FastQCFastQC Report
Fri 10 Feb 2017
SRR3224502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340224
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG8570.251892870579383No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT8120.23866629044394283No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC7470.21956123024830698No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA7130.20956781414597442No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT7080.20809819413092548No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA6400.18811136192626035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.17312123777276148No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA5870.1725333897667419No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG5710.1678306057185854No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT5670.16665490970654626No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG5670.16665490970654626No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG5640.16577313769751695No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG5500.16165820165537997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.1501951655379985No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG4870.14314098946576373No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT4590.13491111738148986No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA4560.1340293453724605No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG4250.12491770127915727No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC4180.12286023325808879No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC4100.12050884123401054No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA4060.11933314522197142No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG3630.10669441309255079No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT3600.10581264108352145No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG3580.10522479307750188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1800.055.4060368
CCGTGCG1650.054.6864789
AAACCGT1500.053.823016
AACCGTG1650.051.8082437
GGTATCA5450.049.0502931
GTATCAA9250.043.3498541
ATGCGGG756.9170346E-637.9927148
GTATAAC1301.9463187E-1036.720341
AATGCGG801.0767957E-535.6181687
TGGTATC2650.034.0500682
AAATGCG902.4111678E-531.6605936
ATCAACG12500.031.5339493
TCAACGC12950.030.8049014
GTCTTAG953.3675486E-530.1493341
ATAACTC953.4882643E-529.9942463
TATCAAC13700.029.4651492
CTAGACG650.00584384229.2251634
GTTCCGG2950.028.977496
GTCTAAT3850.028.5178781
CAACGCA14000.028.4945345
Produced by FastQC (version 0.11.5)