##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224499_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12309
Sequences flagged as poor quality	0
Sequence length	101
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973109107157363	34.0	31.0	34.0	30.0	34.0
2	32.121862052156956	34.0	31.0	34.0	30.0	34.0
3	32.27516451377041	34.0	31.0	34.0	31.0	34.0
4	35.493947518076205	37.0	37.0	37.0	35.0	37.0
5	35.47396214152246	37.0	37.0	37.0	35.0	37.0
6	35.54399220082866	37.0	37.0	37.0	35.0	37.0
7	35.52953123730604	37.0	37.0	37.0	35.0	37.0
8	35.563165163701356	37.0	37.0	37.0	35.0	37.0
9	37.343163538873995	39.0	39.0	39.0	35.0	39.0
10-11	37.36704850109676	39.0	39.0	39.0	35.0	39.0
12-13	37.38020960272971	39.0	39.0	39.0	35.0	39.0
14-15	38.914534080753924	41.0	40.0	41.0	36.0	41.0
16-17	38.72503858964985	41.0	40.0	41.0	35.5	41.0
18-19	38.78064830611748	41.0	40.0	41.0	35.5	41.0
20-21	38.83101795434235	41.0	40.0	41.0	36.0	41.0
22-23	38.780810788853685	41.0	40.0	41.0	35.5	41.0
24-25	38.7102526606548	41.0	39.5	41.0	35.0	41.0
26-27	38.5210415143391	41.0	39.0	41.0	35.0	41.0
28-29	38.417133804533265	41.0	39.0	41.0	34.0	41.0
30-31	38.25083272402307	41.0	39.0	41.0	34.0	41.0
32-33	37.99618165569908	40.5	38.0	41.0	33.0	41.0
34-35	37.9220489073036	40.0	38.0	41.0	33.0	41.0
36-37	37.863311398163944	40.0	38.0	41.0	33.0	41.0
38-39	37.69571045576407	40.0	37.5	41.0	33.0	41.0
40-41	37.50893655049151	40.0	37.0	41.0	32.5	41.0
42-43	37.25062962060281	40.0	36.0	41.0	31.5	41.0
44-45	36.968843935331876	40.0	35.0	41.0	30.5	41.0
46-47	36.74953286213339	40.0	35.0	41.0	30.0	41.0
48-49	36.50040620684052	39.0	35.0	41.0	30.0	41.0
50-51	35.19164838735884	38.0	34.0	40.0	28.0	40.5
52-53	35.48586400194979	38.0	34.5	40.0	28.5	40.5
54-55	35.958607522950686	38.0	35.0	41.0	29.0	41.0
56-57	35.819359818019336	37.5	35.0	41.0	29.0	41.0
58-59	35.648021772686654	37.0	35.0	41.0	28.5	41.0
60-61	35.486067105370054	36.5	35.0	41.0	28.0	41.0
62-63	35.25964741246243	36.0	35.0	40.5	27.5	41.0
64-65	35.05536599236331	35.5	34.5	40.0	27.5	41.0
66-67	34.858436916077665	35.0	34.0	40.0	27.0	41.0
68-69	34.563733853278094	35.0	34.0	39.5	26.0	41.0
70-71	34.356933950767726	35.0	34.0	39.0	26.0	41.0
72-73	34.125152327565196	35.0	34.0	39.0	26.0	41.0
74-75	33.84791615890811	35.0	34.0	38.5	26.0	40.5
76-77	33.50259972377935	35.0	33.0	37.0	25.0	39.5
78-79	33.27467706556179	35.0	33.0	37.0	25.0	39.0
80-81	33.00881468843936	35.0	33.0	36.0	24.0	39.0
82-83	32.71342107401089	35.0	33.0	36.0	24.0	38.0
84-85	32.469940693801284	35.0	33.0	35.5	24.0	37.0
86-87	32.21642700463076	35.0	33.0	35.0	23.5	37.0
88-89	32.06275895686083	35.0	33.0	35.0	24.0	36.0
90-91	31.861239743277274	35.0	33.0	35.0	23.0	36.0
92-93	31.64481273864652	35.0	32.0	35.0	23.0	36.0
94-95	31.57376716223901	35.0	32.0	35.0	22.0	35.5
96-97	31.340320090990332	35.0	31.5	35.0	20.0	35.0
98-99	31.191526525306685	35.0	31.0	35.0	20.0	35.0
100-101	29.739458932488425	34.0	28.0	35.0	15.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	6.0
10	19.0
11	50.0
12	66.0
13	89.0
14	46.0
15	56.0
16	40.0
17	27.0
18	49.0
19	40.0
20	55.0
21	39.0
22	48.0
23	61.0
24	78.0
25	66.0
26	65.0
27	89.0
28	113.0
29	123.0
30	162.0
31	180.0
32	296.0
33	396.0
34	638.0
35	1158.0
36	1948.0
37	2137.0
38	2969.0
39	1175.0
40	18.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.118125610152944	19.720143182557763	10.592255125284739	25.569476082004556
2	18.977983589243642	20.082866195466732	34.86067105370054	26.07847916158908
3	19.99350069055163	23.308148509220892	27.50020310342026	29.198147696807215
4	15.04590137297912	16.662604598261435	34.324478024209924	33.967016004549514
5	17.255666585425296	34.8931676009424	31.318547404338286	16.532618409294013
6	32.48842310504509	33.642050532130966	17.88122511983102	15.988301242992934
7	28.190754732309692	29.726216589487365	22.18701762937688	19.89601104882606
8	25.97286538305305	33.317085059712404	20.37533512064343	20.33471443659111
9	25.940368835811196	14.322853196847834	20.115362742708587	39.621415224632386
10-11	27.483954829799334	23.438134698188318	26.379072223576244	22.698838248436104
12-13	26.74872044845235	21.602079779023477	26.289706718661144	25.359493053863027
14-15	25.413399423069112	22.760329906959736	22.97160037378621	28.854670296184942
16-17	24.968504896980534	25.594343073109275	24.493030438493115	24.944121591417076
18-19	24.892337693995287	24.85577313723897	25.86739254083042	24.38449662793532
20-21	26.1072734660707	23.917106867127185	24.66070702966274	25.314912637139376
22-23	25.488682082334297	24.66777746169789	24.736863494127686	25.106676961840122
24-25	25.213293247745185	24.632323068172585	24.356057528235965	25.79832615584627
26-27	24.997968965797384	25.55041026890893	24.287106994881793	25.164513770411894
28-29	24.957348281745066	24.957348281745066	24.44958973109107	25.6357137054188
30-31	25.570720610935087	24.920789666097978	24.096189779835893	25.412299943131046
32-33	25.497603379640914	24.3642862945812	24.67300349337883	25.46510683239906
34-35	25.38386546429442	24.319603542123648	24.977658623771223	25.31887236981071
36-37	25.70592776175192	24.14577662211027	24.466745215942794	25.681550400195018
38-39	24.7430637364423	24.799935004265343	25.068042409716863	25.3889588495755
40-41	25.268118297042573	23.805654858628532	25.381865453363666	25.544361390965225
42-43	25.80225851003331	24.44552766268584	24.453651799496303	25.29856202778455
44-45	25.654046148846277	24.252518687032822	24.735944101397465	25.35749106272343
46-47	25.595093021366477	24.417093183849218	24.27898285807133	25.708830936712975
48-49	25.432610285157203	24.242424242424242	24.534893167600945	25.790072304817613
50-51	24.96953448696076	24.82736209277764	24.835486229588106	25.367617190673492
52-53	25.790072304817613	24.209927695182387	24.242424242424242	25.757575757575758
54-55	25.64383784222926	24.478024209927696	24.04744495897311	25.83069298886993
56-57	24.70143797221545	24.79486554553579	24.453651799496303	26.05004468275246
58-59	25.492585821653464	24.200690635791183	24.9197643713183	25.38695917123705
60-61	25.06805346767968	24.21890870678097	24.21890870678097	26.49412911875838
62-63	25.211279050869496	24.63838777831952	24.38647814074435	25.763855030066633
64-65	25.558715969118246	23.91304347826087	24.38033319788704	26.147907354733846
66-67	25.559663592410516	24.01576402714013	24.14171372851745	26.282858651931907
68-69	25.0385896498497	24.25867251604517	25.07514826549679	25.627589568608332
70-71	25.257941343732227	24.46177593630677	24.205865626777154	26.07441709318385
72-73	24.81923795596718	24.473962141522463	24.888293118856122	25.81850678365424
74-75	24.997968965797384	24.534893167600945	24.38053456820213	26.086603298399545
76-77	25.78194816800715	24.453651799496303	24.311479405313186	25.45292062718336
78-79	25.70383912248629	23.53443022547227	24.822262847856997	25.939467804184442
80-81	25.638003901170354	23.89873211963589	24.195383615084527	26.26788036410923
82-83	26.050556774770385	23.323579614728114	24.689100219458666	25.936763391042835
84-85	25.724269635528827	24.1355491446914	24.452480598106536	25.687700621673237
86-87	24.84252448490267	24.781566220994026	24.671841345958466	25.70406794814484
88-89	25.445902571811647	23.828870921870557	24.401738918457724	26.323487587860072
90-91	25.28231375416362	23.482817450645868	24.689251766999757	26.545617028190755
92-93	24.957348281745066	23.79965878625396	24.981720692176456	26.261272239824518
94-95	25.936306767405963	23.657486392070844	24.352100089365507	26.05410675115769
96-97	25.249817206921765	23.5762450239662	24.664879356568363	26.509058412543666
98-99	25.948492972621658	23.259403688358113	24.664879356568363	26.127223982451863
100-101	26.163097801795942	23.379789525009144	24.115233025882734	26.341879647312176
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.5
12	1.5
13	1.5
14	1.0
15	1.0
16	1.0
17	0.5
18	2.5
19	4.0
20	3.0
21	5.5
22	11.5
23	15.0
24	17.0
25	31.5
26	41.5
27	50.0
28	70.0
29	86.5
30	113.0
31	148.0
32	187.5
33	220.0
34	256.5
35	264.0
36	273.5
37	293.0
38	300.5
39	315.0
40	331.0
41	343.5
42	305.0
43	283.0
44	297.5
45	284.5
46	269.5
47	256.5
48	249.5
49	260.5
50	263.5
51	259.5
52	259.0
53	265.5
54	257.0
55	276.5
56	299.0
57	294.0
58	304.5
59	323.0
60	328.0
61	325.5
62	323.5
63	295.0
64	282.5
65	286.5
66	260.5
67	235.5
68	213.5
69	180.0
70	164.0
71	155.0
72	157.0
73	160.0
74	122.0
75	87.0
76	71.5
77	60.0
78	49.5
79	39.0
80	34.5
81	24.0
82	15.5
83	15.0
84	9.0
85	3.0
86	2.0
87	3.0
88	2.5
89	0.5
90	1.0
91	1.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1381103257778861
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.02031034202615972
16-17	0.044682752457551385
18-19	0.016248273620927775
20-21	0.03249654724185555
22-23	0.044682752457551385
24-25	0.016248273620927775
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02031034202615972
38-39	0.004062068405231944
40-41	0.008124136810463888
42-43	0.0
44-45	0.008124136810463888
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.012186205215695833
60-61	0.02031034202615972
62-63	0.024372410431391666
64-65	0.03249654724185555
66-67	0.02031034202615972
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.012186205215695833
80-81	0.04062068405231944
82-83	0.04874482086278333
84-85	0.028434478836623606
86-87	0.044682752457551385
88-89	0.02031034202615972
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.028434478836623606
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	12309.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.87220732797141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.8970144246897	93.86627670809976
2	2.9855753102985574	5.784385409050288
3	0.10902381751090237	0.31684133560809163
4	0.008386447500838645	0.03249654724185555
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.012186205215695831	0.0
34-35	0.0	0.0	0.0	0.0649930944837111	0.0
36-37	0.0	0.0	0.0	0.10967584694126249	0.0
38-39	0.0	0.0	0.0	0.1665448046145097	0.0
40-41	0.0	0.0	0.0	0.3330896092290194	0.0
42-43	0.0	0.0	0.0	0.4752620034121374	0.0
44-45	0.0	0.0	0.0	0.6336826712161833	0.0
46-47	0.0	0.0	0.0	0.7880412706149972	0.0
48-49	0.0	0.0	0.0	0.9952067592818263	0.0
50-51	0.0	0.0	0.0	1.1373791534649444	0.0
52-53	0.0	0.0	0.0	1.2998618896742222	0.0
54-55	0.0	0.0	0.0	1.5354618571776748	0.0
56-57	0.0	0.0	0.0	1.8116825087334472	0.0
58-59	0.0	0.0	0.0	2.0472824762368997	0.0
60-61	0.0	0.0	0.0	2.388496222276383	0.0
62-63	0.0	0.0	0.0	2.640344463400764	0.0
64-65	0.0	0.0	0.0	2.977496141035015	0.0
66-67	0.0	0.0	0.0	3.3146478186692665	0.0
68-69	0.0	0.0	0.0	3.631489154277358	0.0
70-71	0.0	0.0	0.0	3.9767649687220734	0.0
72-73	0.0	0.0	0.0	4.297668372735396	0.0
74-75	0.0	0.0	0.0	4.683564871232432	0.0
76-77	0.0	0.0	0.0	5.093833780160858	0.0
78-79	0.0	0.0	0.0	5.532537167925907	0.0
80-81	0.0	0.0	0.0	6.019985376553741	0.0
82-83	0.0	0.0	0.0	6.572426679665286	0.0
84-85	0.0	0.0	0.0	7.063936956698351	0.0
86-87	0.0	0.0	0.0	7.510764481273865	0.0
88-89	0.0	0.0	0.0	8.063205784385408	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGGAA	20	5.2865077E-4	47.497967	22-23
>>END_MODULE