##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224498_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891035
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.961442592041838	33.0	31.0	34.0	30.0	34.0
2	32.13307894751609	34.0	31.0	34.0	30.0	34.0
3	32.26967178618124	34.0	31.0	34.0	30.0	34.0
4	35.595727440560694	37.0	35.0	37.0	35.0	37.0
5	35.600439937825115	37.0	35.0	37.0	35.0	37.0
6	35.702563872350694	37.0	37.0	37.0	35.0	37.0
7	35.65269041058993	37.0	37.0	37.0	35.0	37.0
8	35.69434309538907	37.0	37.0	37.0	35.0	37.0
9	37.390917304034076	39.0	38.0	39.0	35.0	39.0
10-11	37.4461373571184	39.0	38.0	39.0	35.0	39.0
12-13	37.41939991133906	39.0	38.0	39.0	35.0	39.0
14-15	38.83374558799598	41.0	39.0	41.0	35.0	41.0
16-17	38.673218784896214	41.0	39.0	41.0	34.5	41.0
18-19	38.75424758847857	41.0	39.0	41.0	35.0	41.0
20-21	38.80936663542958	41.0	39.0	41.0	35.0	41.0
22-23	38.754663397060725	41.0	39.0	41.0	35.0	41.0
24-25	38.66131408979445	41.0	39.0	41.0	35.0	41.0
26-27	38.45416790586228	41.0	39.0	41.0	34.0	41.0
28-29	38.37097364301066	40.0	38.5	41.0	34.0	41.0
30-31	38.263409405915596	40.0	38.0	41.0	34.0	41.0
32-33	38.05322798767725	40.0	38.0	41.0	33.0	41.0
34-35	38.019778684339	40.0	38.0	41.0	33.0	41.0
36-37	37.984820461598034	40.0	38.0	41.0	33.0	41.0
38-39	37.8358308035038	40.0	38.0	41.0	33.0	41.0
40-41	37.69836818980174	40.0	38.0	41.0	32.5	41.0
42-43	37.50663722524929	40.0	37.0	41.0	32.0	41.0
44-45	37.266469891755094	40.0	37.0	41.0	31.0	41.0
46-47	37.017037490109814	40.0	36.0	41.0	31.0	41.0
48-49	36.74018136212382	39.5	35.5	41.0	30.0	41.0
50-51	35.38937527706544	38.0	34.0	40.0	28.0	40.5
52-53	35.6892260124462	38.5	35.0	39.5	28.5	40.5
54-55	36.070667818884786	38.5	35.0	40.5	29.0	41.0
56-57	35.85899375445409	37.5	35.0	41.0	29.0	41.0
58-59	35.66634700095956	37.0	35.0	40.0	29.0	41.0
60-61	35.37243710965338	36.5	35.0	40.0	28.5	41.0
62-63	35.08632433069408	36.0	35.0	40.0	28.0	41.0
64-65	34.81443658217691	35.0	34.0	39.5	28.0	41.0
66-67	34.578937415477505	35.0	34.0	39.0	28.0	41.0
68-69	34.28706279775766	35.0	34.0	39.0	27.0	41.0
70-71	34.03067780726907	35.0	34.0	38.5	26.5	41.0
72-73	33.730698008495736	35.0	34.0	37.0	26.0	40.0
74-75	33.508292603545314	35.0	34.0	37.0	26.0	39.5
76-77	33.23434096303737	35.0	33.0	36.0	26.0	39.0
78-79	33.06514727255383	35.0	33.0	36.0	26.0	39.0
80-81	32.82710499587559	35.0	33.0	35.5	25.5	37.5
82-83	32.59858647527875	35.0	33.0	35.0	25.0	37.0
84-85	32.4063959328197	35.0	33.0	35.0	25.0	37.0
86-87	32.21654761036323	35.0	33.0	35.0	25.0	36.0
88-89	32.097112908022694	35.0	33.0	35.0	24.5	36.0
90-91	31.91856941646512	35.0	33.0	35.0	24.0	36.0
92-93	31.78850886889965	35.0	32.5	35.0	24.0	35.5
94-95	31.650610806533976	35.0	32.0	35.0	24.0	35.0
96-97	31.526067438428342	35.0	32.0	35.0	23.0	35.0
98-99	31.491982918740568	35.0	32.0	35.0	23.0	35.0
100-101	30.227562329201433	34.0	29.5	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	175.0
8	610.0
9	493.0
10	568.0
11	1279.0
12	2151.0
13	2615.0
14	2561.0
15	2609.0
16	2824.0
17	3106.0
18	3456.0
19	3960.0
20	4737.0
21	6461.0
22	7084.0
23	6666.0
24	6066.0
25	6257.0
26	6410.0
27	6596.0
28	7322.0
29	8343.0
30	10157.0
31	13026.0
32	17197.0
33	24813.0
34	41286.0
35	73576.0
36	157224.0
37	227937.0
38	184024.0
39	49437.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5206532612483	20.11475907338511	13.666220818487337	23.698366846879253
2	16.16670501158765	20.115034762944216	37.734320200665515	25.983940024802614
3	18.323073728865868	28.10013074682813	28.95666275735521	24.620132766950793
4	12.685696970377144	17.430067281307693	39.335491871811996	30.548743876503163
5	13.14942735133861	40.29606019965546	33.526741373795645	13.027771075210289
6	27.910463674266445	38.79847592967728	19.531780457557783	13.759279938498487
7	25.187226091006526	32.22050761193444	23.768202146941476	18.824064150117557
8	26.64878484010168	33.40665630418558	20.90176031244564	19.0427985432671
9	27.17199661068308	14.23771232330941	21.332607585560613	37.25768348044689
10-11	26.605015515664366	24.675068880571473	27.114310885655446	21.60560471810872
12-13	26.55726206041289	22.6620727580847	27.99239087129013	22.78827431021228
14-15	23.91384401208025	24.601273141651042	24.68474587688473	26.800136969383974
16-17	23.528189577717878	26.91071428571429	26.022742587601076	23.538353548966757
18-19	23.442976104678706	26.37595110922517	26.71665532856909	23.464417457527038
20-21	25.47960708539684	24.055016577277733	25.988096694985952	24.47727964233948
22-23	25.518690838361618	25.700618666918224	25.155115934070256	23.62557456064991
24-25	24.09496484924406	25.506383036042013	25.725549093758172	24.673103020955757
26-27	23.6917180582132	26.439589915098733	26.47943122324039	23.389260803447677
28-29	23.687060553176924	26.6602883163961	26.16356259855112	23.48908853187585
30-31	23.75540803672134	25.249906008181494	26.731553754903004	24.263132200194157
32-33	23.989854495053507	25.40652162934116	26.759835472231728	23.843788403373605
34-35	24.42047731009444	25.161918443158797	26.20879090046968	24.208813346277083
36-37	23.823031856914977	25.03325561528782	27.334544193204742	23.809168334592464
38-39	23.84027358454566	25.49181468620158	25.916737187789707	24.751174541463055
40-41	24.574077347632237	24.995019515083154	26.615486923070446	23.815416214214164
42-43	24.536688647475966	25.520241480261884	26.430786825431298	23.512283046830852
44-45	24.193845286987596	25.186519209766907	25.863454581576267	24.756180921669227
46-47	24.428613915278298	24.900368672386605	25.617231646343857	25.053785765991233
48-49	24.008765087791165	25.361237212904093	25.87984759296773	24.750150106337014
50-51	24.040357561711943	25.922214054442307	25.380989523419395	24.65643886042636
52-53	24.864511495059116	25.693435162479588	24.63735992413317	24.804693418328124
54-55	24.05012148793257	25.04542470273334	25.460391567110157	25.44406224222393
56-57	24.273345042562863	25.836358841122966	25.12224547857267	24.768050637741503
58-59	25.12265892904247	25.352262076474517	25.410967745556384	24.11411124892663
60-61	24.506898067104405	25.758153166779945	25.310274109840375	24.424674656275275
62-63	24.200511353909107	25.53396069142233	25.84936108826889	24.41616686639967
64-65	25.452257963462305	24.97219616343547	24.712993107567865	24.86255276553436
66-67	24.15030166970675	25.16166690051915	24.821158972919882	25.866872456854217
68-69	24.06212999489358	25.691583383368776	26.25957453972066	23.986712082016982
70-71	24.99329431503813	24.868383396836265	25.61930788352871	24.519014404596902
72-73	24.904689490311828	25.019555909700518	25.790344935945274	24.28540966404238
74-75	24.472271010678593	24.899751412683003	25.891407183780657	24.736570392857743
76-77	24.55144859629532	24.454202135718575	24.894308304387593	26.100040963598513
78-79	24.506121184467293	23.957282758705844	25.84266257633591	25.693933480490955
80-81	24.000406502010893	25.515019519395594	25.56701135117563	24.917562627417887
82-83	24.08815634861686	25.755572510426965	25.584620247372698	24.57165089358348
84-85	23.945893789225348	24.753512845397758	26.350115322028838	24.95047804334806
86-87	24.126659008194114	26.137850260017498	25.308444420488325	24.427046311300067
88-89	24.354975963859342	25.340053573313035	24.984507298409643	25.320463164417976
90-91	23.94069817683929	24.93061439786316	25.648431318635073	25.480256106662473
92-93	23.867762213510424	24.974438861460314	26.170621581793963	24.987177343235306
94-95	24.599763196731892	24.634610312726213	25.93371753073673	24.831908959805173
96-97	24.439949048017194	25.649665838042278	25.813351888534118	24.09703322540641
98-99	23.106849616398137	26.327455478391254	25.786563385213483	24.779131519997126
100-101	24.412300015831438	24.99418952587654	25.440894285300452	25.15261617299157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	2.5
2	1.5
3	2.5
4	5.5
5	6.0
6	4.5
7	15.5
8	28.5
9	38.0
10	43.5
11	46.0
12	45.5
13	59.5
14	71.5
15	76.5
16	99.0
17	116.0
18	129.0
19	147.5
20	179.0
21	229.5
22	288.5
23	374.5
24	552.0
25	838.0
26	1275.5
27	1908.5
28	2693.0
29	3856.0
30	5547.5
31	7374.0
32	8988.5
33	10850.0
34	12655.0
35	14272.5
36	16293.0
37	17479.0
38	18003.0
39	18892.0
40	19463.5
41	19690.0
42	20058.0
43	21075.5
44	22579.5
45	26007.0
46	30438.5
47	31626.0
48	34420.5
49	39945.0
50	45035.5
51	44421.0
52	41740.5
53	44226.5
54	46940.5
55	46973.5
56	39512.0
57	31734.5
58	29294.5
59	27200.0
60	22574.0
61	17003.5
62	13681.5
63	9940.5
64	6020.0
65	3967.0
66	3278.0
67	2727.5
68	2081.5
69	1354.0
70	719.0
71	428.5
72	306.5
73	261.0
74	210.0
75	162.5
76	114.0
77	77.5
78	52.5
79	38.5
80	31.5
81	22.0
82	16.5
83	13.0
84	8.0
85	6.5
86	7.0
87	9.0
88	7.0
89	3.0
90	3.5
91	5.5
92	5.5
93	4.5
94	5.0
95	5.5
96	4.5
97	2.5
98	1.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15094805479021586
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03647443703109306
16-17	0.0712654385069049
18-19	0.026149365625368255
20-21	0.040177995252711735
22-23	0.06419500917472377
24-25	0.017732187848962164
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023343639699899554
38-39	0.005499222813918646
40-41	0.006453169628578002
42-43	0.0034229856290718093
44-45	0.01212073599802477
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.01683435555281218
60-61	0.019022821774677765
62-63	0.029460122217421312
64-65	0.047192310066383476
66-67	0.017956645922999656
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.017227157182377797
80-81	0.05729292339807079
82-83	0.0488757456216647
84-85	0.0301334964395338
86-87	0.05813464117571139
88-89	0.03288310784649313
90-91	0.0
92-93	0.003815787258637427
94-95	0.0
96-97	0.0
98-99	7.85603259131235E-4
100-101	0.045116072881536635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	891035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.9596299800187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99088821747989	31.356615497358426
2	14.644370326033076	12.582334609934934
3	5.350541233760647	6.895718147855705
4	2.3960766863171266	4.117382713917324
5	1.2951524401673875	2.7819634798654636
6	0.7656688432855018	1.9735710116864449
7	0.5042057880169943	1.5162345860895674
8	0.33346886298156336	1.146055917083641
9	0.23499746570687044	0.9085863755708359
>10	1.1846186670484684	9.311706186775252
>50	0.12593098471498856	3.849698235269478
>100	0.15027008387428997	13.793276748434547
>500	0.01772552045645009	5.164892376083753
>1k	0.006084880156691822	4.601964114074624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	3854	0.4325307086702542	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	3565	0.4000965169718361	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	3104	0.34835893090619335	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2848	0.3196282974293939	No Hit
ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT	2197	0.24656719433018906	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1944	0.21817324796444582	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1817	0.20392016026306486	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1746	0.19595189863473375	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1734	0.19460515019050878	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1606	0.18023983345210906	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1600	0.1795664592299966	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1569	0.1760873590824154	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1462	0.16407885212140938	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1421	0.1594774616036407	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1326	0.14881570308685968	No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC	1220	0.1369194251628724	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1140	0.12794110220137256	No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC	1139	0.12782887316435382	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1087	0.12199296323937893	No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA	1062	0.11918723731391023	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1038	0.11649374042546029	No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA	1007	0.11301464027787911	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1005	0.1127901822038416	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC	988	0.11088228857452288	No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA	988	0.11088228857452288	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	980	0.10998445627837292	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	975	0.10942331109327916	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	932	0.10459746250147302	No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT	928	0.104148546353398	No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT	924	0.10369963020532302	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	911	0.1022406527240793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.683435555281218E-4	1.1222903701874786E-4
14-15	0.0	0.0	0.0	2.805725925468696E-4	1.683435555281218E-4
16-17	0.0	0.0	0.0	3.366871110562436E-4	2.2445807403749572E-4
18-19	0.0	0.0	0.0	3.366871110562436E-4	2.2445807403749572E-4
20-21	0.0	0.0	0.0	3.366871110562436E-4	2.2445807403749572E-4
22-23	0.0	0.0	0.0	4.4891614807499145E-4	2.2445807403749572E-4
24-25	0.0	0.0	0.0	4.4891614807499145E-4	2.2445807403749572E-4
26-27	0.0	0.0	0.0	7.294887406218611E-4	3.366871110562436E-4
28-29	0.0	0.0	0.0	0.0012906339257156003	3.366871110562436E-4
30-31	0.0	0.0	0.0	0.0029179549624874443	3.366871110562436E-4
32-33	0.0	0.0	0.0	0.0068459712581436195	3.366871110562436E-4
34-35	0.0	0.0	0.0	0.011447361775912282	5.611451850937393E-4
36-37	0.0	0.0	0.0	0.020313455700393362	5.611451850937393E-4
38-39	0.0	0.0	0.0	0.04505995836302727	7.294887406218611E-4
40-41	0.0	0.0	0.0	0.0738467063583361	7.85603259131235E-4
42-43	0.0	0.0	0.0	0.09954715583562934	7.85603259131235E-4
44-45	0.0	0.0	0.0	0.1242375439797539	7.85603259131235E-4
46-47	0.0	0.0	0.0	0.15813071315941574	7.85603259131235E-4
48-49	0.0	0.0	0.0	0.19729864707895872	7.85603259131235E-4
50-51	0.0	0.0	0.0	0.23450257285067366	7.85603259131235E-4
52-53	0.0	0.0	0.0	0.27456833906636663	7.85603259131235E-4
54-55	0.0	0.0	0.0	0.31547582305970023	7.85603259131235E-4
56-57	0.0	0.0	0.0	0.3631170492741587	7.85603259131235E-4
58-59	0.0	0.0	0.0	0.4190632242280045	8.417177776406089E-4
60-61	0.0	0.0	0.0	0.4877473948834782	8.978322961499829E-4
62-63	0.0	0.0	0.0	0.5590689479088924	8.978322961499829E-4
64-65	0.0	0.0	0.0	0.6218610941208819	0.0011222903701874785
66-67	0.0	0.0	0.0	0.6941927084794649	0.0012345194072062265
68-69	0.0	0.0	0.0	0.7840881671314819	0.0013467484442249743
70-71	0.0	0.0	0.0	0.8785289017827582	0.0014028629627343484
72-73	0.0	0.0	0.0	0.990140679097903	0.0014589774812437221
74-75	0.0	0.0	0.0	1.1004057079688228	0.0014589774812437221
76-77	0.0	0.0	0.0	1.2059571172849552	0.0014589774812437221
78-79	0.0	0.0	0.0	1.3304752338572559	0.0014589774812437221
80-81	0.0	0.0	0.0	1.478785906277531	0.001515091999753096
82-83	0.0	0.0	0.0	1.6380950243256438	0.001683435555281218
84-85	0.0	0.0	0.0	1.807560870223953	0.001683435555281218
86-87	0.0	0.0	0.0	1.9812914195289748	0.001683435555281218
88-89	0.0	0.0	0.0	2.1798245860151395	0.001683435555281218
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	795	0.0	65.78945	1
AAACCGT	520	0.0	65.73201	6
CCGTGCG	530	0.0	64.55337	9
ACCGTGC	555	0.0	61.593666	8
AACCGTG	575	0.0	60.270226	7
GTATCAA	1640	0.0	51.316864	1
TATCACG	65	4.4376065E-8	51.124897	2
ATCACGC	75	1.3684075E-7	44.308247	3
CGTGCGC	515	0.0	34.145226	10-11
CAAAACC	1015	0.0	34.14323	4
ATACAAA	1045	0.0	32.760227	1
ATCAACG	2535	0.0	32.585102	3
GTCTAAT	1125	0.0	32.54385	1
GCCCATA	1215	0.0	32.089893	1
TGCGCGG	510	0.0	31.687868	12-13
TATGTCG	60	0.0039614406	31.648746	5
GTGCGCG	520	0.0	31.53198	10-11
CCCATAA	1250	0.0	31.522152	2
CCATAAT	1260	0.0	31.271976	3
TCAACGC	2655	0.0	31.112328	4
>>END_MODULE