FastQCFastQC Report
Fri 10 Feb 2017
SRR3224497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225393
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG22090.18026869747093383No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT20960.17104716609283716No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA20620.1682725460321709No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG17910.14615719201921343No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA17580.14346417843091971No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG17240.14068955837025346No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG15470.12624521276031445No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT15190.1239602315338834No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG14870.12134882441796224No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG14610.11922705613627628No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG14540.11865581082966853No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA14380.11735010727170793No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC13850.11302496423596348No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA13830.1128617512912184No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT13660.11147444126088529No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13190.10763893705937606No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC12730.10388503933023938No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC12540.10233451635516116No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC12350.10078399338008295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.053.8623051
CCGTGCG2300.051.6203469
GTATCAA21550.047.34951
AAACCGT2950.041.8562246
AACCGTG3350.036.8584677
ATCAACG29050.034.8210753
ACCGTGC3600.034.2988478
TCAACGC30250.033.5967374
AAATGCG2550.029.79816
CGCAGAG36400.028.0508358
TATCAAC36950.028.0188792
ACGCAGA36800.027.8749877
CAACGCA37250.027.410755
GTACATG59300.026.9363651
AACGCAG38500.026.6441426
AATGCGG2700.026.3837327
GCCCATA9500.025.5973781
ATAACGC1301.0036985E-525.5719264
TACATGG62300.025.3080562
GTCTAAT7050.025.0242731
Produced by FastQC (version 0.11.5)