FastQCFastQC Report
Fri 10 Feb 2017
SRR3224504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882216
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA10530.11935852444299355No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG10120.1147111365017184No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT9710.11006374856044325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1550.042.888439
AACCGTG1900.042.4853487
GTATCAA13850.042.048361
TACCCCG500.001618351937.9868975
ATCACGC500.001618351937.9868973
GGTATCA8450.035.589581
ATCAACG16250.034.4804153
TCAACGC16650.033.3668674
AAACCGT2300.033.0320856
ACCGTGC2500.030.3895198
ACCCGCG2500.028.4901756
AGGTCGT1004.9634757E-528.4901736
CAACGCA19950.028.0855485
AACGCAG20800.026.9378226
TATCAAC21200.026.8775222
ACATGGG31250.026.7427753
CGCAGAG20850.026.6454858
TAGGTCG908.9639216E-426.379795
TACATGG33800.025.9895552
GTACATG34950.025.9504811
Produced by FastQC (version 0.11.5)