##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224497_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225393
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29042029781466	34.0	31.0	34.0	31.0	34.0
2	32.45678243632859	34.0	31.0	34.0	31.0	34.0
3	32.599375873699294	34.0	31.0	34.0	31.0	34.0
4	35.88170978616656	37.0	37.0	37.0	35.0	37.0
5	35.89933188781069	37.0	37.0	37.0	35.0	37.0
6	35.99440342812469	37.0	37.0	37.0	35.0	37.0
7	35.953534906760524	37.0	37.0	37.0	35.0	37.0
8	35.988384950787214	37.0	37.0	37.0	35.0	37.0
9	37.72625435268522	39.0	39.0	39.0	35.0	39.0
10-11	37.8039702364874	39.0	39.0	39.0	36.0	39.0
12-13	37.800164926680665	39.0	39.0	39.0	35.0	39.0
14-15	39.3053032782136	41.0	40.0	41.0	37.0	41.0
16-17	39.14591930915225	41.0	40.0	41.0	36.5	41.0
18-19	39.226418381694685	41.0	40.0	41.0	36.5	41.0
20-21	39.291696215010205	41.0	40.0	41.0	37.0	41.0
22-23	39.25008058639147	41.0	40.0	41.0	36.5	41.0
24-25	39.18918175638346	41.0	40.0	41.0	36.0	41.0
26-27	39.028848704048414	41.0	39.0	41.0	35.5	41.0
28-29	38.961327916839736	41.0	39.0	41.0	35.0	41.0
30-31	38.890313148516434	41.0	39.0	41.0	35.0	41.0
32-33	38.70211760635159	41.0	39.0	41.0	35.0	41.0
34-35	38.7053528949488	41.0	39.0	41.0	35.0	41.0
36-37	38.695849821241026	41.0	39.0	41.0	35.0	41.0
38-39	38.585748408877805	41.0	39.0	41.0	35.0	41.0
40-41	38.494144735607264	40.0	39.0	41.0	35.0	41.0
42-43	38.345642989636794	40.0	38.0	41.0	34.0	41.0
44-45	38.17079010570487	40.0	38.0	41.0	34.0	41.0
46-47	38.00714423862385	40.0	38.0	41.0	33.0	41.0
48-49	37.791385702382826	40.0	37.0	41.0	33.0	41.0
50-51	36.495487162077794	38.5	35.5	40.0	31.0	40.5
52-53	36.80614790520266	39.0	35.5	40.0	31.5	40.5
54-55	37.25036743314186	40.0	36.0	41.0	33.0	41.0
56-57	37.088399395132825	39.0	35.0	41.0	32.0	41.0
58-59	36.934595268619944	39.0	35.0	41.0	32.0	41.0
60-61	36.68822655262434	39.0	35.0	41.0	32.0	41.0
62-63	36.41373502215208	38.0	35.0	41.0	31.5	41.0
64-65	36.17391400146728	37.5	35.0	41.0	31.0	41.0
66-67	35.950503634344244	37.0	35.0	40.0	31.0	41.0
68-69	35.6724352921879	36.5	35.0	40.0	31.0	41.0
70-71	35.39992679899429	36.0	35.0	39.5	30.0	41.0
72-73	35.10378507140158	35.5	35.0	39.0	30.0	41.0
74-75	34.83952454437066	35.0	35.0	39.0	30.0	41.0
76-77	34.522601320555935	35.0	34.0	37.5	29.0	40.0
78-79	34.28422391836742	35.0	34.0	37.0	29.0	39.0
80-81	33.9734717759935	35.0	34.0	37.0	29.0	39.0
82-83	33.708137715818516	35.0	34.0	36.0	29.0	38.5
84-85	33.4710847866766	35.0	34.0	36.0	29.0	37.0
86-87	33.2336368822084	35.0	34.0	35.5	29.0	37.0
88-89	33.08452186359804	35.0	34.0	35.0	29.0	36.5
90-91	32.90342771665907	35.0	34.0	35.0	27.5	36.0
92-93	32.76200737232871	35.0	34.0	35.0	27.0	36.0
94-95	32.63350614863966	35.0	33.0	35.0	27.0	36.0
96-97	32.50206872407465	35.0	33.0	35.0	27.0	35.5
98-99	32.46285273377602	35.0	33.0	35.0	27.0	35.0
100-101	31.286246126752808	34.5	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	274.0
8	841.0
9	590.0
10	623.0
11	1134.0
12	1782.0
13	2160.0
14	2172.0
15	2273.0
16	2446.0
17	2651.0
18	2987.0
19	3433.0
20	4110.0
21	5276.0
22	6386.0
23	6141.0
24	5970.0
25	6471.0
26	6709.0
27	6949.0
28	7485.0
29	8821.0
30	10727.0
31	13589.0
32	18141.0
33	25824.0
34	42589.0
35	77926.0
36	170235.0
37	295642.0
38	349509.0
39	133503.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93746644033388	20.469986523010185	13.971200596289373	23.621346440366565
2	15.40917893279952	20.73865282403278	39.139198608120005	24.712969635047696
3	18.714893915666238	27.76978487717818	29.17749652560444	24.337824681551144
4	12.580453780950274	17.41367871368614	40.11366149472047	29.892206010643118
5	13.275659319091915	39.45077211963836	34.318867498019	12.95470106325073
6	28.35751469120519	38.513276965022655	19.785734046138668	13.343474297633495
7	25.990110927677897	32.02107405542549	23.73426321188386	18.25455180501276
8	25.382387527919615	34.85942877101469	21.448547527201477	18.30963617386422
9	26.421645953583873	15.888127319153936	21.09453865004941	36.59568807721278
10-11	25.322080344836312	25.61557802272414	28.30136943821288	20.760972194226667
12-13	25.694450678272197	23.36532035028762	28.512975021074872	22.427253950365312
14-15	23.132489400496922	25.18361110895681	25.57016081202378	26.11373867852249
16-17	22.997182100190813	27.8135816972487	26.55832799439115	22.630908208169338
18-19	22.94728765378941	27.31614143421823	27.1959872417797	22.54058367021266
20-21	24.128279450200605	25.72744576276237	26.81159863974735	23.33267614728968
22-23	24.37241111802954	26.46291098022804	26.08956077046352	23.0751171312789
24-25	23.47428914700906	26.45445619144242	26.48681870381812	23.584435957730395
26-27	23.250989682493696	27.302506216373036	26.68294987812073	22.763554223012537
28-29	23.07427086657097	27.146352231488187	26.94706922595445	22.8323076759864
30-31	23.278123834557565	26.398877747791932	26.935970745711785	23.387027671938718
32-33	23.162446659969497	26.402713251993443	27.266966597654797	23.167873490382267
34-35	23.7983242926963	26.216936117637363	26.62782470603309	23.35691488363325
36-37	23.195462071493026	26.24262278489692	27.196839872651342	23.36507527095872
38-39	23.05723912318737	26.633638681018784	26.479353020363178	23.82976917543067
40-41	23.68447898202283	26.093662778740782	27.05707514371615	23.16478309552023
42-43	23.515703835668646	26.42874076746017	27.015673436277776	23.039881960593412
44-45	23.47248996541148	26.122535425076432	26.597658296756123	23.807316312755965
46-47	23.747524263644397	25.883288055342245	26.43368290825882	23.935504772754538
48-49	23.13045692279946	26.29625760878347	26.81303059508256	23.760254873334514
50-51	23.228629509063623	26.58236990092158	26.406140723833087	23.782859866181706
52-53	23.50074629118985	26.49390848486975	25.943758451370297	24.061586772570106
54-55	23.582556779743317	25.957305125784135	26.34456864042801	24.11556945404454
56-57	23.468979722407195	26.35173071147845	26.31223314661757	23.867056419496784
58-59	23.80372689868846	26.190641372313607	26.60469588255315	23.40093584644479
60-61	23.540898587891338	26.596672023663288	26.31328717446859	23.549142213976783
62-63	23.189537372474174	26.33913338764422	26.816666476197625	23.654662763683977
64-65	24.09013327389547	25.813545546099654	26.073953601726313	24.022367578278566
66-67	23.29120216065946	26.365073256493016	25.976232909164885	24.367491673682633
68-69	23.373276981343942	26.358278527786595	27.074089700202304	23.194354790667155
70-71	23.819256352859856	25.92772277954909	26.568864029743928	23.684156837847123
72-73	23.499685407049004	26.155241624523722	26.87178725519078	23.47328571323649
74-75	23.604100888449665	25.903893689616307	26.72110090395489	23.77090451797913
76-77	23.267882222274814	25.627002928856296	26.35399418798704	24.751120660881856
78-79	23.60089896371048	25.180941544913292	26.822892967082208	24.39526652429402
80-81	23.17793760954298	26.35882290260274	26.35821050298014	24.10502898487414
82-83	23.072008608766506	26.53147702723225	26.65782439226713	23.738689971734114
84-85	23.377183655860613	25.621031502275233	27.084046746285935	23.91773809557822
86-87	23.318013816552018	26.73859232189684	26.514004966969207	23.429388894581937
88-89	23.57732868943093	25.936600364660357	26.221871376203033	24.26419956970568
90-91	23.213736327855635	25.842852048281657	26.53022336507553	24.41318825878718
92-93	23.338307404102242	25.76238141696954	26.942284865318566	23.957026313609653
94-95	23.62446170330661	25.505939727091636	26.816743689575507	24.052854880026246
96-97	23.648494809420324	26.259738712396757	26.68433718815107	23.40742929003185
98-99	22.809679076502864	26.335957133489828	26.86546397031791	23.988899819689397
100-101	23.523149178149577	25.774854010054476	26.441111095235858	24.26088571656009
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	5.5
3	8.0
4	5.5
5	5.0
6	7.0
7	13.5
8	22.0
9	32.0
10	40.5
11	46.5
12	56.5
13	60.5
14	66.0
15	76.0
16	85.5
17	89.5
18	100.5
19	112.5
20	119.5
21	154.5
22	198.0
23	249.0
24	315.5
25	401.5
26	577.5
27	826.5
28	1131.0
29	1764.5
30	3032.0
31	5743.0
32	10162.0
33	16417.5
34	24548.5
35	33890.5
36	45041.0
37	53102.5
38	53268.0
39	49391.5
40	44606.5
41	39636.5
42	35529.0
43	32685.5
44	31295.0
45	32689.0
46	36036.0
47	37124.0
48	39468.0
49	44771.0
50	48993.0
51	48979.5
52	47603.0
53	49128.5
54	52017.5
55	52149.0
56	44115.0
57	36361.0
58	33859.0
59	32319.5
60	27471.5
61	20830.5
62	16824.5
63	12303.5
64	7570.5
65	4883.5
66	3889.0
67	3327.0
68	2525.0
69	1641.0
70	1020.5
71	678.5
72	505.0
73	380.0
74	279.0
75	198.0
76	138.0
77	91.0
78	60.5
79	42.0
80	30.5
81	21.5
82	19.5
83	18.5
84	13.0
85	13.5
86	13.0
87	12.5
88	10.0
89	6.0
90	3.5
91	4.5
92	5.5
93	5.0
94	3.5
95	3.5
96	5.0
97	5.0
98	4.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14901341855225222
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.037008535220945445
16-17	0.07340502189909685
18-19	0.02452274494794731
20-21	0.038640664668396184
22-23	0.06573401349607841
24-25	0.01713735919823273
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.020605634274065546
38-39	0.005590043357518771
40-41	0.0049779948147247455
42-43	0.0032234556587152044
44-45	0.010690447880802322
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	8.160647237253682E-5
58-59	0.015668442695527068
60-61	0.01664772036399751
62-63	0.028317445913270275
64-65	0.04769898310174776
66-67	0.017259768906791536
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016688523600183778
80-81	0.05732854684170711
82-83	0.048555851061659404
84-85	0.028929494456064297
86-87	0.05732854684170711
88-89	0.03407070221553412
90-91	0.0
92-93	0.0031418491863426674
94-95	0.0
96-97	0.0
98-99	6.120485427940261E-4
100-101	0.046148460126669566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1225393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.987439810196854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.092140857316	29.010021056041985
2	15.68807741786149	13.174044126403356
3	6.099830741317502	7.683488283103712
4	2.9436137983597654	4.9437922873238245
5	1.6595229755056635	3.483956052384142
6	1.011017111310644	2.5470012064940857
7	0.6907049520689409	2.0300652821119693
8	0.4966705326551861	1.6683139276286432
9	0.345121624720061	1.304169610061782
>10	1.699268335272492	12.62163620290369
>50	0.12925204584771355	3.7861946308724304
>100	0.1258696198861922	10.975478128122836
>500	0.014773428029994049	4.100420676887768
>1k	0.004136559848398333	2.6714185296598267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2603	0.2124216475857133	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2599	0.2120952216962232	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2411	0.19675320489018622	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	2087	0.17031270784148433	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1621	0.13228409171588218	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1574	0.12844858751437294	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1537	0.12542914803658908	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1445	0.1179213525783157	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1428	0.11653404254798255	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1420	0.11588119076900227	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1379	0.11253532540172827	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1351	0.11025034417529722	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1308	0.10674126586327816	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1299	0.10600680761192532	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1297	0.10584359466718025	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1259	0.10274254871702385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	8.160647237253682E-5	0.0
18-19	0.0	0.0	0.0	1.2240970855880522E-4	0.0
20-21	0.0	0.0	0.0	2.8562265330387885E-4	0.0
22-23	0.0	0.0	0.0	5.712453066077577E-4	0.0
24-25	0.0	0.0	0.0	6.528517789802944E-4	0.0
26-27	0.0	0.0	0.0	8.976711960979049E-4	0.0
28-29	0.0	0.0	0.0	0.0016321294474507361	0.0
30-31	0.0	0.0	0.0	0.004488355980489524	0.0
32-33	0.0	0.0	0.0	0.012240970855880523	0.0
34-35	0.0	0.0	0.0	0.02150330547016345	0.0
36-37	0.0	0.0	0.0	0.03737576434662186	0.0
38-39	0.0	0.0	0.0	0.06928389504428376	0.0
40-41	0.0	0.0	0.0	0.10902624708970918	0.0
42-43	0.0	0.0	0.0	0.1462796017277722	0.0
44-45	0.0	0.0	0.0	0.18769488645683466	0.0
46-47	0.0	0.0	0.0	0.2386581284534839	0.0
48-49	0.0	0.0	0.0	0.2992509341900925	0.0
50-51	0.0	0.0	0.0	0.363638440892024	0.0
52-53	0.0	0.0	0.0	0.42590417931226965	0.0
54-55	0.0	0.0	0.0	0.49237265105970085	0.0
56-57	0.0	0.0	0.0	0.5722653875124144	0.0
58-59	0.0	0.0	0.0	0.6599515420767053	0.0
60-61	0.0	0.0	0.0	0.7607355354567882	0.0
62-63	0.0	0.0	0.0	0.8655590492193117	0.0
64-65	0.0	0.0	0.0	0.9785024069829027	0.0
66-67	0.0	0.0	0.0	1.105522881230756	0.0
68-69	0.0	0.0	0.0	1.252496137973695	0.0
70-71	0.0	0.0	0.0	1.412567233532426	0.0
72-73	0.0	0.0	0.0	1.5812478119264595	0.0
74-75	0.0	0.0	0.0	1.750540438863287	0.0
76-77	0.0	0.0	0.0	1.9295034327762604	0.0
78-79	0.0	0.0	0.0	2.1413130318191795	0.0
80-81	0.0	0.0	0.0	2.390049559610672	0.0
82-83	0.0	0.0	0.0	2.65425051391676	0.0
84-85	0.0	0.0	0.0	2.9234294630375723	0.0
86-87	0.0	0.0	0.0	3.197055964902688	0.0
88-89	0.0	0.0	0.0	3.5050387916366423	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1920	0.0	49.278294	1
GGTATCA	930	0.0	48.567352	1
CCGTGCG	215	0.0	46.40506	9
AAACCGT	250	0.0	41.783157	6
ATCAACG	2510	0.0	36.887524	3
TCAACGC	2580	0.0	35.8867	4
AACCGTG	265	0.0	35.834614	7
ACCGTGC	320	0.0	35.6121	8
AATGCGG	295	0.0	30.580894	7
ACGCAGA	3050	0.0	30.20094	7
CGCAGAG	3070	0.0	30.005419	8
GTATAGA	350	0.0	29.885355	1
GTACATG	5405	0.0	29.556118	1
TATCAAC	3275	0.0	28.271048	2
TACCGTA	155	4.605863E-8	27.569534	7
ACATGGG	5505	0.0	27.427637	3
ATCGCGT	35	0.008371476	27.15741	12-13
TGTACCG	210	4.3655746E-11	27.131922	5
TACATGG	5755	0.0	26.978703	2
AAATGCG	340	0.0	26.533422	6
>>END_MODULE