##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3224498_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891035 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.13976218667056 34.0 33.0 34.0 31.0 34.0 2 33.26254860920166 34.0 33.0 34.0 31.0 34.0 3 33.37419966667976 34.0 34.0 34.0 31.0 34.0 4 36.61266055766608 37.0 37.0 37.0 35.0 37.0 5 36.64517892114227 37.0 37.0 37.0 35.0 37.0 6 36.678519923459795 37.0 37.0 37.0 36.0 37.0 7 36.650103531286646 37.0 37.0 37.0 35.0 37.0 8 36.64209935636647 37.0 37.0 37.0 35.0 37.0 9 38.47297244215996 39.0 39.0 39.0 37.0 39.0 10-11 38.49112268317182 39.0 39.0 39.0 37.0 39.0 12-13 38.46457153759392 39.0 39.0 39.0 37.0 39.0 14-15 40.03311822767905 41.0 40.0 41.0 38.0 41.0 16-17 40.04005117644088 41.0 40.0 41.0 38.0 41.0 18-19 40.04038281324527 41.0 40.0 41.0 38.0 41.0 20-21 40.037591115949425 41.0 40.0 41.0 38.0 41.0 22-23 39.95506181014214 41.0 40.0 41.0 38.0 41.0 24-25 39.89031182837935 41.0 40.0 41.0 38.0 41.0 26-27 39.81150459858479 41.0 40.0 41.0 37.0 41.0 28-29 39.78007261218695 41.0 40.0 41.0 37.0 41.0 30-31 39.70075193454802 41.0 40.0 41.0 37.0 41.0 32-33 39.63434489105366 41.0 40.0 41.0 37.0 41.0 34-35 39.534915575706904 41.0 40.0 41.0 36.0 41.0 36-37 39.3878180991768 41.0 39.5 41.0 36.0 41.0 38-39 39.24086259237853 41.0 39.0 41.0 35.0 41.0 40-41 39.07381472108278 41.0 39.0 41.0 35.0 41.0 42-43 38.94026946191788 41.0 39.0 41.0 35.0 41.0 44-45 38.630708109109065 40.0 38.0 41.0 35.0 41.0 46-47 38.338712845174435 40.0 37.5 41.0 35.0 41.0 48-49 38.182632556521355 40.0 37.0 41.0 35.0 41.0 50-51 37.94873433703502 40.0 36.5 41.0 34.0 41.0 52-53 37.62040099434927 39.5 36.0 41.0 34.0 41.0 54-55 37.37498190306778 39.0 35.0 41.0 34.0 41.0 56-57 37.08238789722065 38.5 35.0 41.0 33.0 41.0 58-59 36.76229777730392 37.5 35.0 41.0 33.0 41.0 60-61 36.475086276072204 37.0 35.0 40.5 33.0 41.0 62-63 36.251187102639065 36.5 35.0 40.0 33.0 41.0 64-65 35.970296901917436 36.0 35.0 40.0 32.5 41.0 66-67 35.702888214267674 35.0 35.0 39.0 32.0 41.0 68-69 35.44776523930037 35.0 35.0 39.0 32.0 41.0 70-71 35.188649716341104 35.0 35.0 39.0 31.5 41.0 72-73 34.92073599802477 35.0 35.0 37.5 31.0 40.5 74-75 34.64450161890386 35.0 35.0 37.0 31.0 39.5 76-77 32.90325744779947 34.5 32.0 35.5 28.0 38.0 78-79 34.25338510832908 35.0 34.5 36.0 31.0 39.0 80-81 34.24236141116791 35.0 35.0 36.0 31.0 38.0 82-83 34.07622427850758 35.0 35.0 36.0 31.0 37.0 84-85 33.91903741154949 35.0 35.0 35.0 31.0 37.0 86-87 33.74967930552672 35.0 34.5 35.0 31.0 36.5 88-89 33.635091775295024 35.0 34.0 35.0 31.0 36.0 90-91 33.50235007603517 35.0 34.0 35.0 31.0 36.0 92-93 33.413898443944404 35.0 34.0 35.0 31.0 36.0 94-95 33.32633566582682 35.0 34.0 35.0 30.0 35.5 96-97 33.29443792892535 35.0 34.0 35.0 30.0 35.0 98-99 33.2823559119451 35.0 34.0 35.0 30.0 35.0 100-101 32.40651601788931 34.5 32.5 35.0 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 2.0 13 2.0 14 23.0 15 85.0 16 164.0 17 449.0 18 812.0 19 1202.0 20 1646.0 21 2359.0 22 3549.0 23 3221.0 24 2690.0 25 3205.0 26 4001.0 27 5456.0 28 6850.0 29 7552.0 30 8083.0 31 8696.0 32 10287.0 33 13814.0 34 23865.0 35 50691.0 36 147892.0 37 279835.0 38 232948.0 39 71642.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81503498947948 19.94851370825337 13.689422419878264 23.547028882388894 2 16.029785586424776 20.162956561751223 38.20512101095916 25.602136840864837 3 18.034757332764705 28.548485749718022 29.245764756715502 24.170992160801763 4 12.708816152003008 17.57832183920946 39.574651949698946 30.13821005908859 5 13.21586694125371 39.28936573759729 34.350165818402196 13.144601502746806 6 27.92864477826348 37.99300813099373 20.090344374800093 13.988002715942697 7 25.40394036148973 31.848805041328344 23.89513318780968 18.852121409372245 8 26.86426459117768 32.878169768864296 20.72522403721515 19.53234160274288 9 27.21430695763915 14.409422749948094 21.293102964530014 37.08316732788274 10-11 26.89232185043236 24.548137839703266 27.090686673363003 21.468853636501372 12-13 26.938335755610048 22.923454185301363 27.77343202006655 22.36477803902204 14-15 24.202977436352107 24.79251656781159 24.81501848973385 26.189487506102456 16-17 23.424388492034545 26.91241084805872 26.198746401656503 23.464454258250235 18-19 23.284102195761108 26.26406370120141 27.24500159926378 23.2068325037737 20-21 25.306275448134063 24.381332573162684 26.453040757417273 23.859351221285976 22-23 25.592211217418143 25.607867343789454 25.442832692685393 23.35708874610701 24-25 24.54145918276876 25.17908988780438 25.907177399714715 24.372273529712142 26-27 23.859744617810534 26.49656151534622 26.40840387534476 23.235289991498483 28-29 23.831986267277433 26.356198944905813 26.71966267513121 23.09215211268554 30-31 23.956598445840086 25.020201385366114 26.87524971156911 24.14795045722469 32-33 24.148378009842485 25.245248503145216 27.197192029493788 23.409181457518503 34-35 24.661587723443013 25.144023730666365 26.29784096278447 23.896547583106155 36-37 23.6895193534141 25.272202541249143 27.08939264448493 23.948885460851823 38-39 23.880655642034263 25.470155493330786 26.075294460935876 24.57389440369907 40-41 24.21341473679485 25.357870341793532 26.67661764128233 23.752097280129288 42-43 24.366046227140348 25.41364817319185 26.824198824961982 23.396106774705817 44-45 24.39545342816769 25.12332244990499 26.277811387081528 24.203412734845788 46-47 24.220042523224354 24.865472287459692 25.883626087649958 25.030859101665996 48-49 24.03640956893122 24.878918150296933 26.337511833222134 24.747160447549714 50-51 24.32121072685136 25.369486047124973 25.79994051861038 24.509362707413292 52-53 24.91671197916579 25.459620250394345 25.161539760806118 24.462128009633748 54-55 24.174302917393817 24.852839675209168 25.8988143002239 25.074043107173118 56-57 24.662920150264714 25.496233841885335 25.177308546614274 24.663537461235677 58-59 25.302197015669392 25.124300328230337 25.50569196422306 24.067810691877213 60-61 24.505669498094637 25.637436144123853 25.571370115141246 24.28552424264026 62-63 24.320256780036697 25.302036395876705 25.990337079912685 24.38736974417391 64-65 25.02909537784711 25.006312883332306 25.335087847278725 24.62950389154186 66-67 24.224469296941198 25.084985438282448 25.3947936949727 25.295751569803652 68-69 24.31268132003793 25.457585841184688 26.18488611558469 24.04484672319269 70-71 25.178865027748632 24.949861677711873 25.76385888320885 24.107414411330645 72-73 24.78724180307171 25.034931287772082 25.941180761698472 24.236646147457733 74-75 24.591177675399955 24.756434932410063 25.904313523037814 24.748073869152165 76-77 24.669218385167333 24.16261565923167 25.41991967754299 25.748246278058005 78-79 24.471710745253322 24.130956810336652 25.747517720944302 25.649814723465724 80-81 23.86669734449707 25.324642528451818 25.89985184852634 24.90880827852477 82-83 24.07599549508348 25.407605722164266 26.01998930447074 24.49640947828151 84-85 24.02772781934382 24.70560375570204 26.462020710007707 24.804647714946434 86-87 24.082275107038445 25.75084031491468 25.721604650771297 24.445279927275585 88-89 24.466266757198092 24.972083027041585 25.05625480480565 25.50539541095468 90-91 24.335632158108268 24.911142659940406 25.41106690534042 25.34215827661091 92-93 24.304095798705998 24.988356237409302 25.961887019028435 24.74566094485626 94-95 24.484335632158107 24.713114524120826 25.687262565443557 25.11528727827751 96-97 24.436245489795574 25.68883377196182 25.787034179353224 24.08788655888938 98-99 22.718243391112583 26.411476541325534 26.12697593248301 24.74330413507887 100-101 24.069763850712995 25.277884623483533 25.67825186075306 24.97409966505041 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 1.5 3 4.5 4 4.5 5 6.0 6 6.5 7 11.0 8 25.0 9 39.5 10 40.0 11 37.0 12 52.5 13 75.0 14 84.5 15 93.0 16 108.5 17 112.5 18 114.5 19 138.0 20 176.0 21 225.5 22 283.5 23 361.0 24 546.5 25 825.5 26 1316.0 27 1921.5 28 2637.0 29 3780.5 30 5555.5 31 7341.5 32 8872.5 33 10803.0 34 12555.5 35 14141.5 36 16185.5 37 17494.0 38 18219.5 39 19161.5 40 19827.0 41 19934.5 42 19981.0 43 21413.0 44 23701.5 45 26686.5 46 29678.5 47 31787.0 48 34818.0 49 40566.5 50 46485.0 51 45135.5 52 41209.5 53 43357.5 54 46876.5 55 46678.0 56 39393.0 57 32023.0 58 29750.0 59 28627.5 60 23534.5 61 16598.5 62 13092.5 63 8968.0 64 5016.0 65 3219.5 66 2778.5 67 2280.5 68 1605.5 69 1004.5 70 496.5 71 289.5 72 213.5 73 193.5 74 152.0 75 104.5 76 68.5 77 45.0 78 32.0 79 15.5 80 11.0 81 10.5 82 5.5 83 1.5 84 3.5 85 3.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47102526836768477 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.85603259131235E-4 22-23 0.0011222903701874785 24-25 2.244580740374957E-4 26-27 0.0019640081478280876 28-29 0.002749611406959323 30-31 7.85603259131235E-4 32-33 0.0 34-35 0.004040245332674923 36-37 0.0018517791108093398 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.008753864887462333 46-47 0.078335867839086 48-49 6.172597036031133E-4 50-51 0.0 52-53 5.6114518509373924E-5 54-55 0.0 56-57 0.00830494873938734 58-59 0.03922404843805238 60-61 0.029123435106365073 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.683435555281218E-4 78-79 0.007799918072802976 80-81 0.006172597036031132 82-83 9.539468146593568E-4 84-85 0.0018517791108093398 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.06761799480379559 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 891035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.164618716853326 #Duplication Level Percentage of deduplicated Percentage of total 1 70.40465039296485 28.277759389267903 2 15.833920288967368 12.719267423988445 3 5.844419845467014 7.042166841431803 4 2.7443791425782136 4.409077675045552 5 1.4697414492613416 2.9515802460968605 6 0.8370986803340354 2.017304959439857 7 0.5536121341520432 1.5564934199668188 8 0.3915421986522241 1.2580914496340019 9 0.28125470589161744 1.016683922201178 >10 1.3034196189379672 9.57480967248956 >50 0.14278384020167173 4.088052375548642 >100 0.165180934100406 14.613511098869672 >500 0.01987770562879167 5.238387898682088 >1k 0.008119062862464204 5.236813627337631 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC 3435 0.3855067421593989 No Hit ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA 2457 0.2757467439550635 No Hit GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT 2445 0.2743999955108385 No Hit GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA 2416 0.27114535343729484 No Hit ATACAAAACCGTGCGCGGCTTAACCCGCGGTCTAATGTTATTAAATATGT 2389 0.2681151694377886 No Hit TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA 2018 0.22647819670383318 No Hit TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG 1916 0.21503083492792088 No Hit CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG 1828 0.20515467967027107 No Hit TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG 1742 0.1955029824866588 No Hit GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG 1637 0.18371893359969024 No Hit ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA 1623 0.1821477270814278 No Hit GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC 1546 0.17350609123098418 No Hit GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT 1523 0.17092482337955298 No Hit GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC 1502 0.16856801360215928 No Hit ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG 1464 0.16430331019544686 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG 1445 0.16217095849209068 No Hit ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT 1417 0.15902854545556572 No Hit GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT 1402 0.1573451099002845 No Hit CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA 1361 0.15274371938251582 No Hit GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG 1275 0.14309202219890352 No Hit TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC 1169 0.13119574427491626 No Hit GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA 1129 0.12670658279416633 No Hit ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG 1102 0.12367639879466014 No Hit CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA 1080 0.12120735998024769 No Hit TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT 1056 0.11851386309179775 No Hit GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC 1051 0.11795271790670399 No Hit CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA 1051 0.11795271790670399 No Hit GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC 1051 0.11795271790670399 No Hit GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC 1041 0.11683042753651651 No Hit GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT 959 0.10762764650097918 No Hit GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA 935 0.10493414961252924 No Hit GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA 930 0.10437300442743551 No Hit ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA 909 0.1020161946500418 No Hit GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC 905 0.10156727850196683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1222903701874786E-4 2 0.0 0.0 0.0 0.0 1.1222903701874786E-4 3 0.0 0.0 0.0 0.0 1.1222903701874786E-4 4 0.0 0.0 0.0 0.0 1.1222903701874786E-4 5 0.0 0.0 0.0 0.0 1.1222903701874786E-4 6 0.0 0.0 0.0 0.0 1.1222903701874786E-4 7 0.0 0.0 0.0 0.0 1.1222903701874786E-4 8 0.0 0.0 0.0 0.0 1.1222903701874786E-4 9 0.0 0.0 0.0 0.0 1.1222903701874786E-4 10-11 0.0 0.0 0.0 0.0 1.683435555281218E-4 12-13 0.0 0.0 0.0 5.611451850937393E-5 6.172597036031133E-4 14-15 0.0 0.0 0.0 1.683435555281218E-4 6.733742221124872E-4 16-17 0.0 0.0 0.0 2.2445807403749572E-4 6.733742221124872E-4 18-19 0.0 0.0 0.0 2.2445807403749572E-4 6.733742221124872E-4 20-21 0.0 0.0 0.0 2.2445807403749572E-4 7.85603259131235E-4 22-23 0.0 0.0 0.0 3.366871110562436E-4 7.85603259131235E-4 24-25 0.0 0.0 0.0 3.366871110562436E-4 7.85603259131235E-4 26-27 0.0 0.0 0.0 6.172597036031133E-4 7.85603259131235E-4 28-29 0.0 0.0 0.0 0.0011784048886968525 7.85603259131235E-4 30-31 0.0 0.0 0.0 0.0028057259254686967 7.85603259131235E-4 32-33 0.0 0.0 0.0 0.0068459712581436195 7.85603259131235E-4 34-35 0.0 0.0 0.0 0.011447361775912282 8.978322961499829E-4 36-37 0.0 0.0 0.0 0.020369570218902736 8.978322961499829E-4 38-39 0.0 0.0 0.0 0.045452759992592885 8.978322961499829E-4 40-41 0.0 0.0 0.0 0.07485676769150482 8.978322961499829E-4 42-43 0.0 0.0 0.0 0.10128670590941995 8.978322961499829E-4 44-45 0.0 0.0 0.0 0.12597709405354449 8.978322961499829E-4 46-47 0.0 0.0 0.0 0.16031917938128132 8.978322961499829E-4 48-49 0.0 0.0 0.0 0.19993602944889932 9.539468146593568E-4 50-51 0.0 0.0 0.0 0.23775721492421736 0.0010100613331687307 52-53 0.0 0.0 0.0 0.2780474392139478 0.0011222903701874785 54-55 0.0 0.0 0.0 0.3189549232072814 0.0011222903701874785 56-57 0.0 0.0 0.0 0.36732563816236175 0.0011222903701874785 58-59 0.0 0.0 0.0 0.42428187444937626 0.0011222903701874785 60-61 0.0 0.0 0.0 0.4939199919195093 0.0011222903701874785 62-63 0.0 0.0 0.0 0.5656904610929986 0.0011222903701874785 64-65 0.0 0.0 0.0 0.6293243250826286 0.0011222903701874785 66-67 0.0 0.0 0.0 0.7031710314409647 0.0011222903701874785 68-69 0.0 0.0 0.0 0.7948621546852818 0.0011222903701874785 70-71 0.0 0.0 0.0 0.8914352410399142 0.0011222903701874785 72-73 0.0 0.0 0.0 1.0055721716879809 0.0011222903701874785 74-75 0.0 0.0 0.0 1.119316300706482 0.0011222903701874785 76-77 0.0 0.0 0.0 1.2274489778740454 0.0011222903701874785 78-79 0.0 0.0 0.0 1.3546605913347962 0.0012345194072062265 80-81 0.0 0.0 0.0 1.50577698968054 0.0012345194072062265 82-83 0.0 0.0 0.0 1.669631383727912 0.0013467484442249743 84-85 0.0 0.0 0.0 1.842688558810821 0.0013467484442249743 86-87 0.0 0.0 0.0 2.0199543227819334 0.0015712065182624702 88-89 0.0 0.0 0.0 2.2219104748971703 0.0017956645922999658 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAT 25 5.1575833E-5 76.34499 1 GGTATCA 800 0.0 70.97698 1 AAACCGT 510 0.0 57.730534 6 CCGTGCG 510 0.0 56.799397 9 GTATCAA 1950 0.0 54.322395 1 ACCGTGC 555 0.0 53.04968 8 AACCGTG 570 0.0 51.653637 7 ATCAACG 2895 0.0 36.907784 3 TCAACGC 3050 0.0 35.032143 4 TATCAAC 3250 0.0 32.87632 2 GTCTAAT 1265 0.0 31.684679 1 TATCACG 105 1.9047857E-6 31.658678 2 CAAAACC 960 0.0 31.658678 4 ATCACGC 105 1.9047857E-6 31.658678 3 CGCAGAG 3495 0.0 30.571686 8 TCTAATG 1305 0.0 30.567003 2 CTAATGT 1300 0.0 30.319273 3 ACGCAGA 3610 0.0 29.597797 7 GTACATG 5270 0.0 29.516684 1 ATACAAA 970 0.0 29.514814 1 >>END_MODULE