FastQCFastQC Report
Fri 10 Feb 2017
SRR3224502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340224
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG12970.381219431903687No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9690.28481235891647855No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA8810.2589470466516178No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC8780.25806527464258844No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT7580.22279439428141457No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA6010.17664832580887885No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT5580.16400959367945825No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA5160.1516647855530474No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA4930.1449045334838224No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC4890.1437288374717833No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC4870.14314098946576373No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA4820.14167136945071482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.13990782543265612No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG4760.13990782543265612No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG4610.1354989653875094No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC4550.1337354213694507No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA4520.13285364936042138No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA4040.11874529721595184No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC4030.11845137321294205No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC3940.11580605718585402No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG3840.1128668171557562No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC3770.11080934913468773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.10698833709556056No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG3630.10669441309255079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT1250.064.5737156
CCGTGCG1400.061.0914769
TACGAGT250.00502633656.9768074
GGTATCA4650.055.276961
ACCGTGC1550.055.1388478
AACCGTG1450.052.3924677
GTATCAA8600.047.0461161
GACCGTG702.0552124E-433.9147647
ATCAACG12200.033.859173
CTAATGT4100.033.583893
ATACAAA2850.033.403231
ATGTTAT3900.032.8712356
TGGTATC2600.032.8712352
GTCTAAT3650.032.6024671
AATGCGG1051.9025047E-631.6537847
TCAACGC13000.031.410294
GTTATTA4000.030.8624368
TCTAATG4200.030.523292
AAATGCG1102.7346578E-630.2149736
TATATGA804.4977714E-429.675422
Produced by FastQC (version 0.11.5)