##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977707
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.14574100420678	34.0	33.0	34.0	31.0	34.0
2	33.27230039265342	34.0	33.0	34.0	31.0	34.0
3	33.39205815239126	34.0	34.0	34.0	31.0	34.0
4	36.613390310184954	37.0	37.0	37.0	35.0	37.0
5	36.645086922769295	37.0	37.0	37.0	35.0	37.0
6	36.69077954847413	37.0	37.0	37.0	36.0	37.0
7	36.67213592620284	37.0	37.0	37.0	35.0	37.0
8	36.661146948932554	37.0	37.0	37.0	35.0	37.0
9	38.506942263888874	39.0	39.0	39.0	37.0	39.0
10-11	38.53018798065269	39.0	39.0	39.0	37.5	39.0
12-13	38.5171355017403	39.0	39.0	39.0	37.0	39.0
14-15	40.113713515398786	41.0	40.0	41.0	38.0	41.0
16-17	40.12387760341288	41.0	40.0	41.0	38.0	41.0
18-19	40.11271372711866	41.0	40.0	41.0	38.0	41.0
20-21	40.11322870757804	41.0	40.0	41.0	38.0	41.0
22-23	40.04178450190088	41.0	40.0	41.0	38.0	41.0
24-25	40.0060708371731	41.0	40.0	41.0	38.0	41.0
26-27	39.939899172246896	41.0	40.0	41.0	38.0	41.0
28-29	39.9150399864172	41.0	40.0	41.0	38.0	41.0
30-31	39.867281813467635	41.0	40.0	41.0	38.0	41.0
32-33	39.82680547444173	41.0	40.0	41.0	38.0	41.0
34-35	39.75119028502404	41.0	40.0	41.0	37.0	41.0
36-37	39.631791528545875	41.0	40.0	41.0	37.0	41.0
38-39	39.51889625419476	41.0	40.0	41.0	36.5	41.0
40-41	39.389100722404564	41.0	39.0	41.0	36.0	41.0
42-43	39.2899350214328	41.0	39.0	41.0	35.5	41.0
44-45	39.03718240740835	41.0	39.0	41.0	35.0	41.0
46-47	38.808224754450976	40.0	38.0	41.0	35.0	41.0
48-49	38.697329056660124	40.0	38.0	41.0	35.0	41.0
50-51	38.52532456042557	40.0	37.0	41.0	35.0	41.0
52-53	38.25595858472937	40.0	37.0	41.0	35.0	41.0
54-55	38.04918907198169	40.0	36.0	41.0	35.0	41.0
56-57	37.797136054053	39.0	35.5	41.0	34.0	41.0
58-59	37.518754596213384	39.0	35.0	41.0	34.0	41.0
60-61	37.259882050552974	39.0	35.0	41.0	34.0	41.0
62-63	37.04338518595039	38.0	35.0	41.0	34.0	41.0
64-65	36.78004146436509	37.0	35.0	40.5	33.0	41.0
66-67	36.510845273686286	37.0	35.0	40.0	33.0	41.0
68-69	36.258773845334034	36.0	35.0	40.0	33.0	41.0
70-71	35.98552736146924	36.0	35.0	39.0	33.0	41.0
72-73	35.69468153547024	35.0	35.0	39.0	33.0	41.0
74-75	35.3894183022112	35.0	35.0	38.5	32.5	40.5
76-77	33.55911689289327	34.5	32.5	35.5	29.0	39.0
78-79	34.85777743229822	35.0	34.5	37.0	32.0	39.0
80-81	34.78305565982447	35.0	35.0	36.5	33.0	39.0
82-83	34.584062505433636	35.0	35.0	36.0	33.0	37.0
84-85	34.38816843901087	35.0	35.0	36.0	33.0	37.0
86-87	34.208968535563315	35.0	35.0	35.5	32.0	37.0
88-89	34.06848115028326	35.0	35.0	35.0	32.0	36.0
90-91	33.929260504425145	35.0	35.0	35.0	32.0	36.0
92-93	33.841215210691956	35.0	34.5	35.0	32.0	36.0
94-95	33.73914373119963	35.0	34.0	35.0	32.0	36.0
96-97	33.705575392218734	35.0	34.0	35.0	31.5	35.0
98-99	33.68128999792371	35.0	34.0	35.0	31.0	35.0
100-101	32.841141057597014	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	8.0
15	49.0
16	90.0
17	203.0
18	375.0
19	551.0
20	796.0
21	1120.0
22	1672.0
23	1734.0
24	1971.0
25	2563.0
26	3361.0
27	4814.0
28	6001.0
29	6848.0
30	7353.0
31	8117.0
32	9723.0
33	13337.0
34	22824.0
35	48475.0
36	141051.0
37	280651.0
38	313856.0
39	100155.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.952300004727654	18.96406775759046	13.248023120294183	23.8356091173877
2	16.03844505562505	19.87926853341543	38.92024911348697	25.162037297472555
3	20.031256808021215	26.587106362130985	28.01330050822997	25.368336321617825
4	13.109039824814591	16.646193593786275	38.78186409629879	31.46290248510034
5	13.80904504110127	38.22699438584361	34.36919240631396	13.594768166741162
6	29.989557198629036	36.29369535044752	19.605771463229782	14.110975987693655
7	27.75422493650961	30.6025220234692	22.188242489825686	19.45501055019551
8	26.21920473106974	34.02911097087369	20.316822933660085	19.43486136439649
9	27.083983238332138	15.622062642489007	19.96733172617154	37.32662239300731
10-11	26.511981605941248	24.865322637559107	27.210094639805178	21.412601116694468
12-13	26.86162623362623	22.788166597968512	27.43505978785055	22.915147380554707
14-15	23.790563021436892	24.81285293037689	24.750615470688047	26.64596857749817
16-17	23.415757481535877	27.432707344838487	25.948929485009316	23.202605688616323
18-19	23.49758158630346	26.78982558169267	26.657168251838232	23.05542458016563
20-21	24.667638334867547	25.388002454740715	26.35593740411169	23.588421806280042
22-23	24.825456635716286	25.878700288638367	25.65782340896027	23.63801966668508
24-25	24.38707196294995	25.48561451849715	25.943421555285877	24.18389196326702
26-27	23.798922965985977	26.97709384924592	26.0796677866593	23.144315398108798
28-29	23.659398758485676	26.68069311234692	26.58960986092544	23.070298268241967
30-31	23.801112408944274	25.688914163678355	26.570372446297323	23.939600981080048
32-33	23.717291436642796	26.11259104854064	26.914774525892998	23.255342988923566
34-35	24.477884134950017	25.643142110331546	25.95029766881921	23.928676085899227
36-37	23.280250917081887	25.83416991112245	26.668173614737135	24.217405557058527
38-39	23.667724584154556	26.01991189589519	26.0383223194679	24.27404120048235
40-41	23.842623608095266	25.977823622005364	26.484826231171503	23.69472653872786
42-43	23.90736693099262	25.957981276599227	26.60469854465602	23.52995324775214
44-45	24.097600693932996	25.519631429927497	26.326543346262742	24.05622452987676
46-47	23.868094342915604	25.43761013506148	26.240281372530454	24.454014149492465
48-49	23.515495550782447	25.466093893832465	26.660274112713513	24.358136442671576
50-51	23.908134031974814	25.64311189344047	26.393183233831813	24.055570840752903
52-53	24.028262045786725	25.985034371238008	25.708417757058093	24.27828582591717
54-55	24.173448691683706	25.267897233015617	26.11048095185981	24.448173123440867
56-57	24.395858425454982	25.46076167534822	25.93537036610844	24.208009533088358
58-59	24.33996366711465	25.6886084484446	26.19359565110403	23.777832233336728
60-61	23.951917228636656	26.295182463978588	25.77305296133418	23.979847346050573
62-63	23.774249340548856	25.631451958511086	26.48733209438001	24.106966606560043
64-65	24.084464977748958	25.284210913903653	26.04936857361152	24.581955534735865
66-67	23.780181588144504	25.638151307088936	25.834120038007296	24.747547066759264
68-69	24.076845107992476	25.57294772360226	26.485337631826305	23.864869536578954
70-71	24.409613514069143	25.506567918609562	26.208363037188033	23.87545553013326
72-73	23.84364640940486	25.690621014271148	26.411286817011643	24.05444575931235
74-75	24.08819820252898	25.333305376764205	26.337747402851775	24.24074901785504
76-77	24.088388579178496	24.844495607317334	26.085155499278667	24.9819603142255
78-79	24.109537898179568	24.641526513766397	26.142264250334346	25.10667133771969
80-81	23.49399716159718	25.765537698336594	26.075920882717707	24.664544257348524
82-83	23.29573320255027	25.716566576369758	26.581299692492742	24.40640052858723
84-85	23.782514107217924	25.03572192032043	26.658064766884426	24.523699205577223
86-87	23.483773768623934	26.091354567370388	26.164842841464775	24.260028822540907
88-89	23.99747572636792	25.039863681041457	25.633906681654118	25.32875391093651
90-91	23.909463673677287	25.100566938765905	25.77254739916969	25.217421988387112
92-93	23.90097442280765	25.374268569213477	26.32649658844623	24.39826041953264
94-95	23.817820676337593	24.967091367863787	26.295914829289345	24.91917312650927
96-97	24.181119701505665	25.540729482350027	26.111043492580087	24.167107323564217
98-99	22.604573762896248	25.960078019283895	26.52742590571613	24.90792231210373
100-101	23.44274039617843	25.401167759202114	26.103614322193053	25.052477522426404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	3.5
4	2.5
5	2.5
6	5.0
7	9.0
8	15.5
9	16.0
10	20.0
11	28.0
12	26.5
13	23.0
14	24.5
15	27.5
16	35.0
17	49.5
18	57.5
19	70.0
20	84.0
21	91.5
22	112.0
23	143.0
24	173.5
25	231.5
26	311.5
27	413.5
28	544.0
29	785.5
30	1240.5
31	2230.0
32	4079.5
33	6979.5
34	10628.5
35	15591.0
36	22252.5
37	28625.5
38	33031.0
39	35387.0
40	35423.0
41	34181.5
42	32978.0
43	32434.5
44	32291.5
45	32087.0
46	32089.0
47	32793.0
48	34695.0
49	38908.0
50	43888.0
51	43468.5
52	40663.5
53	41731.0
54	45301.5
55	45197.0
56	37699.5
57	31664.5
58	30582.0
59	29757.5
60	24657.5
61	18111.5
62	14650.0
63	10220.0
64	5850.0
65	3483.0
66	2577.5
67	2328.5
68	1733.5
69	994.5
70	560.5
71	375.0
72	276.0
73	217.5
74	164.5
75	123.0
76	79.0
77	43.5
78	33.5
79	16.0
80	5.5
81	2.0
82	3.5
83	4.5
84	3.0
85	2.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4816371366881898
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.159609167163577E-4
22-23	0.001329641702473236
24-25	2.557003273986992E-4
26-27	0.0022501628811085533
28-29	0.0030684039287843905
30-31	9.205211786353171E-4
32-33	5.1140065479739843E-5
34-35	0.0027104234704262114
36-37	0.0013807817679529757
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.00930749191731265
46-47	0.07993192234483337
48-49	7.159609167163577E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.006085667792089041
58-59	0.036360586556095024
60-61	0.027359935031660815
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.5342019643921952E-4
78-79	0.005574267137291642
80-81	0.004551465827696845
82-83	6.64820851236618E-4
84-85	0.001636482095351675
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06361824145679636
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	977707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.26294953623794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.19641230323174	29.244444812601973
2	16.0004814346463	13.524550788559372
3	6.166296914352396	7.818176859502052
4	2.9935401706357485	5.06063348665119
5	1.610963746337681	3.404203975808911
6	0.9628023764816869	2.44145209503693
7	0.650721930445916	1.925099967599136
8	0.43658946307688656	1.476124675685734
9	0.30002544386133884	1.1411964174149238
>10	1.4036923999181206	10.476801613190087
>50	0.12492679492981908	3.71284073753176
>100	0.1338988249597697	12.414751988955095
>500	0.014554220090888434	4.2199228391245756
>1k	0.005093977031810952	3.1397997423382256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2207	0.22573224902757166	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1991	0.20363974074032407	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1968	0.20128729772825601	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1955	0.19995765602578278	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1856	0.18983192306079427	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1736	0.17755830734565672	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1567	0.16027296521350465	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1519	0.15536351892744962	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1493	0.15270423552250317	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1409	0.14411270452190686	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1315	0.1344983722117158	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1313	0.13429381194979684	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1312	0.13419153181883733	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1236	0.12641824186591688	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1234	0.12621368160399793	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1175	0.12017915387738862	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1116	0.11414462615077932	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1107	0.11322410497214401	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	1075	0.10995114078144066	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1040	0.10637133619785885	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1003	0.10258697135235811	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.0456026191895937E-4	0.0
18-19	0.0	0.0	0.0	2.0456026191895937E-4	0.0
20-21	0.0	0.0	0.0	2.557003273986992E-4	0.0
22-23	0.0	0.0	0.0	4.6026058931765857E-4	0.0
24-25	0.0	0.0	0.0	0.001022801309594797	0.0
26-27	0.0	0.0	0.0	0.001994462553709854	0.0
28-29	0.0	0.0	0.0	0.003528664518102049	0.0
30-31	0.0	0.0	0.0	0.008233550542238115	0.0
32-33	0.0	0.0	0.0	0.019177524554902438	0.0
34-35	0.0	0.0	0.0	0.03421270380594595	0.0
36-37	0.0	0.0	0.0	0.05988501667677536	0.0
38-39	0.0	0.0	0.0	0.10524625475730459	0.0
40-41	0.0	0.0	0.0	0.15551693912388886	0.0
42-43	0.0	0.0	0.0	0.20548078309759468	0.0
44-45	0.0	0.0	0.0	0.26526351964341055	0.0
46-47	0.0	0.0	0.0	0.3376778523627222	0.0
48-49	0.0	0.0	0.0	0.41121726652258805	0.0
50-51	0.0	0.0	0.0	0.488285345200556	0.0
52-53	0.0	0.0	0.0	0.5701605900336195	0.0
54-55	0.0	0.0	0.0	0.656740720890819	0.0
56-57	0.0	0.0	0.0	0.7548785065464398	0.0
58-59	0.0	0.0	0.0	0.8581814388155142	0.0
60-61	0.0	0.0	0.0	0.9747296480438413	0.0
62-63	0.0	0.0	0.0	1.1056482156719754	0.0
64-65	0.0	0.0	0.0	1.228793493347189	0.0
66-67	0.0	0.0	0.0	1.3722925170833389	0.0
68-69	0.0	0.0	0.0	1.5404410523807233	0.0
70-71	0.0	0.0	0.0	1.7137547342915618	0.0
72-73	0.0	0.0	0.0	1.9017456149950855	0.0
74-75	0.0	0.0	0.0	2.0946970820501436	0.0
76-77	0.0	0.0	0.0	2.300126725082259	0.0
78-79	0.0	0.0	0.0	2.5310752607887643	0.0
80-81	0.0	0.0	0.0	2.791685034473518	0.0
82-83	0.0	0.0	0.0	3.0739270558562026	0.0
84-85	0.0	0.0	0.0	3.360771683132063	0.0
86-87	0.0	0.0	0.0	3.6652084929329543	0.0
88-89	0.0	0.0	0.0	3.999459960908534	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	850	0.0	49.43572	1
GTATCAA	1835	0.0	47.620304	1
CCGTGCG	195	0.0	43.833855	9
AAACCGT	205	0.0	39.379196	6
ATCAACG	2285	0.0	36.783993	3
AACCGTG	235	0.0	36.372776	7
TCAACGC	2360	0.0	35.61501	4
TCGGCGC	55	0.0025829882	34.535767	7
TGCGCCG	55	0.0025829882	34.535767	5
ACCGTGC	255	0.0	33.520008	8
ACGCAGA	2755	0.0	30.508682	7
CGCAGAG	2770	0.0	30.343473	8
TATCACG	80	4.5005415E-4	29.679174	2
TCGCACC	950	0.0	29.655518	1
CAACGCA	2840	0.0	29.595572	5
AACGCAG	2885	0.0	29.133942	6
CGCACCA	990	0.0	28.779804	2
GTACATG	5020	0.0	28.25074	1
TACATGG	5120	0.0	27.453236	2
TTGCGCC	70	0.008402456	27.135244	4
>>END_MODULE