FastQCFastQC Report
Fri 10 Feb 2017
SRR3224495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743470
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT10810.14539927636622862No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG10540.14176765706753466No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA10490.14109513497518394No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA10070.13544594939943777No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG9360.12589613568805735No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG8780.11809487941678884No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA8770.11796037499831868No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT7920.10652749942835621No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT7730.10397191547742343No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG7470.10047480059719961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.049.6392631
GGTATCA6050.048.882881
ATCAACG15700.038.111793
TCAACGC16300.036.70894
CAACGCA18300.032.6970025
ACCGTGC2350.032.3325968
TATCAAC19300.031.0028552
AACGCAG19450.030.763766
CGCAGAG19800.030.6996388
CCGTGCG1901.2732926E-1129.9927359
ACGCAGA20400.029.3311337
ACCGTAT1354.2306783E-728.1413358
CGTTACG350.00837886227.15180852-53
GGACCGT1257.400213E-626.593566
AACCGTG2155.820766E-1126.505217
GTACATG29550.026.4732251
GTCTAGG2059.094947E-1025.5952431
GACCGCA5700.024.9939487
GACCGTA950.001227879324.9939467
TACATGG29100.024.968182
Produced by FastQC (version 0.11.5)