##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224502_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340224
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.062473546839726	34.0	31.0	34.0	30.0	34.0
2	32.229707486832204	34.0	31.0	34.0	30.0	34.0
3	32.37182268152746	34.0	31.0	34.0	31.0	34.0
4	35.66409777088036	37.0	35.0	37.0	35.0	37.0
5	35.670572916666664	37.0	35.0	37.0	35.0	37.0
6	35.78658472065463	37.0	37.0	37.0	35.0	37.0
7	35.74543536023326	37.0	37.0	37.0	35.0	37.0
8	35.78307526805869	37.0	37.0	37.0	35.0	37.0
9	37.48207357505643	39.0	38.0	39.0	35.0	39.0
10-11	37.562250164597444	39.0	38.5	39.0	35.0	39.0
12-13	37.54585214446953	39.0	38.0	39.0	35.0	39.0
14-15	38.995460343773516	41.0	39.0	41.0	36.0	41.0
16-17	38.83908542607223	41.0	39.0	41.0	35.5	41.0
18-19	38.924749576749434	41.0	39.0	41.0	35.5	41.0
20-21	38.993432268152745	41.0	39.5	41.0	36.0	41.0
22-23	38.933484998118885	41.0	39.0	41.0	35.0	41.0
24-25	38.86354137274266	41.0	39.0	41.0	35.0	41.0
26-27	38.65758735421369	41.0	39.0	41.0	35.0	41.0
28-29	38.57283436794582	41.0	39.0	41.0	35.0	41.0
30-31	38.46891459744168	40.0	38.5	41.0	34.0	41.0
32-33	38.243242687170806	40.0	38.0	41.0	34.0	41.0
34-35	38.21307138826185	40.0	38.0	41.0	34.0	41.0
36-37	38.18506924849511	40.0	38.0	41.0	34.0	41.0
38-39	38.04053799849511	40.0	38.0	41.0	33.5	41.0
40-41	37.91302641788939	40.0	38.0	41.0	33.0	41.0
42-43	37.71147685054552	40.0	37.0	41.0	33.0	41.0
44-45	37.47607311653499	40.0	37.0	41.0	32.0	41.0
46-47	37.21272014907825	40.0	36.0	41.0	31.0	41.0
48-49	36.92534036399549	40.0	36.0	41.0	31.0	41.0
50-51	35.570507959462006	38.0	34.0	40.0	28.5	40.5
52-53	35.88192337989089	38.5	35.0	40.0	29.5	40.5
54-55	36.2810839329383	39.0	35.0	41.0	30.0	41.0
56-57	36.087564369356656	38.0	35.0	41.0	30.0	41.0
58-59	35.89527634734763	37.5	35.0	41.0	30.0	41.0
60-61	35.60679875611362	37.0	35.0	40.0	29.0	41.0
62-63	35.294795487678705	36.0	35.0	40.0	29.0	41.0
64-65	35.02051442579007	36.0	35.0	40.0	28.5	41.0
66-67	34.755802059819416	35.0	34.5	39.0	28.0	41.0
68-69	34.439137156696766	35.0	34.0	39.0	28.0	41.0
70-71	34.160635346124906	35.0	34.0	38.5	27.0	41.0
72-73	33.87159782966516	35.0	34.0	37.0	26.5	40.0
74-75	33.615472453442436	35.0	34.0	37.0	26.0	39.5
76-77	33.31976433173439	35.0	34.0	36.0	26.0	39.0
78-79	33.124201996331834	35.0	34.0	36.0	26.0	39.0
80-81	32.879886486550035	35.0	33.0	35.5	26.0	37.0
82-83	32.659982834838225	35.0	33.0	35.0	25.0	37.0
84-85	32.47807033013544	35.0	33.0	35.0	25.0	36.5
86-87	32.288014660929264	35.0	33.0	35.0	25.0	36.0
88-89	32.17081540396915	35.0	33.0	35.0	25.0	36.0
90-91	32.02109051683597	35.0	33.0	35.0	24.5	36.0
92-93	31.89161699351016	35.0	33.0	35.0	24.0	35.0
94-95	31.760144786963885	35.0	33.0	35.0	24.0	35.0
96-97	31.63012309537246	35.0	32.0	35.0	23.0	35.0
98-99	31.588075797121896	35.0	32.0	35.0	23.0	35.0
100-101	30.344399572046655	34.0	29.5	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	81.0
8	299.0
9	199.0
10	185.0
11	453.0
12	689.0
13	787.0
14	817.0
15	938.0
16	913.0
17	994.0
18	1151.0
19	1412.0
20	1661.0
21	2443.0
22	2883.0
23	2645.0
24	2289.0
25	2307.0
26	2301.0
27	2454.0
28	2597.0
29	3115.0
30	3770.0
31	4783.0
32	6197.0
33	8808.0
34	14713.0
35	26821.0
36	57203.0
37	88893.0
38	76271.0
39	19146.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30785172624935	18.544227780132825	13.50236682209976	23.645553671518062
2	16.429176072234764	18.773513920240784	38.25949962377727	26.53781038374718
3	21.244239089541008	24.774266365688487	27.335520127915725	26.645974416854777
4	13.595160835214449	15.773725545522948	37.39212989089541	33.238983728367195
5	14.334379702784048	38.43026946952596	32.64878433032355	14.58656649736644
6	30.910811700526715	35.896644563581646	18.244450714823177	14.948093021068473
7	28.103543547780284	30.716234010534237	20.565862490594434	20.614359951091046
8	27.229119638826184	33.53908013544018	19.28641130549285	19.945388920240784
9	26.949891835966895	15.709356188863808	19.69114465763732	37.64960731753198
10-11	27.28393646538751	24.56602120955606	25.822840246425883	22.32720207863055
12-13	27.602256160647105	21.91791290443943	26.731065415726107	23.74876551918736
14-15	24.420989489376904	23.96330911876313	23.99359570930166	27.622105682558306
16-17	24.049689002498866	26.78490798033266	25.27264678067212	23.892756236496353
18-19	24.17766252704864	25.858353325806753	26.300539796782385	23.663444350362216
20-21	25.255164301992206	24.01867235168713	26.47298390061016	24.253179445710504
22-23	25.211115980692927	25.108168445698116	25.609522941122837	24.071192632486124
24-25	24.241159229414652	24.721672639398864	26.21113191849646	24.826036212690024
26-27	24.131307609104592	25.909106941309258	26.534136333709558	23.4254491158766
28-29	23.95289573927765	26.042548438675695	26.479025583145223	23.52553023890143
30-31	24.040632054176072	24.784994591798345	26.650236314898418	24.524137039127165
32-33	23.892788280662153	25.132412763355905	26.86333121708051	24.11146773890143
34-35	24.527811089164786	24.81615053611738	26.37497648607976	24.28106188863807
36-37	23.95288977872249	24.73859912947226	27.21376733301288	24.094743758792376
38-39	23.911283033343082	25.374222345023817	26.111276860603372	24.60321776102973
40-41	24.537985567101224	24.94554593688914	26.561824835755964	23.954643660253677
42-43	24.547846151585073	25.222880322158698	26.46595435105304	23.763319175203186
44-45	24.417533023977754	24.889650600656438	26.119625657209127	24.573190718156685
46-47	24.518405521068473	24.740024219337847	25.790508606094807	24.951061653498872
48-49	24.056063064334086	25.130649219337847	26.1208791854778	24.692408530850262
50-51	24.06605648043642	25.560513073739656	25.593285600075244	24.780144845748683
52-53	24.740465105342363	25.665737866817157	24.72679763920241	24.86699938863807
54-55	24.481958944695258	24.811447752069224	25.496878527088036	25.20971477614748
56-57	24.420234904063207	25.477038656884876	25.21765072422874	24.885075714823177
58-59	24.921653594233234	25.600900763179247	25.561801055986077	23.91564458660144
60-61	24.75577023145603	25.608920744022505	25.10443891095425	24.53087011356721
62-63	24.506189174090736	25.27564611449237	25.757255005733438	24.460909705683456
64-65	25.575445313947437	24.596445722721672	24.89258648221832	24.93552248111257
66-67	24.089924900889457	25.196052923632333	24.87604751426207	25.83797466121614
68-69	24.391430351768246	25.31214729119639	25.980971360045146	24.31545099699022
70-71	24.833932938299476	24.83569648231753	25.607834838224232	24.722535741158765
72-73	24.44433667231001	25.258506160647105	25.82401594243792	24.473141224604966
74-75	24.660076890519186	24.86332533860045	25.86722277088036	24.609375
76-77	24.767506113619262	24.490629702784048	25.142994027464262	25.59887015613243
78-79	24.44110613140639	24.0918571783448	25.859856508069036	25.60718018217978
80-81	24.49530599280017	25.15455518693678	25.222053598738853	25.1280852215242
82-83	24.214179109964196	25.39476683061676	25.570480160568167	24.820573898850878
84-85	24.289895724452638	24.777093192319512	26.09652507949669	24.83648600373116
86-87	24.109498389729563	26.046381670857784	25.269334274495964	24.574785664916693
88-89	24.449462583743873	25.077661096238014	25.01973687112154	25.45313944889657
90-91	23.92159283295711	25.00088177200903	25.261298438675695	25.81622695635816
92-93	24.056200994981005	25.19756325183895	25.983995061837263	24.762240691342782
94-95	24.498859574868323	24.86361926260346	25.791537340105343	24.845983822422873
96-97	24.546328301354404	25.48732599699022	25.642223946576376	24.324121755079005
98-99	23.469476233655726	25.92554490716995	25.62132864614946	24.983650213024866
100-101	24.242023231877667	25.15880017644464	25.178209086898985	25.42096750477871
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	3.0
3	4.5
4	3.0
5	1.5
6	3.5
7	12.0
8	21.5
9	24.5
10	27.5
11	36.0
12	42.5
13	46.5
14	53.5
15	61.5
16	69.0
17	80.5
18	87.0
19	92.5
20	110.5
21	137.5
22	170.5
23	217.5
24	284.5
25	383.5
26	525.5
27	707.5
28	882.0
29	1122.0
30	1493.5
31	1944.5
32	2636.5
33	3368.0
34	4189.0
35	5164.0
36	6254.0
37	7127.5
38	7519.0
39	7750.5
40	7668.0
41	7567.0
42	7633.0
43	7790.0
44	8186.5
45	9270.5
46	10819.5
47	11283.0
48	11848.0
49	13587.5
50	15554.0
51	15984.5
52	15636.0
53	16755.0
54	17909.0
55	17939.5
56	15603.5
57	13421.0
58	12741.0
59	11867.0
60	9915.5
61	7536.0
62	6248.5
63	4563.5
64	2723.5
65	1770.0
66	1439.5
67	1250.0
68	979.0
69	680.5
70	412.5
71	267.5
72	200.0
73	134.5
74	104.0
75	81.0
76	60.0
77	37.5
78	22.0
79	15.5
80	6.5
81	6.5
82	7.0
83	6.5
84	5.5
85	3.0
86	4.0
87	3.0
88	1.5
89	1.5
90	1.5
91	1.5
92	0.5
93	1.0
94	2.0
95	2.5
96	3.0
97	3.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15519187358916478
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.04100239841986456
16-17	0.0792125188111362
18-19	0.02821670428893905
20-21	0.043794676448457484
22-23	0.07201138073739655
24-25	0.01969290820165538
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.025130502257336342
38-39	0.005437594055680963
40-41	0.008523796087283672
42-43	0.005584556057185854
44-45	0.016018858164033107
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.019986832204665163
60-61	0.02145645221971407
62-63	0.033507336343115125
64-65	0.05378809255079007
66-67	0.02160341422121896
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.019252022197140707
80-81	0.06348758465011287
82-83	0.053347206546275394
84-85	0.03394822234762979
86-87	0.0643693566591422
88-89	0.03659353837471783
90-91	0.0
92-93	0.004849746049661399
94-95	0.0
96-97	0.0
98-99	0.0016165820165537999
100-101	0.05114277652370203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	340224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.39391177659926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25650339746493	46.636285872690394
2	11.926350935714868	13.689998668423625
3	3.155332537988229	5.432906319333884
4	1.1989108380444624	2.752407314669302
5	0.6105071812241183	1.751969764907867
6	0.3573075550893305	1.2304366976345646
7	0.2354057022201191	0.9457597873450938
8	0.14880837705647038	0.6832555891518381
9	0.10407019052849711	0.5375695800389795
>10	0.7865438497657149	9.151065899556645
>50	0.12230876855805596	5.016464716330404
>100	0.09380460639410208	10.250958999045032
>500	0.0036278024572304673	1.5351304121542355
>1k	5.182574938900668E-4	0.3857903787180863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1297	0.381219431903687	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	969	0.28481235891647855	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	881	0.2589470466516178	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	878	0.25806527464258844	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	758	0.22279439428141457	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	601	0.17664832580887885	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	558	0.16400959367945825	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	516	0.1516647855530474	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	493	0.1449045334838224	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	489	0.1437288374717833	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	487	0.14314098946576373	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	482	0.14167136945071482	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.13990782543265612	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	476	0.13990782543265612	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	461	0.1354989653875094	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	455	0.1337354213694507	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	452	0.13285364936042138	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	404	0.11874529721595184	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	403	0.11845137321294205	No Hit
GCTTAACCCGCGGTCTAATGTTATTAAATATGTTAAATAAACTTGATGGC	394	0.11580605718585402	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	384	0.1128668171557562	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	377	0.11080934913468773	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.10698833709556056	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	363	0.10669441309255079	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.939240030097818E-4	0.0
2	0.0	0.0	0.0	2.939240030097818E-4	0.0
3	0.0	0.0	0.0	2.939240030097818E-4	0.0
4	0.0	0.0	0.0	2.939240030097818E-4	0.0
5	0.0	0.0	0.0	2.939240030097818E-4	0.0
6	0.0	0.0	0.0	2.939240030097818E-4	0.0
7	0.0	0.0	0.0	2.939240030097818E-4	0.0
8	0.0	0.0	0.0	2.939240030097818E-4	0.0
9	0.0	0.0	0.0	2.939240030097818E-4	0.0
10-11	0.0	0.0	0.0	2.939240030097818E-4	0.0
12-13	0.0	0.0	0.0	5.878480060195636E-4	0.0
14-15	0.0	0.0	0.0	5.878480060195636E-4	0.0
16-17	0.0	0.0	0.0	8.817720090293454E-4	0.0
18-19	0.0	0.0	0.0	0.0011756960120391272	0.0
20-21	0.0	0.0	0.0	0.0016165820165537999	0.0
22-23	0.0	0.0	0.0	0.0020574680210684727	0.0
24-25	0.0	0.0	0.0	0.0023513920240782545	0.0
26-27	0.0	0.0	0.0	0.003086202031602709	0.0
28-29	0.0	0.0	0.0	0.003967974040632054	0.0
30-31	0.0	0.0	0.0	0.005878480060195636	0.0
32-33	0.0	0.0	0.0	0.012344808126410835	0.0
34-35	0.0	0.0	0.0	0.019398984198645598	0.0
36-37	0.0	0.0	0.0	0.028804552294958617	0.0
38-39	0.0	0.0	0.0	0.04702784048156509	0.0
40-41	0.0	0.0	0.0	0.0709826467268623	0.0
42-43	0.0	0.0	0.0	0.08744239089541009	0.0
44-45	0.0	0.0	0.0	0.11536517118133935	0.0
46-47	0.0	0.0	0.0	0.1497542795334838	0.0
48-49	0.0	0.0	0.0	0.1820859198645598	0.0
50-51	0.0	0.0	0.0	0.2183855342362679	0.0
52-53	0.0	0.0	0.0	0.2542442626034612	0.0
54-55	0.0	0.0	0.0	0.2955405850263356	0.0
56-57	0.0	0.0	0.0	0.34785905756207675	0.0
58-59	0.0	0.0	0.0	0.3975322140707299	0.0
60-61	0.0	0.0	0.0	0.45631701467268626	0.0
62-63	0.0	0.0	0.0	0.5236256113619262	2.939240030097818E-4
64-65	0.0	0.0	0.0	0.5881419300225734	2.939240030097818E-4
66-67	0.0	0.0	0.0	0.6600063487584651	2.939240030097818E-4
68-69	0.0	0.0	0.0	0.7450973476297968	2.939240030097818E-4
70-71	0.0	0.0	0.0	0.8382712565838977	2.939240030097818E-4
72-73	0.0	0.0	0.0	0.9349722535741158	2.939240030097818E-4
74-75	0.0	0.0	0.0	1.0422545146726863	2.939240030097818E-4
76-77	0.0	0.0	0.0	1.1507124717832957	2.939240030097818E-4
78-79	0.0	0.0	0.0	1.2737196670428894	2.939240030097818E-4
80-81	0.0	0.0	0.0	1.4059854683972912	2.939240030097818E-4
82-83	0.0	0.0	0.0	1.5567684819413092	2.939240030097818E-4
84-85	0.0	0.0	0.0	1.7197493416102332	2.939240030097818E-4
86-87	0.0	0.0	0.0	1.8975733634311513	2.939240030097818E-4
88-89	0.0	0.0	0.0	2.099499153498871	2.939240030097818E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	125	0.0	64.573715	6
CCGTGCG	140	0.0	61.091476	9
TACGAGT	25	0.005026336	56.976807	4
GGTATCA	465	0.0	55.27696	1
ACCGTGC	155	0.0	55.138847	8
AACCGTG	145	0.0	52.392467	7
GTATCAA	860	0.0	47.046116	1
GACCGTG	70	2.0552124E-4	33.914764	7
ATCAACG	1220	0.0	33.85917	3
CTAATGT	410	0.0	33.58389	3
ATACAAA	285	0.0	33.40323	1
ATGTTAT	390	0.0	32.871235	6
TGGTATC	260	0.0	32.871235	2
GTCTAAT	365	0.0	32.602467	1
AATGCGG	105	1.9025047E-6	31.653784	7
TCAACGC	1300	0.0	31.41029	4
GTTATTA	400	0.0	30.862436	8
TCTAATG	420	0.0	30.52329	2
AAATGCG	110	2.7346578E-6	30.214973	6
TATATGA	80	4.4977714E-4	29.67542	2
>>END_MODULE