FastQCFastQC Report
Fri 10 Feb 2017
SRR3224497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225393
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG26030.2124216475857133No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA25990.2120952216962232No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24110.19675320489018622No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA20870.17031270784148433No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC16210.13228409171588218No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA15740.12844858751437294No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA15370.12542914803658908No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC14450.1179213525783157No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14280.11653404254798255No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC14200.11588119076900227No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC13790.11253532540172827No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT13510.11025034417529722No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA13080.10674126586327816No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA12990.10600680761192532No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA12970.10584359466718025No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG12590.10274254871702385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19200.049.2782941
GGTATCA9300.048.5673521
CCGTGCG2150.046.405069
AAACCGT2500.041.7831576
ATCAACG25100.036.8875243
TCAACGC25800.035.88674
AACCGTG2650.035.8346147
ACCGTGC3200.035.61218
AATGCGG2950.030.5808947
ACGCAGA30500.030.200947
CGCAGAG30700.030.0054198
GTATAGA3500.029.8853551
GTACATG54050.029.5561181
TATCAAC32750.028.2710482
TACCGTA1554.605863E-827.5695347
ACATGGG55050.027.4276373
ATCGCGT350.00837147627.1574112-13
TGTACCG2104.3655746E-1127.1319225
TACATGG57550.026.9787032
AAATGCG3400.026.5334226
Produced by FastQC (version 0.11.5)