906191	34.0	31.0	34.0	31.0	34.0
2	32.46915944446749	34.0	31.0	34.0	31.0	34.0
3	32.61129101404844	34.0	31.0	34.0	31.0	34.0
4	35.87400728879844	37.0	37.0	37.0	35.0	37.0
5	35.87777032933519	37.0	37.0	37.0	35.0	37.0
6	35.97903469639833	37.0	37.0	37.0	35.0	37.0
7	35.944379786139926	37.0	37.0	37.0	35.0	37.0
8	35.98113894208338	37.0	37.0	37.0	35.0	37.0
9	37.722885909997096	39.0	39.0	39.0	35.0	39.0
10-11	37.8042279980406	39.0	39.0	39.0	36.0	39.0
12-13	37.806629574878315	39.0	39.0	39.0	37.0	39.0
14-15	39.333480361222776	41.0	40.0	41.0	37.0	41.0
16-17	39.1729935970082	41.0	40.0	41.0	36.5	41.0
18-19	39.25247745633017	41.0	40.0	41.0	36.5	41.0
20-21	39.310611337597145	41.0	40.0	41.0	37.0	41.0
22-23	39.27055133274959	41.0	40.0	41.0	37.0	41.0
24-25	39.21692545758791	41.0	40.0	41.0	36.0	41.0
26-27	39.0627587090369	41.0	40.0	41.0	36.0	41.0
28-29	38.992983785444096	41.0	39.0	41.0	35.5	41.0
30-31	38.93226235889507	41.0	39.0	41.0	35.0	41.0
32-33	38.75743127719925	41.0	39.0	41.0	35.0	41.0
34-35	38.760559987014965	41.0	39.0	41.0	35.0	41.0
36-37	38.76710090844311	41.0	39.0	41.0	35.0	41.0
38-39	38.65886545419608	41.0	39.0	41.0	35.0	41.0
40-41	38.56950525115202	41.0	39.0	41.0	35.0	41.0
42-43	38.43093110142085	40.0	38.0	41.0	34.5	41.0
44-45	38.27258606772454	40.0	38.0	41.0	34.0	41.0
46-47	38.11691992584554	40.0	38.0	41.0	34.0	41.0
48-49	37.91906478616541	40.0	37.5	41.0	33.0	41.0
50-51	36.63072380665396	38.5	35.5	40.0	31.5	40.5
52-53	36.94908395074129	39.0	36.0	40.0	32.5	40.5
54-55	37.40077327935517	40.0	36.0	41.0	33.0	41.0
56-57	37.25071166593517	39.5	35.0	41.0	33.0	41.0
58-59	37.1042365867985	39.0	35.0	41.0	33.0	41.0
60-61	36.86186657000675	39.0	35.0	41.0	32.5	41.0
62-63	36.58495154994327	38.5	35.0	41.0	32.0	41.0
64-65	36.34734065124621	37.5	35.0	41.0	32.0	41.0
66-67	36.11500710756109	37.0	35.0	40.5	31.0	41.0
68-69	35.820723144300594	36.5	35.0	40.0	31.0	41.0
70-71	35.533878646673784	36.0	35.0	39.5	31.0	41.0
72-73	35.20991506591872	35.5	35.0	39.0	30.5	41.0
74-75	34.92345269523229	35.0	35.0	39.0	30.0	40.5
76-77	34.58305838390147	35.0	34.5	37.5	30.0	39.5
78-79	34.322977329318455	35.0	34.0	37.0	30.0	39.0
80-81	34.00898835349872	35.0	34.0	36.5	30.0	39.0
82-83	33.74373912302959	35.0	34.0	36.0	29.0	37.5
84-85	33.510513585886486	35.0	34.0	36.0	29.0	37.0
86-87	33.28509508410068	35.0	34.0	35.5	29.0	37.0
88-89	33.13737754651213	35.0	34.0	35.0	29.0	36.0
90-91	32.96648273623269	35.0	34.0	35.0	29.0	36.0
92-93	32.82729208837104	35.0	34.0	35.0	28.5	36.0
94-95	32.70041757376324	35.0	33.5	35.0	27.0	36.0
96-97	32.572346819866816	35.0	33.0	35.0	27.0	35.0
98-99	32.52447140926232	35.0	33.0	35.0	27.0	35.0
100-101	31.309339036850865	34.5	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	561.0
8	2243.0
9	1442.0
10	1045.0
11	1475.0
12	2027.0
13	2298.0
14	2258.0
15	2354.0
16	2412.0
17	2712.0
18	2834.0
19	3158.0
20	3587.0
21	4554.0
22	5543.0
23	5735.0
24	5935.0
25	6380.0
26	6675.0
27	7287.0
28	8173.0
29	9519.0
30	11774.0
31	15026.0
32	19923.0
33	28434.0
34	47314.0
35	86003.0
36	188324.0
37	332164.0
38	420511.0
39	134187.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24593614747801	18.420174143241784	13.192451168899701	25.141438540380502
2	16.28611648111054	19.471674546979084	37.80373654912191	26.438472422788468
3	20.073878090233173	25.296731531387273	27.877806366617293	26.751584011762265
4	13.506331297506566	16.298842191763672	37.25548424945538	32.93934226127438
5	14.281284732609404	37.89192576692877	33.718517289242435	14.108272211219392
6	30.90693644100797	36.13952801136045	18.5806859214973	14.372849626134279
7	27.991271492820747	30.518331539156367	22.04603137516932	19.444365592853572
8	26.214272040899957	34.530082124538445	20.292469042258872	18.963176792302725
9	26.69356840326984	15.719974466641773	19.91951314844212	37.66694398164626
10-11	26.34630599706381	25.094785677736702	27.031659762906614	21.52724856229288
12-13	26.42789919709671	22.589597849607937	27.433584518108812	23.54891843518654
14-15	23.613813300402224	24.46165350684301	24.606042645401246	27.31849054735352
16-17	23.674577313182134	27.333085916970806	25.68757286369386	23.3047639061532
18-19	23.72386835050811	26.89767066659095	26.171656015248345	23.206804967652598
20-21	24.605646304620254	25.495058336603986	26.009431138923617	23.88986421985214
22-23	24.603038470516815	25.982247850539036	25.486348660431236	23.928365018512913
24-25	23.97665182411264	25.97535526944641	25.85032255528284	24.19767035115811
26-27	23.778230992460088	26.880956176226757	26.14250496219127	23.198307869121887
28-29	23.612715437710033	26.70011915081932	26.275739888739192	23.41142552273146
30-31	23.620394369559694	26.186067433395277	26.266277697834393	23.927260499210632
32-33	23.471182897599444	26.312642433267897	26.485291024238496	23.730883644894167
34-35	24.055691544222277	25.87621707430513	26.08642328455938	23.981668096913214
36-37	23.47215240236332	25.97416448834774	26.682790048883405	23.870893060405532
38-39	23.58232161145658	26.395877750465313	25.95109378059459	24.070706857483515
40-41	23.85447883145158	26.07371341098131	26.597768099885936	23.47403965768117
42-43	23.830544079240436	26.31183016412344	26.506539315963096	23.35108644067303
44-45	23.829925233141424	25.94856220659413	26.223578457885317	23.99793410237913
46-47	23.83027304243647	25.626378841376557	26.39816608183048	24.145182034356488
48-49	23.35345140691861	26.107604034969345	26.491950950913797	24.046993607198253
50-51	23.40498395430781	26.447842584080668	26.237490801658648	23.90968265995288
52-53	23.631348675184093	26.238910130294368	25.96268694195819	24.167054252563343
54-55	23.71003043186167	25.73188226996504	26.30412646145358	24.253960836719706
56-57	23.62243237990842	26.015493245815346	26.25998170157851	24.102092672697722
58-59	23.769225309433985	26.116027961439276	26.36179161411612	23.752955115010618
60-61	23.555563806131367	26.35247260657166	26.257469652744188	23.834493934552793
62-63	23.25806169774367	26.045745447661762	26.641608480709444	24.054584373885124
64-65	23.925272846303805	25.494152747323966	26.130108381171404	24.450466025200825
66-67	23.32646111152364	26.140250108106457	25.848581075473593	24.684707704896315
68-69	23.47205633060604	25.978044805657518	26.863523909136767	23.686374954599682
70-71	23.743330065238165	25.779775513160086	26.528162027645614	23.948732393956135
72-73	23.60638304841221	25.96876457996243	26.692476612011596	23.73237575961377
74-75	23.59113436383871	25.844482341732117	26.58915676593961	23.975226528489564
76-77	23.594045807194032	25.50544476300487	26.315044374036038	24.585465055765056
78-79	23.784912561169328	24.917664376411388	26.686897497608552	24.610525564810732
80-81	23.478338765832753	25.97203261174738	26.127451498055258	24.422177124364605
82-83	23.373290508116753	26.24196344466506	26.425005926032195	23.959740121185995
84-85	23.530769412788295	25.35227059682231	26.824622810993226	24.29233717939617
86-87	23.412939156932307	26.17222008991615	26.413397258817366	24.001443494334175
88-89	23.72578879481232	25.509853768926	26.059326121834836	24.705031314426844
90-91	23.39362932522205	25.45624137029993	26.341938832030827	24.80819047244719
92-93	23.412113362009514	25.712734282905696	26.65625308213108	24.218899272953713
94-95	23.586002944924953	25.306738754004144	26.674170911915006	24.43308738915589
96-97	23.966928914926896	25.58594617177953	26.623548190574347	23.82357672271923
98-99	23.090089050750084	25.77653280364108	26.63839878242597	24.49497936318287
100-101	23.730727247174034	25.337066092261622	26.235884731588712	24.69632192897563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	1.0
3	0.5
4	1.0
5	1.5
6	4.0
7	7.5
8	10.5
9	13.5
10	14.0
11	15.5
12	18.5
13	22.5
14	30.5
15	35.0
16	37.0
17	38.5
18	39.0
19	48.5
20	68.5
21	83.5
22	98.0
23	109.0
24	136.0
25	183.5
26	235.5
27	298.0
28	361.5
29	442.5
30	586.0
31	1035.0
32	2161.0
33	4337.5
34	8769.0
35	16507.0
36	29145.0
37	43774.5
38	56191.5
39	64559.5
40	65922.5
41	62922.5
42	59552.5
43	57093.5
44	54606.5
45	51351.0
46	48402.0
47	44318.5
48	42443.5
49	44959.0
50	48207.0
51	48795.5
52	48466.0
53	50646.5
54	54563.5
55	55628.0
56	48509.5
57	41791.5
58	39691.5
59	38312.5
60	33503.5
61	26329.5
62	21547.5
63	15818.0
64	10278.0
65	7067.0
66	5534.0
67	4908.5
68	3757.0
69	2455.0
70	1698.5
71	1189.5
72	950.5
73	810.0
74	643.0
75	480.5
76	351.0
77	241.0
78	178.0
79	132.5
80	85.0
81	59.0
82	45.0
83	39.5
84	32.5
85	25.5
86	21.5
87	17.5
88	14.0
89	12.0
90	11.0
91	8.5
92	6.5
93	5.0
94	5.0
95	4.5
96	5.0
97	5.5
98	4.5
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13938535063604118
2	7.278608388305024E-5
3	2.9114433553220094E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03763040536753697
16-17	0.07529720377701546
18-19	0.026421348449547234
20-21	0.04119692347780643
22-23	0.06758187888541213
24-25	0.01808734184493798
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.022891223381219298
38-39	0.0055317423751118176
40-41	0.005968458878410119
42-43	0.0032753737747372603
44-45	0.011063484750223635
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015394256741265124
60-61	0.018014555761054932
62-63	0.030497369146998046
64-65	0.04949453704047416
66-67	0.017687018383581207
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017687018383581207
80-81	0.060084912245457964
82-83	0.05171451259890719
84-85	0.02958754309845992
86-87	0.060558021790697796
88-89	0.034973713305805634
90-91	0.0
92-93	0.003093408565029635
94-95	0.0
96-97	0.0
98-99	6.914677968889772E-4
100-101	0.04672866585291825
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1373889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.82943993324201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.87695416460825	26.636716288174426
2	15.826545221956104	12.60724864537278
3	6.542005499014205	7.816932451977755
4	3.3671204731937308	5.364420905402363
5	1.9431252776469419	3.8696795764401535
6	1.224308963010442	2.9258124181172525
7	0.8344788876625464	2.3265778712199796
8	0.6133877499994811	1.9544712435512634
9	0.42090513154446063	1.5087974088999079
>10	2.057603723873296	14.346870664114089
>50	0.14192960496437035	3.9781106790647045
>100	0.13536225598238633	11.07019614420588
>500	0.012944468841544272	3.4916673930443767
>1k	0.0033285777021113843	2.1024983104150183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3718	0.27061865987718076	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2497	0.1817468514559764	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2324	0.16915485894420873	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1701	0.12380912868506845	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1620	0.11791345589054138	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1520	0.11063484750223634	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1475	0.10735947372749909	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1461	0.10634046855313639	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1447	0.1053214633787737	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1439	0.10473917470770928	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4557216776610047E-4	0.0
8	0.0	0.0	0.0	1.4557216776610047E-4	0.0
9	0.0	0.0	0.0	2.183582516491507E-4	0.0
10-11	0.0	0.0	0.0	2.183582516491507E-4	0.0
12-13	0.0	3.639304194152512E-5	0.0	2.9114433553220094E-4	0.0
14-15	0.0	7.278608388305024E-5	0.0	3.6393041941525117E-4	0.0
16-17	0.0	7.278608388305024E-5	0.0	3.6393041941525117E-4	0.0
18-19	0.0	7.278608388305024E-5	0.0	5.095025871813516E-4	0.0
20-21	0.0	7.278608388305024E-5	0.0	6.914677968889772E-4	0.0
22-23	0.0	7.278608388305024E-5	0.0	0.0010553982163042284	0.0
24-25	0.0	7.278608388305024E-5	0.0	0.001965224264842356	0.0
26-27	0.0	7.278608388305024E-5	0.0	0.0032753737747372603	0.0
28-29	0.0	7.278608388305024E-5	0.0	0.005495349333170293	0.0
30-31	0.0	7.278608388305024E-5	0.0	0.011900524714878714	0.0
32-33	0.0	7.278608388305024E-5	0.0	0.030133438727582798	0.0
34-35	0.0	7.278608388305024E-5	0.0	0.05557217504470885	0.0
36-37	0.0	7.278608388305024E-5	0.0	0.0972422080677551	0.0
38-39	0.0	1.0917912582457536E-4	0.0	0.17727050729716884	0.0
40-41	0.0	1.4557216776610047E-4	0.0	0.27276584935173076	0.0
42-43	0.0	1.4557216776610047E-4	0.0	0.36221994644399946	0.0
44-45	0.0	1.4557216776610047E-4	0.0	0.46648601160646896	0.0
46-47	0.0	1.4557216776610047E-4	0.0	0.5860007613424374	0.0
48-49	0.0	1.4557216776610047E-4	0.0	0.7106833230341025	0.0
50-51	0.0	1.4557216776610047E-4	0.0	0.8372219298647854	0.0
52-53	0.0	1.8196520970762558E-4	0.0	0.9714394685451299	0.0
54-55	0.0	2.183582516491507E-4	0.0	1.1062029028545974	0.0
56-57	0.0	2.183582516491507E-4	0.0	1.2589444998831785	0.0
58-59	0.0	2.183582516491507E-4	0.0	1.426570851065843	0.0
60-61	0.0	2.183582516491507E-4	0.0	1.6159602413295397	0.0
62-63	0.0	2.183582516491507E-4	0.0	1.8084066471163245	0.0
64-65	0.0	2.183582516491507E-4	0.0	1.9988150425543838	0.0
66-67	0.0	2.183582516491507E-4	0.0	2.2109864770734755	0.0
68-69	0.0	2.183582516491507E-4	0.0	2.4600240630793317	0.0
70-71	0.0	2.183582516491507E-4	0.0	2.730679115998454	0.0
72-73	0.0	2.183582516491507E-4	0.0	3.01192454412256	3.639304194152512E-5
74-75	0.0	2.183582516491507E-4	0.0	3.2962997738536375	7.278608388305024E-5
76-77	0.0	2.183582516491507E-4	0.0	3.586934606798657	7.278608388305024E-5
78-79	0.0	2.183582516491507E-4	0.0	3.9167647459146995	7.278608388305024E-5
80-81	0.0	2.183582516491507E-4	0.0	4.288483276305437	7.278608388305024E-5
82-83	0.0	2.183582516491507E-4	0.0	4.687169050774845	7.278608388305024E-5
84-85	0.0	2.183582516491507E-4	0.0	5.089348557270638	7.278608388305024E-5
86-87	0.0	2.183582516491507E-4	0.0	5.50459316582344	7.278608388305024E-5
88-89	0.0	2.183582516491507E-4	0.0	5.958559971002025	7.278608388305024E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1010	0.0	43.333324	1
GTATCAA	1810	0.0	43.104324	1
AAACCGT	200	0.0	35.610523	6
ATCAACG	2445	0.0	30.682821	3
TCAACGC	2515	0.0	29.828825	4
CCGTGCG	210	1.8189894E-12	29.409935	9
TCGCACC	1270	0.0	28.843143	1
GTACATG	5515	0.0	28.5521	1
TAGACCG	100	4.9675495E-5	28.488417	5
AACCGTG	315	0.0	27.131826	7
CGCACCA	1370	0.0	27.032803	2
ACATGGG	5845	0.0	26.400726	3
ACCGTGC	295	0.0	25.75318	8
CGCAGAG	3025	0.0	25.428602	8
TACATGG	6140	0.0	25.28695	2
TACCCCG	95	0.0012295872	24.98984	5
ACGCAGA	3130	0.0	24.726368	7
TATCAAC	3095	0.0	24.392344	2
CTGTGCG	1665	0.0	23.96818	9
CATGGGG	4095	0.0	23.88528	4
>>END_MODULE