##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225393
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.169186538522744	34.0	33.0	34.0	31.0	34.0
2	33.29904446981499	34.0	34.0	34.0	31.0	34.0
3	33.41366076026222	34.0	34.0	34.0	31.0	34.0
4	36.62879011060125	37.0	37.0	37.0	35.0	37.0
5	36.65925625493209	37.0	37.0	37.0	35.0	37.0
6	36.69998604529322	37.0	37.0	37.0	36.0	37.0
7	36.67661395160573	37.0	37.0	37.0	36.0	37.0
8	36.666440072695046	37.0	37.0	37.0	35.0	37.0
9	38.509798897170135	39.0	39.0	39.0	37.0	39.0
10-11	38.53623817012175	39.0	39.0	39.0	37.5	39.0
12-13	38.5245072397182	39.0	39.0	39.0	37.0	39.0
14-15	40.13200499758037	41.0	40.0	41.0	38.0	41.0
16-17	40.143655953640994	41.0	40.0	41.0	38.0	41.0
18-19	40.13374403150662	41.0	40.0	41.0	38.0	41.0
20-21	40.1351395021842	41.0	40.0	41.0	38.0	41.0
22-23	40.0642349025986	41.0	40.0	41.0	38.0	41.0
24-25	40.023569989383	41.0	40.0	41.0	38.0	41.0
26-27	39.96209583374477	41.0	40.0	41.0	38.0	41.0
28-29	39.93436881065911	41.0	40.0	41.0	38.0	41.0
30-31	39.880047462324335	41.0	40.0	41.0	38.0	41.0
32-33	39.83983505699804	41.0	40.0	41.0	38.0	41.0
34-35	39.76158587489891	41.0	40.0	41.0	37.0	41.0
36-37	39.65345444277877	41.0	40.0	41.0	37.0	41.0
38-39	39.54742274519277	41.0	40.0	41.0	37.0	41.0
40-41	39.418799519827516	41.0	39.0	41.0	36.0	41.0
42-43	39.32420904966815	41.0	39.0	41.0	35.5	41.0
44-45	39.09204883657733	41.0	39.0	41.0	35.0	41.0
46-47	38.87240052783066	40.5	38.5	41.0	35.0	41.0
48-49	38.768490598526355	40.0	38.0	41.0	35.0	41.0
50-51	38.60169390554704	40.0	38.0	41.0	35.0	41.0
52-53	38.347734971556065	40.0	37.0	41.0	35.0	41.0
54-55	38.15277996528461	40.0	36.5	41.0	35.0	41.0
56-57	37.920708295216315	40.0	36.0	41.0	34.5	41.0
58-59	37.65686028890323	39.0	35.0	41.0	34.0	41.0
60-61	37.41099753303634	39.0	35.0	41.0	34.0	41.0
62-63	37.20060666251562	39.0	35.0	41.0	34.0	41.0
64-65	36.940371374734475	38.0	35.0	41.0	33.0	41.0
66-67	36.67577258887557	37.0	35.0	40.0	33.0	41.0
68-69	36.42309732469502	37.0	35.0	40.0	33.0	41.0
70-71	36.15148731876222	36.0	35.0	39.5	33.0	41.0
72-73	35.86007305411407	36.0	35.0	39.0	33.0	41.0
74-75	35.556601841205236	35.0	35.0	39.0	32.5	41.0
76-77	33.69996890793403	34.5	32.5	36.5	29.5	39.0
78-79	35.011908424480964	35.0	35.0	37.0	32.5	39.0
80-81	34.92883670789698	35.0	35.0	37.0	33.0	39.0
82-83	34.708544524083294	35.0	35.0	36.0	33.0	38.5
84-85	34.48702457089277	35.0	35.0	36.0	33.0	37.0
86-87	34.27576907979726	35.0	35.0	36.0	32.0	37.0
88-89	34.123664408071534	35.0	35.0	35.0	32.0	36.5
90-91	33.97174865532935	35.0	35.0	35.0	32.0	36.0
92-93	33.86823043709242	35.0	35.0	35.0	32.0	36.0
94-95	33.76439150541908	35.0	34.0	35.0	32.0	36.0
96-97	33.70864081972069	35.0	34.0	35.0	31.0	36.0
98-99	33.678091028755674	35.0	34.0	35.0	31.0	35.0
100-101	32.850105231546124	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	12.0
15	65.0
16	146.0
17	304.0
18	517.0
19	811.0
20	1227.0
21	1829.0
22	2402.0
23	2662.0
24	2524.0
25	3122.0
26	4153.0
27	5705.0
28	7258.0
29	8299.0
30	9219.0
31	9789.0
32	12103.0
33	16343.0
34	27629.0
35	57093.0
36	161046.0
37	332827.0
38	400829.0
39	157451.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.383416375533855	20.063197888593635	13.871969342300853	23.681416393571656
2	15.366580354221055	20.702827582661236	39.26715755680015	24.663434506317568
3	18.59909433136961	27.832948286794522	29.104132306941526	24.46382507489434
4	12.68931681509524	17.54890063840743	39.9834175648139	29.778364981683424
5	13.363957522199001	38.6266283551481	34.71286354663361	13.296550576019287
6	28.477884237954683	38.004705429197	19.921037577332335	13.596372755515985
7	26.229952349980785	31.715212996973214	23.71908440802257	18.335750245023434
8	25.55955517944039	34.45955705638926	21.20519702658657	18.77569073758378
9	26.48505418261733	15.9613283248721	21.048349386686557	36.50526810582401
10-11	25.50136976463877	25.52993202996916	28.204094523144818	20.764603682247245
12-13	26.031485409170774	23.40285932757899	28.308714020726413	22.25694124252383
14-15	23.246827752402698	25.33256677653618	25.56800144933095	25.85260402173017
16-17	22.937090386512736	27.957846992760686	26.476118273892542	22.628944346834036
18-19	22.83153241449886	27.270883708328675	27.464087031670655	22.43349684550181
20-21	24.00503841033013	25.89729852581206	26.988913293887272	23.108749769970537
22-23	24.348333472746152	26.273740133142642	26.351879318822157	23.026047075289043
24-25	23.804442826820175	26.175359538302466	26.497175187348365	23.523022447528994
26-27	23.286998801988634	27.26328666420753	26.73511707541596	22.714597458387875
28-29	23.17272914231992	26.952745007148966	27.191247902643482	22.68327794788763
30-31	23.36668213123831	26.166259242529776	27.148810390886428	23.318248235345482
32-33	23.260556621880998	26.276775105435647	27.602310117905127	22.860358154778226
34-35	23.926918839515242	26.202023911535477	26.664789651936182	23.206267597013095
36-37	23.102074159188614	26.37812111339977	27.059184046536377	23.460620680875234
38-39	23.1279679253921	26.56404924787395	26.570944994789425	23.737037831944527
40-41	23.423016126255007	26.280589166087942	27.13117342762689	23.165221280030163
42-43	23.43652199743266	26.424094147755046	27.128602823747155	23.010781031065136
44-45	23.607278116722618	26.153125445047888	26.73327405482541	23.506322383404086
46-47	23.61047309064464	25.84168680769675	26.610333032391843	23.93750706926677
48-49	23.074487414735735	25.963832488086364	27.072221997802316	23.88945809937558
50-51	23.478794150121633	26.251578065159503	26.659202394660326	23.610425390058538
52-53	23.55331046991066	26.408640496820407	26.278314907264406	23.759734126004524
54-55	23.644006453439836	25.8650082055308	26.596283804461102	23.89470153656827
56-57	23.752072449107423	26.20485651898737	26.228116100404677	23.81495493150053
58-59	23.90922776493332	26.03705409041629	26.668895398200032	23.384822746450357
60-61	23.533373278194187	26.481646860219655	26.459361546159986	23.525618315426172
62-63	23.236871762773248	26.184171118979787	26.946375570939285	23.63258154730768
64-65	23.830722062228197	26.01063495547959	26.332980521351107	23.825662460941103
66-67	23.312480159426404	26.249211477460697	26.33395979901958	24.104348564093314
68-69	23.616994711084526	26.287770535656723	26.891658431213493	23.203576322045254
70-71	23.995893562310215	25.97268794582636	26.62864077075681	23.402777721106617
72-73	23.412366481610388	26.19135248854857	26.7929962061151	23.60328482372594
74-75	23.6625311226684	25.78025988397192	26.667893483968	23.889315509391682
76-77	23.319087540221854	25.367739766327645	26.60938427000035	24.70378842345015
78-79	23.480698604423406	25.296866073614627	26.64792295764303	24.57451236431894
80-81	22.96400788689359	26.339360038650998	26.59215032105794	24.10448175339747
82-83	22.9897917630651	26.335725238558066	26.80357896265902	23.870904035717814
84-85	23.43910475471544	25.563292495077988	27.03224555998735	23.965357190219223
86-87	23.050809005763863	26.60085376691396	26.639535234818545	23.708801992503627
88-89	23.478059691870282	25.691594451739157	26.16605448211309	24.66429137427748
90-91	23.393678599437077	25.923764865639026	26.24896665804358	24.433589876880315
92-93	23.436154768306984	25.897854810660743	26.729587977081636	23.936402443950637
94-95	23.48005905044341	25.580691255784878	26.54491253010259	24.394337163669125
96-97	23.5519951558398	26.176336897632023	26.615012489870598	23.656655456657578
98-99	22.375393037172564	26.382434043608864	27.08592263869632	24.156250280522247
100-101	23.16073090197465	25.823730510417104	26.576264100052548	24.439274487555696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	4.0
3	6.5
4	6.0
5	4.5
6	6.5
7	16.5
8	35.0
9	46.5
10	40.5
11	35.5
12	43.5
13	50.0
14	54.5
15	68.0
16	76.5
17	76.5
18	91.5
19	102.5
20	109.5
21	139.5
22	188.0
23	250.0
24	316.0
25	433.0
26	632.0
27	854.0
28	1072.5
29	1573.0
30	2750.5
31	5088.0
32	9116.0
33	15177.0
34	23104.0
35	31843.5
36	43137.0
37	52188.5
38	52654.5
39	49391.5
40	45490.5
41	40790.5
42	36787.5
43	34542.0
44	34031.0
45	35015.5
46	36135.5
47	37632.0
48	40400.5
49	45462.5
50	50557.0
51	49786.5
52	47396.0
53	48836.5
54	52076.0
55	52000.0
56	43965.0
57	36565.5
58	34465.0
59	33346.5
60	27985.0
61	20436.0
62	16303.5
63	11567.0
64	6802.0
65	4293.5
66	3271.0
67	2769.0
68	2103.5
69	1303.0
70	774.5
71	504.0
72	357.0
73	268.5
74	211.5
75	148.0
76	94.0
77	56.0
78	33.5
79	16.0
80	10.5
81	8.0
82	4.5
83	1.5
84	2.0
85	2.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46776829963938105
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.304420704214893E-4
22-23	0.0012649003217743205
24-25	1.6321294474507363E-4
26-27	0.0023665876988035674
28-29	0.0033458653672740093
30-31	9.384744322841733E-4
32-33	8.160647237253682E-5
34-35	0.003509078312019083
36-37	0.0023665876988035674
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008446269890557561
46-47	0.07364984131621448
48-49	9.384744322841733E-4
50-51	0.0
52-53	4.080323618626841E-5
54-55	0.0
56-57	0.007466992222087119
58-59	0.039252713211190206
60-61	0.030276001250211154
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.6321294474507363E-4
78-79	0.007589401930645924
80-81	0.00563084659370504
82-83	8.160647237253681E-4
84-85	0.0024889974073623727
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06499955524472557
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1225393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.1681048650614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.27643986061902	27.023670912693415
2	16.32461120041636	13.114573891593603
3	6.4253245128643055	7.74279326474349
4	3.2036682017254687	5.147411211190852
5	1.858921580449397	3.733467848970853
6	1.1295153380916145	2.7222294328295553
7	0.7465828941438409	2.0992173987701586
8	0.513284393190796	1.6494129065029843
9	0.40255151555414664	1.4552758341331522
>10	1.825936348706298	12.813920692121183
>50	0.13490167842754183	3.799644841145737
>100	0.13710656841896124	11.499820431633355
>500	0.016070352730643007	4.215707222503617
>1k	0.005085554661595888	2.982854111168076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2209	0.18026869747093383	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	2096	0.17104716609283716	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2062	0.1682725460321709	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1791	0.14615719201921343	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1758	0.14346417843091971	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1724	0.14068955837025346	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1547	0.12624521276031445	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1519	0.1239602315338834	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1487	0.12134882441796224	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1461	0.11922705613627628	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1454	0.11865581082966853	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1438	0.11735010727170793	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1385	0.11302496423596348	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1383	0.1128617512912184	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1366	0.11147444126088529	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1319	0.10763893705937606	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	1273	0.10388503933023938	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1254	0.10233451635516116	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1235	0.10078399338008295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.160647237253682E-5	0.0
12-13	0.0	0.0	0.0	8.160647237253682E-5	0.0
14-15	0.0	0.0	0.0	8.160647237253682E-5	0.0
16-17	0.0	0.0	0.0	2.4481941711761043E-4	0.0
18-19	0.0	0.0	0.0	2.8562265330387885E-4	0.0
20-21	0.0	0.0	0.0	4.4883559804895245E-4	0.0
22-23	0.0	0.0	0.0	7.344582513528312E-4	0.0
24-25	0.0	0.0	0.0	8.160647237253681E-4	0.0
26-27	0.0	0.0	0.0	0.0010608841408429786	0.0
28-29	0.0	0.0	0.0	0.001917752100754615	0.0
30-31	0.0	0.0	0.0	0.004814781869979672	0.0
32-33	0.0	0.0	0.0	0.01256739674537067	0.0
34-35	0.0	0.0	0.0	0.021870534595839868	0.0
36-37	0.0	0.0	0.0	0.03786540318085708	0.0
38-39	0.0	0.0	0.0	0.07005915653182285	0.0
40-41	0.0	0.0	0.0	0.11033195064766976	0.0
42-43	0.0	0.0	0.0	0.14783012470285042	0.0
44-45	0.0	0.0	0.0	0.189571835321403	0.0
46-47	0.0	0.0	0.0	0.24098391291610122	0.0
48-49	0.0	0.0	0.0	0.30210716072313126	0.0
50-51	0.0	0.0	0.0	0.36673948684218044	0.0
52-53	0.0	0.0	0.0	0.42953566733284754	0.0
54-55	0.0	0.0	0.0	0.49653458115070026	0.0
56-57	0.0	0.0	0.0	0.576916956437649	0.0
58-59	0.0	0.0	0.0	0.6651335530723612	0.0
60-61	0.0	0.0	0.0	0.7669376273571009	0.0
62-63	0.0	0.0	0.0	0.8733932705670753	0.0
64-65	0.0	0.0	0.0	0.9874383157076954	0.0
66-67	0.0	0.0	0.0	1.1160501161668135	0.0
68-69	0.0	0.0	0.0	1.264696305593389	0.0
70-71	0.0	0.0	0.0	1.4278276438660904	0.0
72-73	0.0	0.0	0.0	1.5996092682102803	0.0
74-75	0.0	0.0	0.0	1.771635711971588	0.0
76-77	0.0	0.0	0.0	1.9529652935833648	0.0
78-79	0.0	0.0	0.0	2.167182283561274	0.0
80-81	0.0	0.0	0.0	2.418815841121991	0.0
82-83	0.0	0.0	0.0	2.684771334584089	0.0
84-85	0.0	0.0	0.0	2.957214542599803	0.0
86-87	0.0	0.0	0.0	3.236063858696761	0.0
88-89	0.0	0.0	0.0	3.5473517475618026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1080	0.0	53.862305	1
CCGTGCG	230	0.0	51.620346	9
GTATCAA	2155	0.0	47.3495	1
AAACCGT	295	0.0	41.856224	6
AACCGTG	335	0.0	36.858467	7
ATCAACG	2905	0.0	34.821075	3
ACCGTGC	360	0.0	34.298847	8
TCAACGC	3025	0.0	33.596737	4
AAATGCG	255	0.0	29.7981	6
CGCAGAG	3640	0.0	28.050835	8
TATCAAC	3695	0.0	28.018879	2
ACGCAGA	3680	0.0	27.874987	7
CAACGCA	3725	0.0	27.41075	5
GTACATG	5930	0.0	26.936365	1
AACGCAG	3850	0.0	26.644142	6
AATGCGG	270	0.0	26.383732	7
GCCCATA	950	0.0	25.597378	1
ATAACGC	130	1.0036985E-5	25.571926	4
TACATGG	6230	0.0	25.308056	2
GTCTAAT	705	0.0	25.024273	1
>>END_MODULE