FastQCFastQC Report
Fri 10 Feb 2017
SRR3224495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743470
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG17350.23336516604570462No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA15340.20632977793320512No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12090.1626158419304074No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA9440.1269721710358185No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA8680.11674983523208737No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT8570.11527028662891577No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA8310.11177317174869196No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA8110.10908308337928901No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG7840.10545146408059505No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC7750.10424092431436371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11350.049.8321531
GGTATCA5650.049.632041
ATCAACG15000.037.346723
TCAACGC16000.035.012554
GTCTAAT3400.033.5498731
CCGATCG600.003940879431.681729
CGCAGAG17250.031.6497638
TATCAAC17800.031.2052442
GTTATTA3550.030.758228
ACGCAGA18300.029.8337947
CAACGCA20150.027.5659225
GTACATG26950.027.159421
AACGCAG20450.026.929386
TACATGG26800.026.7487742
TCTAATG4150.026.3112492
GGCCGAT1109.50666E-525.8952627
AAACCGT1658.445386E-825.8952626
TATACCG1109.50666E-525.8952625
GCCCATA5600.025.4619561
ACCGTGC2101.2714736E-924.867678
Produced by FastQC (version 0.11.5)