FastQCFastQC Report
Fri 10 Feb 2017
SRR3224504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882216
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG17500.19836411944467114No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA14690.1665125094081268No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA11050.1252527725636352No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC9890.11210406521758842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1100.047.512119
GGTATCA7350.043.352161
GTATCAA13550.036.5020561
AACCGTG1451.6370905E-1136.0171137
ATCAACG13550.034.688073
TCAACGC14150.033.2171944
AAACCGT1605.2750693E-1132.6405076
TCGCACC7900.028.8959791
GTCTAAT6100.028.8466361
CGCACCA8400.027.6949772
CGCAGAG17500.027.1359258
ACGCAGA17700.026.8232217
TCTAATG6600.026.6159522
ATGCGGG1257.4030213E-626.5932088
ACCGTGC2007.203198E-1026.1183288
CAACGCA18250.026.014855
ACATGGG29650.025.7801483
GTTATTA6650.025.707728
CTAATGT6100.025.6843343
TACATGG31900.025.1524472
Produced by FastQC (version 0.11.5)