##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224504_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882216
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36684553442694	34.0	31.0	34.0	31.0	34.0
2	32.53124291556716	34.0	31.0	34.0	31.0	34.0
3	32.67529040507087	34.0	31.0	34.0	31.0	34.0
4	35.941916718808095	37.0	37.0	37.0	35.0	37.0
5	35.963829719705835	37.0	37.0	37.0	35.0	37.0
6	36.06636696681992	37.0	37.0	37.0	35.0	37.0
7	36.02574992972242	37.0	37.0	37.0	35.0	37.0
8	36.06139652874126	37.0	37.0	37.0	35.0	37.0
9	37.80845733924572	39.0	39.0	39.0	36.0	39.0
10-11	37.88874096593125	39.0	39.0	39.0	37.0	39.0
12-13	37.90565122373659	39.0	39.0	39.0	37.0	39.0
14-15	39.44104958422881	41.0	40.0	41.0	37.0	41.0
16-17	39.292249857177836	41.0	40.0	41.0	37.0	41.0
18-19	39.386546492015555	41.0	40.0	41.0	37.0	41.0
20-21	39.4511978925796	41.0	40.0	41.0	37.0	41.0
22-23	39.4138929695222	41.0	40.0	41.0	37.0	41.0
24-25	39.37930903542896	41.0	40.0	41.0	37.0	41.0
26-27	39.239952007218186	41.0	40.0	41.0	36.5	41.0
28-29	39.17733072172801	41.0	40.0	41.0	36.0	41.0
30-31	39.10388328935318	41.0	40.0	41.0	36.0	41.0
32-33	38.916564650833806	41.0	39.0	41.0	35.0	41.0
34-35	38.93044447164867	41.0	39.0	41.0	35.0	41.0
36-37	38.944601435476116	41.0	39.0	41.0	35.0	41.0
38-39	38.847236957842526	41.0	39.0	41.0	35.0	41.0
40-41	38.7794565049829	41.0	39.0	41.0	35.0	41.0
42-43	38.66415140963211	41.0	39.0	41.0	35.0	41.0
44-45	38.524015093809226	40.5	39.0	41.0	35.0	41.0
46-47	38.39077901556988	40.0	38.0	41.0	34.0	41.0
48-49	38.2318831215938	40.0	38.0	41.0	34.0	41.0
50-51	37.02012942408662	39.0	36.5	40.0	32.0	40.5
52-53	37.356907492042765	39.5	37.0	40.5	33.0	40.5
54-55	37.86628898138324	40.0	37.0	41.0	33.0	41.0
56-57	37.77407346953581	40.0	37.0	41.0	33.0	41.0
58-59	37.67531760929296	40.0	36.5	41.0	33.0	41.0
60-61	37.48287947622805	40.0	36.0	41.0	33.0	41.0
62-63	37.24320744579559	40.0	35.0	41.0	33.0	41.0
64-65	37.04383223609638	39.0	35.0	41.0	33.0	41.0
66-67	36.84655628553551	39.0	35.0	41.0	32.0	41.0
68-69	36.587407732346726	38.5	35.0	41.0	32.0	41.0
70-71	36.31153368336099	37.5	35.0	40.5	31.5	41.0
72-73	36.00781157902373	37.0	35.0	40.0	31.0	41.0
74-75	35.70898963519139	37.0	35.0	39.5	31.0	41.0
76-77	35.33484770169664	36.0	35.0	39.0	31.0	41.0
78-79	35.04911949001151	36.0	35.0	39.0	31.0	40.0
80-81	34.6752490319831	35.0	35.0	37.5	30.0	39.0
82-83	34.34881763649719	35.0	34.5	37.0	30.0	39.0
84-85	34.044402391251126	35.0	34.0	37.0	30.0	38.5
86-87	33.75507698794853	35.0	34.0	36.0	29.5	37.0
88-89	33.530775909754524	35.0	34.0	36.0	29.0	37.0
90-91	33.320005531525155	35.0	34.0	35.5	29.0	36.5
92-93	33.147652615685956	35.0	34.0	35.0	29.0	36.0
94-95	32.99145220671582	35.0	34.0	35.0	29.0	36.0
96-97	32.85109712360692	35.0	34.0	35.0	29.0	36.0
98-99	32.78343285544583	35.0	34.0	35.0	29.0	36.0
100-101	31.688785966248627	34.5	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	247.0
8	746.0
9	482.0
10	387.0
11	689.0
12	1139.0
13	1283.0
14	1325.0
15	1438.0
16	1497.0
17	1652.0
18	1951.0
19	2254.0
20	2603.0
21	3349.0
22	3888.0
23	3854.0
24	3448.0
25	3767.0
26	3990.0
27	4323.0
28	4769.0
29	5369.0
30	6809.0
31	8333.0
32	11308.0
33	15325.0
34	24856.0
35	44268.0
36	93428.0
37	175230.0
38	300272.0
39	147901.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51936583544006	21.3700197637208	13.97548208834758	23.135132312491557
2	14.50156707831991	21.098485063164873	41.371774454072984	23.028173404442228
3	18.56467769121516	27.35291817282221	30.03038948643922	24.052014649523414
4	11.850385846550052	17.670162409205908	41.42171531688385	29.057736427360194
5	12.34516263590776	39.49712995456895	35.997306782012565	12.160400627510723
6	27.494513815210787	39.975584210669496	19.888893422925904	12.64100855119381
7	25.29255873844954	32.43627410974183	24.563372235370927	17.707794916437695
8	23.80029380559863	37.27375155290768	21.899738839467886	17.026215802025806
9	25.226588499868512	16.823317645565258	22.01501673059659	35.93507712396964
10-11	23.790375599626394	27.170726896814383	29.63027195154021	19.408625552019007
12-13	24.188520725083198	24.723423742031432	29.97174161429854	21.116313918586833
14-15	21.525974541388955	26.83132592963844	26.896072352710394	24.746627176262216
16-17	21.184203287588257	29.815724683341493	27.866062227445116	21.134009801625133
18-19	21.246312299741717	29.149102139943267	28.923701736762265	20.680883823552755
20-21	22.23710231963205	27.734832128310032	28.756191430878232	21.271874121179682
22-23	22.02410354623041	28.267308792730034	28.199878750689756	21.508708910349803
24-25	21.41574028568303	28.334078746209872	28.434241830076473	21.815939138030625
26-27	21.449452288328484	28.83959257143375	28.62592607706049	21.08502906317727
28-29	21.25550885497429	28.83160133119327	28.693879956835964	21.219009856996472
30-31	21.525794136583333	28.203863906344935	28.638621380704954	21.631720576366785
32-33	21.333607642572794	28.223416940975905	28.83182803304406	21.61114738340724
34-35	21.677117621988266	28.166458100963936	28.518809452560372	21.63761482448743
36-37	21.511666137590133	28.005702689220442	28.783105074599792	21.699526098589633
38-39	21.44312577685605	28.437933416953697	28.356825819308597	21.762114986881652
40-41	21.740404469214212	27.996125456757976	28.96884212029788	21.29462795372993
42-43	21.798126368397757	28.04479304639984	28.587646319020664	21.569434266181737
44-45	21.85170217095452	27.969099564856847	28.339909321087127	21.839288943101504
46-47	21.804580737597142	27.885914560606473	28.310243749829972	21.999260951966413
48-49	21.301982734387042	28.211911822048112	28.53292164277229	21.95318380079255
50-51	21.47591972940867	28.304462852634728	28.25645873572912	21.963158682227483
52-53	21.703641738531154	28.285533248093436	27.937602582587484	22.073222430787926
54-55	21.704718572322424	27.98798706892643	28.065575777360646	22.241718581390497
56-57	21.70709894175576	28.189298312431422	28.03225060529394	22.071352140518876
58-59	21.790385710925122	28.150293768369377	28.351694446885034	21.707626073820464
60-61	21.881045953926463	28.420156987188317	28.025109787295793	21.673687271589422
62-63	21.55561388520234	28.135613783156955	28.354557828837006	21.954214502803698
64-65	22.099916478274995	27.698820546230944	27.945870210754855	22.255392764739206
66-67	21.522666193527247	28.206516286180566	27.782107960816347	22.488709559475836
68-69	21.5715879104437	28.038881634429664	28.53133472981673	21.8581957253099
70-71	21.952106967001278	27.63812943768873	28.448305176963462	21.961458418346528
72-73	21.740707491135957	27.956815564442266	28.479533356910324	21.82294358751145
74-75	21.800330077894756	27.78412542960001	28.192868866581428	22.22267562592381
76-77	21.54109651151192	27.72161239424359	28.179946861086176	22.557344233158318
78-79	21.68717246695973	27.226657823806	28.428833394269716	22.657336314964557
80-81	21.591119166083526	27.912067223230984	28.111847425313506	22.384966185371983
82-83	21.422726955705215	28.137511900569923	28.218483973710164	22.2212771700147
84-85	21.744567996562157	27.508109879369442	28.471204117244874	22.276118006823523
86-87	21.297969696574462	28.216868139439537	28.366462878332072	22.118699285653936
88-89	21.66937665689256	27.56644011601933	28.033941777163967	22.730241449924144
90-91	21.4079091741705	27.578846903706122	28.27142105788152	22.741822864241865
92-93	21.58491160614291	27.57841865906133	28.588690845942132	22.247978888853623
94-95	21.651500312848555	27.31048858782883	28.57809198654298	22.459919112779637
96-97	21.892030976540894	27.523588327575105	28.52923773769689	22.05514295818711
98-99	21.06676475372346	27.875011547710603	28.412638948004982	22.64558475056095
100-101	21.580121079337754	27.500046778333637	28.26981969645681	22.6500124458718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	1.0
4	3.0
5	6.5
6	12.5
7	18.5
8	19.5
9	26.0
10	38.0
11	41.5
12	46.5
13	57.0
14	62.5
15	74.0
16	79.0
17	96.5
18	119.0
19	129.0
20	142.5
21	159.0
22	200.0
23	276.0
24	371.0
25	481.5
26	771.0
27	1281.0
28	2333.5
29	4603.5
30	8457.0
31	13735.0
32	20766.5
33	28523.5
34	36124.0
35	42929.5
36	48879.0
37	51560.5
38	49156.0
39	44797.0
40	38390.5
41	31908.5
42	27475.0
43	24110.0
44	21997.5
45	21147.5
46	21458.0
47	21226.5
48	21380.5
49	23129.0
50	24924.0
51	24780.0
52	23808.5
53	24486.5
54	25595.0
55	25287.5
56	21512.0
57	18165.5
58	16962.0
59	15644.5
60	13311.0
61	10318.0
62	8418.5
63	6031.0
64	3662.5
65	2496.5
66	1948.0
67	1664.5
68	1325.5
69	891.5
70	566.5
71	415.0
72	336.5
73	273.5
74	213.0
75	162.5
76	116.5
77	79.5
78	58.5
79	41.0
80	25.0
81	17.0
82	12.5
83	10.0
84	7.0
85	7.5
86	6.0
87	3.5
88	4.0
89	4.5
90	3.0
91	2.0
92	3.0
93	2.5
94	1.0
95	1.0
96	2.5
97	3.5
98	2.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14837636134461402
2	1.1335092539695494E-4
3	3.400527761908648E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03559219057464385
16-17	0.07112770568658923
18-19	0.026070712841299637
20-21	0.03989952573972814
22-23	0.0636465446103902
24-25	0.01751271797382954
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.01994976286986407
38-39	0.006177625434134044
40-41	0.005384168956355359
42-43	0.002890448597622351
44-45	0.010484960599218333
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.016265857794463035
60-61	0.019212981854783862
62-63	0.028961161438921988
64-65	0.04641720395005305
66-67	0.018646227227799086
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016832612421447807
80-81	0.05633540992228661
82-83	0.0487975733833891
84-85	0.030264697080986967
86-87	0.05673213816117595
88-89	0.03332517206670475
90-91	0.0
92-93	0.0031738259111147383
94-95	0.0
96-97	0.0
98-99	7.367810150802071E-4
100-101	0.04505699284528959
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	882216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.41181088453747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53439711131323	35.84055912688151
2	14.395992754362604	14.226641429474737
3	5.374791537170921	7.967345489355061
4	2.5300285244409495	5.000531639286463
5	1.4141784241699566	3.4938558426039568
6	0.8543934038161475	2.533027517421585
7	0.5907160847550895	2.043184602646103
8	0.3924449161579832	1.5513131183837146
9	0.2982785363658519	1.3264634366843497
>10	1.4072498131793876	12.377972140774153
>50	0.1189538036465721	4.074165393162342
>100	0.08416952329366463	7.772344613298212
>500	0.0037099514338496294	1.2971857311526909
>1k	6.956158938468055E-4	0.49540991887513114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1750	0.19836411944467114	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1469	0.1665125094081268	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1105	0.1252527725636352	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	989	0.11210406521758842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1335092539695494E-4
2	0.0	0.0	0.0	0.0	1.1335092539695494E-4
3	0.0	0.0	0.0	0.0	1.1335092539695494E-4
4	0.0	0.0	0.0	0.0	1.1335092539695494E-4
5	0.0	0.0	0.0	0.0	1.1335092539695494E-4
6	0.0	0.0	0.0	0.0	1.1335092539695494E-4
7	0.0	0.0	0.0	0.0	1.1335092539695494E-4
8	0.0	0.0	0.0	0.0	1.1335092539695494E-4
9	0.0	0.0	0.0	0.0	1.1335092539695494E-4
10-11	0.0	0.0	0.0	0.0	1.1335092539695494E-4
12-13	0.0	0.0	0.0	0.0	3.400527761908648E-4
14-15	0.0	0.0	0.0	1.1335092539695494E-4	3.400527761908648E-4
16-17	0.0	0.0	0.0	2.8337731349238734E-4	3.400527761908648E-4
18-19	0.0	0.0	0.0	6.801055523817296E-4	4.5340370158781975E-4
20-21	0.0	0.0	0.0	6.801055523817296E-4	4.5340370158781975E-4
22-23	0.0	0.0	0.0	0.0010201583285725944	4.5340370158781975E-4
24-25	0.0	0.0	0.0	0.0015302374928588915	4.5340370158781975E-4
26-27	0.0	0.0	0.0	0.0018702902690497566	4.5340370158781975E-4
28-29	0.0	0.0	0.0	0.0034572032246071257	4.5340370158781975E-4
30-31	0.0	0.0	0.0	0.006631029135721864	4.5340370158781975E-4
32-33	0.0	0.0	0.0	0.0160958314063676	4.5340370158781975E-4
34-35	0.0	0.0	0.0	0.029357889677811333	6.801055523817296E-4
36-37	0.0	0.0	0.0	0.05367166317545817	6.801055523817296E-4
38-39	0.0	0.0	0.0	0.09170089864613655	6.801055523817296E-4
40-41	0.0	0.0	0.0	0.13732464611841091	6.801055523817296E-4
42-43	0.0	0.0	0.0	0.1843652801581472	6.801055523817296E-4
44-45	0.0	0.0	0.0	0.23435303825820433	6.801055523817296E-4
46-47	0.0	0.0	0.0	0.29221868567334985	6.801055523817296E-4
48-49	0.0	0.0	0.0	0.35484507195516746	6.801055523817296E-4
50-51	0.0	0.0	0.0	0.42421583829810383	7.934564777786846E-4
52-53	0.0	0.0	0.0	0.49925415091088804	7.934564777786846E-4
54-55	0.0	0.0	0.0	0.5822837037641575	7.934564777786846E-4
56-57	0.0	0.0	0.0	0.6784052885007754	7.934564777786846E-4
58-59	0.0	0.0	0.0	0.7815546306120043	7.934564777786846E-4
60-61	0.0	0.0	0.0	0.8944521523073714	7.934564777786846E-4
62-63	0.0	0.0	0.0	1.0108635526900442	7.934564777786846E-4
64-65	0.0	0.0	0.0	1.1333959030441525	7.934564777786846E-4
66-67	0.0	0.0	0.0	1.2720240848046283	8.50131940477162E-4
68-69	0.0	0.0	0.0	1.4308854067484607	9.068074031756395E-4
70-71	0.0	0.0	0.0	1.6095831406367602	9.068074031756395E-4
72-73	0.0	0.0	0.0	1.8019396610353926	9.068074031756395E-4
74-75	0.0	0.0	0.0	1.9968465772554567	9.068074031756395E-4
76-77	0.0	0.0	0.0	2.203995393418392	9.068074031756395E-4
78-79	0.0	0.0	0.0	2.4386318089900887	9.63482865874117E-4
80-81	0.0	0.0	0.0	2.702059359612612	0.0010201583285725944
82-83	0.0	0.0	0.0	2.9909341929867512	0.0011335092539695494
84-85	0.0	0.0	0.0	3.2917108735275713	0.0011335092539695494
86-87	0.0	0.0	0.0	3.5943011688747424	0.0012468601793665043
88-89	0.0	0.0	0.0	3.9396814385592647	0.0013602111047634593
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	110	0.0	47.51211	9
GGTATCA	735	0.0	43.35216	1
GTATCAA	1355	0.0	36.502056	1
AACCGTG	145	1.6370905E-11	36.017113	7
ATCAACG	1355	0.0	34.68807	3
TCAACGC	1415	0.0	33.217194	4
AAACCGT	160	5.2750693E-11	32.640507	6
TCGCACC	790	0.0	28.895979	1
GTCTAAT	610	0.0	28.846636	1
CGCACCA	840	0.0	27.694977	2
CGCAGAG	1750	0.0	27.135925	8
ACGCAGA	1770	0.0	26.823221	7
TCTAATG	660	0.0	26.615952	2
ATGCGGG	125	7.4030213E-6	26.593208	8
ACCGTGC	200	7.203198E-10	26.118328	8
CAACGCA	1825	0.0	26.01485	5
ACATGGG	2965	0.0	25.780148	3
GTTATTA	665	0.0	25.70772	8
CTAATGT	610	0.0	25.684334	3
TACATGG	3190	0.0	25.152447	2
>>END_MODULE