##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12309
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15184011698757	34.0	33.0	34.0	31.0	34.0
2	33.28596961572833	34.0	34.0	34.0	31.0	34.0
3	33.379640913152976	34.0	34.0	34.0	31.0	34.0
4	36.58737509139654	37.0	37.0	37.0	35.0	37.0
5	36.62425867251604	37.0	37.0	37.0	35.0	37.0
6	36.64984970346901	37.0	37.0	37.0	35.0	37.0
7	36.62198391420912	37.0	37.0	37.0	35.0	37.0
8	36.59931757250792	37.0	37.0	37.0	35.0	37.0
9	38.46697538386547	39.0	39.0	39.0	37.0	39.0
10-11	38.49809082784954	39.0	39.0	39.0	37.0	39.0
12-13	38.513567308473476	39.0	39.0	39.0	37.0	39.0
14-15	40.13676984320416	41.0	40.0	41.0	38.0	41.0
16-17	40.13977577382403	41.0	40.0	41.0	38.0	41.0
18-19	40.07998212689902	41.0	40.0	41.0	38.0	41.0
20-21	40.07234543829718	41.0	40.0	41.0	38.0	41.0
22-23	39.96551303923958	41.0	40.0	41.0	38.0	41.0
24-25	39.93378828499472	41.0	40.0	41.0	38.0	41.0
26-27	39.86440815663336	41.0	40.0	41.0	38.0	41.0
28-29	39.784466650418395	41.0	40.0	41.0	38.0	41.0
30-31	39.705703144040946	41.0	40.0	41.0	37.0	41.0
32-33	39.59505240068243	41.0	40.0	41.0	37.0	41.0
34-35	39.452961247867414	41.0	39.0	41.0	36.0	41.0
36-37	39.297221545210824	41.0	39.0	41.0	35.0	41.0
38-39	39.121293362580225	41.0	39.0	41.0	35.0	41.0
40-41	38.86826712161833	40.5	38.0	41.0	35.0	41.0
42-43	38.69530424892355	40.0	37.5	41.0	35.0	41.0
44-45	38.37403525875376	40.0	36.5	41.0	35.0	41.0
46-47	38.06860833536437	40.0	35.5	41.0	34.5	41.0
48-49	37.969006418068076	40.0	35.0	41.0	34.0	41.0
50-51	37.76565927370217	39.5	35.0	41.0	34.0	41.0
52-53	37.53469006418068	39.0	35.0	41.0	33.5	41.0
54-55	37.35474043382891	39.0	35.0	41.0	33.0	41.0
56-57	37.144934600698676	38.5	35.0	41.0	33.0	41.0
58-59	36.909740840035745	37.5	35.0	41.0	33.0	41.0
60-61	36.67479080347713	37.0	35.0	41.0	33.0	41.0
62-63	36.503818344300925	36.5	35.0	41.0	33.0	41.0
64-65	36.30055244130311	36.0	35.0	40.0	33.0	41.0
66-67	36.00312779267203	35.5	35.0	40.0	32.0	41.0
68-69	35.847388090015436	35.0	35.0	39.5	32.0	41.0
70-71	35.588187505077585	35.0	35.0	39.0	32.0	41.0
72-73	35.33954829799334	35.0	35.0	39.0	31.0	41.0
74-75	35.0533349581607	35.0	35.0	38.0	31.0	40.5
76-77	33.07640750670241	34.5	32.0	35.5	27.5	38.5
78-79	34.53789909822081	35.0	34.0	37.0	31.0	39.0
80-81	34.50410268908928	35.0	35.0	36.0	31.0	39.0
82-83	34.30270533755788	35.0	35.0	36.0	31.0	38.0
84-85	34.13071736128036	35.0	34.0	36.0	31.0	37.0
86-87	33.88020960272971	35.0	34.0	35.0	31.0	37.0
88-89	33.71650824599887	35.0	34.0	35.0	31.0	36.5
90-91	33.6042732959623	35.0	34.0	35.0	30.0	36.0
92-93	33.48744820862784	35.0	34.0	35.0	30.0	36.0
94-95	33.35868064018199	35.0	34.0	35.0	30.0	36.0
96-97	33.27163051425786	35.0	34.0	35.0	30.0	35.5
98-99	33.15078397920221	35.0	34.0	35.0	29.0	35.0
100-101	32.02416930701113	34.5	32.5	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	4.0
17	2.0
18	2.0
19	8.0
20	18.0
21	19.0
22	25.0
23	31.0
24	32.0
25	38.0
26	52.0
27	59.0
28	77.0
29	78.0
30	112.0
31	154.0
32	180.0
33	267.0
34	498.0
35	1076.0
36	2366.0
37	2501.0
38	3295.0
39	1394.0
40	20.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.1052373959863	18.534834393865232	10.899004731603851	25.460923478544622
2	18.831749126655293	19.416686977008695	35.673084734746936	26.07847916158908
3	19.44918352425055	23.072548541717442	28.28824437403526	29.190023559996753
4	14.712811763750103	17.255666585425296	34.18636769843204	33.84515395239256
5	16.613859777398652	32.72402307254854	33.983264278170445	16.678852871882363
6	33.585181574457714	33.57705743764725	17.523763100170605	15.31399788772443
7	29.409375253879276	28.43447883662361	22.081403850840847	20.074742058656266
8	26.435941181249493	32.41530587375092	20.58656267771549	20.5621902672841
9	25.63977577382403	14.200991144690878	20.45657648874807	39.70265659273702
10-11	26.651230806726783	23.42188642456739	27.053375578844747	22.87350718986108
12-13	26.419692907628566	22.17076935575595	26.509058412543666	24.900479324071817
14-15	24.640506946136973	22.824762368998293	23.092858883743602	29.441871801121135
16-17	25.46916890080429	25.980989519863513	24.25867251604517	24.291169063287025
18-19	25.168575838817127	25.083272402307255	25.31887236981071	24.429279389064913
20-21	25.314810301405476	24.61613453570558	24.388658705012592	25.680396457876352
22-23	25.84392899215989	25.177722711947027	24.539952065645693	24.43839623024739
24-25	25.509789584856605	24.079941506214965	24.888293118856122	25.521975790072304
26-27	24.82330002437241	25.209196522869444	24.831424161182873	25.13607929157527
28-29	25.456982695588593	24.478024209927696	25.160451702006664	24.90454139247705
30-31	25.582906816150786	24.583637988463725	24.408969047038752	25.424486148346737
32-33	24.90454139247705	24.965472418555528	24.786741408725323	25.343244780242095
34-35	25.11576894954911	24.733934519457307	24.77861727191486	25.37167925907872
36-37	25.640817321363286	24.702441402282975	24.288093593857905	25.368647682495837
38-39	25.257941343732227	24.888293118856122	24.798927613941018	25.05483792347063
40-41	25.71695507352344	24.209927695182387	24.498334551953853	25.57478267934032
42-43	24.88423105045089	24.473962141522463	25.103582744333412	25.53822406369323
44-45	25.254946572949255	24.722707512290253	24.787713809775322	25.23463210498517
46-47	25.293711126468555	24.52945241676491	25.440058538964998	24.736777917801536
48-49	25.11983101795434	24.29929320009749	25.002031034202616	25.57884474774555
50-51	25.13607929157527	24.595824193679423	24.90860346088228	25.359493053863027
52-53	25.099520675928183	24.67706556178406	24.798927613941018	25.424486148346737
54-55	25.29449995937932	24.551141441221873	24.421155252254447	25.733203347144368
56-57	24.938050940407035	24.978673274566358	24.873055205752124	25.210220579274484
58-59	26.0330746414205	24.111169802121	24.769412051521677	25.086343504936814
60-61	25.331112375071097	23.67758186397985	24.729828552856098	26.261477208092955
62-63	25.245755138516536	24.486148346738158	25.123893086359573	25.144203428385737
64-65	25.46916890080429	23.937769112031845	24.413031115443985	26.18003087171988
66-67	24.88016898204566	24.06775530099927	24.807051750751484	26.24502396620359
68-69	24.88016898204566	23.986513932894628	25.002031034202616	26.131286050857096
70-71	24.786741408725323	24.372410431391664	24.937037939718902	25.903810220164107
72-73	25.176699975627592	23.685920870907466	25.01421723941831	26.123161914046634
74-75	24.78267934032009	24.063693232594037	24.587700056868957	26.56592737021691
76-77	24.8476724348038	24.13681046388821	24.502396620359086	26.5131204809489
78-79	25.270127548947922	23.884962222763832	24.270858721260865	26.57405150702738
80-81	25.01421723941831	23.897148427979527	24.494272483548624	26.594361849053538
82-83	25.12795515476481	23.86058981233244	24.82736209277764	26.184092940125115
84-85	24.900479324071817	24.059631164188804	24.388658705012592	26.651230806726783
86-87	24.900479324071817	24.388658705012592	24.315541473718415	26.395320497197172
88-89	25.03046551303924	23.5762450239662	24.82330002437241	26.569989438622148
90-91	25.091396539117717	23.998700138110326	24.522706962385246	26.38719636038671
92-93	24.86798277682996	23.917458770005688	24.9492241449346	26.265334308229747
94-95	24.98578276058169	23.978389796084166	24.534893167600945	26.5009342757332
96-97	24.961410350150295	24.039320822162647	24.392720773417825	26.606548054269236
98-99	24.872044845235195	23.84027947030628	24.563327646437568	26.724348038020963
100-101	24.70731707317073	24.020325203252032	24.520325203252032	26.7520325203252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	1.0
16	1.5
17	0.5
18	2.0
19	4.5
20	5.5
21	4.5
22	8.5
23	15.0
24	25.5
25	33.5
26	38.5
27	49.0
28	65.5
29	92.5
30	110.5
31	135.5
32	187.5
33	218.0
34	233.5
35	268.0
36	300.5
37	313.0
38	309.0
39	319.5
40	321.0
41	318.0
42	327.5
43	311.5
44	288.5
45	274.0
46	264.0
47	256.0
48	265.5
49	278.5
50	267.0
51	268.5
52	261.0
53	257.5
54	270.5
55	273.0
56	296.0
57	315.5
58	317.0
59	323.5
60	332.5
61	321.5
62	320.0
63	303.5
64	273.0
65	269.5
66	240.5
67	223.5
68	224.0
69	193.5
70	175.5
71	156.0
72	143.5
73	135.5
74	116.0
75	99.0
76	72.5
77	54.0
78	39.5
79	31.5
80	26.5
81	23.0
82	15.5
83	6.5
84	3.5
85	4.0
86	3.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.41433097733365826
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.004062068405231944
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.004062068405231944
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.02031034202615972
46-47	0.07717929969940694
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.004062068405231944
58-59	0.028434478836623606
60-61	0.016248273620927775
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.073117231294175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	12309.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.54724185555284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.60047122181084	93.26509058412543
2	3.2648939750925616	6.304330164919977
3	0.11780545270952542	0.3412137460394833
4	0.00841467519353753	0.03249654724185555
5	0.0	0.0
6	0.0	0.0
7	0.00841467519353753	0.05686895767324721
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.004062068405231944	0.0
30-31	0.0	0.0	0.0	0.008124136810463888	0.0
32-33	0.0	0.0	0.0	0.020310342026159722	0.0
34-35	0.0	0.0	0.0	0.073117231294175	0.0
36-37	0.0	0.0	0.0	0.11779998375172637	0.0
38-39	0.0	0.0	0.0	0.18279307823543747	0.0
40-41	0.0	0.0	0.0	0.34933788284994716	0.0
42-43	0.0	0.0	0.0	0.507758550653993	0.0
44-45	0.0	0.0	0.0	0.6783654236737346	0.0
46-47	0.0	0.0	0.0	0.8367860914777805	0.0
48-49	0.0	0.0	0.0	1.0439515801446095	0.0
50-51	0.0	0.0	0.0	1.1861239743277276	0.0
52-53	0.0	0.0	0.0	1.3567308473474693	0.0
54-55	0.0	0.0	0.0	1.5882687464456902	0.0
56-57	0.0	0.0	0.0	1.8563652611909984	0.0
58-59	0.0	0.0	0.0	2.091965228694451	0.0
60-61	0.0	0.0	0.0	2.4250548379234704	0.0
62-63	0.0	0.0	0.0	2.676903079047851	0.0
64-65	0.0	0.0	0.0	3.0221788934925664	0.0
66-67	0.0	0.0	0.0	3.3715167763425136	0.0
68-69	0.0	0.0	0.0	3.6842960435453733	0.0
70-71	0.0	0.0	0.0	4.0295718579900885	0.0
72-73	0.0	0.0	0.0	4.358599398813876	0.0
74-75	0.0	0.0	0.0	4.764806239337071	0.0
76-77	0.0	0.0	0.0	5.187261353481192	0.0
78-79	0.0	0.0	0.0	5.62190267284101	0.0
80-81	0.0	0.0	0.0	6.109350881468844	0.0
82-83	0.0	0.0	0.0	6.657730116175156	0.0
84-85	0.0	0.0	0.0	7.153302461613453	0.0
86-87	0.0	0.0	0.0	7.6204403282151265	0.0
88-89	0.0	0.0	0.0	8.193191973352832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTGT	20	0.0020282376	71.22561	6
>>END_MODULE