##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224501_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977707
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.200104939414366	34.0	31.0	34.0	31.0	34.0
2	32.36279273851982	34.0	31.0	34.0	31.0	34.0
3	32.509164811134625	34.0	31.0	34.0	31.0	34.0
4	35.79503368596113	37.0	37.0	37.0	35.0	37.0
5	35.807866773992615	37.0	37.0	37.0	35.0	37.0
6	35.90845110038079	37.0	37.0	37.0	35.0	37.0
7	35.87417805129758	37.0	37.0	37.0	35.0	37.0
8	35.912554579234886	37.0	37.0	37.0	35.0	37.0
9	37.641600193104885	39.0	39.0	39.0	35.0	39.0
10-11	37.715583503033116	39.0	39.0	39.0	35.0	39.0
12-13	37.705492033911995	39.0	39.0	39.0	35.0	39.0
14-15	39.1895977015609	41.0	40.0	41.0	36.0	41.0
16-17	39.0323905832729	41.0	40.0	41.0	36.0	41.0
18-19	39.11332280529852	41.0	40.0	41.0	36.0	41.0
20-21	39.17052552554088	41.0	40.0	41.0	36.0	41.0
22-23	39.11923050566274	41.0	40.0	41.0	36.0	41.0
24-25	39.059348557389896	41.0	39.5	41.0	35.5	41.0
26-27	38.88155091453779	41.0	39.0	41.0	35.0	41.0
28-29	38.808578643704095	41.0	39.0	41.0	35.0	41.0
30-31	38.74467708628454	41.0	39.0	41.0	35.0	41.0
32-33	38.55809000037844	41.0	39.0	41.0	35.0	41.0
34-35	38.55176397427859	40.0	39.0	41.0	35.0	41.0
36-37	38.543566221782186	40.0	39.0	41.0	35.0	41.0
38-39	38.41764199294881	40.0	38.0	41.0	34.5	41.0
40-41	38.3098852723771	40.0	38.0	41.0	34.0	41.0
42-43	38.156519284407295	40.0	38.0	41.0	33.5	41.0
44-45	37.96798427340707	40.0	38.0	41.0	33.0	41.0
46-47	37.78488493996667	40.0	37.0	41.0	33.0	41.0
48-49	37.56444057370971	40.0	37.0	41.0	33.0	41.0
50-51	36.25010100162932	38.5	35.0	40.0	30.5	40.5
52-53	36.561637586720764	39.0	35.0	40.0	31.5	40.5
54-55	36.985665951046684	39.0	35.0	41.0	32.0	41.0
56-57	36.814274624197225	39.0	35.0	41.0	32.0	41.0
58-59	36.65915606618343	39.0	35.0	41.0	32.0	41.0
60-61	36.397572585651936	38.0	35.0	41.0	31.0	41.0
62-63	36.11685862942579	37.0	35.0	41.0	31.0	41.0
64-65	35.86472225319037	37.0	35.0	40.0	31.0	41.0
66-67	35.646541857632194	36.0	35.0	40.0	30.5	41.0
68-69	35.360541552837404	36.0	35.0	39.5	30.0	41.0
70-71	35.08440156406776	35.0	35.0	39.0	30.0	41.0
72-73	34.780186702151056	35.0	34.5	39.0	29.0	41.0
74-75	34.51292923135459	35.0	34.0	37.5	29.0	40.0
76-77	34.200299271663184	35.0	34.0	37.0	29.0	39.0
78-79	33.976027582905715	35.0	34.0	37.0	29.0	39.0
80-81	33.68279453865013	35.0	34.0	36.0	29.0	39.0
82-83	33.43076504515156	35.0	34.0	36.0	29.0	37.0
84-85	33.20990337596028	35.0	34.0	35.5	28.0	37.0
86-87	32.997490556986904	35.0	34.0	35.0	27.5	36.5
88-89	32.85162374821905	35.0	33.5	35.0	27.0	36.0
90-91	32.67958907934586	35.0	33.0	35.0	27.0	36.0
92-93	32.546464329292924	35.0	33.0	35.0	27.0	36.0
94-95	32.417177641154254	35.0	33.0	35.0	26.0	35.5
96-97	32.29059523967814	35.0	33.0	35.0	25.5	35.0
98-99	32.247566499984146	35.0	33.0	35.0	25.0	35.0
100-101	31.010684182480027	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	237.0
8	661.0
9	497.0
10	569.0
11	1001.0
12	1543.0
13	1737.0
14	1998.0
15	1908.0
16	2115.0
17	2221.0
18	2496.0
19	2874.0
20	3308.0
21	4334.0
22	5181.0
23	5077.0
24	5154.0
25	5465.0
26	5557.0
27	5927.0
28	6549.0
29	7613.0
30	9363.0
31	11827.0
32	16090.0
33	22923.0
34	38302.0
35	70001.0
36	150617.0
37	244163.0
38	263379.0
39	77007.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.437193012647754	19.169225001306057	13.326490620464012	24.06709136558218
2	16.151362320204317	19.78701185528998	38.50243477851749	25.559191045988218
3	20.37522488843795	26.139835349445185	27.81119496945404	25.673744792662834
4	13.039796176155024	16.412483494543867	38.5824178409278	31.965302488373304
5	13.991205954340105	39.11652468479821	33.473320739239874	13.418948621621816
6	29.905176090587464	36.78576506049359	19.282054848743027	14.027004000175921
7	27.627295293988897	30.946285543624008	21.920166266580885	19.50625289580621
8	26.274436001787855	34.22896634676851	20.601161697727438	18.895435953716195
9	27.214901805960274	15.412081533629197	19.844288728627287	37.528727931783244
10-11	26.43394186601917	24.83443403800934	27.19357639865522	21.53804769731627
12-13	26.602550661905866	22.495082882704125	27.558511905918643	23.343854549471367
14-15	23.747952431963444	24.544675095331495	24.65691405278812	27.05045841991694
16-17	23.680096371681913	27.281036138868973	25.818317476936965	23.22055001251215
18-19	23.764730194097385	26.761613055358623	26.23097312631522	23.24268362422877
20-21	24.90242753239498	24.99262035472433	26.031909739648164	24.073042373232532
22-23	24.959075806599348	25.797414112923654	25.294042696253193	23.94946738422381
24-25	24.135327770119762	25.665731508026425	25.882497359476858	24.31644336237696
26-27	23.899695921170657	26.807264344021263	26.08532003964378	23.207719695164297
28-29	23.665525561338928	26.818208318033932	26.19465749963946	23.32160862098768
30-31	23.81066106717043	25.814482252863076	26.2061640143724	24.168692665594087
32-33	23.65355878601667	26.05187443682003	26.504412876250242	23.790153900913054
34-35	24.414522960355196	25.410271175311212	25.982119387505666	24.193086476827926
36-37	23.378500654978165	25.784196330792362	26.708991742825035	24.128311271404435
38-39	23.706696833597395	26.105356505409283	25.79865823983733	24.389288421155992
40-41	24.302989543884234	25.605234962141648	26.41149891449715	23.680276579476963
42-43	24.092954750020457	26.000480729891173	26.330547827510024	23.57601669257835
44-45	24.132994687086367	25.423938593602003	26.126206759695503	24.316859959616128
46-47	24.031483869911945	25.433181924646135	25.966214827141464	24.569119378300453
48-49	23.492416439690007	25.90796629256004	26.283948053967087	24.31566921378286
50-51	23.77159005714391	26.09882101693043	25.967237628451063	24.1623512974746
52-53	24.048513511716703	25.97869300311852	25.320162379935912	24.652631105228867
54-55	24.15861807269458	25.290092021433825	25.87313990796834	24.678149997903258
56-57	24.049996573615612	25.723708636636538	25.842711569007893	24.383583220739958
58-59	24.207226007078706	25.881002066327053	26.18133554287117	23.73043638372307
60-61	23.98447377789053	26.19986047063541	25.698260588478394	24.117405162995667
62-63	23.61355937752251	25.673411093571126	26.54736973489154	24.16565979401483
64-65	24.509741724829144	25.00140695043748	25.71700752385932	24.77184380087405
66-67	23.719601827803107	25.742787962359614	25.3719693893104	25.165640820526882
68-69	23.858323608197548	25.72877150311903	26.647042518873242	23.76586236981018
70-71	24.247448366432888	25.395235996060165	26.267174112489734	24.09014152501721
72-73	23.931402761768098	25.757154239460288	26.421105709583752	23.890337289187865
74-75	24.043757485627086	25.388076386893005	26.441970856299484	24.126195271180425
76-77	23.790358461174975	25.056791042715247	25.81755065679186	25.335299839317916
78-79	24.064104931341355	24.472317883369044	26.450265893375658	25.013311291913947
80-81	23.772836701434994	25.69617241405776	25.851209983805486	24.67978090070176
82-83	23.432748995789762	25.961655055126037	26.215783816085885	24.389812132998316
84-85	23.688300102409016	24.943705733165686	26.800470201636294	24.567523962789004
86-87	23.730822976559555	26.258969062458902	25.926583525937176	24.083624435044364
88-89	23.951205637768467	25.218690659376637	25.7097854734154	25.120318229439498
90-91	23.591679306786183	25.109925570748704	25.970664012838203	25.32773110962691
92-93	23.582670883434727	25.357272902082883	26.51347470143727	24.546581513045115
94-95	23.882154878711106	24.93124218196249	26.58183893538657	24.60476400393983
96-97	24.275115141857427	25.568703098167443	26.291158803199732	23.865022956775395
98-99	23.00819831001589	25.901821772800798	26.384126443500396	24.705853473682918
100-101	23.832414982170175	25.163231163568827	25.94462265743711	25.059731196823886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	2.5
4	3.0
5	2.0
6	3.0
7	6.0
8	12.5
9	18.0
10	21.5
11	27.0
12	29.0
13	30.0
14	33.0
15	37.0
16	54.5
17	59.5
18	55.5
19	68.0
20	79.5
21	90.5
22	110.0
23	148.0
24	177.5
25	210.0
26	286.5
27	388.5
28	537.5
29	791.5
30	1256.5
31	2239.0
32	4053.5
33	7071.0
34	10842.0
35	15575.0
36	22310.0
37	28998.0
38	33328.5
39	35407.5
40	35651.0
41	34557.0
42	32962.5
43	31992.0
44	31023.0
45	30708.5
46	32278.0
47	32439.0
48	33690.0
49	37513.0
50	41433.0
51	42133.0
52	41468.5
53	42583.0
54	44583.5
55	45163.0
56	38280.5
57	31915.5
58	30159.5
59	28792.5
60	24514.0
61	18720.0
62	15480.0
63	11245.5
64	6766.0
65	4272.0
66	3274.0
67	2961.5
68	2339.0
69	1476.5
70	875.0
71	559.5
72	391.0
73	303.5
74	239.5
75	164.0
76	107.5
77	71.5
78	56.0
79	37.0
80	20.5
81	18.0
82	17.5
83	12.5
84	10.5
85	13.5
86	10.0
87	7.5
88	5.5
89	2.5
90	4.5
91	5.0
92	4.0
93	5.5
94	4.0
95	4.0
96	5.0
97	3.5
98	3.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1519882746057868
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.033547882954709334
16-17	0.06714690597489842
18-19	0.021274267239571775
20-21	0.03646286668705451
22-23	0.06090781798637015
24-25	0.01580228023323961
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.01979120534065932
38-39	0.0036820847145412686
40-41	0.005062866482494244
42-43	0.0027615635359059514
44-45	0.00910293165539369
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.012989576631853919
60-61	0.013807817679529755
62-63	0.026388273787545758
64-65	0.04254853447914355
66-67	0.014319218334327154
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01570000010228013
80-81	0.05364592868824709
82-83	0.045668078473407675
84-85	0.025979153263707838
86-87	0.05405504921208502
88-89	0.030581759156884426
90-91	0.0
92-93	0.002352443012068033
94-95	0.0
96-97	0.0
98-99	5.625407202771383E-4
100-101	0.04213941395530563
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	977707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.310412550184545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01136424884616	31.46542845617799
2	15.238465714087926	13.50445404846157
3	5.817319999997645	7.733035474090055
4	2.7142485211978027	4.810778869520118
5	1.4848728251386774	3.289766373322642
6	0.9054771651127909	2.4073240044551603
7	0.5819217083253313	1.804963367746251
8	0.40288842893563936	1.428172199826711
9	0.29464694855742746	1.1750335062509352
>10	1.2946902500403061	10.148616502268458
>50	0.11457031459413435	3.576799649212274
>100	0.12180047299446685	11.574869987399994
>500	0.013300051628800118	4.026990235143762
>1k	0.004433350542933374	3.053767326124064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3355	0.3431498393690543	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2559	0.26173485512530853	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2078	0.21253811213379878	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1759	0.17991075035772475	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1619	0.1655915320233976	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1531	0.1565908804989634	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1517	0.15515895866553067	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1511	0.1545452778797738	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1504	0.15382931696305743	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1495	0.15290879578442212	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1442	0.1474879488435697	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1271	0.1299980464494987	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1270	0.1298957663185392	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1194	0.12212247636561875	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1182	0.12089511479410497	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1101	0.11261042418638713	No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG	1051	0.10749641763841314	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	1050	0.10739413750745366	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1047	0.10708729711457522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.0456026191895937E-4	0.0
18-19	0.0	0.0	0.0	2.0456026191895937E-4	0.0
20-21	0.0	0.0	0.0	2.557003273986992E-4	0.0
22-23	0.0	0.0	0.0	4.6026058931765857E-4	0.0
24-25	0.0	0.0	0.0	0.001022801309594797	0.0
26-27	0.0	0.0	0.0	0.001994462553709854	0.0
28-29	0.0	0.0	0.0	0.0034263843871425695	0.0
30-31	0.0	0.0	0.0	0.007773289952920455	0.0
32-33	0.0	0.0	0.0	0.018666123900105042	0.0
34-35	0.0	0.0	0.0	0.03359902302018908	0.0
36-37	0.0	0.0	0.0	0.05901563556361977	0.0
38-39	0.0	0.0	0.0	0.10396775312031109	0.0
40-41	0.0	0.0	0.0	0.15408501729045615	0.0
42-43	0.0	0.0	0.0	0.2034351804784051	0.0
44-45	0.0	0.0	0.0	0.262808796500383	0.0
46-47	0.0	0.0	0.0	0.33445602823749854	0.0
48-49	0.0	0.0	0.0	0.4079443023318847	0.0
50-51	0.0	0.0	0.0	0.48383615950381864	0.0
52-53	0.0	0.0	0.0	0.5646374629618076	0.0
54-55	0.0	0.0	0.0	0.6491719911998175	0.0
56-57	0.0	0.0	0.0	0.7466449560042017	0.0
58-59	0.0	0.0	0.0	0.8495387677494383	0.0
60-61	0.0	0.0	0.0	0.964399354816934	0.0
62-63	0.0	0.0	0.0	1.0931700396949189	0.0
64-65	0.0	0.0	0.0	1.214065154489024	0.0
66-67	0.0	0.0	0.0	1.3554674355405045	0.0
68-69	0.0	0.0	0.0	1.5211101076293818	0.0
70-71	0.0	0.0	0.0	1.6900768839744422	0.0
72-73	0.0	0.0	0.0	1.8736185789812285	0.0
74-75	0.0	0.0	0.0	2.0623254206014687	0.0
76-77	0.0	0.0	0.0	2.2658117411453533	0.0
78-79	0.0	0.0	0.0	2.494100993446912	0.0
80-81	0.0	0.0	0.0	2.75200034366124	0.0
82-83	0.0	0.0	0.0	3.0300488796745855	0.0
84-85	0.0	0.0	0.0	3.312802301712067	0.0
86-87	0.0	0.0	0.0	3.612227385095944	0.0
88-89	0.0	0.0	0.0	3.9431036087498605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	785	0.0	56.33038	1
GTATCAA	1750	0.0	49.449593	1
ATCAACG	2200	0.0	38.625195	3
TCAACGC	2310	0.0	36.7859	4
GTCTAAT	300	0.0	33.283382	1
CGCAGAG	2790	0.0	30.800598	8
TATCAAC	2850	0.0	30.648788	2
ACGCAGA	2875	0.0	29.721792	7
AATGCGG	290	0.0	29.46557	7
AAATGCG	305	0.0	28.016443	6
TCGCACC	1105	0.0	27.969227	1
CAACGCA	3130	0.0	27.30037	5
CGAATAC	35	0.0084064	27.133972	40-41
AACGCAG	3170	0.0	27.105639	6
GTACATG	4995	0.0	26.653358	1
GTATTGG	275	0.0	25.935102	1
ATGCGGG	330	0.0	25.896635	8
TACATGG	5195	0.0	25.586615	2
CGCACCA	1210	0.0	25.501654	2
TCGGTTG	150	1.0553085E-6	25.345785	9
>>END_MODULE