FastQCFastQC Report
Fri 10 Feb 2017
SRR3224501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977707
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG22070.22573224902757166No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT19910.20363974074032407No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA19680.20128729772825601No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA19550.19995765602578278No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA18560.18983192306079427No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG17360.17755830734565672No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG15670.16027296521350465No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG15190.15536351892744962No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT14930.15270423552250317No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG14090.14411270452190686No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC13150.1344983722117158No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG13130.13429381194979684No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT13120.13419153181883733No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12360.12641824186591688No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC12340.12621368160399793No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG11750.12017915387738862No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA11160.11414462615077932No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA11070.11322410497214401No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT10750.10995114078144066No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG10400.10637133619785885No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC10030.10258697135235811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.049.435721
GTATCAA18350.047.6203041
CCGTGCG1950.043.8338559
AAACCGT2050.039.3791966
ATCAACG22850.036.7839933
AACCGTG2350.036.3727767
TCAACGC23600.035.615014
TCGGCGC550.002582988234.5357677
TGCGCCG550.002582988234.5357675
ACCGTGC2550.033.5200088
ACGCAGA27550.030.5086827
CGCAGAG27700.030.3434738
TATCACG804.5005415E-429.6791742
TCGCACC9500.029.6555181
CAACGCA28400.029.5955725
AACGCAG28850.029.1339426
CGCACCA9900.028.7798042
GTACATG50200.028.250741
TACATGG51200.027.4532362
TTGCGCC700.00840245627.1352444
Produced by FastQC (version 0.11.5)