##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224495_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743470
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21678077124834	34.0	31.0	34.0	31.0	34.0
2	32.374278720055955	34.0	31.0	34.0	31.0	34.0
3	32.51643374984868	34.0	31.0	34.0	31.0	34.0
4	35.80409296945405	37.0	37.0	37.0	35.0	37.0
5	35.81334687344479	37.0	37.0	37.0	35.0	37.0
6	35.914869463461876	37.0	37.0	37.0	35.0	37.0
7	35.87756735308755	37.0	37.0	37.0	35.0	37.0
8	35.909568644329966	37.0	37.0	37.0	35.0	37.0
9	37.64600185616097	39.0	39.0	39.0	35.0	39.0
10-11	37.719192435471506	39.0	39.0	39.0	35.0	39.0
12-13	37.72148842589479	39.0	39.0	39.0	35.0	39.0
14-15	39.220121861003136	41.0	40.0	41.0	36.0	41.0
16-17	39.04915867486247	41.0	40.0	41.0	36.0	41.0
18-19	39.12570715698011	41.0	40.0	41.0	36.0	41.0
20-21	39.189852986670616	41.0	40.0	41.0	36.0	41.0
22-23	39.143678964854	41.0	40.0	41.0	36.0	41.0
24-25	39.083453266439804	41.0	40.0	41.0	36.0	41.0
26-27	38.9183928067037	41.0	39.0	41.0	35.0	41.0
28-29	38.83552261691797	41.0	39.0	41.0	35.0	41.0
30-31	38.76575517505751	41.0	39.0	41.0	35.0	41.0
32-33	38.567856134074006	41.0	39.0	41.0	35.0	41.0
34-35	38.56835043781189	40.5	39.0	41.0	35.0	41.0
36-37	38.553563694567366	40.0	39.0	41.0	35.0	41.0
38-39	38.434400850067924	40.0	38.0	41.0	34.5	41.0
40-41	38.337931591052765	40.0	38.0	41.0	34.0	41.0
42-43	38.1892739451491	40.0	38.0	41.0	33.5	41.0
44-45	38.01167162091275	40.0	38.0	41.0	33.0	41.0
46-47	37.828754354580546	40.0	37.5	41.0	33.0	41.0
48-49	37.616123044642016	40.0	37.0	41.0	33.0	41.0
50-51	36.3224723257159	38.5	35.5	40.0	30.5	40.5
52-53	36.64981371138042	39.0	35.5	40.0	31.5	40.5
54-55	37.090010356840224	39.0	35.5	41.0	32.0	41.0
56-57	36.91627167202442	39.0	35.0	41.0	32.0	41.0
58-59	36.7514775310369	39.0	35.0	41.0	32.0	41.0
60-61	36.47867163436319	38.5	35.0	41.0	31.0	41.0
62-63	36.16608269331647	37.5	35.0	41.0	31.0	41.0
64-65	35.8967819817881	37.0	35.0	40.0	31.0	41.0
66-67	35.626264005272574	36.5	35.0	40.0	30.0	41.0
68-69	35.29756076237105	36.0	35.0	39.0	30.0	41.0
70-71	34.98488035831977	35.5	35.0	39.0	29.5	41.0
72-73	34.65359395806152	35.0	34.5	38.5	29.0	40.5
74-75	34.36416936796374	35.0	34.0	37.0	29.0	40.0
76-77	34.04201447267543	35.0	34.0	37.0	29.0	39.0
78-79	33.80244865293825	35.0	34.0	36.5	29.0	39.0
80-81	33.521765504996836	35.0	34.0	36.0	29.0	38.0
82-83	33.2832770656516	35.0	34.0	36.0	28.0	37.0
84-85	33.07554238906748	35.0	34.0	35.0	27.5	37.0
86-87	32.879499509058874	35.0	34.0	35.0	27.0	36.0
88-89	32.74280804874441	35.0	33.5	35.0	27.0	36.0
90-91	32.58695643401886	35.0	33.0	35.0	27.0	36.0
92-93	32.4602862254025	35.0	33.0	35.0	26.5	35.5
94-95	32.33544258678897	35.0	33.0	35.0	26.0	35.0
96-97	32.213172690222876	35.0	33.0	35.0	25.0	35.0
98-99	32.16447872812622	35.0	33.0	35.0	25.0	35.0
100-101	30.949599849355053	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	121.0
8	425.0
9	309.0
10	349.0
11	731.0
12	1287.0
13	1421.0
14	1505.0
15	1527.0
16	1607.0
17	1759.0
18	2004.0
19	2321.0
20	2710.0
21	3291.0
22	3910.0
23	3993.0
24	3895.0
25	4139.0
26	4324.0
27	4668.0
28	5380.0
29	6095.0
30	7551.0
31	9372.0
32	12270.0
33	17220.0
34	27949.0
35	49669.0
36	107630.0
37	196769.0
38	206241.0
39	51016.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16245364327176	18.160926043422094	12.773913875159797	24.90270643814635
2	16.35291269318197	19.261436238180423	38.290448841244434	26.095202227393173
3	20.200573258602578	24.8007650621613	27.872177589112663	27.12648409012346
4	12.962056303549572	16.06063459184634	37.558341291511425	33.41896781309266
5	14.187526060231079	38.08869221353922	33.863235907299554	13.86054581893015
6	31.798055066108923	35.92236404965903	18.39495877439574	13.88462210983631
7	28.418900560883426	30.457584031635438	21.63436318883075	19.489152218650382
8	26.676261315184203	34.13762492097865	20.059585457382276	19.12652830645487
9	27.045610448303226	15.097313946763153	19.2462372388933	38.610838366040326
10-11	26.58264623992898	24.436426486610085	27.422895342111985	21.558031931348946
12-13	26.92765007330491	22.056101792943895	27.38066095471236	23.63558717903883
14-15	23.85217348013816	23.995000060548243	24.52062609575503	27.632200363558567
16-17	24.14393460650368	26.52364194826425	25.611802163945413	23.720621281286657
18-19	24.055111807116745	26.13519102260359	26.28055843689332	23.52913873338634
20-21	25.166227228904802	24.688082165330414	25.95687881003977	24.18881179572502
22-23	24.955232197017363	25.47447814638079	25.303549593842806	24.266740062759045
24-25	24.23583855464638	25.339561914178766	25.88257847103811	24.542021060136747
26-27	24.240251792271376	26.041736721051283	25.920144726754273	23.797866759923064
28-29	23.961356880573526	26.22708380970315	25.970651135889817	23.840908173833512
30-31	24.17152003443313	25.51878354203936	25.94738187149448	24.362314552033034
32-33	23.967745840450856	25.710048825103904	26.03339744710614	24.288807887339097
34-35	24.657820759411948	25.243116736384792	25.748248079949427	24.350814424253837
36-37	24.013332257066367	25.328395958617534	26.435874668711573	24.222397115604526
38-39	24.117531536427297	25.688030819476314	25.640211668679868	24.554225975416518
40-41	24.64403052079725	25.332732512585288	26.02694950079194	23.996287465825517
42-43	24.432810946846246	25.45036835174532	26.104685337354244	24.012135364054195
44-45	24.612147879508576	25.237325276537586	25.736189262956543	24.414337580997298
46-47	24.353302755995536	25.256768934859508	25.635466125062205	24.754462184082747
48-49	23.943131531870822	25.584892463717434	25.99008702435875	24.481888980052997
50-51	24.15006657968714	25.75571307517452	25.7278706605512	24.36634968458714
52-53	24.464605162279582	25.74629776588161	25.101349079317252	24.687747992521555
54-55	24.379665622015683	25.144928510901583	25.641249815056426	24.83415605202631
56-57	24.243159941329136	25.56930714709884	25.506224532411885	24.681308379160143
58-59	24.5770543937107	25.644709547754918	25.77741814845606	24.00081791007832
60-61	24.50192543763768	25.825684833605195	25.400104259505273	24.27228546925185
62-63	24.096060533285435	25.513194902729257	25.942990378205216	24.447754185780088
64-65	24.85372955981613	25.053287115283172	25.31797336720743	24.775009957693257
66-67	24.024801704747247	25.51685913234392	25.2040127210306	25.254326441878234
68-69	24.11939957227595	25.5875152998776	26.1941974793872	24.098887648459254
70-71	24.530377822911483	25.0786178325958	25.901179603749984	24.489824740742733
72-73	24.35827941947893	25.384615384615383	25.9353437260414	24.321761469864285
74-75	24.438107791840963	25.378562685784228	25.79519012199551	24.388139400379302
76-77	24.394259351419695	24.86798391327155	25.47520411045503	25.26255262485373
78-79	24.418474817279147	24.616027238961077	25.962841041936002	25.002656901823777
80-81	24.274152089596832	25.455278294408814	25.485692983896364	24.784876632097994
82-83	24.016105384767037	25.753341887524716	25.77298916506472	24.45756356264353
84-85	24.24415902074842	25.10072161681992	26.05489454249951	24.60022481993215
86-87	24.22176226049953	25.9854896730668	25.50955041134126	24.28319765509241
88-89	24.394521513734897	25.342270844531573	25.281993771817646	24.981213869915887
90-91	24.2101900547433	25.190256499926022	25.53183047063096	25.06772297469972
92-93	24.104494124340825	25.270479394335986	26.060581196949084	24.564445284374102
94-95	24.43649373881932	25.086755349913243	25.96096681775996	24.515784093507474
96-97	24.630650866880977	25.52907313005232	25.752619473549704	24.087656529516995
98-99	23.773982331357	25.80172005638471	25.751213239645764	24.67308437261253
100-101	24.401360168904233	25.179003233563392	25.42902240887689	24.99061418865548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	2.5
3	3.5
4	2.0
5	3.0
6	5.0
7	12.5
8	26.0
9	27.0
10	30.0
11	34.5
12	34.5
13	36.0
14	47.0
15	57.5
16	55.5
17	60.5
18	87.0
19	102.5
20	112.0
21	140.0
22	178.0
23	220.5
24	278.0
25	370.5
26	506.5
27	738.5
28	1047.5
29	1499.5
30	2312.5
31	3629.5
32	5518.5
33	7507.5
34	9365.5
35	11296.0
36	13543.0
37	15285.0
38	16461.0
39	17718.0
40	18351.0
41	18930.5
42	19986.5
43	21197.5
44	22724.5
45	24830.5
46	27166.0
47	28267.5
48	29634.5
49	32419.5
50	34708.5
51	34597.0
52	33972.0
53	34493.0
54	35458.5
55	35468.5
56	31076.0
57	26682.0
58	24878.0
59	22998.0
60	19420.5
61	15234.5
62	12317.0
63	8806.0
64	5617.0
65	3864.0
66	2902.0
67	2421.5
68	1924.0
69	1364.5
70	927.5
71	649.0
72	508.0
73	370.5
74	267.0
75	195.0
76	139.0
77	90.5
78	64.5
79	49.0
80	27.0
81	16.0
82	12.0
83	8.5
84	8.0
85	11.0
86	9.5
87	5.5
88	7.0
89	7.5
90	5.5
91	6.0
92	4.5
93	4.0
94	2.0
95	0.5
96	2.5
97	3.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15185548845279567
2	0.0
3	1.3450441847014674E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0351056532207083
16-17	0.07014405423218154
18-19	0.02434529974309656
20-21	0.038401011473226894
22-23	0.0631498244717339
24-25	0.016476791262592976
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02152070695522348
38-39	0.005918194412686457
40-41	0.005716437784981237
42-43	0.0030936016248133747
44-45	0.010894857896081887
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	6.725220923507337E-5
58-59	0.015064494868656436
60-61	0.017821835447294446
62-63	0.02817867566949574
64-65	0.04384844042126784
66-67	0.018023592074999664
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01654404347182805
80-81	0.05487780273581987
82-83	0.049026860532368495
84-85	0.028044171251025596
86-87	0.0555503248281706
88-89	0.03167579054971956
90-91	0.0
92-93	0.002891844997108155
94-95	0.0
96-97	0.0
98-99	8.070265108208806E-4
100-101	0.043982944839737984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	743470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.76129107981075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74789898356634	48.45585303143819
2	12.874240580562759	15.645109586941713
3	3.6331601778632807	6.622665093201835
4	1.4454365384445025	3.513063609992819
5	0.6978194919287177	2.120020663512323
6	0.37977702067349434	1.3845445259139297
7	0.2364011847448309	1.0054828838524952
8	0.14993718217214655	0.7288301415718728
9	0.11121226237778246	0.6081660581382655
>10	0.5881579233170494	6.724302789332171
>50	0.06228680000408953	2.698744487797106
>100	0.06876039738957981	8.092511860173419
>500	0.004241712825165879	1.7932717620482246
>1k	6.697441302893493E-4	0.6074335060856241
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1735	0.23336516604570462	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1534	0.20632977793320512	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1209	0.1626158419304074	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	944	0.1269721710358185	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	868	0.11674983523208737	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	857	0.11527028662891577	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	831	0.11177317174869196	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	811	0.10908308337928901	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	784	0.10545146408059505	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	775	0.10424092431436371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	1.3450441847014674E-4	0.0
24-25	0.0	0.0	0.0	5.38017673880587E-4	1.3450441847014674E-4
26-27	0.0	0.0	0.0	6.052698831156603E-4	1.3450441847014674E-4
28-29	0.0	0.0	0.0	0.001479548603171614	1.3450441847014674E-4
30-31	0.0	0.0	0.0	0.004236889181809623	1.3450441847014674E-4
32-33	0.0	0.0	0.0	0.014459224985540774	1.3450441847014674E-4
34-35	0.0	0.0	0.0	0.02407629090615627	1.3450441847014674E-4
36-37	0.0	0.0	0.0	0.041965378562685784	1.3450441847014674E-4
38-39	0.0	0.0	0.0	0.06940427993059572	1.3450441847014674E-4
40-41	0.0	0.0	0.0	0.10457718536053909	1.3450441847014674E-4
42-43	0.0	0.0	0.0	0.13625297591025864	2.690088369402935E-4
44-45	0.0	0.0	0.0	0.1722329078510229	2.690088369402935E-4
46-47	0.0	0.0	0.0	0.21211346792742142	2.690088369402935E-4
48-49	0.0	0.0	0.0	0.2556256473025139	3.3626104617536683E-4
50-51	0.0	0.0	0.0	0.3001466098161325	4.0351325541044023E-4
52-53	0.0	0.0	0.0	0.34816468720997484	4.7076546464551363E-4
54-55	0.0	0.0	0.0	0.3990746096009254	5.38017673880587E-4
56-57	0.0	0.0	0.0	0.45805479710008473	5.38017673880587E-4
58-59	0.0	0.0	0.0	0.5247689886612775	5.38017673880587E-4
60-61	0.0	0.0	0.0	0.5953165561488695	5.38017673880587E-4
62-63	0.0	0.0	0.0	0.6674781766581033	5.38017673880587E-4
64-65	0.0	0.0	0.0	0.7419936244905645	6.052698831156603E-4
66-67	0.0	0.0	0.0	0.8294214964961599	6.725220923507338E-4
68-69	0.0	1.3450441847014674E-4	0.0	0.9305688191857102	6.725220923507338E-4
70-71	0.0	1.3450441847014674E-4	0.0	1.0354150133831896	6.725220923507338E-4
72-73	0.0	1.3450441847014674E-4	0.0	1.1566034944247918	7.397743015858071E-4
74-75	0.0	1.3450441847014674E-4	0.0	1.2908389040579982	8.070265108208805E-4
76-77	0.0	1.3450441847014674E-4	0.0	1.4246708004357944	8.070265108208805E-4
78-79	0.0	1.3450441847014674E-4	0.0	1.5784766029564072	8.070265108208805E-4
80-81	0.0	1.3450441847014674E-4	0.0	1.745127577440919	8.070265108208805E-4
82-83	0.0	1.3450441847014674E-4	0.0	1.930340161674311	8.070265108208805E-4
84-85	0.0	1.3450441847014674E-4	0.0	2.1196551306710427	8.070265108208805E-4
86-87	0.0	1.3450441847014674E-4	0.0	2.3219497760501433	8.070265108208805E-4
88-89	0.0	1.3450441847014674E-4	0.0	2.54791719907999	8.070265108208805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1135	0.0	49.832153	1
GGTATCA	565	0.0	49.63204	1
ATCAACG	1500	0.0	37.34672	3
TCAACGC	1600	0.0	35.01255	4
GTCTAAT	340	0.0	33.549873	1
CCGATCG	60	0.0039408794	31.68172	9
CGCAGAG	1725	0.0	31.649763	8
TATCAAC	1780	0.0	31.205244	2
GTTATTA	355	0.0	30.75822	8
ACGCAGA	1830	0.0	29.833794	7
CAACGCA	2015	0.0	27.565922	5
GTACATG	2695	0.0	27.15942	1
AACGCAG	2045	0.0	26.92938	6
TACATGG	2680	0.0	26.748774	2
TCTAATG	415	0.0	26.311249	2
GGCCGAT	110	9.50666E-5	25.895262	7
AAACCGT	165	8.445386E-8	25.895262	6
TATACCG	110	9.50666E-5	25.895262	5
GCCCATA	560	0.0	25.461956	1
ACCGTGC	210	1.2714736E-9	24.86767	8
>>END_MODULE