##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340224
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.117131654439426	34.0	33.0	34.0	31.0	34.0
2	33.24340434537246	34.0	33.0	34.0	31.0	34.0
3	33.3592280379985	34.0	34.0	34.0	31.0	34.0
4	36.588571059066965	37.0	37.0	37.0	35.0	37.0
5	36.61816332768999	37.0	37.0	37.0	35.0	37.0
6	36.660347300601956	37.0	37.0	37.0	35.0	37.0
7	36.64309102238525	37.0	37.0	37.0	35.0	37.0
8	36.63000846501129	37.0	37.0	37.0	35.0	37.0
9	38.4483693096313	39.0	39.0	39.0	37.0	39.0
10-11	38.480689193002256	39.0	39.0	39.0	37.0	39.0
12-13	38.46680422310007	39.0	39.0	39.0	37.0	39.0
14-15	40.024035635346124	41.0	40.0	41.0	38.0	41.0
16-17	40.02412675178706	41.0	40.0	41.0	38.0	41.0
18-19	40.02703659941686	41.0	40.0	41.0	38.0	41.0
20-21	40.02664861973288	41.0	40.0	41.0	38.0	41.0
22-23	39.94869409565463	41.0	40.0	41.0	38.0	41.0
24-25	39.89929134922875	41.0	40.0	41.0	38.0	41.0
26-27	39.818475474981184	41.0	40.0	41.0	38.0	41.0
28-29	39.77587706922498	41.0	40.0	41.0	37.0	41.0
30-31	39.69131072469902	41.0	40.0	41.0	37.0	41.0
32-33	39.612124659048156	41.0	40.0	41.0	36.0	41.0
34-35	39.515297274736646	41.0	40.0	41.0	36.0	41.0
36-37	39.371277452501886	41.0	39.0	41.0	35.5	41.0
38-39	39.233406520410085	41.0	39.0	41.0	35.0	41.0
40-41	39.05863636898044	41.0	39.0	41.0	35.0	41.0
42-43	38.90832510346125	41.0	38.5	41.0	35.0	41.0
44-45	38.58929264249436	40.0	38.0	41.0	35.0	41.0
46-47	38.280012579947325	40.0	37.5	41.0	35.0	41.0
48-49	38.100536705229494	40.0	37.0	41.0	34.5	41.0
50-51	37.87023549191122	40.0	36.5	41.0	34.0	41.0
52-53	37.56553329571106	39.5	36.0	41.0	34.0	41.0
54-55	37.331511004514674	39.0	35.0	41.0	33.0	41.0
56-57	37.06113619262604	39.0	35.0	41.0	33.0	41.0
58-59	36.74558820071482	38.0	35.0	41.0	33.0	41.0
60-61	36.458386239653876	37.0	35.0	40.5	33.0	41.0
62-63	36.219523020127916	37.0	35.0	40.0	32.5	41.0
64-65	35.93270462989089	36.0	35.0	40.0	32.0	41.0
66-67	35.646981106565086	36.0	35.0	39.0	32.0	41.0
68-69	35.374550296275395	35.0	35.0	39.0	31.0	41.0
70-71	35.08291302200902	35.0	35.0	39.0	31.0	41.0
72-73	34.79787581123025	35.0	35.0	37.5	31.0	40.0
74-75	34.50520686371332	35.0	35.0	37.0	31.0	39.5
76-77	32.737774231094804	34.5	32.0	35.5	27.5	38.0
78-79	34.08055428188487	35.0	34.0	36.0	31.0	39.0
80-81	34.06200620767494	35.0	35.0	36.0	31.0	37.5
82-83	33.90586789879609	35.0	35.0	35.5	31.0	37.0
84-85	33.73914244732882	35.0	34.0	35.0	31.0	37.0
86-87	33.57465228790444	35.0	34.0	35.0	31.0	36.0
88-89	33.45476656555681	35.0	34.0	35.0	30.5	36.0
90-91	33.332388367663654	35.0	34.0	35.0	30.0	36.0
92-93	33.246325949962376	35.0	34.0	35.0	30.0	36.0
94-95	33.152168865218215	35.0	34.0	35.0	30.0	35.0
96-97	33.111719043924005	35.0	34.0	35.0	30.0	35.0
98-99	33.08929558173439	35.0	34.0	35.0	30.0	35.0
100-101	32.21866182279909	34.5	32.5	35.0	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	24.0
16	67.0
17	141.0
18	295.0
19	489.0
20	720.0
21	1226.0
22	1849.0
23	1831.0
24	1333.0
25	1277.0
26	1522.0
27	1947.0
28	2439.0
29	2752.0
30	3079.0
31	3355.0
32	4103.0
33	5504.0
34	9532.0
35	19678.0
36	54382.0
37	103973.0
38	93475.0
39	25222.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.102498294402395	18.282529659677902	13.265562286061943	23.349409759857764
2	16.332181151241535	18.96838553423627	38.86821623401053	25.831217080511664
3	21.009099887133186	25.511133841234013	27.361091516177577	26.118674755455228
4	13.704206640331076	16.170522949586154	37.56201796463506	32.5632524454477
5	14.301166290443943	37.627563017306244	33.440321670428894	14.63094902182092
6	30.79382994732882	35.42460261474793	18.591868886380738	15.189698551542513
7	28.292242757712565	30.829982599699022	20.666972347629798	20.210802294958615
8	27.28143811136193	33.01795287810384	19.144446012039126	20.556162998495108
9	26.985456640331073	15.799590857787809	19.67968162151994	37.53527088036117
10-11	27.469843397291193	24.312952642964635	25.955987819789318	22.261216139954854
12-13	27.92939357599699	22.161869826937547	26.582780756207676	23.325955840857787
14-15	24.787345983822423	24.243880502257337	24.234915820165536	26.733857693754704
16-17	23.813869685854026	26.96840904815651	25.451026382618508	23.766694883370956
18-19	23.73113007900677	25.803000376222723	27.083480295334837	23.38238924943567
20-21	24.973105479427897	24.221234620155315	27.129516956774776	23.676142943642017
22-23	25.096334441436596	24.945256171723674	26.206495189891278	23.751914196948455
24-25	24.58912554982886	24.549886176424337	26.521113575206595	24.339874698540207
26-27	24.239266697921458	25.8913126865551	26.512391447160542	23.3570291683629
28-29	23.91422243320581	25.7707473865061	27.062172716196535	23.252857464091555
30-31	23.875433543001588	24.656986655693373	27.169331608958906	24.29824819234613
32-33	23.820224058596775	25.104232952750177	27.53822119871202	23.537321789941025
34-35	24.672515075402735	24.84579218797995	26.531973962872552	23.94971877374477
36-37	23.698404253755694	24.79079528653771	27.19705364517352	24.313746814533076
38-39	23.93658295711061	25.066867710684726	26.468297357035365	24.528251975169297
40-41	24.209344431903688	25.07010087471783	26.895956781414597	23.82459791196388
42-43	24.236679364183598	25.234992240406324	26.949891835966895	23.57843655944319
44-45	24.45181288664112	24.691532219542342	26.724075845962325	24.13257904785421
46-47	24.30998548266819	24.735797798719474	26.12207796410233	24.83213875451001
48-49	23.91111698961709	24.621828362321715	26.688711064083066	24.77834358397813
50-51	24.64876081640331	25.032625564334086	25.997431104213693	24.321182515048907
52-53	24.649348664409327	25.645310148607976	25.271144892776526	24.43419629420617
54-55	24.648613854401805	24.794988007900677	25.913368839352895	24.643029298344622
56-57	24.768920315170792	25.21777854857413	25.423688956235583	24.589612180019486
58-59	25.168747803815883	25.29504217408628	25.59320862683175	23.94300139526609
60-61	24.77007303562398	25.51836475472497	25.479847577828217	24.231714631822832
62-63	24.64097183032355	25.00484974604966	25.916308079382993	24.43787034424379
64-65	24.959585449586154	24.727679411211437	25.577119779909708	24.7356153592927
66-67	24.28150277464259	25.0119039221219	25.568595983822423	25.137997319413092
68-69	24.79924990594432	24.83804787434161	25.98420452407825	24.37849769563582
70-71	25.143434913468777	24.78808079382995	25.78507101203913	24.283413280662153
72-73	24.415385158013546	24.911675837095558	26.01771186042137	24.655227144469528
74-75	24.73047168924003	24.703871566967646	25.876481376975168	24.689175366817157
76-77	24.7610019913439	24.201515184915753	25.681135139504295	25.356347684236052
78-79	24.493005399907698	24.047961292332836	25.815056835718757	25.64397647204071
80-81	24.107390875567503	25.08065191734777	25.907820921144104	24.904136285940627
82-83	24.16190136073917	25.06440694904319	26.128131985380026	24.645559704837613
84-85	24.494549794028213	24.71455676018065	26.2088993332187	24.581994112572435
86-87	24.057238760346124	25.65985938675696	25.761263167795335	24.52163868510158
88-89	24.633917654251317	24.52046298908954	25.196047310007526	25.64957204665162
90-91	24.39098946576373	24.902858117005266	25.2463083145222	25.459844102708807
92-93	24.511645268999246	25.12682820729872	25.939234151617757	24.42229237208427
94-95	24.541478555304742	24.8046875	25.611802812264862	25.0420311324304
96-97	24.62054411211437	25.34374412151994	25.70703418924003	24.32867757712566
98-99	22.93386122084274	25.903816309255077	26.13513449962378	25.0271879702784
100-101	23.878516217837856	25.359415993129332	25.46368192566971	25.298385863363098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	3.0
3	5.5
4	4.0
5	2.0
6	8.0
7	15.0
8	25.0
9	38.5
10	44.0
11	44.5
12	48.0
13	59.5
14	61.0
15	65.0
16	76.0
17	85.5
18	100.0
19	115.5
20	124.0
21	155.0
22	190.5
23	245.0
24	327.0
25	425.0
26	582.5
27	768.0
28	915.0
29	1105.5
30	1442.5
31	1898.0
32	2565.5
33	3389.0
34	4271.0
35	5199.5
36	6298.0
37	7127.0
38	7519.0
39	7799.0
40	7737.0
41	7571.0
42	7681.5
43	8116.5
44	8809.0
45	9666.5
46	10543.0
47	11448.5
48	12343.0
49	13959.5
50	16085.5
51	16159.0
52	15136.0
53	16154.5
54	17845.0
55	17866.5
56	15390.5
57	13152.5
58	12710.5
59	12227.5
60	10114.5
61	7426.0
62	6019.5
63	4250.5
64	2510.0
65	1616.0
66	1212.0
67	1037.5
68	786.5
69	515.5
70	301.5
71	211.0
72	149.5
73	98.5
74	73.0
75	57.0
76	38.5
77	21.0
78	12.5
79	6.5
80	4.5
81	3.5
82	2.5
83	1.5
84	1.0
85	1.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4799778969149736
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0017635440180586905
22-23	8.817720090293453E-4
24-25	0.0013226580135440181
26-27	0.002792278028592927
28-29	0.005437594055680963
30-31	0.001175696012039127
32-33	1.4696200150489088E-4
34-35	0.005143670052671181
36-37	0.0020574680210684723
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.010140378103837472
46-47	0.0839153028592927
48-49	4.4088600451467263E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008082910082769
58-59	0.04276594243792325
60-61	0.03409518434913469
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	4.4088600451467263E-4
78-79	0.009699492099322799
80-81	0.008082910082769
82-83	0.0013226580135440181
84-85	0.0022044300225733636
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06701467268623025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	340224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.26421282703179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.88962729006909	44.15037365230625
2	12.444471883391575	13.754678853675303
3	3.59482294060215	5.959951801948005
4	1.3681695095265303	3.024432438317195
5	0.6804660294328584	1.8802709736071381
6	0.3753145593743087	1.2444878211807275
7	0.24933095792685953	0.9645355386266372
8	0.17976918386737373	0.7947841949590683
9	0.1203784751382263	0.5987359502849245
>10	0.8545095488701413	9.522383344542398
>50	0.12690182015046217	4.912137130792884
>100	0.10877298870039606	10.546214031858263
>500	0.007464812950027181	2.647014267901167
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	857	0.251892870579383	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	812	0.23866629044394283	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	747	0.21956123024830698	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	713	0.20956781414597442	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	708	0.20809819413092548	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	640	0.18811136192626035	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	589	0.17312123777276148	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	587	0.1725333897667419	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	571	0.1678306057185854	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	567	0.16665490970654626	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	567	0.16665490970654626	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	564	0.16577313769751695	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	550	0.16165820165537997	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.1501951655379985	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	487	0.14314098946576373	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	459	0.13491111738148986	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	456	0.1340293453724605	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	425	0.12491770127915727	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	418	0.12286023325808879	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	410	0.12050884123401054	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	406	0.11933314522197142	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	363	0.10669441309255079	No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT	360	0.10581264108352145	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	358	0.10522479307750188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.939240030097818E-4	0.0
2	0.0	0.0	0.0	2.939240030097818E-4	0.0
3	0.0	0.0	0.0	2.939240030097818E-4	0.0
4	0.0	0.0	0.0	2.939240030097818E-4	0.0
5	0.0	0.0	0.0	2.939240030097818E-4	0.0
6	0.0	0.0	0.0	2.939240030097818E-4	0.0
7	0.0	0.0	0.0	2.939240030097818E-4	0.0
8	0.0	0.0	0.0	2.939240030097818E-4	0.0
9	0.0	0.0	0.0	2.939240030097818E-4	0.0
10-11	0.0	0.0	0.0	2.939240030097818E-4	0.0
12-13	0.0	0.0	0.0	2.939240030097818E-4	0.0
14-15	0.0	0.0	0.0	2.939240030097818E-4	0.0
16-17	0.0	0.0	0.0	5.878480060195636E-4	0.0
18-19	0.0	0.0	0.0	8.817720090293454E-4	0.0
20-21	0.0	0.0	0.0	0.0013226580135440181	0.0
22-23	0.0	0.0	0.0	0.0017635440180586907	0.0
24-25	0.0	0.0	0.0	0.0020574680210684727	0.0
26-27	0.0	0.0	0.0	0.002792278028592927	0.0
28-29	0.0	0.0	0.0	0.0038210120391271637	0.0
30-31	0.0	0.0	0.0	0.005878480060195636	0.0
32-33	0.0	0.0	0.0	0.012638732129420616	0.0
34-35	0.0	0.0	0.0	0.019839870203160272	0.0
36-37	0.0	0.0	0.0	0.029098476297968398	0.0
38-39	0.0	0.0	0.0	0.04790961249059443	0.0
40-41	0.0	0.0	0.0	0.07289315274642588	0.0
42-43	0.0	0.0	0.0	0.08905897291196388	0.0
44-45	0.0	0.0	0.0	0.11727567720090294	0.0
46-47	0.0	0.0	0.0	0.15225263355906696	0.0
48-49	0.0	0.0	0.0	0.18487819789315274	0.0
50-51	0.0	0.0	0.0	0.22176566027088035	0.0
52-53	0.0	0.0	0.0	0.2582122366440933	0.0
54-55	0.0	0.0	0.0	0.3005372930775019	0.0
56-57	0.0	0.0	0.0	0.355648043641836	0.0
58-59	0.0	0.0	0.0	0.405468162151994	0.0
60-61	0.0	0.0	0.0	0.464693848758465	0.0
62-63	0.0	0.0	0.0	0.5325902934537246	0.0
64-65	0.0	0.0	0.0	0.5981353461249059	0.0
66-67	0.0	0.0	0.0	0.6714693848758465	0.0
68-69	0.0	0.0	0.0	0.7580300037622272	0.0
70-71	0.0	0.0	0.0	0.8529674567343868	0.0
72-73	0.0	0.0	0.0	0.9515789597441685	0.0
74-75	0.0	0.0	0.0	1.0606247648607976	0.0
76-77	0.0	0.0	0.0	1.1711401899924756	0.0
78-79	0.0	0.0	0.0	1.2970866252821671	0.0
80-81	0.0	0.0	0.0	1.431703818660647	0.0
82-83	0.0	0.0	0.0	1.583956452219714	0.0
84-85	0.0	0.0	0.0	1.75090528592927	0.0
86-87	0.0	0.0	0.0	1.930198927765237	0.0
88-89	0.0	0.0	0.0	2.136386615876599	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTGC	180	0.0	55.406036	8
CCGTGCG	165	0.0	54.686478	9
AAACCGT	150	0.0	53.82301	6
AACCGTG	165	0.0	51.808243	7
GGTATCA	545	0.0	49.050293	1
GTATCAA	925	0.0	43.349854	1
ATGCGGG	75	6.9170346E-6	37.992714	8
GTATAAC	130	1.9463187E-10	36.72034	1
AATGCGG	80	1.0767957E-5	35.618168	7
TGGTATC	265	0.0	34.050068	2
AAATGCG	90	2.4111678E-5	31.660593	6
ATCAACG	1250	0.0	31.533949	3
TCAACGC	1295	0.0	30.804901	4
GTCTTAG	95	3.3675486E-5	30.149334	1
ATAACTC	95	3.4882643E-5	29.994246	3
TATCAAC	1370	0.0	29.465149	2
CTAGACG	65	0.005843842	29.225163	4
GTTCCGG	295	0.0	28.97749	6
GTCTAAT	385	0.0	28.517878	1
CAACGCA	1400	0.0	28.494534	5
>>END_MODULE