FastQCFastQC Report
Fri 10 Feb 2017
SRR3224501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977707
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG33550.3431498393690543No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA25590.26173485512530853No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA20780.21253811213379878No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA17590.17991075035772475No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16190.1655915320233976No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC15310.1565908804989634No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA15170.15515895866553067No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA15110.1545452778797738No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT15040.15382931696305743No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14950.15290879578442212No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC14420.1474879488435697No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12710.1299980464494987No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA12700.1298957663185392No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA11940.12212247636561875No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11820.12089511479410497No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC11010.11261042418638713No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG10510.10749641763841314No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG10500.10739413750745366No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA10470.10708729711457522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7850.056.330381
GTATCAA17500.049.4495931
ATCAACG22000.038.6251953
TCAACGC23100.036.78594
GTCTAAT3000.033.2833821
CGCAGAG27900.030.8005988
TATCAAC28500.030.6487882
ACGCAGA28750.029.7217927
AATGCGG2900.029.465577
AAATGCG3050.028.0164436
TCGCACC11050.027.9692271
CAACGCA31300.027.300375
CGAATAC350.008406427.13397240-41
AACGCAG31700.027.1056396
GTACATG49950.026.6533581
GTATTGG2750.025.9351021
ATGCGGG3300.025.8966358
TACATGG51950.025.5866152
CGCACCA12100.025.5016542
TCGGTTG1501.0553085E-625.3457859
Produced by FastQC (version 0.11.5)