FastQCFastQC Report
Fri 10 Feb 2017
SRR3224507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373889
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG30320.2206874063334083No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT28860.21006063808648298No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21590.15714515510350546No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT20100.14630002860493097No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA18460.13436311084811073No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA17440.12693893029203962No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA15470.1126000717670787No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG15450.11245449959931261No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC14870.10823290673409569No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG14510.10561260771430588No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG14410.10488474687547539No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT14330.10430245820441097No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG14120.10277395044286694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.050.1691131
GTATCAA20900.041.5425451
GCGTAAT550.002525767334.6948741
ATCAACG25800.032.579273
TCAACGC26250.032.0207674
GTACATG59200.028.4459861
ACCGTGC2950.027.366218
CATACCG700.00840211227.1362425
ACATGGG61300.026.8042353
CAACGCA31650.026.707555
TACATGG62750.026.336212
CGCAGAG32100.026.1852078
CATGGGG43700.025.9719314
ACGCAGA32800.025.6263777
AACGCAG33000.025.614976
ATTAACC5650.025.2150943
TATCAAC34050.024.9645482
TCGCACC12950.024.3132021
AAACCGT2151.6680133E-924.2964046
CGCACCA13300.023.9227432
Produced by FastQC (version 0.11.5)